Restriction map for
pF1KE1023
(Linear) (Six-base) MAPSORT of: KIEE1023.seq Check: 2440 from: 1 to: 2322
KIEE1023.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2235 2322
Size: 2235 87
AatII G_ACGT'C
Cuts at: 0 1177 2322
Size: 1177 1145
AccI GT'mk_AC
Cuts at: 0 515 2048 2322
Size: 515 1533 274
Fragments arranged by size:
1533 515 274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 940 2322
Size: 736 204 1382
Fragments arranged by size:
1382 736 204
AflIII A'CryG_T
Cuts at: 0 2021 2322
Size: 2021 301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 965 1579 2322
Size: 965 614 743
Fragments arranged by size:
965 743 614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 2322
Size: 311 32 1979
Fragments arranged by size:
1979 311 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1753 2124 2322
Size: 1753 371 198
Fragments arranged by size:
1753 371 198
ApaI G_GGCC'C
Cuts at: 0 289 473 971 1202 2322
Size: 289 184 498 231 1120
Fragments arranged by size:
1120 498 289 231 184
ApoI r'AATT_y
Cuts at: 0 554 2322
Size: 554 1768
AvaI C'yCGr_G
Cuts at: 0 2095 2174 2322
Size: 2095 79 148
Fragments arranged by size:
2095 148 79
BamHI G'GATC_C
Cuts at: 0 2226 2322
Size: 2226 96
BanI G'GyrC_C
Cuts at: 0 2078 2247 2322
Size: 2078 169 75
Fragments arranged by size:
2078 169 75
BanII G_rGCy'C
Cuts at: 0 77 289 427 473 548 971 1202 1276
Size: 77 212 138 46 75 423 231 74
Cuts at: 1276 1987 2322
Size: 711 335
Fragments arranged by size:
711 423 335 231 212 138 77 75
74 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 2322
Size: 203 2119
BbvCI CC'TCA_GC
Cuts at: 0 1746 2009 2322
Size: 1746 263 313
Fragments arranged by size:
1746 313 263
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1937 1971 2322
Size: 1937 34 351
Fragments arranged by size:
1937 351 34
BclI T'GATC_A
Cuts at: 0 1559 2322
Size: 1559 763
BglI GCCn_nnn'nGGC
Cuts at: 0 900 2322
Size: 900 1422
BglII A'GATC_T
Cuts at: 0 582 2322
Size: 582 1740
Bme1580I G_kGCm'C
Cuts at: 0 289 473 971 1202 2252 2322
Size: 289 184 498 231 1050 70
Fragments arranged by size:
1050 498 289 231 184 70
BmgBI CAC'GTC
Cuts at: 0 2022 2322
Size: 2022 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 607 971 1018 2322
Size: 607 364 47 1304
Fragments arranged by size:
1304 607 364 47
BmtI G_CTAG'C
Cuts at: 0 1955 2322
Size: 1955 367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1404 2322
Size: 1404 918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 412 983 1569 2036 2259 2322
Size: 70 342 571 586 467 223 63
Fragments arranged by size:
586 571 467 342 223 70 63
Bpu10I CC'TnA_GC
Cuts at: 0 649 1552 1746 2009 2322
Size: 649 903 194 263 313
Fragments arranged by size:
903 649 313 263 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1301 2322
Size: 607 694 1021
Fragments arranged by size:
1021 694 607
BsaHI Gr'CG_yC
Cuts at: 0 1174 2276 2322
Size: 1174 1102 46
Fragments arranged by size:
1174 1102 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 198 556 756 986 1734 1749 2175 2322
Size: 198 358 200 230 748 15 426 147
Fragments arranged by size:
748 426 358 230 200 198 147 15
BseYI C'CCAG_C
Cuts at: 0 1231 1862 1967 2222 2322
Size: 1231 631 105 255 100
Fragments arranged by size:
1231 631 255 105 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 2322
Size: 335 1987
BsiHKAI G_wGCw'C
Cuts at: 0 297 548 1276 1987 2322
Size: 297 251 728 711 335
Fragments arranged by size:
728 711 335 297 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2012 2322
Size: 2012 310
Bsp1286I G_dGCh'C
Cuts at: 0 77 289 297 427 473 548 971 1202
Size: 77 212 8 130 46 75 423 231
Cuts at: 1202 1276 1987 2252 2322
Size: 74 711 265 70
Fragments arranged by size:
711 423 265 231 212 130 77 75
74 70 46 8
BspHI T'CATG_A
Cuts at: 0 2163 2322
Size: 2163 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 1724 2235 2322
Size: 315 1409 511 87
Fragments arranged by size:
1409 511 315 87
BsrBI CCG'CTC
Cuts at: 0 2254 2322
Size: 2254 68
BsrDI GCAATG_nn'
Cuts at: 0 287 1191 2319 2322
Size: 287 904 1128 3
Fragments arranged by size:
1128 904 287 3
BsrFI r'CCGG_y
Cuts at: 0 507 2322
Size: 507 1815
BssSI C'ACGA_G
Cuts at: 0 108 2322
Size: 108 2214
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1851 2322
Size: 1851 471
BstXI CCAn_nnnn'nTGG
Cuts at: 0 739 2322
Size: 739 1583
BstYI r'GATC_y
Cuts at: 0 582 755 2004 2226 2322
Size: 582 173 1249 222 96
Fragments arranged by size:
1249 582 222 173 96
BtgI C'CryG_G
Cuts at: 0 53 1598 1763 2322
Size: 53 1545 165 559
Fragments arranged by size:
1545 559 165 53
BtsI GCAGTG_nn'
Cuts at: 0 1054 1751 1769 2322
Size: 1054 697 18 553
Fragments arranged by size:
1054 697 553 18
EaeI y'GGCC_r
Cuts at: 0 375 1766 2322
Size: 375 1391 556
Fragments arranged by size:
1391 556 375
EarI CTCTTCn'nnn_
Cuts at: 0 608 2322
Size: 608 1714
EcoICRI GAG'CTC
Cuts at: 0 546 1274 1985 2322
Size: 546 728 711 337
Fragments arranged by size:
728 711 546 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 412 736 940 983 1569 2036 2259
Size: 70 342 324 204 43 586 467 223
Cuts at: 2259 2322
Size: 63
Fragments arranged by size:
586 467 342 324 223 204 70 63
43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 998 2322
Size: 998 1324
EcoO109I rG'GnC_Cy
Cuts at: 0 327 469 967 1198 1657 2121 2322
Size: 327 142 498 231 459 464 201
Fragments arranged by size:
498 464 459 327 231 201 142
HaeII r_GCGC'y
Cuts at: 0 2212 2322
Size: 2212 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 330 362 1320 1352 2287 2319 2322
Size: 330 32 958 32 935 32 3
Fragments arranged by size:
958 935 330 32 32 32 3
HindIII A'AGCT_T
Cuts at: 0 2062 2322
Size: 2062 260
MscI TGG'CCA
Cuts at: 0 377 2322
Size: 377 1945
MslI CAynn'nnrTG
Cuts at: 0 1561 2322
Size: 1561 761
MspA1I CmG'CkG
Cuts at: 0 918 1050 1785 2222 2322
Size: 918 132 735 437 100
Fragments arranged by size:
918 735 437 132 100
NaeI GCC'GGC
Cuts at: 0 509 2322
Size: 509 1813
NcoI C'CATG_G
Cuts at: 0 53 2322
Size: 53 2269
NgoMIV G'CCGG_C
Cuts at: 0 507 2322
Size: 507 1815
NheI G'CTAG_C
Cuts at: 0 1951 2322
Size: 1951 371
NspI r_CATG'y
Cuts at: 0 39 747 1110 2322
Size: 39 708 363 1212
Fragments arranged by size:
1212 708 363 39
PasI CC'CwG_GG
Cuts at: 0 1194 1354 2124 2213 2322
Size: 1194 160 770 89 109
Fragments arranged by size:
1194 770 160 109 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 2124 2322
Size: 2124 198
PfoI T'CCnGG_A
Cuts at: 0 86 999 2322
Size: 86 913 1323
Fragments arranged by size:
1323 913 86
PpuMI rG'GwC_Cy
Cuts at: 0 327 1657 2322
Size: 327 1330 665
Fragments arranged by size:
1330 665 327
PspOMI G'GGCC_C
Cuts at: 0 285 469 967 1198 2322
Size: 285 184 498 231 1124
Fragments arranged by size:
1124 498 285 231 184
PstI C_TGCA'G
Cuts at: 0 326 1721 1790 1844 1950 2322
Size: 326 1395 69 54 106 372
Fragments arranged by size:
1395 372 326 106 69 54
PvuII CAG'CTG
Cuts at: 0 918 1785 2222 2322
Size: 918 867 437 100
Fragments arranged by size:
918 867 437 100
SacI G_AGCT'C
Cuts at: 0 548 1276 1987 2322
Size: 548 728 711 335
Fragments arranged by size:
728 711 548 335
SanDI GG'GwC_CC
Cuts at: 0 1657 2322
Size: 1657 665
SapI GCTCTTCn'nnn_
Cuts at: 0 608 2322
Size: 608 1714
ScaI AGT'ACT
Cuts at: 0 306 2322
Size: 306 2016
SfcI C'TryA_G
Cuts at: 0 322 449 512 1717 1786 1840 1946 2322
Size: 322 127 63 1205 69 54 106 376
Fragments arranged by size:
1205 376 322 127 106 69 63 54
SmlI C'TyrA_G
Cuts at: 0 586 1316 2322
Size: 586 730 1006
Fragments arranged by size:
1006 730 586
SphI G_CATG'C
Cuts at: 0 39 2322
Size: 39 2283
StuI AGG'CCT
Cuts at: 0 255 1741 2236 2322
Size: 255 1486 495 86
Fragments arranged by size:
1486 495 255 86
StyI C'CwwG_G
Cuts at: 0 53 2322
Size: 53 2269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1055 2322
Size: 1055 1267
TatI w'GTAC_w
Cuts at: 0 304 2322
Size: 304 2018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 2322
Size: 740 32 1550
Fragments arranged by size:
1550 740 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 896 2322
Size: 896 1426
ZraI GAC'GTC
Cuts at: 0 1175 2322
Size: 1175 1147
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BclI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MscI
MslI MspA1I NaeI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SapI ScaI SfcI SmlI SphI StuI StyI TaqII
TatI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BfrBI
BlpI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BspEI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI