Restriction map for
pF1KE0954
(Linear) (Six-base) MAPSORT of: KIEE0954.seq Check: 1784 from: 1 to: 1446
KIEE0954.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AclI AA'CG_TT
Cuts at: 0 167 1446
Size: 167 1279
AleI CACnn'nnGTG
Cuts at: 0 1165 1446
Size: 1165 281
AlwNI CAG_nnn'CTG
Cuts at: 0 1040 1446
Size: 1040 406
ApaI G_GGCC'C
Cuts at: 0 191 771 1446
Size: 191 580 675
Fragments arranged by size:
675 580 191
AvaI C'yCGr_G
Cuts at: 0 589 887 1253 1446
Size: 589 298 366 193
Fragments arranged by size:
589 366 298 193
BanI G'GyrC_C
Cuts at: 0 265 667 691 1170 1235 1446
Size: 265 402 24 479 65 211
Fragments arranged by size:
479 402 265 211 65 24
BanII G_rGCy'C
Cuts at: 0 68 191 467 771 1325 1446
Size: 68 123 276 304 554 121
Fragments arranged by size:
554 304 276 123 121 68
BbeI G_GCGC'C
Cuts at: 0 671 1174 1239 1446
Size: 671 503 65 207
Fragments arranged by size:
671 503 207 65
BbvCI CC'TCA_GC
Cuts at: 0 1126 1446
Size: 1126 320
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 1446
Size: 1144 302
BlpI GC'TnA_GC
Cuts at: 0 67 353 604 1446
Size: 67 286 251 842
Fragments arranged by size:
842 286 251 67
Bme1580I G_kGCm'C
Cuts at: 0 191 771 1446
Size: 191 580 675
Fragments arranged by size:
675 580 191
BmgBI CAC'GTC
Cuts at: 0 1062 1446
Size: 1062 384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1221 1446
Size: 1221 225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1446
Size: 499 947
Bpu10I CC'TnA_GC
Cuts at: 0 1126 1446
Size: 1126 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 568 1258 1446
Size: 463 105 690 188
Fragments arranged by size:
690 463 188 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 148 1446
Size: 148 1298
BsaAI yAC'GTr
Cuts at: 0 1167 1446
Size: 1167 279
BsaHI Gr'CG_yC
Cuts at: 0 668 1096 1171 1236 1434 1446
Size: 668 428 75 65 198 12
Fragments arranged by size:
668 428 198 75 65 12
BsaWI w'CCGG_w
Cuts at: 0 818 1446
Size: 818 628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1331 1361 1446
Size: 1331 30 85
Fragments arranged by size:
1331 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1069 1446
Size: 1069 377
BseYI C'CCAG_C
Cuts at: 0 216 922 1303 1309 1446
Size: 216 706 381 6 137
Fragments arranged by size:
706 381 216 137 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1446
Size: 985 461
BsiEI CG_ry'CG
Cuts at: 0 8 1446
Size: 8 1438
BsiHKAI G_wGCw'C
Cuts at: 0 68 467 1446
Size: 68 399 979
Fragments arranged by size:
979 399 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 22 1446
Size: 22 1424
Bsp1286I G_dGCh'C
Cuts at: 0 68 191 467 771 1325 1446
Size: 68 123 276 304 554 121
Fragments arranged by size:
554 304 276 123 121 68
BsrBI CCG'CTC
Cuts at: 0 357 1446
Size: 357 1089
BsrDI GCAATG_nn'
Cuts at: 0 330 1446
Size: 330 1116
BsrFI r'CCGG_y
Cuts at: 0 1098 1446
Size: 1098 348
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 934 1446
Size: 421 513 512
Fragments arranged by size:
513 512 421
Bsu36I CC'TnA_GG
Cuts at: 0 301 1401 1446
Size: 301 1100 45
Fragments arranged by size:
1100 301 45
BtgI C'CryG_G
Cuts at: 0 74 830 1325 1428 1446
Size: 74 756 495 103 18
Fragments arranged by size:
756 495 103 74 18
BtsI GCAGTG_nn'
Cuts at: 0 192 735 859 1446
Size: 192 543 124 587
Fragments arranged by size:
587 543 192 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 1413 1446
Size: 1413 33
EaeI y'GGCC_r
Cuts at: 0 5 732 1446
Size: 5 727 714
Fragments arranged by size:
727 714 5
EagI C'GGCC_G
Cuts at: 0 5 1446
Size: 5 1441
EarI CTCTTCn'nnn_
Cuts at: 0 131 472 1446
Size: 131 341 974
Fragments arranged by size:
974 341 131
EcoICRI GAG'CTC
Cuts at: 0 66 465 1446
Size: 66 399 981
Fragments arranged by size:
981 399 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1446
Size: 499 947
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 1446
Size: 304 1142
EcoO109I rG'GnC_Cy
Cuts at: 0 188 579 767 768 891 1037 1257 1446
Size: 188 391 188 1 123 146 220 189
Fragments arranged by size:
391 220 189 188 188 146 123 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 1218 1446
Size: 402 816 228
Fragments arranged by size:
816 402 228
FspI TGC'GCA
Cuts at: 0 322 727 1446
Size: 322 405 719
Fragments arranged by size:
719 405 322
HaeII r_GCGC'y
Cuts at: 0 671 1174 1239 1446
Size: 671 503 65 207
Fragments arranged by size:
671 503 207 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 1446
Size: 476 32 938
Fragments arranged by size:
938 476 32
KasI G'GCGC_C
Cuts at: 0 667 1170 1235 1446
Size: 667 503 65 211
Fragments arranged by size:
667 503 211 65
MscI TGG'CCA
Cuts at: 0 734 1446
Size: 734 712
MslI CAynn'nnrTG
Cuts at: 0 1165 1446
Size: 1165 281
MspA1I CmG'CkG
Cuts at: 0 76 109 930 1446
Size: 76 33 821 516
Fragments arranged by size:
821 516 76 33
NarI GG'CG_CC
Cuts at: 0 668 1171 1236 1446
Size: 668 503 65 210
Fragments arranged by size:
668 503 210 65
NcoI C'CATG_G
Cuts at: 0 830 1446
Size: 830 616
NspI r_CATG'y
Cuts at: 0 86 786 1446
Size: 86 700 660
Fragments arranged by size:
700 660 86
PasI CC'CwG_GG
Cuts at: 0 543 561 946 1446
Size: 543 18 385 500
Fragments arranged by size:
543 500 385 18
PfoI T'CCnGG_A
Cuts at: 0 497 1181 1446
Size: 497 684 265
Fragments arranged by size:
684 497 265
PpuMI rG'GwC_Cy
Cuts at: 0 579 891 1257 1446
Size: 579 312 366 189
Fragments arranged by size:
579 366 312 189
PshAI GACnn'nnGTC
Cuts at: 0 83 1446
Size: 83 1363
PspOMI G'GGCC_C
Cuts at: 0 187 767 1446
Size: 187 580 679
Fragments arranged by size:
679 580 187
PstI C_TGCA'G
Cuts at: 0 1049 1446
Size: 1049 397
PvuII CAG'CTG
Cuts at: 0 109 1446
Size: 109 1337
SacI G_AGCT'C
Cuts at: 0 68 467 1446
Size: 68 399 979
Fragments arranged by size:
979 399 68
SacII CC_GC'GG
Cuts at: 0 77 1446
Size: 77 1369
SanDI GG'GwC_CC
Cuts at: 0 1257 1446
Size: 1257 189
SapI GCTCTTCn'nnn_
Cuts at: 0 472 1446
Size: 472 974
SfcI C'TryA_G
Cuts at: 0 1045 1446
Size: 1045 401
SfoI GGC'GCC
Cuts at: 0 669 1172 1237 1446
Size: 669 503 65 209
Fragments arranged by size:
669 503 209 65
SgrAI Cr'CCGG_yG
Cuts at: 0 1098 1446
Size: 1098 348
SmaI CCC'GGG
Cuts at: 0 889 1255 1446
Size: 889 366 191
Fragments arranged by size:
889 366 191
SmlI C'TyrA_G
Cuts at: 0 442 583 1273 1446
Size: 442 141 690 173
Fragments arranged by size:
690 442 173 141
StuI AGG'CCT
Cuts at: 0 1147 1446
Size: 1147 299
StyI C'CwwG_G
Cuts at: 0 329 830 1446
Size: 329 501 616
Fragments arranged by size:
616 501 329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 1245 1446
Size: 646 599 201
Fragments arranged by size:
646 599 201
TatI w'GTAC_w
Cuts at: 0 701 1446
Size: 701 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 924 956 1446
Size: 924 32 490
Fragments arranged by size:
924 490 32
Tth111I GACn'n_nGTC
Cuts at: 0 895 1446
Size: 895 551
XbaI T'CTAG_A
Cuts at: 0 366 1446
Size: 366 1080
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 1446
Size: 702 744
XmaI C'CCGG_G
Cuts at: 0 887 1253 1446
Size: 887 366 193
Fragments arranged by size:
887 366 193
Enzymes that do cut:
AclI AleI AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI
BstXI Bsu36I BtgI BtsI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PasI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SanDI SapI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BsaBI BsiWI BsmI BspEI
BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EciI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI XhoI XmnI ZraI