Restriction map for
pF1KE1014
(Linear) (Six-base) MAPSORT of: KIEE1014.seq Check: 7455 from: 1 to: 906
KIEE1014.orf
With 182 enzymes: SgfI *
October 7, 2009 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 850 906
Size: 850 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 140 396 906
Size: 21 119 256 510
Fragments arranged by size:
510 256 119 21
AflIII A'CryG_T
Cuts at: 0 469 503 906
Size: 469 34 403
Fragments arranged by size:
469 403 34
AlwNI CAG_nnn'CTG
Cuts at: 0 549 675 906
Size: 549 126 231
Fragments arranged by size:
549 231 126
BanI G'GyrC_C
Cuts at: 0 862 906
Size: 862 44
BanII G_rGCy'C
Cuts at: 0 104 252 461 893 906
Size: 104 148 209 432 13
Fragments arranged by size:
432 209 148 104 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 906
Size: 558 348
BbvCI CC'TCA_GC
Cuts at: 0 607 906
Size: 607 299
BlpI GC'TnA_GC
Cuts at: 0 103 906
Size: 103 803
Bme1580I G_kGCm'C
Cuts at: 0 422 906
Size: 422 484
BmgBI CAC'GTC
Cuts at: 0 96 594 906
Size: 96 498 312
Fragments arranged by size:
498 312 96
Bpu10I CC'TnA_GC
Cuts at: 0 607 906
Size: 607 299
BsaAI yAC'GTr
Cuts at: 0 153 470 767 906
Size: 153 317 297 139
Fragments arranged by size:
317 297 153 139
BsaWI w'CCGG_w
Cuts at: 0 239 879 906
Size: 239 640 27
Fragments arranged by size:
640 239 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 906
Size: 430 30 446
Fragments arranged by size:
446 430 30
BseYI C'CCAG_C
Cuts at: 0 6 625 821 906
Size: 6 619 196 85
Fragments arranged by size:
619 196 85 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 906
Size: 849 57
BsiEI CG_ry'CG
Cuts at: 0 266 906
Size: 266 640
BsiHKAI G_wGCw'C
Cuts at: 0 104 461 893 906
Size: 104 357 432 13
Fragments arranged by size:
432 357 104 13
Bsp1286I G_dGCh'C
Cuts at: 0 104 252 422 461 893 906
Size: 104 148 170 39 432 13
Fragments arranged by size:
432 170 148 104 39 13
BspEI T'CCGG_A
Cuts at: 0 879 906
Size: 879 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 850 906
Size: 850 56
BsrFI r'CCGG_y
Cuts at: 0 261 265 745 906
Size: 261 4 480 161
Fragments arranged by size:
480 261 161 4
BsrGI T'GTAC_A
Cuts at: 0 472 906
Size: 472 434
BstEII G'GTnAC_C
Cuts at: 0 186 906
Size: 186 720
BtgI C'CryG_G
Cuts at: 0 182 906
Size: 182 724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 94 906
Size: 94 812
BtsI GCAGTG_nn'
Cuts at: 0 333 391 906
Size: 333 58 515
Fragments arranged by size:
515 333 58
DraI TTT'AAA
Cuts at: 0 140 906
Size: 140 766
EaeI y'GGCC_r
Cuts at: 0 263 761 812 897 906
Size: 263 498 51 85 9
Fragments arranged by size:
498 263 85 51 9
EagI C'GGCC_G
Cuts at: 0 263 906
Size: 263 643
EarI CTCTTCn'nnn_
Cuts at: 0 31 40 906
Size: 31 9 866
Fragments arranged by size:
866 31 9
EcoICRI GAG'CTC
Cuts at: 0 102 459 891 906
Size: 102 357 432 15
Fragments arranged by size:
432 357 102 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 140 396 906
Size: 21 119 256 510
Fragments arranged by size:
510 256 119 21
HaeII r_GCGC'y
Cuts at: 0 434 906
Size: 434 472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 707 739 906
Size: 707 32 167
Fragments arranged by size:
707 167 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 906
Size: 870 36
MscI TGG'CCA
Cuts at: 0 814 899 906
Size: 814 85 7
Fragments arranged by size:
814 85 7
MslI CAynn'nnrTG
Cuts at: 0 854 906
Size: 854 52
MspA1I CmG'CkG
Cuts at: 0 107 906
Size: 107 799
NaeI GCC'GGC
Cuts at: 0 267 906
Size: 267 639
NgoMIV G'CCGG_C
Cuts at: 0 265 906
Size: 265 641
NspI r_CATG'y
Cuts at: 0 273 400 579 906
Size: 273 127 179 327
Fragments arranged by size:
327 273 179 127
PasI CC'CwG_GG
Cuts at: 0 357 906
Size: 357 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 821 906
Size: 357 464 85
Fragments arranged by size:
464 357 85
PfoI T'CCnGG_A
Cuts at: 0 15 365 906
Size: 15 350 541
Fragments arranged by size:
541 350 15
PmlI CAC'GTG
Cuts at: 0 470 767 906
Size: 470 297 139
Fragments arranged by size:
470 297 139
PstI C_TGCA'G
Cuts at: 0 554 668 906
Size: 554 114 238
Fragments arranged by size:
554 238 114
PvuII CAG'CTG
Cuts at: 0 107 906
Size: 107 799
SacI G_AGCT'C
Cuts at: 0 104 461 893 906
Size: 104 357 432 13
Fragments arranged by size:
432 357 104 13
SapI GCTCTTCn'nnn_
Cuts at: 0 31 40 906
Size: 31 9 866
Fragments arranged by size:
866 31 9
SfcI C'TryA_G
Cuts at: 0 550 664 906
Size: 550 114 242
Fragments arranged by size:
550 242 114
SnaBI TAC'GTA
Cuts at: 0 153 906
Size: 153 753
SphI G_CATG'C
Cuts at: 0 273 906
Size: 273 633
StyI C'CwwG_G
Cuts at: 0 215 906
Size: 215 691
SwaI ATTT'AAAT
Cuts at: 0 140 906
Size: 140 766
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 851 906
Size: 813 38 55
Fragments arranged by size:
813 55 38
TatI w'GTAC_w
Cuts at: 0 472 506 906
Size: 472 34 400
Fragments arranged by size:
472 400 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 717 749 906
Size: 430 32 255 32 157
Fragments arranged by size:
430 255 157 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 562 595 906
Size: 562 33 311
Fragments arranged by size:
562 311 33
Enzymes that do cut:
AarI AcuI AflIII AlwNI BanI BanII BbsI BbvCI
BlpI Bme1580I BmgBI Bpu10I BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI BsrGI
BstEII BtgI BtgZI BtsI DraI EaeI EagI EarI
EcoICRI Eco57MI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PmlI PstI
PvuII SacI SapI SfcI SnaBI SphI StyI SwaI
TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI BpmI
BpuEI BsaI BsaBI BsaHI BseRI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SrfI
SspI StuI XbaI XcmI XhoI XmaI XmnI ZraI