(Linear) (Six-base) MAPSORT of: KIEE1010.seq  Check: 5802  from: 1  to: 672



KIEE1010.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:28  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      28     672 
   Size:         28     644
 
ApaI G_GGCC'C

Cuts at:      0     122     672 
   Size:        122     550
 
ApoI r'AATT_y

Cuts at:      0     325     364     637     672 
   Size:        325      39     273      35

  Fragments arranged by size: 

                 325     273      39      35
 
AvaI C'yCGr_G

Cuts at:      0     218     672 
   Size:        218     454
 
BanI G'GyrC_C

Cuts at:      0     277     672 
   Size:        277     395
 
BanII G_rGCy'C

Cuts at:      0     122     672 
   Size:        122     550
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      65      99     672 
   Size:         65      34     573

  Fragments arranged by size: 

                 573      65      34
 
BfrBI ATG'CAT

Cuts at:      0     394     672 
   Size:        394     278
 
Bme1580I G_kGCm'C

Cuts at:      0     122     672 
   Size:        122     550
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     475     672 
   Size:        475     197
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     269     672 
   Size:        269     403
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     672 
   Size:         70     602
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324     672 
   Size:        324     348
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67      70      73     148     157     166     175     178 
   Size:         67       3       3      75       9       9       9       3

Cuts at:    178     181     193     196     199     208     211     247     672 
   Size:          3      12       3       3       9       3      36     425

  Fragments arranged by size: 

                 425      75      67      36      12       9       9       9
                   9       3       3       3       3       3       3       3
            
 
BsmI GAATG_Cn'

Cuts at:      0     396     672 
   Size:        396     276
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     672 
   Size:        122     550
 
BsrFI r'CCGG_y

Cuts at:      0     280     672 
   Size:        280     392
 
DraI TTT'AAA

Cuts at:      0     369     672 
   Size:        369     303
 
EaeI y'GGCC_r

Cuts at:      0     282     381     672 
   Size:        282      99     291

  Fragments arranged by size: 

                 291     282      99
 
EarI CTCTTCn'nnn_

Cuts at:      0     143     152     170     185     672 
   Size:        143       9      18      15     487

  Fragments arranged by size: 

                 487     143      18      15       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      40     672 
   Size:         40     632
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     672 
   Size:         70     602
 
EcoRI G'AATT_C

Cuts at:      0     637     672 
   Size:        637      35
 
HaeII r_GCGC'y

Cuts at:      0     208     672 
   Size:        208     464
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     672 
   Size:         25      32     615

  Fragments arranged by size: 

                 615      32      25
 
HincII GTy'rAC

Cuts at:      0     584     672 
   Size:        584      88
 
HindIII A'AGCT_T

Cuts at:      0     562     672 
   Size:        562     110
 
MslI CAynn'nnrTG

Cuts at:      0     292     672 
   Size:        292     380
 
NaeI GCC'GGC

Cuts at:      0     282     672 
   Size:        282     390
 
NgoMIV G'CCGG_C

Cuts at:      0     280     672 
   Size:        280     392
 
NsiI A_TGCA'T

Cuts at:      0     396     672 
   Size:        396     276
 
NspI r_CATG'y

Cuts at:      0     398     672 
   Size:        398     274
 
PspOMI G'GGCC_C

Cuts at:      0     118     672 
   Size:        118     554
 
SmlI C'TyrA_G

Cuts at:      0     303     672 
   Size:        303     369
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      45     672 
   Size:         45     627
 

 Enzymes that do cut:

     AlfI     ApaI     ApoI     AvaI     BanI    BanII     BcgI    BfrBI
 Bme1580I     BmrI     BplI     BpmI    BpuEI    BseRI     BsmI Bsp1286I
    BsrFI     DraI     EaeI     EarI     EciI  Eco57MI    EcoRI    HaeII
    Hin4I   HincII  HindIII     MslI     NaeI   NgoMIV     NsiI     NspI
   PspOMI     SmlI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI