Restriction map for
pF1KE0896
(Linear) (Six-base) MAPSORT of: KIEE0896.seq Check: 8026 from: 1 to: 990
KIEE0896.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 990
Size: 625 365
AleI CACnn'nnGTG
Cuts at: 0 166 990
Size: 166 824
ApaLI G'TGCA_C
Cuts at: 0 473 990
Size: 473 517
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 614 647 990
Size: 614 33 343
Fragments arranged by size:
614 343 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 687 990
Size: 687 303
BclI T'GATC_A
Cuts at: 0 644 990
Size: 644 346
Bme1580I G_kGCm'C
Cuts at: 0 477 561 990
Size: 477 84 429
Fragments arranged by size:
477 429 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 990
Size: 764 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 990
Size: 945 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 815 990
Size: 815 175
BsaBI GATnn'nnATC
Cuts at: 0 646 990
Size: 646 344
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 990
Size: 130 860
BseYI C'CCAG_C
Cuts at: 0 464 483 494 990
Size: 464 19 11 496
Fragments arranged by size:
496 464 19 11
BsiHKAI G_wGCw'C
Cuts at: 0 477 600 990
Size: 477 123 390
Fragments arranged by size:
477 390 123
Bsp1286I G_dGCh'C
Cuts at: 0 477 561 600 990
Size: 477 84 39 390
Fragments arranged by size:
477 390 84 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 990
Size: 185 805
BstEII G'GTnAC_C
Cuts at: 0 159 990
Size: 159 831
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 990
Size: 294 696
BtgI C'CryG_G
Cuts at: 0 164 653 990
Size: 164 489 337
Fragments arranged by size:
489 337 164
EaeI y'GGCC_r
Cuts at: 0 109 191 239 296 422 990
Size: 109 82 48 57 126 568
Fragments arranged by size:
568 126 109 82 57 48
EarI CTCTTCn'nnn_
Cuts at: 0 131 805 990
Size: 131 674 185
Fragments arranged by size:
674 185 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 764 990
Size: 625 139 226
Fragments arranged by size:
625 226 139
FspI TGC'GCA
Cuts at: 0 19 990
Size: 19 971
HindIII A'AGCT_T
Cuts at: 0 607 990
Size: 607 383
MscI TGG'CCA
Cuts at: 0 111 193 241 298 424 990
Size: 111 82 48 57 126 566
Fragments arranged by size:
566 126 111 82 57 48
MslI CAynn'nnrTG
Cuts at: 0 166 454 652 990
Size: 166 288 198 338
Fragments arranged by size:
338 288 198 166
NcoI C'CATG_G
Cuts at: 0 164 653 990
Size: 164 489 337
Fragments arranged by size:
489 337 164
NdeI CA'TA_TG
Cuts at: 0 408 990
Size: 408 582
NspI r_CATG'y
Cuts at: 0 153 990
Size: 153 837
PfoI T'CCnGG_A
Cuts at: 0 741 990
Size: 741 249
SapI GCTCTTCn'nnn_
Cuts at: 0 805 990
Size: 805 185
SexAI A'CCwGG_T
Cuts at: 0 155 990
Size: 155 835
SfcI C'TryA_G
Cuts at: 0 851 990
Size: 851 139
SmlI C'TyrA_G
Cuts at: 0 924 990
Size: 924 66
StuI AGG'CCT
Cuts at: 0 760 990
Size: 760 230
StyI C'CwwG_G
Cuts at: 0 144 164 653 990
Size: 144 20 489 337
Fragments arranged by size:
489 337 144 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 326 475 486 808 990
Size: 326 149 11 322 182
Fragments arranged by size:
326 322 182 149 11
TatI w'GTAC_w
Cuts at: 0 227 990
Size: 227 763
XmnI GAAnn'nnTTC
Cuts at: 0 760 990
Size: 760 230
Enzymes that do cut:
AcuI AleI ApaLI BaeI BbsI BclI Bme1580I BpmI
BpuEI BsaI BsaBI BseRI BseYI BsiHKAI Bsp1286I BspMI
BstEII BstXI BtgI EaeI EarI Eco57MI FspI HindIII
MscI MslI NcoI NdeI NspI PfoI SapI SexAI
SfcI SmlI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI