Restriction map for
pF1KE0940
(Linear) (Six-base) MAPSORT of: KIEE0940.seq Check: 8203 from: 1 to: 1377
KIEE0940.orf
With 182 enzymes: SgfI *
August 9, 2016 17:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1135 1377
Size: 904 231 242
Fragments arranged by size:
904 242 231
AflIII A'CryG_T
Cuts at: 0 76 676 1179 1377
Size: 76 600 503 198
Fragments arranged by size:
600 503 198 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 800 1377
Size: 137 663 577
Fragments arranged by size:
663 577 137
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1377
Size: 309 1068
ApoI r'AATT_y
Cuts at: 0 32 872 1041 1160 1377
Size: 32 840 169 119 217
Fragments arranged by size:
840 217 169 119 32
AvrII C'CTAG_G
Cuts at: 0 216 1377
Size: 216 1161
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 548 581 1377
Size: 548 33 796
Fragments arranged by size:
796 548 33
BamHI G'GATC_C
Cuts at: 0 56 1377
Size: 56 1321
BanII G_rGCy'C
Cuts at: 0 857 1346 1377
Size: 857 489 31
Fragments arranged by size:
857 489 31
BbvCI CC'TCA_GC
Cuts at: 0 284 1377
Size: 284 1093
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 659 1377
Size: 625 34 718
Fragments arranged by size:
718 625 34
BclI T'GATC_A
Cuts at: 0 495 867 1377
Size: 495 372 510
Fragments arranged by size:
510 495 372
Bme1580I G_kGCm'C
Cuts at: 0 434 1377
Size: 434 943
BmgBI CAC'GTC
Cuts at: 0 435 1377
Size: 435 942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1377
Size: 1091 286
Bpu10I CC'TnA_GC
Cuts at: 0 284 388 1377
Size: 284 104 989
Fragments arranged by size:
989 284 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 1204 1377
Size: 400 804 173
Fragments arranged by size:
804 400 173
BsaAI yAC'GTr
Cuts at: 0 816 1377
Size: 816 561
BsaWI w'CCGG_w
Cuts at: 0 478 1377
Size: 478 899
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 1081 1084 1377
Size: 120 961 3 293
Fragments arranged by size:
961 293 120 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 685 1377
Size: 310 375 692
Fragments arranged by size:
692 375 310
BsiEI CG_ry'CG
Cuts at: 0 639 1377
Size: 639 738
BsiHKAI G_wGCw'C
Cuts at: 0 9 857 1346 1377
Size: 9 848 489 31
Fragments arranged by size:
848 489 31 9
BsmI GAATG_Cn'
Cuts at: 0 983 1377
Size: 983 394
Bsp1286I G_dGCh'C
Cuts at: 0 9 434 857 1346 1377
Size: 9 425 423 489 31
Fragments arranged by size:
489 425 423 31 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 452 1272 1377
Size: 452 820 105
Fragments arranged by size:
820 452 105
BsrFI r'CCGG_y
Cuts at: 0 1366 1377
Size: 1366 11
BsrGI T'GTAC_A
Cuts at: 0 362 1377
Size: 362 1015
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 1377
Size: 309 1068
BstYI r'GATC_y
Cuts at: 0 56 1377
Size: 56 1321
Bsu36I CC'TnA_GG
Cuts at: 0 996 1377
Size: 996 381
BtgI C'CryG_G
Cuts at: 0 689 1377
Size: 689 688
BtsI GCAGTG_nn'
Cuts at: 0 189 1377
Size: 189 1188
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 18 53 1377
Size: 18 35 1324
Fragments arranged by size:
1324 35 18
EarI CTCTTCn'nnn_
Cuts at: 0 689 1377
Size: 689 688
EcoICRI GAG'CTC
Cuts at: 0 855 1344 1377
Size: 855 489 33
Fragments arranged by size:
855 489 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1091 1135 1377
Size: 904 187 44 242
Fragments arranged by size:
904 242 187 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 138 1377
Size: 138 1239
EcoRV GAT'ATC
Cuts at: 0 1103 1377
Size: 1103 274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 114 1362 1377
Size: 114 1248 15
Fragments arranged by size:
1248 114 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 544 576 1377
Size: 34 32 478 32 801
Fragments arranged by size:
801 478 34 32 32
HindIII A'AGCT_T
Cuts at: 0 1147 1377
Size: 1147 230
MslI CAynn'nnrTG
Cuts at: 0 728 1202 1377
Size: 728 474 175
Fragments arranged by size:
728 474 175
MspA1I CmG'CkG
Cuts at: 0 306 321 413 708 1059 1377
Size: 306 15 92 295 351 318
Fragments arranged by size:
351 318 306 295 92 15
NcoI C'CATG_G
Cuts at: 0 689 1377
Size: 689 688
NspI r_CATG'y
Cuts at: 0 80 680 1183 1377
Size: 80 600 503 194
Fragments arranged by size:
600 503 194 80
PciI A'CATG_T
Cuts at: 0 76 676 1179 1377
Size: 76 600 503 198
Fragments arranged by size:
600 503 198 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 816 1377
Size: 816 561
PstI C_TGCA'G
Cuts at: 0 203 314 1283 1377
Size: 203 111 969 94
Fragments arranged by size:
969 203 111 94
PvuII CAG'CTG
Cuts at: 0 708 1059 1377
Size: 708 351 318
Fragments arranged by size:
708 351 318
SacI G_AGCT'C
Cuts at: 0 857 1346 1377
Size: 857 489 31
Fragments arranged by size:
857 489 31
SapI GCTCTTCn'nnn_
Cuts at: 0 689 1377
Size: 689 688
SfcI C'TryA_G
Cuts at: 0 199 310 582 1279 1377
Size: 199 111 272 697 98
Fragments arranged by size:
697 272 199 111 98
StyI C'CwwG_G
Cuts at: 0 139 216 689 1132 1214 1377
Size: 139 77 473 443 82 163
Fragments arranged by size:
473 443 163 139 82 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 653 1078 1127 1377
Size: 653 425 49 250
Fragments arranged by size:
653 425 250 49
TatI w'GTAC_w
Cuts at: 0 362 1377
Size: 362 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 1377
Size: 757 32 588
Fragments arranged by size:
757 588 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 813 1115 1377
Size: 813 302 262
Fragments arranged by size:
813 302 262
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI AvrII BaeI BamHI
BanII BbvCI BcgI BclI Bme1580I BmgBI BpmI Bpu10I
BsaI BsaAI BsaWI BseRI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BsrGI BstAPI BstYI Bsu36I BtgI
BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoRV FalI
Hin4I HindIII MslI MspA1I NcoI NspI PciI PflMI
PstI PvuII SacI SapI SfcI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BanI BbeI BbsI BciVI BfrBI BglI BglII
BlpI BmrI BmtI BplI BpuEI BsaBI BsaHI BsaXI
BseYI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoO109I EcoRI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI