Restriction map for
pF1KE0993
(Linear) (Six-base) MAPSORT of: KIEE0993.seq Check: 9774 from: 1 to: 1935
KIEE0993.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 986 1935
Size: 986 949
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1445 1667 1935
Size: 1445 222 268
Fragments arranged by size:
1445 268 222
AflII C'TTAA_G
Cuts at: 0 178 1935
Size: 178 1757
AflIII A'CryG_T
Cuts at: 0 1652 1935
Size: 1652 283
AhdI GACnn_n'nnGTC
Cuts at: 0 477 633 645 1935
Size: 477 156 12 1290
Fragments arranged by size:
1290 477 156 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1138 1808 1935
Size: 1138 670 127
Fragments arranged by size:
1138 670 127
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1904 1935
Size: 1904 31
AlwNI CAG_nnn'CTG
Cuts at: 0 426 987 1155 1734 1850 1872 1893 1935
Size: 426 561 168 579 116 22 21 42
Fragments arranged by size:
579 561 426 168 116 42 22 21
ApoI r'AATT_y
Cuts at: 0 7 356 839 918 1935
Size: 7 349 483 79 1017
Fragments arranged by size:
1017 483 349 79 7
AseI AT'TA_AT
Cuts at: 0 953 1935
Size: 953 982
BamHI G'GATC_C
Cuts at: 0 550 971 1528 1935
Size: 550 421 557 407
Fragments arranged by size:
557 550 421 407
BanI G'GyrC_C
Cuts at: 0 1833 1935
Size: 1833 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1233 1415 1655 1935
Size: 917 316 182 240 280
Fragments arranged by size:
917 316 280 240 182
BbvCI CC'TCA_GC
Cuts at: 0 1039 1935
Size: 1039 896
BciVI GTATCCnnnnn_n'
Cuts at: 0 452 1384 1935
Size: 452 932 551
Fragments arranged by size:
932 551 452
BclI T'GATC_A
Cuts at: 0 1895 1935
Size: 1895 40
BfrBI ATG'CAT
Cuts at: 0 447 659 691 1444 1935
Size: 447 212 32 753 491
Fragments arranged by size:
753 491 447 212 32
BglII A'GATC_T
Cuts at: 0 342 1135 1935
Size: 342 793 800
Fragments arranged by size:
800 793 342
Bme1580I G_kGCm'C
Cuts at: 0 996 1935
Size: 996 939
BmtI G_CTAG'C
Cuts at: 0 1799 1935
Size: 1799 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1906 1935
Size: 1033 873 29
Fragments arranged by size:
1033 873 29
Bpu10I CC'TnA_GC
Cuts at: 0 1039 1935
Size: 1039 896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1756 1823 1935
Size: 1756 67 112
Fragments arranged by size:
1756 112 67
BsaAI yAC'GTr
Cuts at: 0 87 1935
Size: 87 1848
BsaHI Gr'CG_yC
Cuts at: 0 1018 1935
Size: 1018 917
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 567 597 737 767 1904 1934 1935
Size: 567 30 140 30 1137 30 1
Fragments arranged by size:
1137 567 140 30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 173 235 468 1225 1489 1935
Size: 173 62 233 757 264 446
Fragments arranged by size:
757 446 264 233 173 62
BseYI C'CCAG_C
Cuts at: 0 264 429 1532 1734 1935
Size: 264 165 1103 202 201
Fragments arranged by size:
1103 264 202 201 165
BsmI GAATG_Cn'
Cuts at: 0 691 1127 1797 1935
Size: 691 436 670 138
Fragments arranged by size:
691 670 436 138
Bsp1286I G_dGCh'C
Cuts at: 0 996 1935
Size: 996 939
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 986 1319 1935
Size: 986 333 616
Fragments arranged by size:
986 616 333
BsrGI T'GTAC_A
Cuts at: 0 219 1935
Size: 219 1716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1590 1935
Size: 1590 345
BstYI r'GATC_y
Cuts at: 0 342 550 971 1135 1400 1528 1808 1935
Size: 342 208 421 164 265 128 280 127
Fragments arranged by size:
421 342 280 265 208 164 128 127
Bsu36I CC'TnA_GG
Cuts at: 0 602 1220 1935
Size: 602 618 715
Fragments arranged by size:
715 618 602
BtgI C'CryG_G
Cuts at: 0 1337 1640 1935
Size: 1337 303 295
Fragments arranged by size:
1337 303 295
BtsI GCAGTG_nn'
Cuts at: 0 114 1935
Size: 114 1821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 275 310 1935
Size: 275 35 1625
Fragments arranged by size:
1625 275 35
DraI TTT'AAA
Cuts at: 0 725 845 1190 1935
Size: 725 120 345 745
Fragments arranged by size:
745 725 345 120
EaeI y'GGCC_r
Cuts at: 0 563 1580 1688 1935
Size: 563 1017 108 247
Fragments arranged by size:
1017 563 247 108
EarI CTCTTCn'nnn_
Cuts at: 0 1253 1274 1421 1457 1748 1877 1935
Size: 1253 21 147 36 291 129 58
Fragments arranged by size:
1253 291 147 129 58 36 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1445 1667 1906 1935
Size: 1033 412 222 239 29
Fragments arranged by size:
1033 412 239 222 29
EcoRV GAT'ATC
Cuts at: 0 766 1569 1935
Size: 766 803 366
Fragments arranged by size:
803 766 366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1250 1432 1935
Size: 1250 182 503
Fragments arranged by size:
1250 503 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 450 463 482 495 619 631 651 663
Size: 450 13 19 13 124 12 20 12
Cuts at: 663 1363 1395 1935
Size: 700 32 540
Fragments arranged by size:
700 540 450 124 32 20 19 13
13 12 12
HincII GTy'rAC
Cuts at: 0 438 1935
Size: 438 1497
MfeI C'AATT_G
Cuts at: 0 1361 1935
Size: 1361 574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 617 1935
Size: 174 443 1318
Fragments arranged by size:
1318 443 174
MscI TGG'CCA
Cuts at: 0 565 1582 1690 1935
Size: 565 1017 108 245
Fragments arranged by size:
1017 565 245 108
MslI CAynn'nnrTG
Cuts at: 0 654 1639 1790 1935
Size: 654 985 151 145
Fragments arranged by size:
985 654 151 145
MspA1I CmG'CkG
Cuts at: 0 423 1155 1935
Size: 423 732 780
Fragments arranged by size:
780 732 423
NcoI C'CATG_G
Cuts at: 0 1337 1640 1935
Size: 1337 303 295
Fragments arranged by size:
1337 303 295
NheI G'CTAG_C
Cuts at: 0 1795 1935
Size: 1795 140
NsiI A_TGCA'T
Cuts at: 0 449 661 693 1446 1935
Size: 449 212 32 753 489
Fragments arranged by size:
753 489 449 212 32
NspI r_CATG'y
Cuts at: 0 447 659 801 1656 1935
Size: 447 212 142 855 279
Fragments arranged by size:
855 447 279 212 142
PciI A'CATG_T
Cuts at: 0 1652 1935
Size: 1652 283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1904 1935
Size: 1904 31
PstI C_TGCA'G
Cuts at: 0 983 1935
Size: 983 952
PvuII CAG'CTG
Cuts at: 0 423 1155 1935
Size: 423 732 780
Fragments arranged by size:
780 732 423
SapI GCTCTTCn'nnn_
Cuts at: 0 1877 1935
Size: 1877 58
ScaI AGT'ACT
Cuts at: 0 328 1432 1935
Size: 328 1104 503
Fragments arranged by size:
1104 503 328
SfcI C'TryA_G
Cuts at: 0 871 979 1935
Size: 871 108 956
Fragments arranged by size:
956 871 108
SmlI C'TyrA_G
Cuts at: 0 178 1771 1838 1935
Size: 178 1593 67 97
Fragments arranged by size:
1593 178 97 67
SphI G_CATG'C
Cuts at: 0 659 1935
Size: 659 1276
StyI C'CwwG_G
Cuts at: 0 618 1337 1386 1640 1935
Size: 618 719 49 254 295
Fragments arranged by size:
719 618 295 254 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 1935
Size: 278 1657
TatI w'GTAC_w
Cuts at: 0 219 326 1005 1167 1430 1874 1935
Size: 219 107 679 162 263 444 61
Fragments arranged by size:
679 444 263 219 162 107 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 737 769 1935
Size: 737 32 1166
Fragments arranged by size:
1166 737 32
XbaI T'CTAG_A
Cuts at: 0 1617 1935
Size: 1617 318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1638 1731 1935
Size: 1638 93 204
Fragments arranged by size:
1638 204 93
XmnI GAAnn'nnTTC
Cuts at: 0 1230 1935
Size: 1230 705
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI AlfI AloI AlwNI
ApoI AseI BamHI BanI BbsI BbvCI BciVI BclI
BfrBI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsmI Bsp1286I BspMI BsrGI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI
EarI Eco57MI EcoRV FalI Hin4I HincII MfeI MmeI
MscI MslI MspA1I NcoI NheI NsiI NspI PciI
PpiI PstI PvuII SapI ScaI SfcI SmlI SphI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AleI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BanII BbeI
BcgI BglI BlpI BmgBI BmrI BplI BsaI BsaBI
BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XhoI XmaI ZraI