(Linear) (Six-base) MAPSORT of: KIEE0925.seq Check: 2082 from: 1 to: 984 KIEE0925.orf With 182 enzymes: SgfI * April 24, 2009 13:19 .. AlwNI CAG_nnn'CTG Cuts at: 0 199 984 Size: 199 785 ApoI r'AATT_y Cuts at: 0 123 346 448 644 984 Size: 123 223 102 196 340 Fragments arranged by size: 340 223 196 123 102 AseI AT'TA_AT Cuts at: 0 251 984 Size: 251 733 AvaI C'yCGr_G Cuts at: 0 43 984 Size: 43 941 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 613 646 984 Size: 613 33 338 Fragments arranged by size: 613 338 33 BanI G'GyrC_C Cuts at: 0 23 984 Size: 23 961 BanII G_rGCy'C Cuts at: 0 62 90 984 Size: 62 28 894 Fragments arranged by size: 894 62 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 42 466 984 Size: 42 424 518 Fragments arranged by size: 518 424 42 BciVI GTATCCnnnnn_n' Cuts at: 0 614 735 984 Size: 614 121 249 Fragments arranged by size: 614 249 121 BlpI GC'TnA_GC Cuts at: 0 239 984 Size: 239 745 Bpu10I CC'TnA_GC Cuts at: 0 971 984 Size: 971 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 984 Size: 254 730 BsaBI GATnn'nnATC Cuts at: 0 746 984 Size: 746 238 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 77 107 984 Size: 77 30 877 Fragments arranged by size: 877 77 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 98 984 Size: 98 886 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 614 984 Size: 614 370 BsiHKAI G_wGCw'C Cuts at: 0 62 984 Size: 62 922 Bsp1286I G_dGCh'C Cuts at: 0 62 90 984 Size: 62 28 894 Fragments arranged by size: 894 62 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 234 984 Size: 234 750 BsrDI GCAATG_nn' Cuts at: 0 15 984 Size: 15 969 BtgI C'CryG_G Cuts at: 0 737 984 Size: 737 247 DraI TTT'AAA Cuts at: 0 342 984 Size: 342 642 DrdI GACnn_nn'nnGTC Cuts at: 0 46 984 Size: 46 938 EaeI y'GGCC_r Cuts at: 0 662 984 Size: 662 322 EcoICRI GAG'CTC Cuts at: 0 60 984 Size: 60 924 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 984 Size: 316 32 636 Fragments arranged by size: 636 316 32 MscI TGG'CCA Cuts at: 0 664 984 Size: 664 320 MslI CAynn'nnrTG Cuts at: 0 308 984 Size: 308 676 NcoI C'CATG_G Cuts at: 0 737 984 Size: 737 247 NspI r_CATG'y Cuts at: 0 572 984 Size: 572 412 PflMI CCAn_nnn'nTGG Cuts at: 0 148 984 Size: 148 836 SacI G_AGCT'C Cuts at: 0 62 984 Size: 62 922 SmaI CCC'GGG Cuts at: 0 45 984 Size: 45 939 SmlI C'TyrA_G Cuts at: 0 269 984 Size: 269 715 SpeI A'CTAG_T Cuts at: 0 930 984 Size: 930 54 SphI G_CATG'C Cuts at: 0 572 984 Size: 572 412 StuI AGG'CCT Cuts at: 0 603 984 Size: 603 381 StyI C'CwwG_G Cuts at: 0 737 984 Size: 737 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 567 984 Size: 567 417 TatI w'GTAC_w Cuts at: 0 414 629 819 984 Size: 414 215 190 165 Fragments arranged by size: 414 215 190 165 XmaI C'CCGG_G Cuts at: 0 43 984 Size: 43 941 Enzymes that do cut: AlwNI ApoI AseI AvaI BaeI BanI BanII BbsI BciVI BlpI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BtgI DraI DrdI EaeI EcoICRI Hin4I MscI MslI NcoI NspI PflMI SacI SmaI SmlI SpeI SphI StuI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI