Restriction map for
pF1KE0975
(Linear) (Six-base) MAPSORT of: KIEE0975.seq Check: 3306 from: 1 to: 1632
KIEE0975.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1109 1613 1632
Size: 1109 504 19
Fragments arranged by size:
1109 504 19
AatII G_ACGT'C
Cuts at: 0 335 1632
Size: 335 1297
Acc65I G'GTAC_C
Cuts at: 0 545 1632
Size: 545 1087
AclI AA'CG_TT
Cuts at: 0 1226 1632
Size: 1226 406
AflIII A'CryG_T
Cuts at: 0 599 1632
Size: 599 1033
AleI CACnn'nnGTG
Cuts at: 0 10 1632
Size: 10 1622
AlwNI CAG_nnn'CTG
Cuts at: 0 363 792 894 1401 1632
Size: 363 429 102 507 231
Fragments arranged by size:
507 429 363 231 102
ApaI G_GGCC'C
Cuts at: 0 1072 1632
Size: 1072 560
ApaLI G'TGCA_C
Cuts at: 0 3 400 1632
Size: 3 397 1232
Fragments arranged by size:
1232 397 3
AvaI C'yCGr_G
Cuts at: 0 55 387 965 1056 1632
Size: 55 332 578 91 576
Fragments arranged by size:
578 576 332 91 55
AvrII C'CTAG_G
Cuts at: 0 1269 1632
Size: 1269 363
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 529 562 1632
Size: 529 33 1070
Fragments arranged by size:
1070 529 33
BanI G'GyrC_C
Cuts at: 0 545 1051 1525 1537 1622 1632
Size: 545 506 474 12 85 10
Fragments arranged by size:
545 506 474 85 12 10
BanII G_rGCy'C
Cuts at: 0 1072 1632
Size: 1072 560
BbvCI CC'TCA_GC
Cuts at: 0 1124 1632
Size: 1124 508
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 1632
Size: 628 1004
BglI GCCn_nnn'nGGC
Cuts at: 0 1396 1632
Size: 1396 236
Bme1580I G_kGCm'C
Cuts at: 0 7 395 404 945 972 1072 1530 1625
Size: 7 388 9 541 27 100 458 95
Cuts at: 1625 1632
Size: 7
Fragments arranged by size:
541 458 388 100 95 27 9 7
7
BmgBI CAC'GTC
Cuts at: 0 234 662 1632
Size: 234 428 970
Fragments arranged by size:
970 428 234
BmrI ACTGGGnnnn_n'
Cuts at: 0 1606 1632
Size: 1606 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1098 1632
Size: 1098 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 700 712 1051 1632
Size: 175 525 12 339 581
Fragments arranged by size:
581 525 339 175 12
Bpu10I CC'TnA_GC
Cuts at: 0 738 1124 1382 1632
Size: 738 386 258 250
Fragments arranged by size:
738 386 258 250
BsaI GGTCTCn'nnnn_
Cuts at: 0 636 688 1632
Size: 636 52 944
Fragments arranged by size:
944 636 52
BsaAI yAC'GTr
Cuts at: 0 339 531 909 1632
Size: 339 192 378 723
Fragments arranged by size:
723 378 339 192
BsaHI Gr'CG_yC
Cuts at: 0 332 1632
Size: 332 1300
BsaWI w'CCGG_w
Cuts at: 0 29 95 308 555 1476 1632
Size: 29 66 213 247 921 156
Fragments arranged by size:
921 247 213 156 66 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 328 358 1632
Size: 90 30 208 30 1274
Fragments arranged by size:
1274 208 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 730 1632
Size: 178 552 902
Fragments arranged by size:
902 552 178
BseYI C'CCAG_C
Cuts at: 0 768 928 1632
Size: 768 160 704
Fragments arranged by size:
768 704 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 745 931 1087 1168 1235 1632
Size: 429 316 186 156 81 67 397
Fragments arranged by size:
429 397 316 186 156 81 67
BsiEI CG_ry'CG
Cuts at: 0 1494 1632
Size: 1494 138
BsiHKAI G_wGCw'C
Cuts at: 0 7 404 431 555 1202 1632
Size: 7 397 27 124 647 430
Fragments arranged by size:
647 430 397 124 27 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 395 404 431 555 945 972 1072
Size: 7 388 9 27 124 390 27 100
Cuts at: 1072 1202 1530 1625 1632
Size: 130 328 95 7
Fragments arranged by size:
390 388 328 130 124 100 95 27
27 9 7 7
BspEI T'CCGG_A
Cuts at: 0 29 308 1476 1632
Size: 29 279 1168 156
Fragments arranged by size:
1168 279 156 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1109 1613 1632
Size: 1109 504 19
Fragments arranged by size:
1109 504 19
BsrBI CCG'CTC
Cuts at: 0 479 1246 1632
Size: 479 767 386
Fragments arranged by size:
767 479 386
BssSI C'ACGA_G
Cuts at: 0 944 1201 1632
Size: 944 257 431
Fragments arranged by size:
944 431 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 1632
Size: 399 1233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 199 1632
Size: 199 1433
Bsu36I CC'TnA_GG
Cuts at: 0 497 506 841 1632
Size: 497 9 335 791
Fragments arranged by size:
791 497 335 9
BtsI GCAGTG_nn'
Cuts at: 0 95 892 921 1632
Size: 95 797 29 711
Fragments arranged by size:
797 711 95 29
DraIII CAC_nnn'GTG
Cuts at: 0 399 1629 1632
Size: 399 1230 3
Fragments arranged by size:
1230 399 3
EaeI y'GGCC_r
Cuts at: 0 116 533 778 1388 1442 1491 1632
Size: 116 417 245 610 54 49 141
Fragments arranged by size:
610 417 245 141 116 54 49
EagI C'GGCC_G
Cuts at: 0 1491 1632
Size: 1491 141
EarI CTCTTCn'nnn_
Cuts at: 0 576 1632
Size: 576 1056
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 700 712 1051 1632
Size: 175 525 12 339 581
Fragments arranged by size:
581 525 339 175 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 496 1615 1632
Size: 325 171 1119 17
Fragments arranged by size:
1119 325 171 17
EcoO109I rG'GnC_Cy
Cuts at: 0 65 502 720 773 1068 1153 1166 1286
Size: 65 437 218 53 295 85 13 120
Cuts at: 1286 1398 1458 1632
Size: 112 60 174
Fragments arranged by size:
437 295 218 174 120 112 85 65
60 53 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 1632
Size: 147 1485
HaeII r_GCGC'y
Cuts at: 0 1309 1632
Size: 1309 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 1189 1221 1632
Size: 26 32 1131 32 411
Fragments arranged by size:
1131 411 32 32 26
KpnI G_GTAC'C
Cuts at: 0 549 1632
Size: 549 1083
MscI TGG'CCA
Cuts at: 0 118 535 1390 1444 1632
Size: 118 417 855 54 188
Fragments arranged by size:
855 417 188 118 54
MslI CAynn'nnrTG
Cuts at: 0 10 1632
Size: 10 1622
MspA1I CmG'CkG
Cuts at: 0 678 768 1049 1128 1632
Size: 678 90 281 79 504
Fragments arranged by size:
678 504 281 90 79
NspI r_CATG'y
Cuts at: 0 603 1632
Size: 603 1029
PasI CC'CwG_GG
Cuts at: 0 483 1291 1530 1632
Size: 483 808 239 102
Fragments arranged by size:
808 483 239 102
PciI A'CATG_T
Cuts at: 0 599 1632
Size: 599 1033
PflMI CCAn_nnn'nTGG
Cuts at: 0 542 678 1632
Size: 542 136 954
Fragments arranged by size:
954 542 136
PfoI T'CCnGG_A
Cuts at: 0 173 191 431 689 1028 1632
Size: 173 18 240 258 339 604
Fragments arranged by size:
604 339 258 240 173 18
PmlI CAC'GTG
Cuts at: 0 339 1632
Size: 339 1293
PpuMI rG'GwC_Cy
Cuts at: 0 720 773 1153 1458 1632
Size: 720 53 380 305 174
Fragments arranged by size:
720 380 305 174 53
PspOMI G'GGCC_C
Cuts at: 0 1068 1632
Size: 1068 564
PstI C_TGCA'G
Cuts at: 0 329 899 1632
Size: 329 570 733
Fragments arranged by size:
733 570 329
PvuII CAG'CTG
Cuts at: 0 678 768 1128 1632
Size: 678 90 360 504
Fragments arranged by size:
678 504 360 90
SanDI GG'GwC_CC
Cuts at: 0 773 1153 1632
Size: 773 380 479
Fragments arranged by size:
773 479 380
SexAI A'CCwGG_T
Cuts at: 0 266 1632
Size: 266 1366
SfcI C'TryA_G
Cuts at: 0 325 895 1632
Size: 325 570 737
Fragments arranged by size:
737 570 325
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1396 1632
Size: 1396 236
SmaI CCC'GGG
Cuts at: 0 967 1632
Size: 967 665
SmlI C'TyrA_G
Cuts at: 0 1056 1632
Size: 1056 576
StuI AGG'CCT
Cuts at: 0 829 1632
Size: 829 803
StyI C'CwwG_G
Cuts at: 0 588 830 1269 1632
Size: 588 242 439 363
Fragments arranged by size:
588 439 363 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 328 360 1632
Size: 90 32 206 32 1272
Fragments arranged by size:
1272 206 90 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 1632
Size: 429 1203
XhoI C'TCGA_G
Cuts at: 0 1056 1632
Size: 1056 576
XmaI C'CCGG_G
Cuts at: 0 965 1632
Size: 965 667
ZraI GAC'GTC
Cuts at: 0 333 1632
Size: 333 1299
Enzymes that do cut:
AarI AatII Acc65I AclI AflIII AleI AlwNI ApaI
ApaLI AvaI AvrII BaeI BanI BanII BbvCI BciVI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BssSI BstAPI BstXI
Bsu36I BtsI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KpnI MscI MslI MspA1I
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SanDI SexAI SfcI SfiI SmaI SmlI
StuI StyI TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AcuI AfeI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI BamHI BbeI BbsI BcgI
BclI BfrBI BglII BlpI BmtI BpuEI BsaBI BsiWI
BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XmnI