Restriction map for
pF1KE0960
(Linear) (Six-base) MAPSORT of: KIEE0960.seq Check: 8918 from: 1 to: 1497
KIEE0960.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 977 1497
Size: 977 520
Acc65I G'GTAC_C
Cuts at: 0 17 1497
Size: 17 1480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1405 1497
Size: 595 810 92
Fragments arranged by size:
810 595 92
AflIII A'CryG_T
Cuts at: 0 713 1497
Size: 713 784
AleI CACnn'nnGTG
Cuts at: 0 964 1497
Size: 964 533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 1327 1497
Size: 982 345 170
Fragments arranged by size:
982 345 170
ApoI r'AATT_y
Cuts at: 0 897 1497
Size: 897 600
AvaI C'yCGr_G
Cuts at: 0 563 1497
Size: 563 934
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 702 735 1497
Size: 702 33 762
Fragments arranged by size:
762 702 33
BanI G'GyrC_C
Cuts at: 0 17 1497
Size: 17 1480
BanII G_rGCy'C
Cuts at: 0 564 914 1497
Size: 564 350 583
Fragments arranged by size:
583 564 350
BbvCI CC'TCA_GC
Cuts at: 0 440 1497
Size: 440 1057
BlpI GC'TnA_GC
Cuts at: 0 1394 1451 1497
Size: 1394 57 46
Fragments arranged by size:
1394 57 46
BmgBI CAC'GTC
Cuts at: 0 462 1497
Size: 462 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1461 1497
Size: 1461 36
Bpu10I CC'TnA_GC
Cuts at: 0 440 742 1497
Size: 440 302 755
Fragments arranged by size:
755 440 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 856 1081 1497
Size: 640 216 225 416
Fragments arranged by size:
640 416 225 216
BsaHI Gr'CG_yC
Cuts at: 0 132 1497
Size: 132 1365
BsaWI w'CCGG_w
Cuts at: 0 119 194 1497
Size: 119 75 1303
Fragments arranged by size:
1303 119 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 39 69 1497
Size: 39 30 1428
Fragments arranged by size:
1428 39 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 378 1497
Size: 15 363 1119
Fragments arranged by size:
1119 363 15
BseYI C'CCAG_C
Cuts at: 0 1279 1497
Size: 1279 218
BsiEI CG_ry'CG
Cuts at: 0 195 525 1497
Size: 195 330 972
Fragments arranged by size:
972 330 195
BsiHKAI G_wGCw'C
Cuts at: 0 914 961 1010 1047 1497
Size: 914 47 49 37 450
Fragments arranged by size:
914 450 49 47 37
BsmI GAATG_Cn'
Cuts at: 0 288 1497
Size: 288 1209
Bsp1286I G_dGCh'C
Cuts at: 0 564 914 961 1010 1047 1497
Size: 564 350 47 49 37 450
Fragments arranged by size:
564 450 350 49 47 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 785 1497
Size: 785 712
BsrBI CCG'CTC
Cuts at: 0 389 1497
Size: 389 1108
BsrFI r'CCGG_y
Cuts at: 0 209 1497
Size: 209 1288
BsrGI T'GTAC_A
Cuts at: 0 531 1497
Size: 531 966
BstYI r'GATC_y
Cuts at: 0 1184 1497
Size: 1184 313
Bsu36I CC'TnA_GG
Cuts at: 0 504 1497
Size: 504 993
BtgI C'CryG_G
Cuts at: 0 705 1497
Size: 705 792
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 532 1497
Size: 532 965
BtsI GCAGTG_nn'
Cuts at: 0 954 993 1497
Size: 954 39 504
Fragments arranged by size:
954 504 39
DraIII CAC_nnn'GTG
Cuts at: 0 798 1497
Size: 798 699
DrdI GACnn_nn'nnGTC
Cuts at: 0 92 1497
Size: 92 1405
EaeI y'GGCC_r
Cuts at: 0 522 708 1497
Size: 522 186 789
Fragments arranged by size:
789 522 186
EagI C'GGCC_G
Cuts at: 0 522 1497
Size: 522 975
EarI CTCTTCn'nnn_
Cuts at: 0 949 1144 1360 1497
Size: 949 195 216 137
Fragments arranged by size:
949 216 195 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 375 1497
Size: 375 1122
EcoICRI GAG'CTC
Cuts at: 0 912 1497
Size: 912 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1405 1461 1497
Size: 595 810 56 36
Fragments arranged by size:
810 595 56 36
EcoO109I rG'GnC_Cy
Cuts at: 0 515 1192 1497
Size: 515 677 305
Fragments arranged by size:
677 515 305
EcoRI G'AATT_C
Cuts at: 0 897 1497
Size: 897 600
EcoRV GAT'ATC
Cuts at: 0 1488 1497
Size: 1488 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1075 1497
Size: 1075 422
HaeII r_GCGC'y
Cuts at: 0 1287 1497
Size: 1287 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 476 508 608 640 1121 1153
Size: 172 32 272 32 100 32 481 32
Cuts at: 1153 1176 1208 1258 1290 1497
Size: 23 32 50 32 207
Fragments arranged by size:
481 272 207 172 100 50 32 32
32 32 32 32 23
HincII GTy'rAC
Cuts at: 0 543 1497
Size: 543 954
KpnI G_GTAC'C
Cuts at: 0 21 1497
Size: 21 1476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1497
Size: 215 1282
MscI TGG'CCA
Cuts at: 0 710 1497
Size: 710 787
MslI CAynn'nnrTG
Cuts at: 0 293 964 1497
Size: 293 671 533
Fragments arranged by size:
671 533 293
MspA1I CmG'CkG
Cuts at: 0 306 819 964 1279 1311 1497
Size: 306 513 145 315 32 186
Fragments arranged by size:
513 315 306 186 145 32
NcoI C'CATG_G
Cuts at: 0 705 1497
Size: 705 792
NspI r_CATG'y
Cuts at: 0 717 1377 1497
Size: 717 660 120
Fragments arranged by size:
717 660 120
PciI A'CATG_T
Cuts at: 0 713 1497
Size: 713 784
PfoI T'CCnGG_A
Cuts at: 0 1204 1497
Size: 1204 293
PpuMI rG'GwC_Cy
Cuts at: 0 515 1192 1497
Size: 515 677 305
Fragments arranged by size:
677 515 305
PshAI GACnn'nnGTC
Cuts at: 0 1478 1497
Size: 1478 19
PspXI vC'TCGA_Gb
Cuts at: 0 563 1497
Size: 563 934
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 304 336 1497
Size: 304 32 1161
Fragments arranged by size:
1161 304 32
PvuII CAG'CTG
Cuts at: 0 819 964 1279 1497
Size: 819 145 315 218
Fragments arranged by size:
819 315 218 145
SacI G_AGCT'C
Cuts at: 0 914 1497
Size: 914 583
SapI GCTCTTCn'nnn_
Cuts at: 0 949 1360 1497
Size: 949 411 137
Fragments arranged by size:
949 411 137
SfcI C'TryA_G
Cuts at: 0 228 1497
Size: 228 1269
SmlI C'TyrA_G
Cuts at: 0 563 619 871 1060 1497
Size: 563 56 252 189 437
Fragments arranged by size:
563 437 252 189 56
SphI G_CATG'C
Cuts at: 0 1377 1497
Size: 1377 120
SspI AAT'ATT
Cuts at: 0 885 1237 1497
Size: 885 352 260
Fragments arranged by size:
885 352 260
StyI C'CwwG_G
Cuts at: 0 705 1406 1497
Size: 705 701 91
Fragments arranged by size:
705 701 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1399 1497
Size: 1399 98
TatI w'GTAC_w
Cuts at: 0 531 599 1497
Size: 531 68 898
Fragments arranged by size:
898 531 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 1497
Size: 39 32 1426
Fragments arranged by size:
1426 39 32
Tth111I GACn'n_nGTC
Cuts at: 0 131 1497
Size: 131 1366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 553 1497
Size: 553 944
XhoI C'TCGA_G
Cuts at: 0 563 1497
Size: 563 934
XmnI GAAnn'nnTTC
Cuts at: 0 884 1418 1497
Size: 884 534 79
Fragments arranged by size:
884 534 79
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI ApoI AvaI
BaeI BanI BanII BbvCI BlpI BmgBI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
NspI PciI PfoI PpuMI PshAI PspXI PsrI PvuII
SacI SapI SfcI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
Bme1580I BmrI BmtI BplI BsaI BsaAI BsaBI BsgI
BsiWI BsmBI BspEI BspHI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EcoNI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PsiI PspOMI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI XbaI XmaI ZraI