Restriction map for
pF1KE0951
(Linear) (Six-base) MAPSORT of: KIEE0951.seq Check: 9522 from: 1 to: 1419
KIEE0951.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1342 1419
Size: 1250 92 77
Fragments arranged by size:
1250 92 77
AflII C'TTAA_G
Cuts at: 0 976 1419
Size: 976 443
AflIII A'CryG_T
Cuts at: 0 668 1419
Size: 668 751
AhdI GACnn_n'nnGTC
Cuts at: 0 672 1419
Size: 672 747
ApaI G_GGCC'C
Cuts at: 0 1149 1419
Size: 1149 270
ApoI r'AATT_y
Cuts at: 0 632 758 955 1130 1419
Size: 632 126 197 175 289
Fragments arranged by size:
632 289 197 175 126
AseI AT'TA_AT
Cuts at: 0 838 1419
Size: 838 581
AvaI C'yCGr_G
Cuts at: 0 66 1419
Size: 66 1353
BamHI G'GATC_C
Cuts at: 0 849 1419
Size: 849 570
BanI G'GyrC_C
Cuts at: 0 15 1248 1419
Size: 15 1233 171
Fragments arranged by size:
1233 171 15
BanII G_rGCy'C
Cuts at: 0 1149 1419
Size: 1149 270
BbsI GAAGACnn'nnnn_
Cuts at: 0 601 1330 1419
Size: 601 729 89
Fragments arranged by size:
729 601 89
BciVI GTATCCnnnnn_n'
Cuts at: 0 924 1419
Size: 924 495
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1419
Size: 61 1358
BlpI GC'TnA_GC
Cuts at: 0 124 1175 1419
Size: 124 1051 244
Fragments arranged by size:
1051 244 124
Bme1580I G_kGCm'C
Cuts at: 0 20 1149 1170 1253 1419
Size: 20 1129 21 83 166
Fragments arranged by size:
1129 166 83 21 20
BmtI G_CTAG'C
Cuts at: 0 1067 1419
Size: 1067 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 506 868 1419
Size: 466 40 362 551
Fragments arranged by size:
551 466 362 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 1064 1419
Size: 1064 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 799 1419
Size: 769 30 620
Fragments arranged by size:
769 620 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 1312 1327 1348 1419
Size: 383 929 15 21 71
Fragments arranged by size:
929 383 71 21 15
BseYI C'CCAG_C
Cuts at: 0 153 1419
Size: 153 1266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1235 1419
Size: 1235 184
BsiHKAI G_wGCw'C
Cuts at: 0 590 1419
Size: 590 829
Bsp1286I G_dGCh'C
Cuts at: 0 20 590 1149 1170 1253 1419
Size: 20 570 559 21 83 166
Fragments arranged by size:
570 559 166 83 21 20
BspHI T'CATG_A
Cuts at: 0 590 1419
Size: 590 829
BsrBI CCG'CTC
Cuts at: 0 937 1419
Size: 937 482
BsrGI T'GTAC_A
Cuts at: 0 179 921 1419
Size: 179 742 498
Fragments arranged by size:
742 498 179
BstEII G'GTnAC_C
Cuts at: 0 48 1419
Size: 48 1371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 1260 1419
Size: 825 435 159
Fragments arranged by size:
825 435 159
BstYI r'GATC_y
Cuts at: 0 849 1419
Size: 849 570
Bsu36I CC'TnA_GG
Cuts at: 0 659 1419
Size: 659 760
BtgI C'CryG_G
Cuts at: 0 892 1035 1419
Size: 892 143 384
Fragments arranged by size:
892 384 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1154 1419
Size: 1154 265
BtsI GCAGTG_nn'
Cuts at: 0 1265 1419
Size: 1265 154
EarI CTCTTCn'nnn_
Cuts at: 0 48 1011 1226 1419
Size: 48 963 215 193
Fragments arranged by size:
963 215 193 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 18 1419
Size: 18 1401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 506 868 1250 1342 1419
Size: 466 40 362 382 92 77
Fragments arranged by size:
466 382 362 92 77 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1309 1419
Size: 1309 110
EcoO109I rG'GnC_Cy
Cuts at: 0 200 1145 1236 1302 1419
Size: 200 945 91 66 117
Fragments arranged by size:
945 200 117 91 66
EcoRI G'AATT_C
Cuts at: 0 758 955 1419
Size: 758 197 464
Fragments arranged by size:
758 464 197
FspI TGC'GCA
Cuts at: 0 624 1419
Size: 624 795
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 468 500 658 690 790 822
Size: 130 32 306 32 158 32 100 32
Cuts at: 822 1419
Size: 597
Fragments arranged by size:
597 306 158 130 100 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 973 1419
Size: 973 446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 134 1419
Size: 128 6 1285
Fragments arranged by size:
1285 128 6
MslI CAynn'nnrTG
Cuts at: 0 470 1419
Size: 470 949
MspA1I CmG'CkG
Cuts at: 0 985 1419
Size: 985 434
NcoI C'CATG_G
Cuts at: 0 1035 1419
Size: 1035 384
NheI G'CTAG_C
Cuts at: 0 1063 1419
Size: 1063 356
NspI r_CATG'y
Cuts at: 0 672 1419
Size: 672 747
PasI CC'CwG_GG
Cuts at: 0 613 1419
Size: 613 806
PciI A'CATG_T
Cuts at: 0 668 1419
Size: 668 751
PfoI T'CCnGG_A
Cuts at: 0 86 443 1046 1419
Size: 86 357 603 373
Fragments arranged by size:
603 373 357 86
PpuMI rG'GwC_Cy
Cuts at: 0 200 1419
Size: 200 1219
PsiI TTA'TAA
Cuts at: 0 304 1419
Size: 304 1115
PspOMI G'GGCC_C
Cuts at: 0 1145 1419
Size: 1145 274
PstI C_TGCA'G
Cuts at: 0 426 1419
Size: 426 993
SanDI GG'GwC_CC
Cuts at: 0 200 1419
Size: 200 1219
SapI GCTCTTCn'nnn_
Cuts at: 0 48 1419
Size: 48 1371
SfcI C'TryA_G
Cuts at: 0 332 422 677 1419
Size: 332 90 255 742
Fragments arranged by size:
742 332 255 90
SmaI CCC'GGG
Cuts at: 0 68 1419
Size: 68 1351
SmlI C'TyrA_G
Cuts at: 0 976 1419
Size: 976 443
SspI AAT'ATT
Cuts at: 0 775 1419
Size: 775 644
StyI C'CwwG_G
Cuts at: 0 818 1035 1253 1419
Size: 818 217 218 166
Fragments arranged by size:
818 218 217 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 36 1419
Size: 36 1383
TatI w'GTAC_w
Cuts at: 0 179 921 1419
Size: 179 742 498
Fragments arranged by size:
742 498 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 647 679 1419
Size: 647 32 740
Fragments arranged by size:
740 647 32
Tth111I GACn'n_nGTC
Cuts at: 0 826 1419
Size: 826 593
XmaI C'CCGG_G
Cuts at: 0 66 1419
Size: 66 1353
Enzymes that do cut:
AcuI AflII AflIII AhdI ApaI ApoI AseI AvaI
BamHI BanI BanII BbsI BciVI BglI BlpI Bme1580I
BmtI BpmI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI Hin4I HindIII MmeI MslI MspA1I NcoI
NheI NspI PasI PciI PfoI PpuMI PsiI PspOMI
PstI SanDI SapI SfcI SmaI SmlI SspI StyI
TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglII BmgBI BmrI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoICRI EcoRV FalI FseI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI XbaI XcmI XhoI XmnI ZraI