Restriction map for
pF1KE0971
(Linear) (Six-base) MAPSORT of: KIEE0971.seq Check: 9181 from: 1 to: 1620
KIEE0971.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 152 1620
Size: 152 1468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1534 1620
Size: 1229 305 86
Fragments arranged by size:
1229 305 86
AflII C'TTAA_G
Cuts at: 0 189 1620
Size: 189 1431
AleI CACnn'nnGTG
Cuts at: 0 968 1620
Size: 968 652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 43 398 1620
Size: 43 355 1222
Fragments arranged by size:
1222 355 43
ApoI r'AATT_y
Cuts at: 0 203 248 265 469 1498 1596 1620
Size: 203 45 17 204 1029 98 24
Fragments arranged by size:
1029 204 203 98 45 24 17
AseI AT'TA_AT
Cuts at: 0 749 1620
Size: 749 871
BamHI G'GATC_C
Cuts at: 0 1039 1316 1620
Size: 1039 277 304
Fragments arranged by size:
1039 304 277
BanI G'GyrC_C
Cuts at: 0 79 1620
Size: 79 1541
BbeI G_GCGC'C
Cuts at: 0 83 1620
Size: 83 1537
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 1340 1540 1620
Size: 448 892 200 80
Fragments arranged by size:
892 448 200 80
BclI T'GATC_A
Cuts at: 0 1221 1403 1620
Size: 1221 182 217
Fragments arranged by size:
1221 217 182
Bme1580I G_kGCm'C
Cuts at: 0 822 1620
Size: 822 798
Bpu10I CC'TnA_GC
Cuts at: 0 70 1620
Size: 70 1550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 1115 1620
Size: 1085 30 505
Fragments arranged by size:
1085 505 30
BsaAI yAC'GTr
Cuts at: 0 790 1620
Size: 790 830
BsaBI GATnn'nnATC
Cuts at: 0 1587 1620
Size: 1587 33
BsaHI Gr'CG_yC
Cuts at: 0 80 1620
Size: 80 1540
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 407 1088 1620
Size: 407 681 532
Fragments arranged by size:
681 532 407
BseYI C'CCAG_C
Cuts at: 0 1307 1620
Size: 1307 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 388 533 1620
Size: 130 258 145 1087
Fragments arranged by size:
1087 258 145 130
BsiHKAI G_wGCw'C
Cuts at: 0 789 1620
Size: 789 831
BsmI GAATG_Cn'
Cuts at: 0 1213 1620
Size: 1213 407
Bsp1286I G_dGCh'C
Cuts at: 0 789 822 1620
Size: 789 33 798
Fragments arranged by size:
798 789 33
BspHI T'CATG_A
Cuts at: 0 432 1406 1620
Size: 432 974 214
Fragments arranged by size:
974 432 214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 152 410 1620
Size: 68 84 258 1210
Fragments arranged by size:
1210 258 84 68
BsrDI GCAATG_nn'
Cuts at: 0 261 1620
Size: 261 1359
BstYI r'GATC_y
Cuts at: 0 1039 1198 1316 1620
Size: 1039 159 118 304
Fragments arranged by size:
1039 304 159 118
BtgI C'CryG_G
Cuts at: 0 75 1620
Size: 75 1545
BtsI GCAGTG_nn'
Cuts at: 0 1318 1620
Size: 1318 302
DraI TTT'AAA
Cuts at: 0 1595 1620
Size: 1595 25
DrdI GACnn_nn'nnGTC
Cuts at: 0 121 1620
Size: 121 1499
EaeI y'GGCC_r
Cuts at: 0 131 352 1620
Size: 131 221 1268
Fragments arranged by size:
1268 221 131
EarI CTCTTCn'nnn_
Cuts at: 0 887 1248 1620
Size: 887 361 372
Fragments arranged by size:
887 372 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 1620
Size: 90 1530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1534 1620
Size: 1229 305 86
Fragments arranged by size:
1229 305 86
EcoO109I rG'GnC_Cy
Cuts at: 0 1085 1448 1620
Size: 1085 363 172
Fragments arranged by size:
1085 363 172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 860 1381 1620
Size: 204 656 521 239
Fragments arranged by size:
656 521 239 204
HaeII r_GCGC'y
Cuts at: 0 26 83 1620
Size: 26 57 1537
Fragments arranged by size:
1537 57 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 1102 1134 1620
Size: 353 32 717 32 486
Fragments arranged by size:
717 486 353 32 32
KasI G'GCGC_C
Cuts at: 0 79 1620
Size: 79 1541
MscI TGG'CCA
Cuts at: 0 354 1620
Size: 354 1266
MslI CAynn'nnrTG
Cuts at: 0 968 1620
Size: 968 652
MspA1I CmG'CkG
Cuts at: 0 77 162 1620
Size: 77 85 1458
Fragments arranged by size:
1458 85 77
NarI GG'CG_CC
Cuts at: 0 80 1620
Size: 80 1540
NdeI CA'TA_TG
Cuts at: 0 768 1620
Size: 768 852
NspI r_CATG'y
Cuts at: 0 294 1620
Size: 294 1326
PfoI T'CCnGG_A
Cuts at: 0 1164 1620
Size: 1164 456
PpuMI rG'GwC_Cy
Cuts at: 0 1085 1448 1620
Size: 1085 363 172
Fragments arranged by size:
1085 363 172
PstI C_TGCA'G
Cuts at: 0 22 1275 1295 1620
Size: 22 1253 20 325
Fragments arranged by size:
1253 325 22 20
SacII CC_GC'GG
Cuts at: 0 78 1620
Size: 78 1542
SapI GCTCTTCn'nnn_
Cuts at: 0 1248 1620
Size: 1248 372
SfcI C'TryA_G
Cuts at: 0 18 1271 1291 1620
Size: 18 1253 20 329
Fragments arranged by size:
1253 329 20 18
SfoI GGC'GCC
Cuts at: 0 81 1620
Size: 81 1539
SmlI C'TyrA_G
Cuts at: 0 189 1100 1130 1620
Size: 189 911 30 490
Fragments arranged by size:
911 490 189 30
StyI C'CwwG_G
Cuts at: 0 387 1488 1620
Size: 387 1101 132
Fragments arranged by size:
1101 387 132
TatI w'GTAC_w
Cuts at: 0 1515 1620
Size: 1515 105
XbaI T'CTAG_A
Cuts at: 0 1120 1579 1620
Size: 1120 459 41
Fragments arranged by size:
1120 459 41
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1060 1620
Size: 1060 560
Enzymes that do cut:
AarI AcuI AflII AleI AlfI ApoI AseI BamHI
BanI BbeI BbsI BclI Bme1580I Bpu10I BpuEI BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BstYI BtgI BtsI DraI DrdI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MscI MslI MspA1I NarI NdeI NspI PfoI
PpuMI PstI SacII SapI SfcI SfoI SmlI StyI
TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanII BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BsaI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XhoI XmaI XmnI
ZraI