Restriction map for
pF1KE1006
(Linear) (Six-base) MAPSORT of: KIEE1006.seq Check: 9528 from: 1 to: 561
KIEE1006.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AflIII A'CryG_T
Cuts at: 0 499 561
Size: 499 62
ApaI G_GGCC'C
Cuts at: 0 32 486 561
Size: 32 454 75
Fragments arranged by size:
454 75 32
ApoI r'AATT_y
Cuts at: 0 442 561
Size: 442 119
AvaI C'yCGr_G
Cuts at: 0 324 561
Size: 324 237
BamHI G'GATC_C
Cuts at: 0 231 561
Size: 231 330
BanII G_rGCy'C
Cuts at: 0 32 325 407 479 486 561
Size: 32 293 82 72 7 75
Fragments arranged by size:
293 82 75 72 32 7
BbvCI CC'TCA_GC
Cuts at: 0 13 412 561
Size: 13 399 149
Fragments arranged by size:
399 149 13
BglI GCCn_nnn'nGGC
Cuts at: 0 94 490 561
Size: 94 396 71
Fragments arranged by size:
396 94 71
Bme1580I G_kGCm'C
Cuts at: 0 32 343 486 533 561
Size: 32 311 143 47 28
Fragments arranged by size:
311 143 47 32 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 561
Size: 446 115
Bpu10I CC'TnA_GC
Cuts at: 0 13 412 478 561
Size: 13 399 66 83
Fragments arranged by size:
399 83 66 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 561
Size: 54 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 422 561
Size: 422 139
BsaAI yAC'GTr
Cuts at: 0 500 561
Size: 500 61
BsaHI Gr'CG_yC
Cuts at: 0 124 454 561
Size: 124 330 107
Fragments arranged by size:
330 124 107
BseYI C'CCAG_C
Cuts at: 0 108 220 561
Size: 108 112 341
Fragments arranged by size:
341 112 108
BsiHKAI G_wGCw'C
Cuts at: 0 479 561
Size: 479 82
Bsp1286I G_dGCh'C
Cuts at: 0 32 325 343 407 479 486 533 561
Size: 32 293 18 64 72 7 47 28
Fragments arranged by size:
293 72 64 47 32 28 18 7
BsrDI GCAATG_nn'
Cuts at: 0 288 561
Size: 288 273
BsrFI r'CCGG_y
Cuts at: 0 3 561
Size: 3 558
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 561
Size: 72 489
BstYI r'GATC_y
Cuts at: 0 231 561
Size: 231 330
BtgI C'CryG_G
Cuts at: 0 435 561
Size: 435 126
BtsI GCAGTG_nn'
Cuts at: 0 300 561
Size: 300 261
EcoICRI GAG'CTC
Cuts at: 0 477 561
Size: 477 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 561
Size: 446 115
EcoO109I rG'GnC_Cy
Cuts at: 0 29 328 482 483 561
Size: 29 299 154 1 78
Fragments arranged by size:
299 154 78 29 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 561
Size: 535 26
HincII GTy'rAC
Cuts at: 0 270 561
Size: 270 291
MslI CAynn'nnrTG
Cuts at: 0 356 561
Size: 356 205
MspA1I CmG'CkG
Cuts at: 0 72 549 561
Size: 72 477 12
Fragments arranged by size:
477 72 12
NspI r_CATG'y
Cuts at: 0 79 561
Size: 79 482
PmlI CAC'GTG
Cuts at: 0 500 561
Size: 500 61
PspOMI G'GGCC_C
Cuts at: 0 28 482 561
Size: 28 454 79
Fragments arranged by size:
454 79 28
PvuII CAG'CTG
Cuts at: 0 549 561
Size: 549 12
SacI G_AGCT'C
Cuts at: 0 479 561
Size: 479 82
SmlI C'TyrA_G
Cuts at: 0 33 561
Size: 33 528
StuI AGG'CCT
Cuts at: 0 347 561
Size: 347 214
StyI C'CwwG_G
Cuts at: 0 194 335 561
Size: 194 141 226
Fragments arranged by size:
226 194 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 561
Size: 145 416
Enzymes that do cut:
AflIII ApaI ApoI AvaI BamHI BanII BbvCI BglI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseYI
BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BstYI BtgI BtsI
EcoICRI Eco57MI EcoO109I Hin4I HincII MslI MspA1I NspI
PmlI PspOMI PvuII SacI SmlI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI