(Linear) (Six-base) MAPSORT of: KIEE1002.seq  Check: 9940  from: 1  to: 447



KIEE1002.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     447 
   Size:         38     409
 
ApoI r'AATT_y

Cuts at:      0      34     202     447 
   Size:         34     168     245

  Fragments arranged by size: 

                 245     168      34
 
AseI AT'TA_AT

Cuts at:      0     158     447 
   Size:        158     289
 
BclI T'GATC_A

Cuts at:      0     374     447 
   Size:        374      73
 
BglII A'GATC_T

Cuts at:      0     112     447 
   Size:        112     335
 
BsmI GAATG_Cn'

Cuts at:      0     265     447 
   Size:        265     182
 
BsrDI GCAATG_nn'

Cuts at:      0     297     447 
   Size:        297     150
 
BsrGI T'GTAC_A

Cuts at:      0     426     447 
   Size:        426      21
 
BstYI r'GATC_y

Cuts at:      0     112     447 
   Size:        112     335
 
EarI CTCTTCn'nnn_

Cuts at:      0      14     413     447 
   Size:         14     399      34

  Fragments arranged by size: 

                 399      34      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     447 
   Size:         38     409
 
EcoRI G'AATT_C

Cuts at:      0      34     447 
   Size:         34     413
 
HincII GTy'rAC

Cuts at:      0     350     447 
   Size:        350      97
 
HindIII A'AGCT_T

Cuts at:      0      44     447 
   Size:         44     403
 
HpaI GTT'AAC

Cuts at:      0     350     447 
   Size:        350      97
 
MfeI C'AATT_G

Cuts at:      0     188     447 
   Size:        188     259
 
PstI C_TGCA'G

Cuts at:      0     312     447 
   Size:        312     135
 
SapI GCTCTTCn'nnn_

Cuts at:      0      14     447 
   Size:         14     433
 
SfcI C'TryA_G

Cuts at:      0     308     447 
   Size:        308     139
 
StyI C'CwwG_G

Cuts at:      0     336     447 
   Size:        336     111
 
TatI w'GTAC_w

Cuts at:      0     426     447 
   Size:        426      21
 

 Enzymes that do cut:

     AcuI     ApoI     AseI     BclI    BglII     BsmI    BsrDI    BsrGI
    BstYI     EarI  Eco57MI    EcoRI   HincII  HindIII     HpaI     MfeI
     PstI     SapI     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI