Restriction map for
pF1KE1019
(Linear) (Six-base) MAPSORT of: KIEE1019.seq Check: 9968 from: 1 to: 1989
KIEE1019.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AatII G_ACGT'C
Cuts at: 0 512 1989
Size: 512 1477
Acc65I G'GTAC_C
Cuts at: 0 814 1989
Size: 814 1175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1381 1675 1808 1989
Size: 1228 153 294 133 181
Fragments arranged by size:
1228 294 181 153 133
AfeI AGC'GCT
Cuts at: 0 983 1989
Size: 983 1006
AlwNI CAG_nnn'CTG
Cuts at: 0 1239 1989
Size: 1239 750
ApoI r'AATT_y
Cuts at: 0 1375 1501 1989
Size: 1375 126 488
Fragments arranged by size:
1375 488 126
AvaI C'yCGr_G
Cuts at: 0 108 1989
Size: 108 1881
BamHI G'GATC_C
Cuts at: 0 1094 1989
Size: 1094 895
BanI G'GyrC_C
Cuts at: 0 814 1989
Size: 814 1175
BanII G_rGCy'C
Cuts at: 0 739 1989
Size: 739 1250
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 1989
Size: 233 1756
BfrBI ATG'CAT
Cuts at: 0 1471 1989
Size: 1471 518
BglI GCCn_nnn'nGGC
Cuts at: 0 1270 1989
Size: 1270 719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 1989
Size: 480 1509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 539 715 1121 1989
Size: 401 138 176 406 868
Fragments arranged by size:
868 406 401 176 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1840 1989
Size: 495 1345 149
Fragments arranged by size:
1345 495 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 468 1989
Size: 468 1521
BsaBI GATnn'nnATC
Cuts at: 0 1940 1989
Size: 1940 49
BsaHI Gr'CG_yC
Cuts at: 0 509 667 1989
Size: 509 158 1322
Fragments arranged by size:
1322 509 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 1989
Size: 676 1313
BseYI C'CCAG_C
Cuts at: 0 49 1232 1516 1989
Size: 49 1183 284 473
Fragments arranged by size:
1183 473 284 49
BsiHKAI G_wGCw'C
Cuts at: 0 739 1989
Size: 739 1250
BsmI GAATG_Cn'
Cuts at: 0 1022 1989
Size: 1022 967
BsmBI CGTCTCn'nnnn_
Cuts at: 0 451 658 1989
Size: 451 207 1331
Fragments arranged by size:
1331 451 207
Bsp1286I G_dGCh'C
Cuts at: 0 739 1989
Size: 739 1250
BspHI T'CATG_A
Cuts at: 0 1424 1989
Size: 1424 565
BsrDI GCAATG_nn'
Cuts at: 0 1442 1989
Size: 1442 547
BsrFI r'CCGG_y
Cuts at: 0 349 1989
Size: 349 1640
BstBI TT'CG_AA
Cuts at: 0 1759 1989
Size: 1759 230
BstEII G'GTnAC_C
Cuts at: 0 829 1989
Size: 829 1160
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1837 1989
Size: 1837 152
BstYI r'GATC_y
Cuts at: 0 384 1094 1989
Size: 384 710 895
Fragments arranged by size:
895 710 384
Bsu36I CC'TnA_GG
Cuts at: 0 659 1724 1989
Size: 659 1065 265
Fragments arranged by size:
1065 659 265
BtgI C'CryG_G
Cuts at: 0 1769 1989
Size: 1769 220
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 782 1989
Size: 782 1207
BtsI GCAGTG_nn'
Cuts at: 0 1237 1989
Size: 1237 752
ClaI AT'CG_AT
Cuts at: 0 186 1989
Size: 186 1803
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 807 842 1989
Size: 807 35 1147
Fragments arranged by size:
1147 807 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 67 1989
Size: 67 1922
EaeI y'GGCC_r
Cuts at: 0 123 1989
Size: 123 1866
EarI CTCTTCn'nnn_
Cuts at: 0 326 370 603 635 751 1055 1219 1989
Size: 326 44 233 32 116 304 164 770
Fragments arranged by size:
770 326 304 233 164 116 44 32
EcoICRI GAG'CTC
Cuts at: 0 737 1989
Size: 737 1252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 539 715 1121 1228 1381 1675 1808
Size: 401 138 176 406 107 153 294 133
Cuts at: 1808 1989
Size: 181
Fragments arranged by size:
406 401 294 181 176 153 138 133
107
EcoO109I rG'GnC_Cy
Cuts at: 0 1365 1989
Size: 1365 624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 355 1989
Size: 355 1634
HaeII r_GCGC'y
Cuts at: 0 985 1989
Size: 985 1004
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 607 639 1989
Size: 202 32 373 32 1350
Fragments arranged by size:
1350 373 202 32 32
KpnI G_GTAC'C
Cuts at: 0 818 1989
Size: 818 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1989
Size: 1312 677
MscI TGG'CCA
Cuts at: 0 125 1989
Size: 125 1864
MslI CAynn'nnrTG
Cuts at: 0 1927 1989
Size: 1927 62
MspA1I CmG'CkG
Cuts at: 0 53 1516 1989
Size: 53 1463 473
Fragments arranged by size:
1463 473 53
NcoI C'CATG_G
Cuts at: 0 1769 1989
Size: 1769 220
NsiI A_TGCA'T
Cuts at: 0 1473 1989
Size: 1473 516
PasI CC'CwG_GG
Cuts at: 0 21 1989
Size: 21 1968
PflMI CCAn_nnn'nTGG
Cuts at: 0 27 1989
Size: 27 1962
PsiI TTA'TAA
Cuts at: 0 1972 1989
Size: 1972 17
PspXI vC'TCGA_Gb
Cuts at: 0 108 1989
Size: 108 1881
PvuII CAG'CTG
Cuts at: 0 1516 1989
Size: 1516 473
SacI G_AGCT'C
Cuts at: 0 739 1989
Size: 739 1250
SapI GCTCTTCn'nnn_
Cuts at: 0 751 1219 1989
Size: 751 468 770
Fragments arranged by size:
770 751 468
SfcI C'TryA_G
Cuts at: 0 1306 1989
Size: 1306 683
SmlI C'TyrA_G
Cuts at: 0 108 474 1855 1989
Size: 108 366 1381 134
Fragments arranged by size:
1381 366 134 108
SspI AAT'ATT
Cuts at: 0 1896 1989
Size: 1896 93
StuI AGG'CCT
Cuts at: 0 1085 1989
Size: 1085 904
StyI C'CwwG_G
Cuts at: 0 603 740 1769 1989
Size: 603 137 1029 220
Fragments arranged by size:
1029 603 220 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1688 1989
Size: 1688 301
TatI w'GTAC_w
Cuts at: 0 128 1702 1809 1989
Size: 128 1574 107 180
Fragments arranged by size:
1574 180 128 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 1989
Size: 319 32 1638
Fragments arranged by size:
1638 319 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 1961 1989
Size: 162 1799 28
Fragments arranged by size:
1799 162 28
XhoI C'TCGA_G
Cuts at: 0 108 1989
Size: 108 1881
XmnI GAAnn'nnTTC
Cuts at: 0 1213 1989
Size: 1213 776
ZraI GAC'GTC
Cuts at: 0 510 1989
Size: 510 1479
Enzymes that do cut:
AatII Acc65I AcuI AfeI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BfrBI BglI BplI BpmI BpuEI
BsaI BsaBI BsaHI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrDI BsrFI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NcoI NsiI PasI PflMI
PsiI PspXI PvuII SacI SapI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstZ17I DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XmaI