Restriction map for
pF1KE0882
(Linear) (Six-base) MAPSORT of: KIEE0882.seq Check: 8461 from: 1 to: 954
KIEE0882.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 791 850 947 954
Size: 791 59 97 7
Fragments arranged by size:
791 97 59 7
AclI AA'CG_TT
Cuts at: 0 530 954
Size: 530 424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 824 954
Size: 689 135 130
Fragments arranged by size:
689 135 130
AlwNI CAG_nnn'CTG
Cuts at: 0 249 712 954
Size: 249 463 242
Fragments arranged by size:
463 249 242
ApaI G_GGCC'C
Cuts at: 0 646 954
Size: 646 308
ApaLI G'TGCA_C
Cuts at: 0 914 954
Size: 914 40
AvaI C'yCGr_G
Cuts at: 0 57 954
Size: 57 897
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 329 362 954
Size: 329 33 592
Fragments arranged by size:
592 329 33
BamHI G'GATC_C
Cuts at: 0 742 954
Size: 742 212
BanI G'GyrC_C
Cuts at: 0 775 954
Size: 775 179
BanII G_rGCy'C
Cuts at: 0 646 954
Size: 646 308
BfrBI ATG'CAT
Cuts at: 0 322 954
Size: 322 632
Bme1580I G_kGCm'C
Cuts at: 0 646 654 918 954
Size: 646 8 264 36
Fragments arranged by size:
646 264 36 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 133 237 521 954
Size: 133 104 284 433
Fragments arranged by size:
433 284 133 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 954
Size: 147 807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 465 954
Size: 465 489
BsaI GGTCTCn'nnnn_
Cuts at: 0 267 954
Size: 267 687
BsaWI w'CCGG_w
Cuts at: 0 33 954
Size: 33 921
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 954
Size: 73 881
BseYI C'CCAG_C
Cuts at: 0 449 520 639 954
Size: 449 71 119 315
Fragments arranged by size:
449 315 119 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 379 451 954
Size: 154 225 72 503
Fragments arranged by size:
503 225 154 72
BsiHKAI G_wGCw'C
Cuts at: 0 918 954
Size: 918 36
Bsp1286I G_dGCh'C
Cuts at: 0 646 654 918 954
Size: 646 8 264 36
Fragments arranged by size:
646 264 36 8
BsrDI GCAATG_nn'
Cuts at: 0 347 954
Size: 347 607
BsrFI r'CCGG_y
Cuts at: 0 560 954
Size: 560 394
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 954
Size: 123 831
BstYI r'GATC_y
Cuts at: 0 742 954
Size: 742 212
BstZ17I GTA'TAC
Cuts at: 0 851 954
Size: 851 103
BtgI C'CryG_G
Cuts at: 0 755 954
Size: 755 199
BtsI GCAGTG_nn'
Cuts at: 0 235 954
Size: 235 719
EaeI y'GGCC_r
Cuts at: 0 377 562 608 869 954
Size: 377 185 46 261 85
Fragments arranged by size:
377 261 185 85 46
EarI CTCTTCn'nnn_
Cuts at: 0 91 954
Size: 91 863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 689 824 954
Size: 147 542 135 130
Fragments arranged by size:
542 147 135 130
EcoO109I rG'GnC_Cy
Cuts at: 0 643 954
Size: 643 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 734 766 954
Size: 53 32 649 32 188
Fragments arranged by size:
649 188 53 32 32
HincII GTy'rAC
Cuts at: 0 534 954
Size: 534 420
HpaI GTT'AAC
Cuts at: 0 534 954
Size: 534 420
MscI TGG'CCA
Cuts at: 0 379 610 871 954
Size: 379 231 261 83
Fragments arranged by size:
379 261 231 83
MslI CAynn'nnrTG
Cuts at: 0 599 954
Size: 599 355
MspA1I CmG'CkG
Cuts at: 0 293 709 954
Size: 293 416 245
Fragments arranged by size:
416 293 245
NcoI C'CATG_G
Cuts at: 0 755 954
Size: 755 199
NsiI A_TGCA'T
Cuts at: 0 324 954
Size: 324 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 249 954
Size: 249 705
PspOMI G'GGCC_C
Cuts at: 0 642 954
Size: 642 312
PstI C_TGCA'G
Cuts at: 0 925 954
Size: 925 29
PvuII CAG'CTG
Cuts at: 0 709 954
Size: 709 245
SfcI C'TryA_G
Cuts at: 0 921 954
Size: 921 33
SmlI C'TyrA_G
Cuts at: 0 57 444 954
Size: 57 387 510
Fragments arranged by size:
510 387 57
StyI C'CwwG_G
Cuts at: 0 48 755 954
Size: 48 707 199
Fragments arranged by size:
707 199 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 441 927 954
Size: 192 249 486 27
Fragments arranged by size:
486 249 192 27
TatI w'GTAC_w
Cuts at: 0 660 954
Size: 660 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 864 954
Size: 480 384 90
Fragments arranged by size:
480 384 90
XhoI C'TCGA_G
Cuts at: 0 57 954
Size: 57 897
Enzymes that do cut:
AccI AclI AcuI AlwNI ApaI ApaLI AvaI BaeI
BamHI BanI BanII BfrBI Bme1580I BmrI BpmI BpuEI
BsaI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BsrFI BstXI BstYI BstZ17I BtgI BtsI EaeI EarI
Eco57MI EcoO109I Hin4I HincII HpaI MscI MslI MspA1I
NcoI NsiI PflMI PspOMI PstI PvuII SfcI SmlI
StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XmaI XmnI ZraI