Restriction map for
pF1KE1020
(Linear) (Six-base) MAPSORT of: KIEE1020.seq Check: 2116 from: 1 to: 2004
KIEE1020.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1719 1821 2004
Size: 1719 102 183
Fragments arranged by size:
1719 183 102
AccI GT'mk_AC
Cuts at: 0 763 959 2004
Size: 763 196 1045
Fragments arranged by size:
1045 763 196
Acc65I G'GTAC_C
Cuts at: 0 1951 2004
Size: 1951 53
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 507 1241 1371 1500 1610 2004
Size: 135 372 734 130 129 110 394
Fragments arranged by size:
734 394 372 135 130 129 110
AflIII A'CryG_T
Cuts at: 0 962 2004
Size: 962 1042
AgeI A'CCGG_T
Cuts at: 0 1268 2004
Size: 1268 736
AhdI GACnn_n'nnGTC
Cuts at: 0 978 2004
Size: 978 1026
AleI CACnn'nnGTG
Cuts at: 0 277 2004
Size: 277 1727
AlwNI CAG_nnn'CTG
Cuts at: 0 99 462 530 2004
Size: 99 363 68 1474
Fragments arranged by size:
1474 363 99 68
ApaI G_GGCC'C
Cuts at: 0 507 1153 1705 2004
Size: 507 646 552 299
Fragments arranged by size:
646 552 507 299
ApoI r'AATT_y
Cuts at: 0 718 2004
Size: 718 1286
AvaI C'yCGr_G
Cuts at: 0 368 497 1309 1331 1367 1698 2004
Size: 368 129 812 22 36 331 306
Fragments arranged by size:
812 368 331 306 129 36 22
BanI G'GyrC_C
Cuts at: 0 1178 1240 1421 1661 1833 1951 2004
Size: 1178 62 181 240 172 118 53
Fragments arranged by size:
1178 240 181 172 118 62 53
BanII G_rGCy'C
Cuts at: 0 507 539 1021 1063 1153 1374 1583 1705
Size: 507 32 482 42 90 221 209 122
Cuts at: 1705 1818 1903 2003 2004
Size: 113 85 100 1
Fragments arranged by size:
507 482 221 209 122 113 100 90
85 42 32 1
BbeI G_GCGC'C
Cuts at: 0 1182 1665 2004
Size: 1182 483 339
Fragments arranged by size:
1182 483 339
BbvCI CC'TCA_GC
Cuts at: 0 911 1126 1315 1969 2004
Size: 911 215 189 654 35
Fragments arranged by size:
911 654 215 189 35
BglI GCCn_nnn'nGGC
Cuts at: 0 502 1115 1148 1850 1859 2004
Size: 502 613 33 702 9 145
Fragments arranged by size:
702 613 502 145 33 9
BglII A'GATC_T
Cuts at: 0 1893 2004
Size: 1893 111
BlpI GC'TnA_GC
Cuts at: 0 322 2004
Size: 322 1682
Bme1580I G_kGCm'C
Cuts at: 0 507 737 830 1153 1245 1424 1644 1673
Size: 507 230 93 323 92 179 220 29
Cuts at: 1673 1705 1838 2004
Size: 32 133 166
Fragments arranged by size:
507 323 230 220 179 166 133 93
92 32 29
BmgBI CAC'GTC
Cuts at: 0 671 963 2004
Size: 671 292 1041
Fragments arranged by size:
1041 671 292
BmrI ACTGGGnnnn_n'
Cuts at: 0 661 817 1246 1796 2004
Size: 661 156 429 550 208
Fragments arranged by size:
661 550 429 208 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 1888 2004
Size: 523 1365 116
Fragments arranged by size:
1365 523 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 733 1069 1482 1759 1832 1963 2004
Size: 6 727 336 413 277 73 131 41
Fragments arranged by size:
727 413 336 277 131 73 41 6
Bpu10I CC'TnA_GC
Cuts at: 0 629 911 1126 1315 1398 1969 2004
Size: 629 282 215 189 83 571 35
Fragments arranged by size:
629 571 282 215 189 83 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1561 2004
Size: 493 1068 443
Fragments arranged by size:
1068 493 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 986 2004
Size: 986 1018
BsaAI yAC'GTr
Cuts at: 0 800 1639 2004
Size: 800 839 365
Fragments arranged by size:
839 800 365
BsaHI Gr'CG_yC
Cuts at: 0 1179 1662 1920 2004
Size: 1179 483 258 84
Fragments arranged by size:
1179 483 258 84
BsaWI w'CCGG_w
Cuts at: 0 1268 2004
Size: 1268 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 1024 1054 2004
Size: 793 30 201 30 950
Fragments arranged by size:
950 793 201 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 896 1421 2004
Size: 421 475 525 583
Fragments arranged by size:
583 525 475 421
BseYI C'CCAG_C
Cuts at: 0 46 108 214 681 1347 2004
Size: 46 62 106 467 666 657
Fragments arranged by size:
666 657 467 106 62 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1691 2004
Size: 651 1040 313
Fragments arranged by size:
1040 651 313
BsiHKAI G_wGCw'C
Cuts at: 0 128 311 388 1818 1903 2004
Size: 128 183 77 1430 85 101
Fragments arranged by size:
1430 183 128 101 85 77
BsmI GAATG_Cn'
Cuts at: 0 360 2004
Size: 360 1644
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1927 2004
Size: 1927 77
Bsp1286I G_dGCh'C
Cuts at: 0 128 311 388 507 539 737 830 1021
Size: 128 183 77 119 32 198 93 191
Cuts at: 1021 1063 1153 1245 1374 1424 1583 1644 1673
Size: 42 90 92 129 50 159 61 29
Cuts at: 1673 1705 1818 1838 1903 2003 2004
Size: 32 113 20 65 100 1
Fragments arranged by size:
198 191 183 159 129 128 119 113
100 93 92 90 77 65 61 50
42 32 32 29 20 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 775 1719 1821 2004
Size: 524 251 944 102 183
Fragments arranged by size:
944 524 251 183 102
BsrBI CCG'CTC
Cuts at: 0 29 633 1199 2004
Size: 29 604 566 805
Fragments arranged by size:
805 604 566 29
BsrFI r'CCGG_y
Cuts at: 0 41 1114 1268 1424 1853 2004
Size: 41 1073 154 156 429 151
Fragments arranged by size:
1073 429 156 154 151 41
BsrGI T'GTAC_A
Cuts at: 0 562 2004
Size: 562 1442
BssSI C'ACGA_G
Cuts at: 0 610 927 1038 2004
Size: 610 317 111 966
Fragments arranged by size:
966 610 317 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 2004
Size: 99 1905
BstEII G'GTnAC_C
Cuts at: 0 144 1003 2004
Size: 144 859 1001
Fragments arranged by size:
1001 859 144
BstYI r'GATC_y
Cuts at: 0 1893 2004
Size: 1893 111
BstZ17I GTA'TAC
Cuts at: 0 764 2004
Size: 764 1240
BtgI C'CryG_G
Cuts at: 0 812 968 1073 1174 1186 1465 2004
Size: 812 156 105 101 12 279 539
Fragments arranged by size:
812 539 279 156 105 101 12
BtsI GCAGTG_nn'
Cuts at: 0 1505 2004
Size: 1505 499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 885 920 2004
Size: 885 35 1084
Fragments arranged by size:
1084 885 35
DraIII CAC_nnn'GTG
Cuts at: 0 1240 2004
Size: 1240 764
EaeI y'GGCC_r
Cuts at: 0 265 1282 1744 1860 1932 2004
Size: 265 1017 462 116 72 72
Fragments arranged by size:
1017 462 265 116 72 72
EarI CTCTTCn'nnn_
Cuts at: 0 171 526 1606 1914 2004
Size: 171 355 1080 308 90
Fragments arranged by size:
1080 355 308 171 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 61 1594 2004
Size: 61 1533 410
Fragments arranged by size:
1533 410 61
EcoICRI GAG'CTC
Cuts at: 0 1816 1901 2004
Size: 1816 85 103
Fragments arranged by size:
1816 103 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 135 507 733 1069 1241 1371 1482
Size: 6 129 372 226 336 172 130 111
Cuts at: 1482 1500 1610 1759 1832 1963 2004
Size: 18 110 149 73 131 41
Fragments arranged by size:
372 336 226 172 149 131 130 129
111 110 73 41 18 6
EcoO109I rG'GnC_Cy
Cuts at: 0 57 96 197 503 504 655 1149 1150
Size: 57 39 101 306 1 151 494 1
Cuts at: 1150 1297 1326 1521 1702 1728 1755 2004
Size: 147 29 195 181 26 27 249
Fragments arranged by size:
494 306 249 195 181 151 147 101
57 39 29 27 26 1 1
EcoRV GAT'ATC
Cuts at: 0 1781 2004
Size: 1781 223
FseI GG_CCGG'CC
Cuts at: 0 1857 2004
Size: 1857 147
HaeII r_GCGC'y
Cuts at: 0 1182 1665 2004
Size: 1182 483 339
Fragments arranged by size:
1182 483 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 964 996 1800 1832 1885 1917 2004
Size: 964 32 804 32 53 32 87
Fragments arranged by size:
964 804 87 53 32 32 32
KasI G'GCGC_C
Cuts at: 0 1178 1661 2004
Size: 1178 483 343
Fragments arranged by size:
1178 483 343
KpnI G_GTAC'C
Cuts at: 0 1955 2004
Size: 1955 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1010 1253 2004
Size: 402 608 243 751
Fragments arranged by size:
751 608 402 243
MscI TGG'CCA
Cuts at: 0 1746 2004
Size: 1746 258
MslI CAynn'nnrTG
Cuts at: 0 139 277 2004
Size: 139 138 1727
Fragments arranged by size:
1727 139 138
MspA1I CmG'CkG
Cuts at: 0 977 1252 1262 1455 1616 1742 1886 2004
Size: 977 275 10 193 161 126 144 118
Fragments arranged by size:
977 275 193 161 144 126 118 10
NaeI GCC'GGC
Cuts at: 0 43 1116 1855 2004
Size: 43 1073 739 149
Fragments arranged by size:
1073 739 149 43
NarI GG'CG_CC
Cuts at: 0 1179 1662 2004
Size: 1179 483 342
Fragments arranged by size:
1179 483 342
NcoI C'CATG_G
Cuts at: 0 812 968 2004
Size: 812 156 1036
Fragments arranged by size:
1036 812 156
NgoMIV G'CCGG_C
Cuts at: 0 41 1114 1853 2004
Size: 41 1073 739 151
Fragments arranged by size:
1073 739 151 41
PasI CC'CwG_GG
Cuts at: 0 1012 1289 1722 2004
Size: 1012 277 433 282
Fragments arranged by size:
1012 433 282 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 1272 1722 2004
Size: 1272 450 282
Fragments arranged by size:
1272 450 282
PfoI T'CCnGG_A
Cuts at: 0 256 1783 2004
Size: 256 1527 221
Fragments arranged by size:
1527 256 221
PpuMI rG'GwC_Cy
Cuts at: 0 197 655 1297 1326 1521 1728 2004
Size: 197 458 642 29 195 207 276
Fragments arranged by size:
642 458 276 207 197 195 29
PspOMI G'GGCC_C
Cuts at: 0 503 1149 1701 2004
Size: 503 646 552 303
Fragments arranged by size:
646 552 503 303
PstI C_TGCA'G
Cuts at: 0 95 772 1058 1572 2004
Size: 95 677 286 514 432
Fragments arranged by size:
677 514 432 286 95
PvuII CAG'CTG
Cuts at: 0 1252 1262 1742 1886 2004
Size: 1252 10 480 144 118
Fragments arranged by size:
1252 480 144 118 10
SacI G_AGCT'C
Cuts at: 0 1818 1903 2004
Size: 1818 85 101
Fragments arranged by size:
1818 101 85
SanDI GG'GwC_CC
Cuts at: 0 1297 1326 2004
Size: 1297 29 678
Fragments arranged by size:
1297 678 29
SapI GCTCTTCn'nnn_
Cuts at: 0 526 1914 2004
Size: 526 1388 90
Fragments arranged by size:
1388 526 90
SfcI C'TryA_G
Cuts at: 0 91 768 1054 1568 2004
Size: 91 677 286 514 436
Fragments arranged by size:
677 514 436 286 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1148 1859 2004
Size: 1148 711 145
Fragments arranged by size:
1148 711 145
SfoI GGC'GCC
Cuts at: 0 1180 1663 2004
Size: 1180 483 341
Fragments arranged by size:
1180 483 341
SmaI CCC'GGG
Cuts at: 0 1333 2004
Size: 1333 671
SmlI C'TyrA_G
Cuts at: 0 508 1540 2004
Size: 508 1032 464
Fragments arranged by size:
1032 508 464
StyI C'CwwG_G
Cuts at: 0 812 968 2004
Size: 812 156 1036
Fragments arranged by size:
1036 812 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1228 1281 2004
Size: 1228 53 723
Fragments arranged by size:
1228 723 53
TatI w'GTAC_w
Cuts at: 0 562 2004
Size: 562 1442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1024 1056 2004
Size: 1024 32 948
Fragments arranged by size:
1024 948 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 708 2004
Size: 708 1296
XmaI C'CCGG_G
Cuts at: 0 1331 2004
Size: 1331 673
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstEII BstYI BstZ17I BtgI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FseI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SfcI SfiI SfoI SmaI SmlI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BmtI BsaBI BsiEI BsiWI BspEI BspHI
BsrDI BssHII BstBI BstXI Bsu36I BtgZI ClaI DraI
DrdI EagI EcoNI EcoRI FalI FspI FspAI HincII
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI