Restriction map for
pF1KE0921
(Linear) (Six-base) MAPSORT of: KIEE0921.seq Check: 9444 from: 1 to: 846
KIEE0921.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
Acc65I G'GTAC_C
Cuts at: 0 34 846
Size: 34 812
AflIII A'CryG_T
Cuts at: 0 208 846
Size: 208 638
AgeI A'CCGG_T
Cuts at: 0 31 846
Size: 31 815
ApaI G_GGCC'C
Cuts at: 0 232 846
Size: 232 614
ApoI r'AATT_y
Cuts at: 0 657 846
Size: 657 189
AseI AT'TA_AT
Cuts at: 0 509 846
Size: 509 337
BamHI G'GATC_C
Cuts at: 0 238 846
Size: 238 608
BanI G'GyrC_C
Cuts at: 0 34 63 846
Size: 34 29 783
Fragments arranged by size:
783 34 29
BanII G_rGCy'C
Cuts at: 0 232 359 846
Size: 232 127 487
Fragments arranged by size:
487 232 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 846
Size: 296 550
BfrBI ATG'CAT
Cuts at: 0 447 846
Size: 447 399
Bme1580I G_kGCm'C
Cuts at: 0 232 391 846
Size: 232 159 455
Fragments arranged by size:
455 232 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 846
Size: 540 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 846
Size: 694 152
BsaWI w'CCGG_w
Cuts at: 0 31 846
Size: 31 815
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 707 846
Size: 707 139
BsiHKAI G_wGCw'C
Cuts at: 0 359 846
Size: 359 487
Bsp1286I G_dGCh'C
Cuts at: 0 232 359 391 846
Size: 232 127 32 455
Fragments arranged by size:
455 232 127 32
BspHI T'CATG_A
Cuts at: 0 729 846
Size: 729 117
BsrDI GCAATG_nn'
Cuts at: 0 785 846
Size: 785 61
BsrFI r'CCGG_y
Cuts at: 0 31 846
Size: 31 815
BstEII G'GTnAC_C
Cuts at: 0 601 846
Size: 601 245
BstYI r'GATC_y
Cuts at: 0 238 846
Size: 238 608
DrdI GACnn_nn'nnGTC
Cuts at: 0 769 846
Size: 769 77
EaeI y'GGCC_r
Cuts at: 0 171 846
Size: 171 675
EarI CTCTTCn'nnn_
Cuts at: 0 347 846
Size: 347 499
EcoICRI GAG'CTC
Cuts at: 0 357 846
Size: 357 489
EcoO109I rG'GnC_Cy
Cuts at: 0 228 229 846
Size: 228 1 617
Fragments arranged by size:
617 228 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 846
Size: 159 32 655
Fragments arranged by size:
655 159 32
HincII GTy'rAC
Cuts at: 0 528 846
Size: 528 318
KpnI G_GTAC'C
Cuts at: 0 38 846
Size: 38 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 241 846
Size: 116 125 605
Fragments arranged by size:
605 125 116
MscI TGG'CCA
Cuts at: 0 173 846
Size: 173 673
MspA1I CmG'CkG
Cuts at: 0 630 846
Size: 630 216
NsiI A_TGCA'T
Cuts at: 0 449 846
Size: 449 397
NspI r_CATG'y
Cuts at: 0 212 846
Size: 212 634
PciI A'CATG_T
Cuts at: 0 208 846
Size: 208 638
PspOMI G'GGCC_C
Cuts at: 0 228 846
Size: 228 618
PvuII CAG'CTG
Cuts at: 0 630 846
Size: 630 216
SacI G_AGCT'C
Cuts at: 0 359 846
Size: 359 487
SapI GCTCTTCn'nnn_
Cuts at: 0 347 846
Size: 347 499
ScaI AGT'ACT
Cuts at: 0 281 846
Size: 281 565
SfcI C'TryA_G
Cuts at: 0 17 472 500 846
Size: 17 455 28 346
Fragments arranged by size:
455 346 28 17
SmlI C'TyrA_G
Cuts at: 0 673 846
Size: 673 173
StuI AGG'CCT
Cuts at: 0 372 846
Size: 372 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 522 846
Size: 522 324
TatI w'GTAC_w
Cuts at: 0 279 846
Size: 279 567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 846
Size: 198 32 616
Fragments arranged by size:
616 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 249 846
Size: 99 150 597
Fragments arranged by size:
597 150 99
Enzymes that do cut:
Acc65I AflIII AgeI ApaI ApoI AseI BamHI BanI
BanII BbsI BfrBI Bme1580I BmrI BpuEI BsaWI BseRI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstEII BstYI DrdI
EaeI EarI EcoICRI EcoO109I Hin4I HincII KpnI MmeI
MscI MspA1I NsiI NspI PciI PspOMI PvuII SacI
SapI ScaI SfcI SmlI StuI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII EagI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI