Restriction map for
pF1KE0900
(Linear) (Six-base) MAPSORT of: KIEE0900.seq Check: 5355 from: 1 to: 10254
KIEE0900.orf
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5532 10254
Size: 5532 4722
AccI GT'mk_AC
Cuts at: 0 336 3527 4863 7621 8365 9825 10254
Size: 336 3191 1336 2758 744 1460 429
Fragments arranged by size:
3191 2758 1460 1336 744 429 336
AclI AA'CG_TT
Cuts at: 0 2489 10254
Size: 2489 7765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 739 929 1088 1307 1619 1976 2657
Size: 92 647 190 159 219 312 357 681
Cuts at: 2657 3009 3713 5846 6410 7435 7712 9153 9581
Size: 352 704 2133 564 1025 277 1441 428
Cuts at: 9581 10254
Size: 673
Fragments arranged by size:
2133 1441 1025 704 681 673 647 564
428 357 352 312 277 219 190 159
92
AflII C'TTAA_G
Cuts at: 0 4845 10254
Size: 4845 5409
AflIII A'CryG_T
Cuts at: 0 447 2023 6763 7503 10254
Size: 447 1576 4740 740 2751
Fragments arranged by size:
4740 2751 1576 740 447
AgeI A'CCGG_T
Cuts at: 0 9114 10254
Size: 9114 1140
AhdI GACnn_n'nnGTC
Cuts at: 0 2900 8240 10254
Size: 2900 5340 2014
Fragments arranged by size:
5340 2900 2014
AleI CACnn'nnGTG
Cuts at: 0 8902 10254
Size: 8902 1352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 8702 8717 10254
Size: 8702 15 1537
Fragments arranged by size:
8702 1537 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 308 340 7574 7606 10254
Size: 308 32 7234 32 2648
Fragments arranged by size:
7234 2648 308 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2116 2635 7399 10254
Size: 2116 519 4764 2855
Fragments arranged by size:
4764 2855 2116 519
ApaLI G'TGCA_C
Cuts at: 0 8663 10254
Size: 8663 1591
ApoI r'AATT_y
Cuts at: 0 38 298 405 813 992 1329 1665 1707
Size: 38 260 107 408 179 337 336 42
Cuts at: 1707 3376 3987 4003 4341 4386 4785 4995 5648
Size: 1669 611 16 338 45 399 210 653
Cuts at: 5648 5783 6150 6244 6385 6795 6874 7041 7063
Size: 135 367 94 141 410 79 167 22
Cuts at: 7063 7795 7850 7921 8603 8614 9020 9128 9175
Size: 732 55 71 682 11 406 108 47
Cuts at: 9175 9951 9979 9989 10254
Size: 776 28 10 265
Fragments arranged by size:
1669 776 732 682 653 611 410 408
406 399 367 338 337 336 265 260
210 179 167 141 135 108 107 94
79 71 55 47 45 42 38 28
22 16 11 10
AseI AT'TA_AT
Cuts at: 0 3194 7346 10254
Size: 3194 4152 2908
Fragments arranged by size:
4152 3194 2908
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6980 7013 10254
Size: 6980 33 3241
Fragments arranged by size:
6980 3241 33
BamHI G'GATC_C
Cuts at: 0 10 564 7830 10254
Size: 10 554 7266 2424
Fragments arranged by size:
7266 2424 554 10
BanI G'GyrC_C
Cuts at: 0 4830 9913 10254
Size: 4830 5083 341
Fragments arranged by size:
5083 4830 341
BanII G_rGCy'C
Cuts at: 0 1218 6827 7809 8281 9161 10254
Size: 1218 5609 982 472 880 1093
Fragments arranged by size:
5609 1218 1093 982 880 472
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 2664 6265 7441 8503 9644 10254
Size: 340 2324 3601 1176 1062 1141 610
Fragments arranged by size:
3601 2324 1176 1141 1062 610 340
BbvCI CC'TCA_GC
Cuts at: 0 557 10254
Size: 557 9697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 10012 10046 10254
Size: 10012 34 208
Fragments arranged by size:
10012 208 34
BclI T'GATC_A
Cuts at: 0 2283 4077 4350 10169 10254
Size: 2283 1794 273 5819 85
Fragments arranged by size:
5819 2283 1794 273 85
BfrBI ATG'CAT
Cuts at: 0 5395 6966 9543 10254
Size: 5395 1571 2577 711
Fragments arranged by size:
5395 2577 1571 711
Bme1580I G_kGCm'C
Cuts at: 0 8667 10254
Size: 8667 1587
BmrI ACTGGGnnnn_n'
Cuts at: 0 1848 2212 4480 8184 10254
Size: 1848 364 2268 3704 2070
Fragments arranged by size:
3704 2268 2070 1848 364
BmtI G_CTAG'C
Cuts at: 0 4583 10254
Size: 4583 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3200 10254
Size: 3200 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 6089 6914 7805 9358 9440 10254
Size: 1460 4629 825 891 1553 82 814
Fragments arranged by size:
4629 1553 1460 891 825 814 82
Bpu10I CC'TnA_GC
Cuts at: 0 557 4838 5004 7939 10254
Size: 557 4281 166 2935 2315
Fragments arranged by size:
4281 2935 2315 557 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4169 8770 9840 10254
Size: 4169 4601 1070 414
Fragments arranged by size:
4601 4169 1070 414
BsaI GGTCTCn'nnnn_
Cuts at: 0 1190 4735 10254
Size: 1190 3545 5519
Fragments arranged by size:
5519 3545 1190
BsaAI yAC'GTr
Cuts at: 0 1342 6100 7625 8662 10254
Size: 1342 4758 1525 1037 1592
Fragments arranged by size:
4758 1592 1525 1342 1037
BsaBI GATnn'nnATC
Cuts at: 0 1088 3448 3485 10254
Size: 1088 2360 37 6769
Fragments arranged by size:
6769 2360 1088 37
BsaHI Gr'CG_yC
Cuts at: 0 8970 10254
Size: 8970 1284
BsaWI w'CCGG_w
Cuts at: 0 9114 10254
Size: 9114 1140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 1132 1162 7574 7604 8426 8456
Size: 545 30 557 30 6412 30 822 30
Cuts at: 8456 10254
Size: 1798
Fragments arranged by size:
6412 1798 822 557 545 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2872 6833 9947 10254
Size: 2872 3961 3114 307
Fragments arranged by size:
3961 3114 2872 307
BseYI C'CCAG_C
Cuts at: 0 8739 10254
Size: 8739 1515
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3191 8150 8723 9863 10254
Size: 3191 4959 573 1140 391
Fragments arranged by size:
4959 3191 1140 573 391
BsiHKAI G_wGCw'C
Cuts at: 0 626 6347 8667 10254
Size: 626 5721 2320 1587
Fragments arranged by size:
5721 2320 1587 626
BsmI GAATG_Cn'
Cuts at: 0 1439 1731 1917 3478 3510 5745 7461 8733
Size: 1439 292 186 1561 32 2235 1716 1272
Cuts at: 8733 10254
Size: 1521
Fragments arranged by size:
2235 1716 1561 1521 1439 1272 292 186
32
Bsp1286I G_dGCh'C
Cuts at: 0 626 1218 6347 6827 7809 8281 8667 9161
Size: 626 592 5129 480 982 472 386 494
Cuts at: 9161 10254
Size: 1093
Fragments arranged by size:
5129 1093 982 626 592 494 480 472
386
BspHI T'CATG_A
Cuts at: 0 666 705 2286 2348 2727 3795 10254
Size: 666 39 1581 62 379 1068 6459
Fragments arranged by size:
6459 1581 1068 666 379 62 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 1720 5532 5662 6382 10254
Size: 144 1576 3812 130 720 3872
Fragments arranged by size:
3872 3812 1576 720 144 130
BsrDI GCAATG_nn'
Cuts at: 0 7641 8532 9525 10254
Size: 7641 891 993 729
Fragments arranged by size:
7641 993 891 729
BsrFI r'CCGG_y
Cuts at: 0 9114 10254
Size: 9114 1140
BsrGI T'GTAC_A
Cuts at: 0 442 8293 9859 10254
Size: 442 7851 1566 395
Fragments arranged by size:
7851 1566 442 395
BssSI C'ACGA_G
Cuts at: 0 6320 10254
Size: 6320 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9549 10254
Size: 9549 705
BstYI r'GATC_y
Cuts at: 0 10 564 2052 7830 8196 8828 9183 10254
Size: 10 554 1488 5778 366 632 355 1071
Fragments arranged by size:
5778 1488 1071 632 554 366 355 10
BstZ17I GTA'TAC
Cuts at: 0 7622 8366 10254
Size: 7622 744 1888
Fragments arranged by size:
7622 1888 744
Bsu36I CC'TnA_GG
Cuts at: 0 7916 10254
Size: 7916 2338
BtgI C'CryG_G
Cuts at: 0 2441 2777 8852 9151 10254
Size: 2441 336 6075 299 1103
Fragments arranged by size:
6075 2441 1103 336 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8402 10254
Size: 8402 1852
BtsI GCAGTG_nn'
Cuts at: 0 4020 8726 9608 10254
Size: 4020 4706 882 646
Fragments arranged by size:
4706 4020 882 646
ClaI AT'CG_AT
Cuts at: 0 3514 10254
Size: 3514 6740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 8543 8578 10254
Size: 8543 35 1676
Fragments arranged by size:
8543 1676 35
DraI TTT'AAA
Cuts at: 0 186 822 1587 2471 2912 6048 6390 7230
Size: 186 636 765 884 441 3136 342 840
Cuts at: 7230 9975 10254
Size: 2745 279
Fragments arranged by size:
3136 2745 884 840 765 636 441 342
279 186
DraIII CAC_nnn'GTG
Cuts at: 0 5842 7415 10254
Size: 5842 1573 2839
Fragments arranged by size:
5842 2839 1573
DrdI GACnn_nn'nnGTC
Cuts at: 0 80 344 2990 9750 10254
Size: 80 264 2646 6760 504
Fragments arranged by size:
6760 2646 504 264 80
EaeI y'GGCC_r
Cuts at: 0 1687 4107 8147 9701 10254
Size: 1687 2420 4040 1554 553
Fragments arranged by size:
4040 2420 1687 1554 553
EarI CTCTTCn'nnn_
Cuts at: 0 1403 2186 3070 3409 5454 6810 8762 9798
Size: 1403 783 884 339 2045 1356 1952 1036
Cuts at: 9798 10254
Size: 456
Fragments arranged by size:
2045 1952 1403 1356 1036 884 783 456
339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 739 929 1088 1307 1460 1619 1976
Size: 92 647 190 159 219 153 159 357
Cuts at: 1976 2657 3009 3713 5846 6089 6410 6914 7435
Size: 681 352 704 2133 243 321 504 521
Cuts at: 7435 7712 7805 9153 9358 9440 9581 10254
Size: 277 93 1348 205 82 141 673
Fragments arranged by size:
2133 1348 704 681 673 647 521 504
357 352 321 277 243 219 205 190
159 159 153 141 93 92 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 9328 9629 10254
Size: 9328 301 625
Fragments arranged by size:
9328 625 301
EcoRI G'AATT_C
Cuts at: 0 813 1329 1707 4785 9951 10254
Size: 813 516 378 3078 5166 303
Fragments arranged by size:
5166 3078 813 516 378 303
EcoRV GAT'ATC
Cuts at: 0 5284 10254
Size: 5284 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1333 1900 3797 6045 6532 10254
Size: 1333 567 1897 2248 487 3722
Fragments arranged by size:
3722 2248 1897 1333 567 487
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1777 1809 1813 1845 2110 2142
Size: 1132 32 613 32 4 32 265 32
Cuts at: 2142 2245 2277 2320 2352 2886 2899 2918 2931
Size: 103 32 43 32 534 13 19 13
Cuts at: 2931 3813 3845 5626 5658 7020 7052 8226 8258
Size: 882 32 1781 32 1362 32 1174 32
Cuts at: 8258 8485 8517 9639 9660 9671 9692 10254
Size: 227 32 1122 21 11 21 562
Fragments arranged by size:
1781 1362 1174 1132 1122 882 613 562
534 265 227 103 43 32 32 32
32 32 32 32 32 32 32 32
21 21 19 13 13 11 4
HincII GTy'rAC
Cuts at: 0 4226 5101 8999 10254
Size: 4226 875 3898 1255
Fragments arranged by size:
4226 3898 1255 875
HindIII A'AGCT_T
Cuts at: 0 5890 6695 7837 9535 9640 10254
Size: 5890 805 1142 1698 105 614
Fragments arranged by size:
5890 1698 1142 805 614 105
HpaI GTT'AAC
Cuts at: 0 4226 8999 10254
Size: 4226 4773 1255
Fragments arranged by size:
4773 4226 1255
MluI A'CGCG_T
Cuts at: 0 7503 10254
Size: 7503 2751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8816 10254
Size: 8816 1438
MscI TGG'CCA
Cuts at: 0 1689 4109 8149 9703 10254
Size: 1689 2420 4040 1554 551
Fragments arranged by size:
4040 2420 1689 1554 551
MslI CAynn'nnrTG
Cuts at: 0 2291 4052 4922 5258 7085 8902 9557 10254
Size: 2291 1761 870 336 1827 1817 655 697
Fragments arranged by size:
2291 1827 1817 1761 870 697 655 336
MspA1I CmG'CkG
Cuts at: 0 219 267 4759 7399 7617 8044 10254
Size: 219 48 4492 2640 218 427 2210
Fragments arranged by size:
4492 2640 2210 427 219 218 48
NcoI C'CATG_G
Cuts at: 0 2441 2777 8852 10254
Size: 2441 336 6075 1402
Fragments arranged by size:
6075 2441 1402 336
NdeI CA'TA_TG
Cuts at: 0 1568 4090 7882 9350 10254
Size: 1568 2522 3792 1468 904
Fragments arranged by size:
3792 2522 1568 1468 904
NheI G'CTAG_C
Cuts at: 0 4579 10254
Size: 4579 5675
NsiI A_TGCA'T
Cuts at: 0 5397 6968 9545 10254
Size: 5397 1571 2577 709
Fragments arranged by size:
5397 2577 1571 709
NspI r_CATG'y
Cuts at: 0 451 2027 2213 4196 4739 5235 6767 9556
Size: 451 1576 186 1983 543 496 1532 2789
Cuts at: 9556 10254
Size: 698
Fragments arranged by size:
2789 1983 1576 1532 698 543 496 451
186
PciI A'CATG_T
Cuts at: 0 447 2023 6763 10254
Size: 447 1576 4740 3491
Fragments arranged by size:
4740 3491 1576 447
PflMI CCAn_nnn'nTGG
Cuts at: 0 3517 8145 10254
Size: 3517 4628 2109
Fragments arranged by size:
4628 3517 2109
PmlI CAC'GTG
Cuts at: 0 8662 10254
Size: 8662 1592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1992 2024 7574 7606 10254
Size: 1992 32 5550 32 2648
Fragments arranged by size:
5550 2648 1992 32 32
PsiI TTA'TAA
Cuts at: 0 212 1799 2063 2483 7862 10254
Size: 212 1587 264 420 5379 2392
Fragments arranged by size:
5379 2392 1587 420 264 212
PstI C_TGCA'G
Cuts at: 0 141 2138 2208 3759 5124 6379 10254
Size: 141 1997 70 1551 1365 1255 3875
Fragments arranged by size:
3875 1997 1551 1365 1255 141 70
PvuII CAG'CTG
Cuts at: 0 219 4759 7399 7617 8044 10254
Size: 219 4540 2640 218 427 2210
Fragments arranged by size:
4540 2640 2210 427 219 218
SapI GCTCTTCn'nnn_
Cuts at: 0 1403 3070 5454 8762 9798 10254
Size: 1403 1667 2384 3308 1036 456
Fragments arranged by size:
3308 2384 1667 1403 1036 456
SbfI CC_TGCA'GG
Cuts at: 0 2138 10254
Size: 2138 8116
ScaI AGT'ACT
Cuts at: 0 5166 8346 10254
Size: 5166 3180 1908
Fragments arranged by size:
5166 3180 1908
SexAI A'CCwGG_T
Cuts at: 0 7053 10254
Size: 7053 3201
SfcI C'TryA_G
Cuts at: 0 137 1712 2092 2134 2204 3755 5120 5168
Size: 137 1575 380 42 70 1551 1365 48
Cuts at: 5168 6375 7193 7869 10254
Size: 1207 818 676 2385
Fragments arranged by size:
2385 1575 1551 1365 1207 818 676 380
137 70 48 42
SmlI C'TyrA_G
Cuts at: 0 4184 4845 8749 9819 10254
Size: 4184 661 3904 1070 435
Fragments arranged by size:
4184 3904 1070 661 435
SnaBI TAC'GTA
Cuts at: 0 6100 7625 10254
Size: 6100 1525 2629
Fragments arranged by size:
6100 2629 1525
SpeI A'CTAG_T
Cuts at: 0 3118 4506 4657 5353 8122 10254
Size: 3118 1388 151 696 2769 2132
Fragments arranged by size:
3118 2769 2132 1388 696 151
SphI G_CATG'C
Cuts at: 0 9556 10254
Size: 9556 698
SspI AAT'ATT
Cuts at: 0 239 1266 2436 3738 3891 4305 5090 7275
Size: 239 1027 1170 1302 153 414 785 2185
Cuts at: 7275 9504 10254
Size: 2229 750
Fragments arranged by size:
2229 2185 1302 1170 1027 785 750 414
239 153
StuI AGG'CCT
Cuts at: 0 4714 9401 10254
Size: 4714 4687 853
Fragments arranged by size:
4714 4687 853
StyI C'CwwG_G
Cuts at: 0 1151 2324 2441 2777 3149 8852 9809 9901
Size: 1151 1173 117 336 372 5703 957 92
Cuts at: 9901 10254
Size: 353
Fragments arranged by size:
5703 1173 1151 957 372 353 336 117
92
SwaI ATTT'AAAT
Cuts at: 0 6390 10254
Size: 6390 3864
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 2231 8159 9945 10254
Size: 618 1613 5928 1786 309
Fragments arranged by size:
5928 1786 1613 618 309
TatI w'GTAC_w
Cuts at: 0 442 2220 3418 4703 4914 5164 6175 8293
Size: 442 1778 1198 1285 211 250 1011 2118
Cuts at: 8293 8344 9592 9859 10156 10254
Size: 51 1248 267 297 98
Fragments arranged by size:
2118 1778 1285 1248 1198 1011 442 297
267 250 211 98 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 545 577 2131 2163 5526 5558 6494 6526
Size: 545 32 1554 32 3363 32 936 32
Cuts at: 6526 10254
Size: 3728
Fragments arranged by size:
3728 3363 1554 936 545 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 2011 2356 5721 6292 7846 10254
Size: 2011 345 3365 571 1554 2408
Fragments arranged by size:
3365 2408 2011 1554 571 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5806 10254
Size: 5806 4448
XmnI GAAnn'nnTTC
Cuts at: 0 1938 3033 5034 10254
Size: 1938 1095 2001 5220
Fragments arranged by size:
5220 2001 1938 1095
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EarI Eco57MI
EcoNI EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NsiI NspI PciI PflMI PmlI PpiI PsiI PstI
PvuII SapI SbfI ScaI SexAI SfcI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI ApaI AscI AsiSI AvaI AvrII
BbeI BciVI BglI BglII BlpI BmgBI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BstBI BstEII BstXI EagI
EciI EcoICRI EcoO109I FseI FspI FspAI HaeII KasI
KpnI MfeI NaeI NarI NgoMIV NotI NruI PacI
PasI PfoI PmeI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SfiI SfoI
SgfI SgrAI SmaI SrfI Tth111I XhoI XmaI ZraI