Restriction map for
pF1KE0989
(Linear) (Six-base) MAPSORT of: KIEE0989.seq Check: 1884 from: 1 to: 1869
KIEE0989.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 506 524 1697 1869
Size: 181 325 18 1173 172
Fragments arranged by size:
1173 325 181 172 18
AlwNI CAG_nnn'CTG
Cuts at: 0 1029 1056 1869
Size: 1029 27 813
Fragments arranged by size:
1029 813 27
ApaLI G'TGCA_C
Cuts at: 0 1805 1825 1869
Size: 1805 20 44
Fragments arranged by size:
1805 44 20
ApoI r'AATT_y
Cuts at: 0 632 1869
Size: 632 1237
AseI AT'TA_AT
Cuts at: 0 265 1869
Size: 265 1604
AvaI C'yCGr_G
Cuts at: 0 1116 1869
Size: 1116 753
BamHI G'GATC_C
Cuts at: 0 801 1682 1869
Size: 801 881 187
Fragments arranged by size:
881 801 187
BciVI GTATCCnnnnn_n'
Cuts at: 0 673 1869
Size: 673 1196
BfrBI ATG'CAT
Cuts at: 0 134 1869
Size: 134 1735
BglII A'GATC_T
Cuts at: 0 517 1869
Size: 517 1352
Bme1580I G_kGCm'C
Cuts at: 0 501 1809 1829 1869
Size: 501 1308 20 40
Fragments arranged by size:
1308 501 40 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 711 1561 1851 1869
Size: 711 850 290 18
Fragments arranged by size:
850 711 290 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1869
Size: 1229 640
Bpu10I CC'TnA_GC
Cuts at: 0 962 1057 1869
Size: 962 95 812
Fragments arranged by size:
962 812 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1869
Size: 162 1707
BsaAI yAC'GTr
Cuts at: 0 41 1869
Size: 41 1828
BseYI C'CCAG_C
Cuts at: 0 1004 1217 1869
Size: 1004 213 652
Fragments arranged by size:
1004 652 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1684 1869
Size: 1684 185
BsiEI CG_ry'CG
Cuts at: 0 817 1869
Size: 817 1052
BsiHKAI G_wGCw'C
Cuts at: 0 1809 1829 1869
Size: 1809 20 40
Fragments arranged by size:
1809 40 20
BsmI GAATG_Cn'
Cuts at: 0 136 1869
Size: 136 1733
Bsp1286I G_dGCh'C
Cuts at: 0 501 1809 1829 1869
Size: 501 1308 20 40
Fragments arranged by size:
1308 501 40 20
BspHI T'CATG_A
Cuts at: 0 779 1869
Size: 779 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1383 1869
Size: 1383 486
BsrDI GCAATG_nn'
Cuts at: 0 1596 1869
Size: 1596 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1573 1869
Size: 1029 544 296
Fragments arranged by size:
1029 544 296
BstXI CCAn_nnnn'nTGG
Cuts at: 0 355 1551 1869
Size: 355 1196 318
Fragments arranged by size:
1196 355 318
BstYI r'GATC_y
Cuts at: 0 517 801 1682 1869
Size: 517 284 881 187
Fragments arranged by size:
881 517 284 187
Bsu36I CC'TnA_GG
Cuts at: 0 1335 1869
Size: 1335 534
BtsI GCAGTG_nn'
Cuts at: 0 1027 1869
Size: 1027 842
ClaI AT'CG_AT
Cuts at: 0 983 1869
Size: 983 886
DraI TTT'AAA
Cuts at: 0 630 908 1284 1869
Size: 630 278 376 585
Fragments arranged by size:
630 585 376 278
EaeI y'GGCC_r
Cuts at: 0 89 670 1674 1847 1869
Size: 89 581 1004 173 22
Fragments arranged by size:
1004 581 173 89 22
EarI CTCTTCn'nnn_
Cuts at: 0 100 1217 1869
Size: 100 1117 652
Fragments arranged by size:
1117 652 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 506 524 1229 1697 1869
Size: 181 325 18 705 468 172
Fragments arranged by size:
705 468 325 181 172 18
EcoO109I rG'GnC_Cy
Cuts at: 0 871 1555 1869
Size: 871 684 314
Fragments arranged by size:
871 684 314
EcoRV GAT'ATC
Cuts at: 0 1456 1869
Size: 1456 413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1503 1869
Size: 1503 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1869
Size: 463 32 1374
Fragments arranged by size:
1374 463 32
HincII GTy'rAC
Cuts at: 0 1412 1869
Size: 1412 457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 975 1208 1367 1444 1869
Size: 537 438 233 159 77 425
Fragments arranged by size:
537 438 425 233 159 77
MscI TGG'CCA
Cuts at: 0 91 672 1676 1849 1869
Size: 91 581 1004 173 20
Fragments arranged by size:
1004 581 173 91 20
MslI CAynn'nnrTG
Cuts at: 0 16 179 353 544 1169 1869
Size: 16 163 174 191 625 700
Fragments arranged by size:
700 625 191 174 163 16
MspA1I CmG'CkG
Cuts at: 0 687 918 1672 1869
Size: 687 231 754 197
Fragments arranged by size:
754 687 231 197
NsiI A_TGCA'T
Cuts at: 0 136 1869
Size: 136 1733
NspI r_CATG'y
Cuts at: 0 1137 1869
Size: 1137 732
PasI CC'CwG_GG
Cuts at: 0 601 1869
Size: 601 1268
PpuMI rG'GwC_Cy
Cuts at: 0 1555 1869
Size: 1555 314
PsiI TTA'TAA
Cuts at: 0 1769 1869
Size: 1769 100
PstI C_TGCA'G
Cuts at: 0 1034 1869
Size: 1034 835
PvuII CAG'CTG
Cuts at: 0 687 918 1672 1869
Size: 687 231 754 197
Fragments arranged by size:
754 687 231 197
SanDI GG'GwC_CC
Cuts at: 0 1555 1869
Size: 1555 314
ScaI AGT'ACT
Cuts at: 0 1435 1869
Size: 1435 434
SfcI C'TryA_G
Cuts at: 0 1030 1775 1869
Size: 1030 745 94
Fragments arranged by size:
1030 745 94
SmlI C'TyrA_G
Cuts at: 0 141 1869
Size: 141 1728
SspI AAT'ATT
Cuts at: 0 312 326 1794 1869
Size: 312 14 1468 75
Fragments arranged by size:
1468 312 75 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 799 831 857 1264 1833 1869
Size: 799 32 26 407 569 36
Fragments arranged by size:
799 569 407 36 32 26
TatI w'GTAC_w
Cuts at: 0 1433 1869
Size: 1433 436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 1869
Size: 24 1845
XmnI GAAnn'nnTTC
Cuts at: 0 770 1869
Size: 770 1099
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI AseI AvaI BamHI BciVI
BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BstAPI BstXI BstYI Bsu36I BtsI ClaI DraI
EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
MmeI MscI MslI MspA1I NsiI NspI PasI PpuMI
PsiI PstI PvuII SanDI ScaI SfcI SmlI SspI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AsiSI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI BmgBI BmtI BplI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BsiWI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI
BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI SwaI TstI Tth111I XbaI XhoI XmaI ZraI