Restriction map for
pF1KE0885
(Linear) (Six-base) MAPSORT of: KIEE0885.seq Check: 3816 from: 1 to: 990
KIEE0885.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 224 439 990
Size: 224 215 551
Fragments arranged by size:
551 224 215
AflIII A'CryG_T
Cuts at: 0 593 990
Size: 593 397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 327 990
Size: 295 32 663
Fragments arranged by size:
663 295 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 990
Size: 132 858
AvaI C'yCGr_G
Cuts at: 0 566 990
Size: 566 424
BanII G_rGCy'C
Cuts at: 0 466 473 951 990
Size: 466 7 478 39
Fragments arranged by size:
478 466 39 7
BfrBI ATG'CAT
Cuts at: 0 942 990
Size: 942 48
BglI GCCn_nnn'nGGC
Cuts at: 0 406 990
Size: 406 584
Bme1580I G_kGCm'C
Cuts at: 0 9 990
Size: 9 981
Bpu10I CC'TnA_GC
Cuts at: 0 116 990
Size: 116 874
BsaI GGTCTCn'nnnn_
Cuts at: 0 372 801 963 990
Size: 372 429 162 27
Fragments arranged by size:
429 372 162 27
BsaAI yAC'GTr
Cuts at: 0 712 990
Size: 712 278
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 514 517 990
Size: 475 39 3 473
Fragments arranged by size:
475 473 39 3
BseYI C'CCAG_C
Cuts at: 0 171 990
Size: 171 819
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 990
Size: 202 788
BsiHKAI G_wGCw'C
Cuts at: 0 185 990
Size: 185 805
Bsp1286I G_dGCh'C
Cuts at: 0 9 185 466 473 951 990
Size: 9 176 281 7 478 39
Fragments arranged by size:
478 281 176 39 9 7
BspHI T'CATG_A
Cuts at: 0 692 990
Size: 692 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 439 839 990
Size: 224 215 400 151
Fragments arranged by size:
400 224 215 151
BsrGI T'GTAC_A
Cuts at: 0 596 990
Size: 596 394
BstEII G'GTnAC_C
Cuts at: 0 375 549 990
Size: 375 174 441
Fragments arranged by size:
441 375 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 297 990
Size: 159 138 693
Fragments arranged by size:
693 159 138
BstYI r'GATC_y
Cuts at: 0 101 128 572 990
Size: 101 27 444 418
Fragments arranged by size:
444 418 101 27
Bsu36I CC'TnA_GG
Cuts at: 0 684 925 990
Size: 684 241 65
Fragments arranged by size:
684 241 65
EaeI y'GGCC_r
Cuts at: 0 194 242 398 425 990
Size: 194 48 156 27 565
Fragments arranged by size:
565 194 156 48 27
EarI CTCTTCn'nnn_
Cuts at: 0 271 589 951 990
Size: 271 318 362 39
Fragments arranged by size:
362 318 271 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 978 990
Size: 978 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 990
Size: 647 343
MscI TGG'CCA
Cuts at: 0 196 244 427 990
Size: 196 48 183 563
Fragments arranged by size:
563 196 183 48
MslI CAynn'nnrTG
Cuts at: 0 226 457 788 877 990
Size: 226 231 331 89 113
Fragments arranged by size:
331 231 226 113 89
NsiI A_TGCA'T
Cuts at: 0 944 990
Size: 944 46
NspI r_CATG'y
Cuts at: 0 597 620 942 990
Size: 597 23 322 48
Fragments arranged by size:
597 322 48 23
PasI CC'CwG_GG
Cuts at: 0 466 990
Size: 466 524
PciI A'CATG_T
Cuts at: 0 593 990
Size: 593 397
PfoI T'CCnGG_A
Cuts at: 0 951 990
Size: 951 39
PstI C_TGCA'G
Cuts at: 0 836 990
Size: 836 154
SexAI A'CCwGG_T
Cuts at: 0 158 990
Size: 158 832
SfcI C'TryA_G
Cuts at: 0 832 918 990
Size: 832 86 72
Fragments arranged by size:
832 86 72
SmlI C'TyrA_G
Cuts at: 0 566 990
Size: 566 424
SnaBI TAC'GTA
Cuts at: 0 712 990
Size: 712 278
StuI AGG'CCT
Cuts at: 0 984 990
Size: 984 6
StyI C'CwwG_G
Cuts at: 0 371 990
Size: 371 619
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 778 990
Size: 643 135 212
Fragments arranged by size:
643 212 135
TatI w'GTAC_w
Cuts at: 0 230 596 990
Size: 230 366 394
Fragments arranged by size:
394 366 230
XhoI C'TCGA_G
Cuts at: 0 566 990
Size: 566 424
XmnI GAAnn'nnTTC
Cuts at: 0 910 990
Size: 910 80
Enzymes that do cut:
AarI AflIII AloI AlwNI AvaI BanII BfrBI BglI
Bme1580I Bpu10I BsaI BsaAI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrGI BstEII BstXI BstYI Bsu36I
EaeI EarI EcoNI MmeI MscI MslI NsiI NspI
PasI PciI PfoI PstI SexAI SfcI SmlI SnaBI
StuI StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BplI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XmaI ZraI