Restriction map for
pF1KE0886
(Linear) (Six-base) MAPSORT of: KIEE0886.seq Check: 6777 from: 1 to: 969
KIEE0886.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 969
Size: 601 368
AleI CACnn'nnGTG
Cuts at: 0 172 865 969
Size: 172 693 104
Fragments arranged by size:
693 172 104
AvaI C'yCGr_G
Cuts at: 0 602 969
Size: 602 367
BanII G_rGCy'C
Cuts at: 0 439 641 927 969
Size: 439 202 286 42
Fragments arranged by size:
439 286 202 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 969
Size: 688 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 969
Size: 802 167
BsaBI GATnn'nnATC
Cuts at: 0 625 969
Size: 625 344
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 684 969
Size: 118 566 285
Fragments arranged by size:
566 285 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 554 969
Size: 400 154 415
Fragments arranged by size:
415 400 154
Bsp1286I G_dGCh'C
Cuts at: 0 439 641 927 969
Size: 439 202 286 42
Fragments arranged by size:
439 286 202 42
BspHI T'CATG_A
Cuts at: 0 431 969
Size: 431 538
BsrGI T'GTAC_A
Cuts at: 0 857 969
Size: 857 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 252 969
Size: 252 717
BstEII G'GTnAC_C
Cuts at: 0 867 969
Size: 867 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 969
Size: 117 852
BstYI r'GATC_y
Cuts at: 0 324 969
Size: 324 645
Bsu36I CC'TnA_GG
Cuts at: 0 901 969
Size: 901 68
BtgI C'CryG_G
Cuts at: 0 350 455 969
Size: 350 105 514
Fragments arranged by size:
514 350 105
EaeI y'GGCC_r
Cuts at: 0 95 347 371 515 969
Size: 95 252 24 144 454
Fragments arranged by size:
454 252 144 95 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 969
Size: 601 368
EcoO109I rG'GnC_Cy
Cuts at: 0 674 708 969
Size: 674 34 261
Fragments arranged by size:
674 261 34
HaeII r_GCGC'y
Cuts at: 0 466 969
Size: 466 503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 969
Size: 81 888
MscI TGG'CCA
Cuts at: 0 97 349 373 517 969
Size: 97 252 24 144 452
Fragments arranged by size:
452 252 144 97 24
MslI CAynn'nnrTG
Cuts at: 0 172 844 850 865 969
Size: 172 672 6 15 104
Fragments arranged by size:
672 172 104 15 6
NcoI C'CATG_G
Cuts at: 0 350 969
Size: 350 619
NspI r_CATG'y
Cuts at: 0 255 969
Size: 255 714
PpuMI rG'GwC_Cy
Cuts at: 0 674 708 969
Size: 674 34 261
Fragments arranged by size:
674 261 34
PspXI vC'TCGA_Gb
Cuts at: 0 602 969
Size: 602 367
PstI C_TGCA'G
Cuts at: 0 248 969
Size: 248 721
SfcI C'TryA_G
Cuts at: 0 244 894 969
Size: 244 650 75
Fragments arranged by size:
650 244 75
SmlI C'TyrA_G
Cuts at: 0 602 703 969
Size: 602 101 266
Fragments arranged by size:
602 266 101
StuI AGG'CCT
Cuts at: 0 736 969
Size: 736 233
StyI C'CwwG_G
Cuts at: 0 350 669 969
Size: 350 319 300
Fragments arranged by size:
350 319 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 462 606 969
Size: 462 144 363
Fragments arranged by size:
462 363 144
TatI w'GTAC_w
Cuts at: 0 302 857 969
Size: 302 555 112
Fragments arranged by size:
555 302 112
Tth111I GACn'n_nGTC
Cuts at: 0 841 969
Size: 841 128
XbaI T'CTAG_A
Cuts at: 0 327 969
Size: 327 642
XhoI C'TCGA_G
Cuts at: 0 602 969
Size: 602 367
Enzymes that do cut:
AcuI AleI AvaI BanII BpuEI BsaI BsaBI BseRI
BsgI Bsp1286I BspHI BsrGI BstAPI BstEII BstXI BstYI
Bsu36I BtgI EaeI Eco57MI EcoO109I HaeII MmeI MscI
MslI NcoI NspI PpuMI PspXI PstI SfcI SmlI
StuI StyI TaqII TatI Tth111I XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI XcmI XmaI XmnI ZraI