Restriction map for
pF1KE0914
(Linear) (Six-base) MAPSORT of: KIEE0914.seq Check: 7632 from: 1 to: 771
KIEE0914.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AclI AA'CG_TT
Cuts at: 0 11 771
Size: 11 760
AlwNI CAG_nnn'CTG
Cuts at: 0 509 540 771
Size: 509 31 231
Fragments arranged by size:
509 231 31
ApoI r'AATT_y
Cuts at: 0 154 771
Size: 154 617
AvaI C'yCGr_G
Cuts at: 0 703 771
Size: 703 68
BanI G'GyrC_C
Cuts at: 0 129 771
Size: 129 642
BanII G_rGCy'C
Cuts at: 0 206 710 771
Size: 206 504 61
Fragments arranged by size:
504 206 61
BbeI G_GCGC'C
Cuts at: 0 133 771
Size: 133 638
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 771
Size: 573 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 504 621 771
Size: 316 188 117 150
Fragments arranged by size:
316 188 150 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 358 771
Size: 358 413
BsaHI Gr'CG_yC
Cuts at: 0 130 771
Size: 130 641
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 314 344 771
Size: 314 30 427
Fragments arranged by size:
427 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 335 663 739 771
Size: 94 3 238 328 76 32
Fragments arranged by size:
328 238 94 76 32 3
BseYI C'CCAG_C
Cuts at: 0 133 315 771
Size: 133 182 456
Fragments arranged by size:
456 182 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 771
Size: 76 695
BsiEI CG_ry'CG
Cuts at: 0 287 419 771
Size: 287 132 352
Fragments arranged by size:
352 287 132
BsiHKAI G_wGCw'C
Cuts at: 0 719 771
Size: 719 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 438 771
Size: 438 333
Bsp1286I G_dGCh'C
Cuts at: 0 206 710 719 771
Size: 206 504 9 52
Fragments arranged by size:
504 206 52 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 539 771
Size: 539 232
BsrBI CCG'CTC
Cuts at: 0 197 407 771
Size: 197 210 364
Fragments arranged by size:
364 210 197
BssHII G'CGCG_C
Cuts at: 0 463 771
Size: 463 308
BstEII G'GTnAC_C
Cuts at: 0 303 771
Size: 303 468
BstYI r'GATC_y
Cuts at: 0 537 771
Size: 537 234
BtgI C'CryG_G
Cuts at: 0 281 495 771
Size: 281 214 276
Fragments arranged by size:
281 276 214
EaeI y'GGCC_r
Cuts at: 0 284 416 498 771
Size: 284 132 82 273
Fragments arranged by size:
284 273 132 82
EagI C'GGCC_G
Cuts at: 0 284 416 771
Size: 284 132 355
Fragments arranged by size:
355 284 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 504 621 771
Size: 316 188 117 150
Fragments arranged by size:
316 188 150 117
EcoRV GAT'ATC
Cuts at: 0 144 771
Size: 144 627
HaeII r_GCGC'y
Cuts at: 0 133 548 644 771
Size: 133 415 96 127
Fragments arranged by size:
415 133 127 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 592 624 771
Size: 34 32 526 32 147
Fragments arranged by size:
526 147 34 32 32
KasI G'GCGC_C
Cuts at: 0 129 771
Size: 129 642
MspA1I CmG'CkG
Cuts at: 0 294 476 497 771
Size: 294 182 21 274
Fragments arranged by size:
294 274 182 21
NarI GG'CG_CC
Cuts at: 0 130 771
Size: 130 641
PfoI T'CCnGG_A
Cuts at: 0 389 771
Size: 389 382
PstI C_TGCA'G
Cuts at: 0 122 536 771
Size: 122 414 235
Fragments arranged by size:
414 235 122
PvuII CAG'CTG
Cuts at: 0 294 771
Size: 294 477
SacII CC_GC'GG
Cuts at: 0 498 771
Size: 498 273
SbfI CC_TGCA'GG
Cuts at: 0 536 771
Size: 536 235
SfcI C'TryA_G
Cuts at: 0 118 532 771
Size: 118 414 239
Fragments arranged by size:
414 239 118
SfoI GGC'GCC
Cuts at: 0 131 771
Size: 131 640
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 771
Size: 322 449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 385 417 771
Size: 314 32 39 32 354
Fragments arranged by size:
354 314 39 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 681 771
Size: 681 90
XmnI GAAnn'nnTTC
Cuts at: 0 154 771
Size: 154 617
Enzymes that do cut:
AclI AlwNI ApoI AvaI BanI BanII BbeI BbsI
BpmI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssHII BstEII BstYI
BtgI EaeI EagI Eco57MI EcoRV HaeII Hin4I KasI
MspA1I NarI PfoI PstI PvuII SacII SbfI SfcI
SfoI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI XbaI XcmI XhoI XmaI ZraI