Restriction map for
pF1KE1005
(Linear) (Six-base) MAPSORT of: KIEE1005.seq Check: 8902 from: 1 to: 558
KIEE1005.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 355 558
Size: 355 203
AccI GT'mk_AC
Cuts at: 0 227 558
Size: 227 331
ApoI r'AATT_y
Cuts at: 0 496 558
Size: 496 62
AvaI C'yCGr_G
Cuts at: 0 26 199 394 558
Size: 26 173 195 164
Fragments arranged by size:
195 173 164 26
BamHI G'GATC_C
Cuts at: 0 245 558
Size: 245 313
BanI G'GyrC_C
Cuts at: 0 70 558
Size: 70 488
BbsI GAAGACnn'nnnn_
Cuts at: 0 374 413 558
Size: 374 39 145
Fragments arranged by size:
374 145 39
BfrBI ATG'CAT
Cuts at: 0 3 558
Size: 3 555
BglI GCCn_nnn'nGGC
Cuts at: 0 22 334 558
Size: 22 312 224
Fragments arranged by size:
312 224 22
Bme1580I G_kGCm'C
Cuts at: 0 73 97 558
Size: 73 24 461
Fragments arranged by size:
461 73 24
BmgBI CAC'GTC
Cuts at: 0 207 558
Size: 207 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 244 558
Size: 244 314
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 558
Size: 421 137
Bpu10I CC'TnA_GC
Cuts at: 0 234 558
Size: 234 324
BsaAI yAC'GTr
Cuts at: 0 531 558
Size: 531 27
BsaWI w'CCGG_w
Cuts at: 0 477 558
Size: 477 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 380 412 424 558
Size: 187 193 32 12 134
Fragments arranged by size:
193 187 134 32 12
BseYI C'CCAG_C
Cuts at: 0 123 290 453 558
Size: 123 167 163 105
Fragments arranged by size:
167 163 123 105
Bsp1286I G_dGCh'C
Cuts at: 0 73 97 558
Size: 73 24 461
Fragments arranged by size:
461 73 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 558
Size: 355 203
BsrBI CCG'CTC
Cuts at: 0 195 233 493 558
Size: 195 38 260 65
Fragments arranged by size:
260 195 65 38
BstYI r'GATC_y
Cuts at: 0 245 378 558
Size: 245 133 180
Fragments arranged by size:
245 180 133
BtgI C'CryG_G
Cuts at: 0 20 297 558
Size: 20 277 261
Fragments arranged by size:
277 261 20
DraIII CAC_nnn'GTG
Cuts at: 0 77 303 558
Size: 77 226 255
Fragments arranged by size:
255 226 77
EaeI y'GGCC_r
Cuts at: 0 11 537 558
Size: 11 526 21
Fragments arranged by size:
526 21 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 558
Size: 421 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 558
Size: 436 122
EcoO109I rG'GnC_Cy
Cuts at: 0 317 558
Size: 317 241
EcoRI G'AATT_C
Cuts at: 0 496 558
Size: 496 62
HaeII r_GCGC'y
Cuts at: 0 124 297 558
Size: 124 173 261
Fragments arranged by size:
261 173 124
MscI TGG'CCA
Cuts at: 0 539 558
Size: 539 19
MslI CAynn'nnrTG
Cuts at: 0 356 558
Size: 356 202
MspA1I CmG'CkG
Cuts at: 0 243 558
Size: 243 315
NcoI C'CATG_G
Cuts at: 0 20 558
Size: 20 538
NsiI A_TGCA'T
Cuts at: 0 5 558
Size: 5 553
NspI r_CATG'y
Cuts at: 0 7 417 558
Size: 7 410 141
Fragments arranged by size:
410 141 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 256 303 558
Size: 256 47 255
Fragments arranged by size:
256 255 47
PfoI T'CCnGG_A
Cuts at: 0 437 558
Size: 437 121
PstI C_TGCA'G
Cuts at: 0 59 558
Size: 59 499
SexAI A'CCwGG_T
Cuts at: 0 73 558
Size: 73 485
SfcI C'TryA_G
Cuts at: 0 55 558
Size: 55 503
SmaI CCC'GGG
Cuts at: 0 28 201 558
Size: 28 173 357
Fragments arranged by size:
357 173 28
StyI C'CwwG_G
Cuts at: 0 20 558
Size: 20 538
XmaI C'CCGG_G
Cuts at: 0 26 199 558
Size: 26 173 359
Fragments arranged by size:
359 173 26
Enzymes that do cut:
AarI AccI ApoI AvaI BamHI BanI BbsI BfrBI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaWI
BseRI BseYI Bsp1286I BspMI BsrBI BstYI BtgI DraIII
EaeI Eco57MI EcoNI EcoO109I EcoRI HaeII MscI MslI
MspA1I NcoI NsiI NspI PflMI PfoI PstI SexAI
SfcI SmaI StyI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI
BciVI BclI BglII BlpI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI