Restriction map for
pF1KE0965
(Linear) (Six-base) MAPSORT of: KIEE0965.seq Check: 5607 from: 1 to: 1527
KIEE0965.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 0 194 1527
Size: 194 1333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 868 1527
Size: 785 83 659
Fragments arranged by size:
785 659 83
AfeI AGC'GCT
Cuts at: 0 1138 1527
Size: 1138 389
AflIII A'CryG_T
Cuts at: 0 98 1527
Size: 98 1429
ApoI r'AATT_y
Cuts at: 0 354 442 1527
Size: 354 88 1085
Fragments arranged by size:
1085 354 88
AvaI C'yCGr_G
Cuts at: 0 476 1527
Size: 476 1051
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 810 843 1527
Size: 810 33 684
Fragments arranged by size:
810 684 33
BanI G'GyrC_C
Cuts at: 0 1129 1527
Size: 1129 398
BanII G_rGCy'C
Cuts at: 0 509 1527
Size: 509 1018
BbsI GAAGACnn'nnnn_
Cuts at: 0 577 1527
Size: 577 950
BbvCI CC'TCA_GC
Cuts at: 0 769 1527
Size: 769 758
BciVI GTATCCnnnnn_n'
Cuts at: 0 894 1527
Size: 894 633
BfrBI ATG'CAT
Cuts at: 0 1080 1527
Size: 1080 447
BglII A'GATC_T
Cuts at: 0 704 1527
Size: 704 823
Bme1580I G_kGCm'C
Cuts at: 0 1132 1527
Size: 1132 395
BmgBI CAC'GTC
Cuts at: 0 192 483 1527
Size: 192 291 1044
Fragments arranged by size:
1044 291 192
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 451 1527
Size: 451 1076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 1527
Size: 807 720
Bpu10I CC'TnA_GC
Cuts at: 0 769 1527
Size: 769 758
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 1527
Size: 121 1406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 702 1527
Size: 702 825
BseYI C'CCAG_C
Cuts at: 0 314 423 830 1527
Size: 314 109 407 697
Fragments arranged by size:
697 407 314 109
BsiHKAI G_wGCw'C
Cuts at: 0 1093 1527
Size: 1093 434
BsmI GAATG_Cn'
Cuts at: 0 1080 1432 1527
Size: 1080 352 95
Fragments arranged by size:
1080 352 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 473 1527
Size: 473 1054
Bsp1286I G_dGCh'C
Cuts at: 0 509 1093 1132 1527
Size: 509 584 39 395
Fragments arranged by size:
584 509 395 39
BspHI T'CATG_A
Cuts at: 0 35 855 1527
Size: 35 820 672
Fragments arranged by size:
820 672 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 641 1527
Size: 641 886
BsrDI GCAATG_nn'
Cuts at: 0 794 992 1266 1527
Size: 794 198 274 261
Fragments arranged by size:
794 274 261 198
BsrGI T'GTAC_A
Cuts at: 0 562 752 1527
Size: 562 190 775
Fragments arranged by size:
775 562 190
BstBI TT'CG_AA
Cuts at: 0 918 1527
Size: 918 609
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1176 1251 1527
Size: 1176 75 276
Fragments arranged by size:
1176 276 75
BstYI r'GATC_y
Cuts at: 0 704 1527
Size: 704 823
BtgI C'CryG_G
Cuts at: 0 269 1527
Size: 269 1258
BtsI GCAGTG_nn'
Cuts at: 0 160 1462 1527
Size: 160 1302 65
Fragments arranged by size:
1302 160 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 504 539 1527
Size: 504 35 988
Fragments arranged by size:
988 504 35
DraI TTT'AAA
Cuts at: 0 447 1010 1527
Size: 447 563 517
Fragments arranged by size:
563 517 447
DrdI GACnn_nn'nnGTC
Cuts at: 0 252 1527
Size: 252 1275
EaeI y'GGCC_r
Cuts at: 0 261 1241 1409 1527
Size: 261 980 168 118
Fragments arranged by size:
980 261 168 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1263 1527
Size: 1263 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 807 868 1527
Size: 785 22 61 659
Fragments arranged by size:
785 659 61 22
EcoO109I rG'GnC_Cy
Cuts at: 0 974 1527
Size: 974 553
HaeII r_GCGC'y
Cuts at: 0 1140 1527
Size: 1140 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 796 828 1080 1112 1527
Size: 712 32 52 32 252 32 415
Fragments arranged by size:
712 415 252 52 32 32 32
HincII GTy'rAC
Cuts at: 0 84 1527
Size: 84 1443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 611 1527
Size: 504 107 916
Fragments arranged by size:
916 504 107
MscI TGG'CCA
Cuts at: 0 263 1243 1411 1527
Size: 263 980 168 116
Fragments arranged by size:
980 263 168 116
MslI CAynn'nnrTG
Cuts at: 0 103 763 854 1174 1527
Size: 103 660 91 320 353
Fragments arranged by size:
660 353 320 103 91
MspA1I CmG'CkG
Cuts at: 0 318 677 834 1527
Size: 318 359 157 693
Fragments arranged by size:
693 359 318 157
NsiI A_TGCA'T
Cuts at: 0 1082 1527
Size: 1082 445
NspI r_CATG'y
Cuts at: 0 102 1527
Size: 102 1425
PciI A'CATG_T
Cuts at: 0 98 1527
Size: 98 1429
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 1379 1527
Size: 270 1109 148
Fragments arranged by size:
1109 270 148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 629 1527
Size: 597 32 898
Fragments arranged by size:
898 597 32
PpuMI rG'GwC_Cy
Cuts at: 0 974 1527
Size: 974 553
PsiI TTA'TAA
Cuts at: 0 1196 1527
Size: 1196 331
PvuII CAG'CTG
Cuts at: 0 318 677 834 1527
Size: 318 359 157 693
Fragments arranged by size:
693 359 318 157
SanDI GG'GwC_CC
Cuts at: 0 974 1527
Size: 974 553
SfcI C'TryA_G
Cuts at: 0 389 1527
Size: 389 1138
SspI AAT'ATT
Cuts at: 0 1023 1527
Size: 1023 504
StyI C'CwwG_G
Cuts at: 0 77 282 978 1527
Size: 77 205 696 549
Fragments arranged by size:
696 549 205 77
TatI w'GTAC_w
Cuts at: 0 562 752 1527
Size: 562 190 775
Fragments arranged by size:
775 562 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 341 373 544 576 1527
Size: 341 32 171 32 951
Fragments arranged by size:
951 341 171 32 32
XbaI T'CTAG_A
Cuts at: 0 1158 1527
Size: 1158 369
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 1140 1527
Size: 367 773 387
Fragments arranged by size:
773 387 367
XmnI GAAnn'nnTTC
Cuts at: 0 706 1527
Size: 706 821
Enzymes that do cut:
AccI AcuI AfeI AflIII ApoI AvaI BaeI BanI
BanII BbsI BbvCI BciVI BfrBI BglII Bme1580I BmgBI
BplI BpmI Bpu10I BsaI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstBI BstXI
BstYI BtgI BtsI CspCI DraI DrdI EaeI EciI
Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI MslI
MspA1I NsiI NspI PciI PflMI PpiI PpuMI PsiI
PvuII SanDI SfcI SspI StyI TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BcgI BclI BglI BlpI BmrI
BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgZI ClaI DraIII EagI EarI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII Tth111I XhoI
XmaI ZraI