Restriction map for
pF1KE0916
(Linear) (Six-base) MAPSORT of: KIEE0916.seq Check: 3627 from: 1 to: 861
KIEE0916.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 326 861
Size: 326 535
AfeI AGC'GCT
Cuts at: 0 385 861
Size: 385 476
AlwNI CAG_nnn'CTG
Cuts at: 0 525 861
Size: 525 336
ApaI G_GGCC'C
Cuts at: 0 490 861
Size: 490 371
AvaI C'yCGr_G
Cuts at: 0 514 633 717 861
Size: 514 119 84 144
Fragments arranged by size:
514 144 119 84
BanII G_rGCy'C
Cuts at: 0 473 490 861
Size: 473 17 371
Fragments arranged by size:
473 371 17
BclI T'GATC_A
Cuts at: 0 170 861
Size: 170 691
BlpI GC'TnA_GC
Cuts at: 0 184 861
Size: 184 677
Bme1580I G_kGCm'C
Cuts at: 0 490 861
Size: 490 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 484 787 843 861
Size: 136 348 303 56 18
Fragments arranged by size:
348 303 136 56 18
BsaAI yAC'GTr
Cuts at: 0 68 176 650 861
Size: 68 108 474 211
Fragments arranged by size:
474 211 108 68
BsaBI GATnn'nnATC
Cuts at: 0 133 861
Size: 133 728
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 861
Size: 103 30 728
Fragments arranged by size:
728 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 255 481 861
Size: 255 226 380
Fragments arranged by size:
380 255 226
BseYI C'CCAG_C
Cuts at: 0 374 603 622 861
Size: 374 229 19 239
Fragments arranged by size:
374 239 229 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 188 477 861
Size: 48 140 289 384
Fragments arranged by size:
384 289 140 48
BsiEI CG_ry'CG
Cuts at: 0 351 861
Size: 351 510
BsiHKAI G_wGCw'C
Cuts at: 0 292 861
Size: 292 569
Bsp1286I G_dGCh'C
Cuts at: 0 292 473 490 861
Size: 292 181 17 371
Fragments arranged by size:
371 292 181 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 312 425 443 861
Size: 312 113 18 418
Fragments arranged by size:
418 312 113 18
BsrFI r'CCGG_y
Cuts at: 0 221 861
Size: 221 640
BssHII G'CGCG_C
Cuts at: 0 549 861
Size: 549 312
BssSI C'ACGA_G
Cuts at: 0 424 861
Size: 424 437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 780 861
Size: 780 81
BtsI GCAGTG_nn'
Cuts at: 0 753 861
Size: 753 108
EaeI y'GGCC_r
Cuts at: 0 356 708 861
Size: 356 352 153
Fragments arranged by size:
356 352 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 861
Size: 252 609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 484 787 843 861
Size: 136 348 303 56 18
Fragments arranged by size:
348 303 136 56 18
EcoO109I rG'GnC_Cy
Cuts at: 0 486 487 510 722 773 861
Size: 486 1 23 212 51 88
Fragments arranged by size:
486 212 88 51 23 1
HaeII r_GCGC'y
Cuts at: 0 387 647 861
Size: 387 260 214
Fragments arranged by size:
387 260 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 842 861
Size: 241 32 569 19
Fragments arranged by size:
569 241 32 19
HincII GTy'rAC
Cuts at: 0 21 861
Size: 21 840
MslI CAynn'nnrTG
Cuts at: 0 139 142 861
Size: 139 3 719
Fragments arranged by size:
719 139 3
MspA1I CmG'CkG
Cuts at: 0 378 525 662 861
Size: 378 147 137 199
Fragments arranged by size:
378 199 147 137
NaeI GCC'GGC
Cuts at: 0 223 861
Size: 223 638
NgoMIV G'CCGG_C
Cuts at: 0 221 861
Size: 221 640
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 861
Size: 99 762
PmlI CAC'GTG
Cuts at: 0 68 176 650 861
Size: 68 108 474 211
Fragments arranged by size:
474 211 108 68
PpuMI rG'GwC_Cy
Cuts at: 0 510 722 773 861
Size: 510 212 51 88
Fragments arranged by size:
510 212 88 51
PspOMI G'GGCC_C
Cuts at: 0 486 861
Size: 486 375
PstI C_TGCA'G
Cuts at: 0 422 861
Size: 422 439
PvuII CAG'CTG
Cuts at: 0 525 861
Size: 525 336
SanDI GG'GwC_CC
Cuts at: 0 510 773 861
Size: 510 263 88
Fragments arranged by size:
510 263 88
SfcI C'TryA_G
Cuts at: 0 418 693 861
Size: 418 275 168
Fragments arranged by size:
418 275 168
SmaI CCC'GGG
Cuts at: 0 719 861
Size: 719 142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 745 861
Size: 351 394 116
Fragments arranged by size:
394 351 116
XmaI C'CCGG_G
Cuts at: 0 717 861
Size: 717 144
Enzymes that do cut:
AccI AfeI AlwNI ApaI AvaI BanII BclI BlpI
Bme1580I BpmI BsaAI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BtgZI
BtsI EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII
MslI MspA1I NaeI NgoMIV PflMI PmlI PpuMI PspOMI
PstI PvuII SanDI SfcI SmaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmnI ZraI