Restriction map for
pF1KE0968
(Linear) (Six-base) MAPSORT of: KIEE0968.seq Check: 9547 from: 1 to: 1560
KIEE0968.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 782 1448 1560
Size: 782 666 112
Fragments arranged by size:
782 666 112
AclI AA'CG_TT
Cuts at: 0 1541 1560
Size: 1541 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 298 466 588 1560
Size: 8 290 168 122 972
Fragments arranged by size:
972 290 168 122 8
AflIII A'CryG_T
Cuts at: 0 785 857 1560
Size: 785 72 703
Fragments arranged by size:
785 703 72
AleI CACnn'nnGTG
Cuts at: 0 1090 1560
Size: 1090 470
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 1560
Size: 175 32 1353
Fragments arranged by size:
1353 175 32
AlwNI CAG_nnn'CTG
Cuts at: 0 435 1242 1560
Size: 435 807 318
Fragments arranged by size:
807 435 318
ApoI r'AATT_y
Cuts at: 0 235 1560
Size: 235 1325
AvaI C'yCGr_G
Cuts at: 0 913 1308 1560
Size: 913 395 252
Fragments arranged by size:
913 395 252
BamHI G'GATC_C
Cuts at: 0 60 1560
Size: 60 1500
BanII G_rGCy'C
Cuts at: 0 17 554 1560
Size: 17 537 1006
Fragments arranged by size:
1006 537 17
BclI T'GATC_A
Cuts at: 0 291 1392 1560
Size: 291 1101 168
Fragments arranged by size:
1101 291 168
BmgBI CAC'GTC
Cuts at: 0 846 1560
Size: 846 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 978 1393 1560
Size: 340 638 415 167
Fragments arranged by size:
638 415 340 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 676 1168 1201 1513 1560
Size: 1 675 492 33 312 47
Fragments arranged by size:
675 492 312 47 33 1
BsaAI yAC'GTr
Cuts at: 0 788 1560
Size: 788 772
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1560
Size: 175 30 1355
Fragments arranged by size:
1355 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 1560
Size: 88 1472
BseYI C'CCAG_C
Cuts at: 0 957 1560
Size: 957 603
BsiHKAI G_wGCw'C
Cuts at: 0 17 554 1560
Size: 17 537 1006
Fragments arranged by size:
1006 537 17
BsmI GAATG_Cn'
Cuts at: 0 1383 1560
Size: 1383 177
Bsp1286I G_dGCh'C
Cuts at: 0 17 554 1560
Size: 17 537 1006
Fragments arranged by size:
1006 537 17
BsrDI GCAATG_nn'
Cuts at: 0 296 1560
Size: 296 1264
BsrFI r'CCGG_y
Cuts at: 0 3 800 973 1560
Size: 3 797 173 587
Fragments arranged by size:
797 587 173 3
BsrGI T'GTAC_A
Cuts at: 0 890 1560
Size: 890 670
BstXI CCAn_nnnn'nTGG
Cuts at: 0 363 1119 1560
Size: 363 756 441
Fragments arranged by size:
756 441 363
BstYI r'GATC_y
Cuts at: 0 60 449 1560
Size: 60 389 1111
Fragments arranged by size:
1111 389 60
BstZ17I GTA'TAC
Cuts at: 0 783 1560
Size: 783 777
Bsu36I CC'TnA_GG
Cuts at: 0 1243 1560
Size: 1243 317
EaeI y'GGCC_r
Cuts at: 0 353 848 1560
Size: 353 495 712
Fragments arranged by size:
712 495 353
EarI CTCTTCn'nnn_
Cuts at: 0 409 1163 1560
Size: 409 754 397
Fragments arranged by size:
754 409 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 898 1498 1560
Size: 898 600 62
Fragments arranged by size:
898 600 62
EcoICRI GAG'CTC
Cuts at: 0 15 552 1560
Size: 15 537 1008
Fragments arranged by size:
1008 537 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 298 340 466 588 978 1393 1560
Size: 8 290 42 126 122 390 415 167
Fragments arranged by size:
415 390 290 167 126 122 42 8
EcoO109I rG'GnC_Cy
Cuts at: 0 120 282 338 432 1080 1179 1232 1560
Size: 120 162 56 94 648 99 53 328
Fragments arranged by size:
648 328 162 120 99 94 56 53
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 1560
Size: 357 1203
HaeII r_GCGC'y
Cuts at: 0 627 1560
Size: 627 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 739 771 1560
Size: 64 32 643 32 789
Fragments arranged by size:
789 643 64 32 32
HindIII A'AGCT_T
Cuts at: 0 127 1560
Size: 127 1433
MscI TGG'CCA
Cuts at: 0 355 850 1560
Size: 355 495 710
Fragments arranged by size:
710 495 355
MslI CAynn'nnrTG
Cuts at: 0 856 952 1090 1388 1560
Size: 856 96 138 298 172
Fragments arranged by size:
856 298 172 138 96
MspA1I CmG'CkG
Cuts at: 0 112 144 936 1434 1474 1560
Size: 112 32 792 498 40 86
Fragments arranged by size:
792 498 112 86 40 32
NaeI GCC'GGC
Cuts at: 0 5 975 1560
Size: 5 970 585
Fragments arranged by size:
970 585 5
NdeI CA'TA_TG
Cuts at: 0 153 503 1560
Size: 153 350 1057
Fragments arranged by size:
1057 350 153
NgoMIV G'CCGG_C
Cuts at: 0 3 973 1560
Size: 3 970 587
Fragments arranged by size:
970 587 3
NspI r_CATG'y
Cuts at: 0 861 1192 1378 1560
Size: 861 331 186 182
Fragments arranged by size:
861 331 186 182
PciI A'CATG_T
Cuts at: 0 857 1560
Size: 857 703
PfoI T'CCnGG_A
Cuts at: 0 452 994 1118 1560
Size: 452 542 124 442
Fragments arranged by size:
542 452 442 124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1560
Size: 175 32 1353
Fragments arranged by size:
1353 175 32
PpuMI rG'GwC_Cy
Cuts at: 0 282 338 1080 1560
Size: 282 56 742 480
Fragments arranged by size:
742 480 282 56
PspXI vC'TCGA_Gb
Cuts at: 0 1308 1560
Size: 1308 252
PvuII CAG'CTG
Cuts at: 0 144 936 1434 1474 1560
Size: 144 792 498 40 86
Fragments arranged by size:
792 498 144 86 40
SacI G_AGCT'C
Cuts at: 0 17 554 1560
Size: 17 537 1006
Fragments arranged by size:
1006 537 17
SfcI C'TryA_G
Cuts at: 0 1343 1560
Size: 1343 217
SmaI CCC'GGG
Cuts at: 0 915 1560
Size: 915 645
SmlI C'TyrA_G
Cuts at: 0 16 691 1183 1216 1308 1528 1560
Size: 16 675 492 33 92 220 32
Fragments arranged by size:
675 492 220 92 33 32 16
SphI G_CATG'C
Cuts at: 0 1192 1560
Size: 1192 368
StyI C'CwwG_G
Cuts at: 0 65 1076 1560
Size: 65 1011 484
Fragments arranged by size:
1011 484 65
TatI w'GTAC_w
Cuts at: 0 890 1560
Size: 890 670
XhoI C'TCGA_G
Cuts at: 0 1308 1560
Size: 1308 252
XmaI C'CCGG_G
Cuts at: 0 913 1560
Size: 913 647
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AloI AlwNI ApoI
AvaI BamHI BanII BclI BmgBI BpmI BpuEI BsaAI
BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BsrGI BstXI BstYI BstZ17I Bsu36I EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII MscI
MslI MspA1I NaeI NdeI NgoMIV NspI PciI PfoI
PpiI PpuMI PspXI PvuII SacI SfcI SmaI SmlI
SphI StyI TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XmnI ZraI