Restriction map for
pF1KE0979
(Linear) (Six-base) MAPSORT of: KIEE0979.seq Check: 1012 from: 1 to: 1722
KIEE0979.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 1189 1722
Size: 103 1086 533
Fragments arranged by size:
1086 533 103
AccI GT'mk_AC
Cuts at: 0 584 1722
Size: 584 1138
Acc65I G'GTAC_C
Cuts at: 0 1611 1722
Size: 1611 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 269 298 668 1722
Size: 92 177 29 370 1054
Fragments arranged by size:
1054 370 177 92 29
AfeI AGC'GCT
Cuts at: 0 850 1722
Size: 850 872
AflIII A'CryG_T
Cuts at: 0 1187 1722
Size: 1187 535
AgeI A'CCGG_T
Cuts at: 0 59 1722
Size: 59 1663
AhdI GACnn_n'nnGTC
Cuts at: 0 1191 1722
Size: 1191 531
AlwNI CAG_nnn'CTG
Cuts at: 0 331 1722
Size: 331 1391
ApoI r'AATT_y
Cuts at: 0 99 438 634 791 870 1530 1722
Size: 99 339 196 157 79 660 192
Fragments arranged by size:
660 339 196 192 157 99 79
AvrII C'CTAG_G
Cuts at: 0 1114 1722
Size: 1114 608
BamHI G'GATC_C
Cuts at: 0 1294 1722
Size: 1294 428
BanI G'GyrC_C
Cuts at: 0 560 1611 1722
Size: 560 1051 111
Fragments arranged by size:
1051 560 111
BanII G_rGCy'C
Cuts at: 0 907 1722
Size: 907 815
BbeI G_GCGC'C
Cuts at: 0 564 1722
Size: 564 1158
BbsI GAAGACnn'nnnn_
Cuts at: 0 1480 1722
Size: 1480 242
BclI T'GATC_A
Cuts at: 0 381 1722
Size: 381 1341
BglI GCCn_nnn'nGGC
Cuts at: 0 1117 1722
Size: 1117 605
BglII A'GATC_T
Cuts at: 0 1650 1722
Size: 1650 72
BlpI GC'TnA_GC
Cuts at: 0 653 1722
Size: 653 1069
Bme1580I G_kGCm'C
Cuts at: 0 1165 1722
Size: 1165 557
BmrI ACTGGGnnnn_n'
Cuts at: 0 715 1722
Size: 715 1007
BmtI G_CTAG'C
Cuts at: 0 1043 1722
Size: 1043 679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1496 1722
Size: 430 1066 226
Fragments arranged by size:
1066 430 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1392 1722
Size: 1392 330
BsaBI GATnn'nnATC
Cuts at: 0 1655 1722
Size: 1655 67
BsaHI Gr'CG_yC
Cuts at: 0 7 561 1098 1722
Size: 7 554 537 624
Fragments arranged by size:
624 554 537 7
BsaWI w'CCGG_w
Cuts at: 0 59 1722
Size: 59 1663
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 1722
Size: 403 30 1289
Fragments arranged by size:
1289 403 30
BseYI C'CCAG_C
Cuts at: 0 50 331 538 1035 1722
Size: 50 281 207 497 687
Fragments arranged by size:
687 497 281 207 50
BsiHKAI G_wGCw'C
Cuts at: 0 1143 1722
Size: 1143 579
BsmI GAATG_Cn'
Cuts at: 0 500 1722
Size: 500 1222
Bsp1286I G_dGCh'C
Cuts at: 0 907 1143 1165 1722
Size: 907 236 22 557
Fragments arranged by size:
907 557 236 22
BspHI T'CATG_A
Cuts at: 0 920 1722
Size: 920 802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 1095 1111 1189 1640 1722
Size: 103 992 16 78 451 82
Fragments arranged by size:
992 451 103 82 78 16
BsrFI r'CCGG_y
Cuts at: 0 59 1722
Size: 59 1663
BstYI r'GATC_y
Cuts at: 0 826 1294 1650 1722
Size: 826 468 356 72
Fragments arranged by size:
826 468 356 72
Bsu36I CC'TnA_GG
Cuts at: 0 728 1722
Size: 728 994
BtsI GCAGTG_nn'
Cuts at: 0 917 1722
Size: 917 805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 920 955 1722
Size: 920 35 767
Fragments arranged by size:
920 767 35
DraI TTT'AAA
Cuts at: 0 83 105 1539 1707 1722
Size: 83 22 1434 168 15
Fragments arranged by size:
1434 168 83 22 15
DraIII CAC_nnn'GTG
Cuts at: 0 715 1722
Size: 715 1007
EaeI y'GGCC_r
Cuts at: 0 221 1722
Size: 221 1501
EarI CTCTTCn'nnn_
Cuts at: 0 245 1722
Size: 245 1477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 269 298 430 668 1496 1722
Size: 92 177 29 132 238 828 226
Fragments arranged by size:
828 238 226 177 132 92 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1118 1722
Size: 1118 604
EcoO109I rG'GnC_Cy
Cuts at: 0 244 1722
Size: 244 1478
EcoRI G'AATT_C
Cuts at: 0 438 1530 1722
Size: 438 1092 192
Fragments arranged by size:
1092 438 192
EcoRV GAT'ATC
Cuts at: 0 1234 1722
Size: 1234 488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 594 1722
Size: 594 1128
HaeII r_GCGC'y
Cuts at: 0 564 852 1722
Size: 564 288 870
Fragments arranged by size:
870 564 288
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 730 762 934 966 1177 1209
Size: 403 32 295 32 172 32 211 32
Cuts at: 1209 1292 1324 1722
Size: 83 32 398
Fragments arranged by size:
403 398 295 211 172 83 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1424 1722
Size: 1424 298
HindIII A'AGCT_T
Cuts at: 0 136 488 758 1487 1622 1685 1722
Size: 136 352 270 729 135 63 37
Fragments arranged by size:
729 352 270 136 135 63 37
HpaI GTT'AAC
Cuts at: 0 1424 1722
Size: 1424 298
KasI G'GCGC_C
Cuts at: 0 560 1722
Size: 560 1162
KpnI G_GTAC'C
Cuts at: 0 1615 1722
Size: 1615 107
MfeI C'AATT_G
Cuts at: 0 1181 1722
Size: 1181 541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1722
Size: 304 1418
MscI TGG'CCA
Cuts at: 0 223 1722
Size: 223 1499
MslI CAynn'nnrTG
Cuts at: 0 527 1722
Size: 527 1195
MspA1I CmG'CkG
Cuts at: 0 307 1722
Size: 307 1415
NarI GG'CG_CC
Cuts at: 0 561 1722
Size: 561 1161
NheI G'CTAG_C
Cuts at: 0 1039 1722
Size: 1039 683
NspI r_CATG'y
Cuts at: 0 1191 1447 1722
Size: 1191 256 275
Fragments arranged by size:
1191 275 256
PciI A'CATG_T
Cuts at: 0 1187 1722
Size: 1187 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 715 1722
Size: 715 1007
PfoI T'CCnGG_A
Cuts at: 0 1512 1722
Size: 1512 210
PpuMI rG'GwC_Cy
Cuts at: 0 244 1722
Size: 244 1478
PsiI TTA'TAA
Cuts at: 0 1244 1722
Size: 1244 478
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 597 629 1722
Size: 597 32 1093
Fragments arranged by size:
1093 597 32
PstI C_TGCA'G
Cuts at: 0 912 1200 1722
Size: 912 288 522
Fragments arranged by size:
912 522 288
PvuII CAG'CTG
Cuts at: 0 307 1722
Size: 307 1415
SfcI C'TryA_G
Cuts at: 0 830 908 1175 1196 1722
Size: 830 78 267 21 526
Fragments arranged by size:
830 526 267 78 21
SfoI GGC'GCC
Cuts at: 0 562 1722
Size: 562 1160
SmlI C'TyrA_G
Cuts at: 0 1371 1722
Size: 1371 351
SphI G_CATG'C
Cuts at: 0 1447 1722
Size: 1447 275
SspI AAT'ATT
Cuts at: 0 1340 1722
Size: 1340 382
StuI AGG'CCT
Cuts at: 0 836 1722
Size: 836 886
StyI C'CwwG_G
Cuts at: 0 578 600 1114 1722
Size: 578 22 514 608
Fragments arranged by size:
608 578 514 22
TatI w'GTAC_w
Cuts at: 0 945 1722
Size: 945 777
XbaI T'CTAG_A
Cuts at: 0 952 1647 1722
Size: 952 695 75
Fragments arranged by size:
952 695 75
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AlwNI ApoI AvrII BamHI BanI BanII BbeI BbsI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BpmI
BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BstYI Bsu36I BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MmeI MscI MslI MspA1I NarI NheI
NspI PciI PflMI PfoI PpuMI PsiI PsrI PstI
PvuII SfcI SfoI SmlI SphI SspI StuI StyI
TatI XbaI
Enzymes that do not cut:
AatII AclI AflII AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvaI BaeI BbvCI BcgI BciVI
BfrBI BmgBI BplI Bpu10I BsaI BsaAI BseRI BsgI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
ClaI DrdI EagI EciI EcoICRI FseI FspI FspAI
MluI NaeI NcoI NdeI NgoMIV NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TaqII TstI Tth111I XcmI XhoI XmaI
XmnI ZraI