(Linear) (Six-base) MAPSORT of: KIEE0930.seq  Check: 1035  from: 1  to: 1155



KIEE0930.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     474    1029    1155 
   Size:        474     555     126

  Fragments arranged by size: 

                 555     474     126
 
ApaI G_GGCC'C

Cuts at:      0     525     610    1155 
   Size:        525      85     545

  Fragments arranged by size: 

                 545     525      85
 
ApoI r'AATT_y

Cuts at:      0     145     933    1155 
   Size:        145     788     222

  Fragments arranged by size: 

                 788     222     145
 
AvaI C'yCGr_G

Cuts at:      0    1082    1155 
   Size:       1082      73
 
BanI G'GyrC_C

Cuts at:      0     837     952    1155 
   Size:        837     115     203

  Fragments arranged by size: 

                 837     203     115
 
BanII G_rGCy'C

Cuts at:      0     525     610    1155 
   Size:        525      85     545

  Fragments arranged by size: 

                 545     525      85
 
BclI T'GATC_A

Cuts at:      0     342    1155 
   Size:        342     813
 
Bme1580I G_kGCm'C

Cuts at:      0     525     610    1155 
   Size:        525      85     545

  Fragments arranged by size: 

                 545     525      85
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     960    1028    1155 
   Size:        960      68     127

  Fragments arranged by size: 

                 960     127      68
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     675     808     829    1155 
   Size:        496     179     133      21     326

  Fragments arranged by size: 

                 496     326     179     133      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     458    1011    1155 
   Size:        458     553     144

  Fragments arranged by size: 

                 553     458     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231    1155 
   Size:        231     924
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1084    1155 
   Size:       1084      71
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1134    1147    1155 
   Size:       1134      13       8

  Fragments arranged by size: 

                1134      13       8
 
BseYI C'CCAG_C

Cuts at:      0     113     524    1155 
   Size:        113     411     631

  Fragments arranged by size: 

                 631     411     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53    1155 
   Size:         53    1102
 
Bsp1286I G_dGCh'C

Cuts at:      0     525     610    1155 
   Size:        525      85     545

  Fragments arranged by size: 

                 545     525      85
 
BsrDI GCAATG_nn'

Cuts at:      0      83     401    1155 
   Size:         83     318     754

  Fragments arranged by size: 

                 754     318      83
 
BsrFI r'CCGG_y

Cuts at:      0     612    1155 
   Size:        612     543
 
BstYI r'GATC_y

Cuts at:      0     737     813    1155 
   Size:        737      76     342

  Fragments arranged by size: 

                 737     342      76
 
BtsI GCAGTG_nn'

Cuts at:      0    1062    1068    1155 
   Size:       1062       6      87

  Fragments arranged by size: 

                1062      87       6
 
DraI TTT'AAA

Cuts at:      0     380    1155 
   Size:        380     775
 
EarI CTCTTCn'nnn_

Cuts at:      0     357    1155 
   Size:        357     798
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     510    1155 
   Size:        510     645
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     675     808     829    1155 
   Size:        496     179     133      21     326

  Fragments arranged by size: 

                 496     326     179     133      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117     606     880    1155 
   Size:        117     489     274     275

  Fragments arranged by size: 

                 489     275     274     117
 
EcoRV GAT'ATC

Cuts at:      0     870    1155 
   Size:        870     285
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      39      71     277     309    1129    1155 
   Size:         39      32     206      32     820      26

  Fragments arranged by size: 

                 820     206      39      32      32      26
 
MspA1I CmG'CkG

Cuts at:      0     528     636    1155 
   Size:        528     108     519

  Fragments arranged by size: 

                 528     519     108
 
NaeI GCC'GGC

Cuts at:      0     614    1155 
   Size:        614     541
 
NgoMIV G'CCGG_C

Cuts at:      0     612    1155 
   Size:        612     543
 
PpuMI rG'GwC_Cy

Cuts at:      0     117     880    1155 
   Size:        117     763     275

  Fragments arranged by size: 

                 763     275     117
 
PsiI TTA'TAA

Cuts at:      0       7    1155 
   Size:          7    1148
 
PspOMI G'GGCC_C

Cuts at:      0     521     606    1155 
   Size:        521      85     549

  Fragments arranged by size: 

                 549     521      85
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     527     559    1155 
   Size:        527      32     596

  Fragments arranged by size: 

                 596     527      32
 
PvuII CAG'CTG

Cuts at:      0     528     636    1155 
   Size:        528     108     519

  Fragments arranged by size: 

                 528     519     108
 
SanDI GG'GwC_CC

Cuts at:      0     117    1155 
   Size:        117    1038
 
ScaI AGT'ACT

Cuts at:      0     758    1155 
   Size:        758     397
 
SexAI A'CCwGG_T

Cuts at:      0     566    1155 
   Size:        566     589
 
SfcI C'TryA_G

Cuts at:      0     544    1155 
   Size:        544     611
 
SmlI C'TyrA_G

Cuts at:      0     210    1155 
   Size:        210     945
 
StyI C'CwwG_G

Cuts at:      0     981    1155 
   Size:        981     174
 
TatI w'GTAC_w

Cuts at:      0     756    1155 
   Size:        756     399
 

 Enzymes that do cut:

    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BclI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI     BsgI
 Bsp1286I    BsrDI    BsrFI    BstYI     BtsI     DraI     EarI     EciI
  Eco57MI EcoO109I    EcoRV    Hin4I   MspA1I     NaeI   NgoMIV    PpuMI
     PsiI   PspOMI     PsrI    PvuII    SanDI     ScaI    SexAI     SfcI
     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI    PspXI     PstI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI