(Linear) (Six-base) MAPSORT of: KIEE0879.seq  Check: 6335  from: 1  to: 924



KIEE0879.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AflIII A'CryG_T

Cuts at:      0      74     715     924 
   Size:         74     641     209

  Fragments arranged by size: 

                 641     209      74
 
AleI CACnn'nnGTG

Cuts at:      0      22      88     500     924 
   Size:         22      66     412     424

  Fragments arranged by size: 

                 424     412      66      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     924 
   Size:        403     521
 
ApoI r'AATT_y

Cuts at:      0     583     924 
   Size:        583     341
 
BbvCI CC'TCA_GC

Cuts at:      0     106     924 
   Size:        106     818
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     409     924 
   Size:        409     515
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     558     924 
   Size:        558     366
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673     924 
   Size:        673     251
 
Bpu10I CC'TnA_GC

Cuts at:      0     106     924 
   Size:        106     818
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     924 
   Size:        685     239
 
BsaBI GATnn'nnATC

Cuts at:      0     595     924 
   Size:        595     329
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     631     924 
   Size:        631     293
 
BsiHKAI G_wGCw'C

Cuts at:      0     464     924 
   Size:        464     460
 
Bsp1286I G_dGCh'C

Cuts at:      0     464     924 
   Size:        464     460
 
Bsu36I CC'TnA_GG

Cuts at:      0     487     924 
   Size:        487     437
 
BtgI C'CryG_G

Cuts at:      0       5     350     734     924 
   Size:          5     345     384     190

  Fragments arranged by size: 

                 384     345     190       5
 
BtsI GCAGTG_nn'

Cuts at:      0      29     464     924 
   Size:         29     435     460

  Fragments arranged by size: 

                 460     435      29
 
EaeI y'GGCC_r

Cuts at:      0       2     320     924 
   Size:          2     318     604

  Fragments arranged by size: 

                 604     318       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673     924 
   Size:        673     251
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     924 
   Size:        119      32     773

  Fragments arranged by size: 

                 773     119      32
 
MscI TGG'CCA

Cuts at:      0       4     322     924 
   Size:          4     318     602

  Fragments arranged by size: 

                 602     318       4
 
MslI CAynn'nnrTG

Cuts at:      0      22      88     469     500     766     772     924 
   Size:         22      66     381      31     266       6     152

  Fragments arranged by size: 

                 381     266     152      66      31      22       6
 
NcoI C'CATG_G

Cuts at:      0       5     350     924 
   Size:          5     345     574

  Fragments arranged by size: 

                 574     345       5
 
NspI r_CATG'y

Cuts at:      0     719     924 
   Size:        719     205
 
PciI A'CATG_T

Cuts at:      0     715     924 
   Size:        715     209
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     924 
   Size:        243     681
 
SmlI C'TyrA_G

Cuts at:      0     700     924 
   Size:        700     224
 
StuI AGG'CCT

Cuts at:      0     706     924 
   Size:        706     218
 
StyI C'CwwG_G

Cuts at:      0       5     311     350     924 
   Size:          5     306      39     574

  Fragments arranged by size: 

                 574     306      39       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     858     924 
   Size:        858      66
 
Tth111I GACn'n_nGTC

Cuts at:      0      13     924 
   Size:         13     911
 
XmnI GAAnn'nnTTC

Cuts at:      0     225     924 
   Size:        225     699
 

 Enzymes that do cut:

   AflIII     AleI     AlfI     ApoI    BbvCI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaBI    BseRI  BsiHKAI Bsp1286I   Bsu36I     BtgI
     BtsI     EaeI  Eco57MI    Hin4I     MscI     MslI     NcoI     NspI
     PciI    PflMI     SmlI     StuI     StyI    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI