Restriction map for
pF1KE0922
(Linear) (Six-base) MAPSORT of: KIEE0922.seq Check: 99 from: 1 to: 2019
KIEE0922.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 999 2019
Size: 285 714 1020
Fragments arranged by size:
1020 714 285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 973 1078 1270 1473 2005 2019
Size: 676 297 105 192 203 532 14
Fragments arranged by size:
676 532 297 203 192 105 14
AfeI AGC'GCT
Cuts at: 0 1112 2019
Size: 1112 907
AflIII A'CryG_T
Cuts at: 0 1633 2019
Size: 1633 386
AhdI GACnn_n'nnGTC
Cuts at: 0 1251 2019
Size: 1251 768
ApoI r'AATT_y
Cuts at: 0 1918 2019
Size: 1918 101
AvaI C'yCGr_G
Cuts at: 0 315 806 1046 1135 2019
Size: 315 491 240 89 884
Fragments arranged by size:
884 491 315 240 89
BamHI G'GATC_C
Cuts at: 0 1496 2019
Size: 1496 523
BanI G'GyrC_C
Cuts at: 0 19 2019
Size: 19 2000
BanII G_rGCy'C
Cuts at: 0 37 1295 1471 2019
Size: 37 1258 176 548
Fragments arranged by size:
1258 548 176 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 664 949 1154 1677 1864 2019
Size: 664 285 205 523 187 155
Fragments arranged by size:
664 523 285 205 187 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 613 647 2019
Size: 613 34 1372
Fragments arranged by size:
1372 613 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 754 1890 2019
Size: 754 1136 129
Fragments arranged by size:
1136 754 129
BclI T'GATC_A
Cuts at: 0 911 2019
Size: 911 1108
BfrBI ATG'CAT
Cuts at: 0 1765 2019
Size: 1765 254
BglI GCCn_nnn'nGGC
Cuts at: 0 1573 1996 2019
Size: 1573 423 23
Fragments arranged by size:
1573 423 23
BglII A'GATC_T
Cuts at: 0 350 872 1259 1583 2019
Size: 350 522 387 324 436
Fragments arranged by size:
522 436 387 350 324
BmgBI CAC'GTC
Cuts at: 0 1719 2019
Size: 1719 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 2019
Size: 585 1434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 469 1286 1474 2019
Size: 445 24 817 188 545
Fragments arranged by size:
817 545 445 188 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 840 1262 2019
Size: 840 422 757
Fragments arranged by size:
840 757 422
BsaBI GATnn'nnATC
Cuts at: 0 650 826 2019
Size: 650 176 1193
Fragments arranged by size:
1193 650 176
BsaWI w'CCGG_w
Cuts at: 0 746 878 1676 1910 1972 2019
Size: 746 132 798 234 62 47
Fragments arranged by size:
798 746 234 132 62 47
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 2019
Size: 19 30 1970
Fragments arranged by size:
1970 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 1543 1550 2019
Size: 514 1029 7 469
Fragments arranged by size:
1029 514 469 7
BseYI C'CCAG_C
Cuts at: 0 1074 2019
Size: 1074 945
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 2019
Size: 1058 961
BsiHKAI G_wGCw'C
Cuts at: 0 754 1471 1727 2019
Size: 754 717 256 292
Fragments arranged by size:
754 717 292 256
Bsp1286I G_dGCh'C
Cuts at: 0 37 754 1295 1471 1727 2019
Size: 37 717 541 176 256 292
Fragments arranged by size:
717 541 292 256 176 37
BspEI T'CCGG_A
Cuts at: 0 746 878 1676 1910 1972 2019
Size: 746 132 798 234 62 47
Fragments arranged by size:
798 746 234 132 62 47
BspHI T'CATG_A
Cuts at: 0 1295 1640 2019
Size: 1295 345 379
Fragments arranged by size:
1295 379 345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 999 1907 2019
Size: 285 714 908 112
Fragments arranged by size:
908 714 285 112
BsrBI CCG'CTC
Cuts at: 0 417 2019
Size: 417 1602
BsrDI GCAATG_nn'
Cuts at: 0 209 1188 2019
Size: 209 979 831
Fragments arranged by size:
979 831 209
BsrFI r'CCGG_y
Cuts at: 0 1305 1351 2019
Size: 1305 46 668
Fragments arranged by size:
1305 668 46
BsrGI T'GTAC_A
Cuts at: 0 86 2019
Size: 86 1933
BstAPI GCAn_nnn'nTGC
Cuts at: 0 19 2019
Size: 19 2000
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1875 2019
Size: 1875 144
BstYI r'GATC_y
Cuts at: 0 350 872 1199 1259 1496 1583 2019
Size: 350 522 327 60 237 87 436
Fragments arranged by size:
522 436 350 327 237 87 60
BtgI C'CryG_G
Cuts at: 0 1340 1514 2019
Size: 1340 174 505
Fragments arranged by size:
1340 505 174
BtsI GCAGTG_nn'
Cuts at: 0 89 117 1046 1893 2019
Size: 89 28 929 847 126
Fragments arranged by size:
929 847 126 89 28
ClaI AT'CG_AT
Cuts at: 0 1160 2019
Size: 1160 859
DraI TTT'AAA
Cuts at: 0 221 2019
Size: 221 1798
DraIII CAC_nnn'GTG
Cuts at: 0 19 1609 2019
Size: 19 1590 410
Fragments arranged by size:
1590 410 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 1215 2019
Size: 1215 804
EaeI y'GGCC_r
Cuts at: 0 2 453 728 984 1227 1385 1482 1511
Size: 2 451 275 256 243 158 97 29
Cuts at: 1511 1772 2019
Size: 261 247
Fragments arranged by size:
451 275 261 256 247 243 158 97
29 2
EarI CTCTTCn'nnn_
Cuts at: 0 784 1810 2019
Size: 784 1026 209
Fragments arranged by size:
1026 784 209
EcoICRI GAG'CTC
Cuts at: 0 1469 2019
Size: 1469 550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 469 676 973 1078 1270 1286 1473
Size: 445 24 207 297 105 192 16 187
Cuts at: 1473 1474 2005 2019
Size: 1 531 14
Fragments arranged by size:
531 445 297 207 192 187 105 24
16 14 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 484 553 1549 2019
Size: 484 69 996 470
Fragments arranged by size:
996 484 470 69
EcoO109I rG'GnC_Cy
Cuts at: 0 204 420 522 546 795 2019
Size: 204 216 102 24 249 1224
Fragments arranged by size:
1224 249 216 204 102 24
EcoRI G'AATT_C
Cuts at: 0 1918 2019
Size: 1918 101
EcoRV GAT'ATC
Cuts at: 0 1164 2019
Size: 1164 855
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1239 1821 2019
Size: 1239 582 198
Fragments arranged by size:
1239 582 198
HaeII r_GCGC'y
Cuts at: 0 1114 2019
Size: 1114 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 1237 1269 2019
Size: 298 32 907 32 750
Fragments arranged by size:
907 750 298 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 2019
Size: 830 1189
MscI TGG'CCA
Cuts at: 0 4 455 986 1387 1484 1513 1774 2019
Size: 4 451 531 401 97 29 261 245
Fragments arranged by size:
531 451 401 261 245 97 29 4
MslI CAynn'nnrTG
Cuts at: 0 94 1316 1702 2019
Size: 94 1222 386 317
Fragments arranged by size:
1222 386 317 94
MspA1I CmG'CkG
Cuts at: 0 387 1191 2019
Size: 387 804 828
Fragments arranged by size:
828 804 387
NaeI GCC'GGC
Cuts at: 0 1353 2019
Size: 1353 666
NcoI C'CATG_G
Cuts at: 0 1340 2019
Size: 1340 679
NgoMIV G'CCGG_C
Cuts at: 0 1351 2019
Size: 1351 668
NsiI A_TGCA'T
Cuts at: 0 1767 2019
Size: 1767 252
NspI r_CATG'y
Cuts at: 0 858 2019
Size: 858 1161
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 2019
Size: 390 1629
PfoI T'CCnGG_A
Cuts at: 0 361 542 995 1688 2019
Size: 361 181 453 693 331
Fragments arranged by size:
693 453 361 331 181
PpuMI rG'GwC_Cy
Cuts at: 0 204 420 522 546 2019
Size: 204 216 102 24 1473
Fragments arranged by size:
1473 216 204 102 24
PvuII CAG'CTG
Cuts at: 0 387 2019
Size: 387 1632
SacI G_AGCT'C
Cuts at: 0 1471 2019
Size: 1471 548
SapI GCTCTTCn'nnn_
Cuts at: 0 784 2019
Size: 784 1235
SexAI A'CCwGG_T
Cuts at: 0 524 2019
Size: 524 1495
SfcI C'TryA_G
Cuts at: 0 903 2019
Size: 903 1116
SspI AAT'ATT
Cuts at: 0 763 2019
Size: 763 1256
StuI AGG'CCT
Cuts at: 0 1426 1990 2019
Size: 1426 564 29
Fragments arranged by size:
1426 564 29
StyI C'CwwG_G
Cuts at: 0 1340 1625 2019
Size: 1340 285 394
Fragments arranged by size:
1340 394 285
TatI w'GTAC_w
Cuts at: 0 86 935 2019
Size: 86 849 1084
Fragments arranged by size:
1084 849 86
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1716 2019
Size: 1716 303
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI ApoI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BpmI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI
BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PflMI PfoI PpuMI PvuII SacI SapI SexAI
SfcI SspI StuI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BlpI Bme1580I BmtI BplI Bpu10I
BpuEI BsaAI BsaHI BsiEI BsiWI BsmI BsmBI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI EagI
EciI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI