Restriction map for pF1KE0996
 (Linear) (Six-base) MAPSORT of: KIEE0996.seq  Check: 9895  from: 1  to: 1977



KIEE0996.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     371     830    1334    1850    1977 
   Size:         52     319     459     504     516     127

  Fragments arranged by size: 

                 516     504     459     319     127      52
 
AhdI GACnn_n'nnGTC

Cuts at:      0     863    1977 
   Size:        863    1114
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1112    1977 
   Size:       1112     865
 
AlwNI CAG_nnn'CTG

Cuts at:      0     952    1491    1977 
   Size:        952     539     486

  Fragments arranged by size: 

                 952     539     486
 
ApaLI G'TGCA_C

Cuts at:      0     100    1977 
   Size:        100    1877
 
ApoI r'AATT_y

Cuts at:      0     272     497    1321    1672    1766    1934    1977 
   Size:        272     225     824     351      94     168      43

  Fragments arranged by size: 

                 824     351     272     225     168      94      43
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     390     423    1977 
   Size:        390      33    1554

  Fragments arranged by size: 

                1554     390      33
 
BanII G_rGCy'C

Cuts at:      0    1073    1977 
   Size:       1073     904
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     330     553     824    1977 
   Size:        330     223     271    1153

  Fragments arranged by size: 

                1153     330     271     223
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1165    1977 
   Size:       1165     812
 
BclI T'GATC_A

Cuts at:      0    1292    1977 
   Size:       1292     685
 
BglII A'GATC_T

Cuts at:      0     587    1977 
   Size:        587    1390
 
BlpI GC'TnA_GC

Cuts at:      0     872    1977 
   Size:        872    1105
 
Bme1580I G_kGCm'C

Cuts at:      0     104    1977 
   Size:        104    1873
 
BmgBI CAC'GTC

Cuts at:      0    1278    1977 
   Size:       1278     699
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     206     909    1555    1977 
   Size:         37     169     703     646     422

  Fragments arranged by size: 

                 703     646     422     169      37
 
Bpu10I CC'TnA_GC

Cuts at:      0     611    1067    1977 
   Size:        611     456     910

  Fragments arranged by size: 

                 910     611     456
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     965    1977 
   Size:        965    1012
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1553    1977 
   Size:       1553     424
 
BsaBI GATnn'nnATC

Cuts at:      0    1176    1977 
   Size:       1176     801
 
BsaHI Gr'CG_yC

Cuts at:      0    1385    1977 
   Size:       1385     592
 
BsaWI w'CCGG_w

Cuts at:      0     395     542    1682    1695    1977 
   Size:        395     147    1140      13     282

  Fragments arranged by size: 

                1140     395     282     147      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      94     124     325     355    1977 
   Size:         94      30     201      30    1622

  Fragments arranged by size: 

                1622     201      94      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     209     592    1977 
   Size:        209     383    1385

  Fragments arranged by size: 

                1385     383     209
 
BseYI C'CCAG_C

Cuts at:      0    1221    1977 
   Size:       1221     756
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1374    1393    1977 
   Size:       1374      19     584

  Fragments arranged by size: 

                1374     584      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     104     762    1073    1977 
   Size:        104     658     311     904

  Fragments arranged by size: 

                 904     658     311     104
 
BsmI GAATG_Cn'

Cuts at:      0     690    1152    1977 
   Size:        690     462     825

  Fragments arranged by size: 

                 825     690     462
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1849    1977 
   Size:       1849     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     104     762    1073    1977 
   Size:        104     658     311     904

  Fragments arranged by size: 

                 904     658     311     104
 
BspHI T'CATG_A

Cuts at:      0    1343    1977 
   Size:       1343     634
 
BsrDI GCAATG_nn'

Cuts at:      0    1826    1977 
   Size:       1826     151
 
BsrGI T'GTAC_A

Cuts at:      0    1889    1977 
   Size:       1889      88
 
BssSI C'ACGA_G

Cuts at:      0     761    1977 
   Size:        761    1216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264    1685    1977 
   Size:        264    1421     292

  Fragments arranged by size: 

                1421     292     264
 
BstYI r'GATC_y

Cuts at:      0     587    1977 
   Size:        587    1390
 
Bsu36I CC'TnA_GG

Cuts at:      0     575    1456    1977 
   Size:        575     881     521

  Fragments arranged by size: 

                 881     575     521
 
BtgI C'CryG_G

Cuts at:      0     748    1523    1977 
   Size:        748     775     454

  Fragments arranged by size: 

                 775     748     454
 
BtsI GCAGTG_nn'

Cuts at:      0      14    1157    1977 
   Size:         14    1143     820

  Fragments arranged by size: 

                1143     820      14
 
ClaI AT'CG_AT

Cuts at:      0     443    1977 
   Size:        443    1534
 
EaeI y'GGCC_r

Cuts at:      0       2    1280    1430    1977 
   Size:          2    1278     150     547

  Fragments arranged by size: 

                1278     547     150       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     138     237     245     566    1977 
   Size:        138      99       8     321    1411

  Fragments arranged by size: 

                1411     321     138      99       8
 
EcoICRI GAG'CTC

Cuts at:      0    1071    1977 
   Size:       1071     906
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37      52     206     371     830     909    1334    1555 
   Size:         37      15     154     165     459      79     425     221

Cuts at:   1555    1850    1977 
   Size:        295     127

  Fragments arranged by size: 

                 459     425     295     221     165     154     127      79
                  37      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     325     975    1200    1977 
   Size:        325     650     225     777

  Fragments arranged by size: 

                 777     650     325     225
 
EcoRI G'AATT_C

Cuts at:      0    1321    1977 
   Size:       1321     656
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     311    1977 
   Size:        311    1666
 
HaeII r_GCGC'y

Cuts at:      0      84    1404    1977 
   Size:         84    1320     573

  Fragments arranged by size: 

                1320     573      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     849     881    1957    1977 
   Size:        849      32    1076      20

  Fragments arranged by size: 

                1076     849      32      20
 
HindIII A'AGCT_T

Cuts at:      0     519    1977 
   Size:        519    1458
 
MscI TGG'CCA

Cuts at:      0    1282    1432    1977 
   Size:       1282     150     545

  Fragments arranged by size: 

                1282     545     150
 
MslI CAynn'nnrTG

Cuts at:      0     679     829    1288    1977 
   Size:        679     150     459     689

  Fragments arranged by size: 

                 689     679     459     150
 
MspA1I CmG'CkG

Cuts at:      0     702    1491    1977 
   Size:        702     789     486

  Fragments arranged by size: 

                 789     702     486
 
NcoI C'CATG_G

Cuts at:      0     748    1523    1977 
   Size:        748     775     454

  Fragments arranged by size: 

                 775     748     454
 
NdeI CA'TA_TG

Cuts at:      0    1791    1977 
   Size:       1791     186
 
NspI r_CATG'y

Cuts at:      0     678    1977 
   Size:        678    1299
 
PasI CC'CwG_GG

Cuts at:      0    1776    1977 
   Size:       1776     201
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     501    1977 
   Size:        501    1476
 
PfoI T'CCnGG_A

Cuts at:      0    1637    1977 
   Size:       1637     340
 
PpuMI rG'GwC_Cy

Cuts at:      0     975    1200    1977 
   Size:        975     225     777

  Fragments arranged by size: 

                 975     777     225
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     144     176    1702    1734    1977 
   Size:        144      32    1526      32     243

  Fragments arranged by size: 

                1526     243     144      32      32
 
PstI C_TGCA'G

Cuts at:      0    1101    1164    1977 
   Size:       1101      63     813

  Fragments arranged by size: 

                1101     813      63
 
PvuII CAG'CTG

Cuts at:      0     702    1491    1977 
   Size:        702     789     486

  Fragments arranged by size: 

                 789     702     486
 
SacI G_AGCT'C

Cuts at:      0    1073    1977 
   Size:       1073     904
 
SanDI GG'GwC_CC

Cuts at:      0     975    1977 
   Size:        975    1002
 
SfcI C'TryA_G

Cuts at:      0     697    1097    1160    1848    1977 
   Size:        697     400      63     688     129

  Fragments arranged by size: 

                 697     688     400     129      63
 
SmlI C'TyrA_G

Cuts at:      0     980    1977 
   Size:        980     997
 
SspI AAT'ATT

Cuts at:      0     721    1950    1977 
   Size:        721    1229      27

  Fragments arranged by size: 

                1229     721      27
 
StuI AGG'CCT

Cuts at:      0    1166    1838    1854    1977 
   Size:       1166     672      16     123

  Fragments arranged by size: 

                1166     672     123      16
 
StyI C'CwwG_G

Cuts at:      0     748    1523    1833    1977 
   Size:        748     775     310     144

  Fragments arranged by size: 

                 775     748     310     144
 
TatI w'GTAC_w

Cuts at:      0    1424    1889    1977 
   Size:       1424     465      88

  Fragments arranged by size: 

                1424     465      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      94     126    1685    1717    1977 
   Size:         94      32    1559      32     260

  Fragments arranged by size: 

                1559     260      94      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1953    1977 
   Size:       1953      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483    1164    1530    1635    1977 
   Size:        483     681     366     105     342

  Fragments arranged by size: 

                 681     483     366     342     105
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI    ApaLI     ApoI     BaeI    BanII
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BsrDI
    BsrGI    BssSI    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I  HindIII     MscI     MslI   MspA1I     NcoI     NdeI     NspI
     PasI    PflMI     PfoI    PpuMI     PsrI     PstI    PvuII     SacI
    SanDI     SfcI     SmlI     SspI     StuI     StyI     TatI     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI
     BmrI     BmtI     BplI    BsaAI    BsiEI    BsiWI    BspEI    BspMI
    BsrBI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII  Tth111I     XhoI     XmaI
     XmnI     ZraI
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