Restriction map for
pF1KE1024
(Linear) (Six-base) MAPSORT of: KIEE1024.seq Check: 69 from: 1 to: 2424
KIEE1024.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1532 2424
Size: 1532 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 2424
Size: 1315 1109
AflIII A'CryG_T
Cuts at: 0 1010 1625 2424
Size: 1010 615 799
Fragments arranged by size:
1010 799 615
AleI CACnn'nnGTG
Cuts at: 0 154 265 2424
Size: 154 111 2159
Fragments arranged by size:
2159 154 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 608 1394 2424
Size: 608 786 1030
Fragments arranged by size:
1030 786 608
AlwNI CAG_nnn'CTG
Cuts at: 0 352 646 1022 1116 1281 2080 2424
Size: 352 294 376 94 165 799 344
Fragments arranged by size:
799 376 352 344 294 165 94
ApaI G_GGCC'C
Cuts at: 0 95 1963 2028 2363 2424
Size: 95 1868 65 335 61
Fragments arranged by size:
1868 335 95 65 61
AvaI C'yCGr_G
Cuts at: 0 74 83 163 189 776 945 1256 1916
Size: 74 9 80 26 587 169 311 660
Cuts at: 1916 2009 2424
Size: 93 415
Fragments arranged by size:
660 587 415 311 169 93 80 74
26 9
BamHI G'GATC_C
Cuts at: 0 1079 2424
Size: 1079 1345
BanI G'GyrC_C
Cuts at: 0 15 46 487 1397 2424
Size: 15 31 441 910 1027
Fragments arranged by size:
1027 910 441 31 15
BanII G_rGCy'C
Cuts at: 0 95 341 375 742 865 874 964 998
Size: 95 246 34 367 123 9 90 34
Cuts at: 998 1179 1696 1963 2028 2363 2424
Size: 181 517 267 65 335 61
Fragments arranged by size:
517 367 335 267 246 181 123 95
90 65 61 34 34 9
BbeI G_GCGC'C
Cuts at: 0 19 50 2424
Size: 19 31 2374
Fragments arranged by size:
2374 31 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 406 1784 2424
Size: 406 1378 640
Fragments arranged by size:
1378 640 406
BbvCI CC'TCA_GC
Cuts at: 0 1978 2093 2394 2424
Size: 1978 115 301 30
Fragments arranged by size:
1978 301 115 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 224 1231 1265 2424
Size: 190 34 1007 34 1159
Fragments arranged by size:
1159 1007 190 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 55 139 385 780 1351 1899 1988
Size: 44 11 84 246 395 571 548 89
Cuts at: 1988 2424
Size: 436
Fragments arranged by size:
571 548 436 395 246 89 84 44
11
BglII A'GATC_T
Cuts at: 0 1409 1475 2424
Size: 1409 66 949
Fragments arranged by size:
1409 949 66
Bme1580I G_kGCm'C
Cuts at: 0 13 95 492 1963 2028 2363 2424
Size: 13 82 397 1471 65 335 61
Fragments arranged by size:
1471 397 335 82 65 61 13
BmgBI CAC'GTC
Cuts at: 0 746 978 2424
Size: 746 232 1446
Fragments arranged by size:
1446 746 232
BmrI ACTGGGnnnn_n'
Cuts at: 0 1032 2424
Size: 1032 1392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 301 374 1159 1471 1953 2039 2424
Size: 104 197 73 785 312 482 86 385
Fragments arranged by size:
785 482 385 312 197 104 86 73
Bpu10I CC'TnA_GC
Cuts at: 0 1978 2093 2394 2424
Size: 1978 115 301 30
Fragments arranged by size:
1978 301 115 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1837 1978 2306 2424
Size: 713 1124 141 328 118
Fragments arranged by size:
1124 713 328 141 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 196 1726 2152 2424
Size: 196 1530 426 272
Fragments arranged by size:
1530 426 272 196
BsaHI Gr'CG_yC
Cuts at: 0 16 47 1603 2424
Size: 16 31 1556 821
Fragments arranged by size:
1556 821 31 16
BsaWI w'CCGG_w
Cuts at: 0 678 708 2424
Size: 678 30 1716
Fragments arranged by size:
1716 678 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 195 225 690 720 724 754 796 826
Size: 195 30 465 30 4 30 42 30
Cuts at: 826 1870 1900 2424
Size: 1044 30 524
Fragments arranged by size:
1044 524 465 195 42 30 30 30
30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 1077 1177 1945 1956 1966 2424
Size: 421 656 100 768 11 10 458
Fragments arranged by size:
768 656 458 421 100 11 10
BseYI C'CCAG_C
Cuts at: 0 225 237 702 1689 1760 1847 2062 2424
Size: 225 12 465 987 71 87 215 362
Fragments arranged by size:
987 465 362 225 215 87 71 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1204 1426 1463 2424
Size: 536 668 222 37 961
Fragments arranged by size:
961 668 536 222 37
BsiEI CG_ry'CG
Cuts at: 0 200 2424
Size: 200 2224
BsiHKAI G_wGCw'C
Cuts at: 0 865 964 998 1553 2424
Size: 865 99 34 555 871
Fragments arranged by size:
871 865 555 99 34
BsmI GAATG_Cn'
Cuts at: 0 1845 2424
Size: 1845 579
Bsp1286I G_dGCh'C
Cuts at: 0 13 95 341 375 492 742 865 874
Size: 13 82 246 34 117 250 123 9
Cuts at: 874 964 998 1179 1553 1696 1963 2028 2363
Size: 90 34 181 374 143 267 65 335
Cuts at: 2363 2424
Size: 61
Fragments arranged by size:
374 335 267 250 246 181 143 123
117 90 82 65 61 34 34 13
9
BspEI T'CCGG_A
Cuts at: 0 708 2424
Size: 708 1716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1226 1532 1763 2424
Size: 1226 306 231 661
Fragments arranged by size:
1226 661 306 231
BsrBI CCG'CTC
Cuts at: 0 1833 2181 2202 2223 2235 2298 2424
Size: 1833 348 21 21 12 63 126
Fragments arranged by size:
1833 348 126 63 21 21 12
BsrFI r'CCGG_y
Cuts at: 0 1313 1809 1937 2424
Size: 1313 496 128 487
Fragments arranged by size:
1313 496 487 128
BssSI C'ACGA_G
Cuts at: 0 1240 1927 2424
Size: 1240 687 497
Fragments arranged by size:
1240 687 497
BstAPI GCAn_nnn'nTGC
Cuts at: 0 559 1770 2424
Size: 559 1211 654
Fragments arranged by size:
1211 654 559
BstEII G'GTnAC_C
Cuts at: 0 1797 2424
Size: 1797 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 1197 1464 2380 2424
Size: 972 225 267 916 44
Fragments arranged by size:
972 916 267 225 44
BstYI r'GATC_y
Cuts at: 0 1079 1409 1475 2424
Size: 1079 330 66 949
Fragments arranged by size:
1079 949 330 66
Bsu36I CC'TnA_GG
Cuts at: 0 540 639 2424
Size: 540 99 1785
Fragments arranged by size:
1785 540 99
BtgI C'CryG_G
Cuts at: 0 53 341 866 971 1349 1736 2424
Size: 53 288 525 105 378 387 688
Fragments arranged by size:
688 525 387 378 288 105 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1501 2424
Size: 1501 923
BtsI GCAGTG_nn'
Cuts at: 0 353 1771 2087 2424
Size: 353 1418 316 337
Fragments arranged by size:
1418 353 337 316
DraIII CAC_nnn'GTG
Cuts at: 0 559 2424
Size: 559 1865
DrdI GACnn_nn'nnGTC
Cuts at: 0 840 2424
Size: 840 1584
EaeI y'GGCC_r
Cuts at: 0 131 197 377 2424
Size: 131 66 180 2047
Fragments arranged by size:
2047 180 131 66
EagI C'GGCC_G
Cuts at: 0 197 2424
Size: 197 2227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 2424
Size: 865 1559
EcoICRI GAG'CTC
Cuts at: 0 863 962 996 2424
Size: 863 99 34 1428
Fragments arranged by size:
1428 863 99 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 301 374 1159 1315 1471 1953 2039
Size: 104 197 73 785 156 156 482 86
Cuts at: 2039 2424
Size: 385
Fragments arranged by size:
785 482 385 197 156 156 104 86
73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 650 2072 2424
Size: 650 1422 352
Fragments arranged by size:
1422 650 352
EcoO109I rG'GnC_Cy
Cuts at: 0 115 186 440 452 464 643 655 757
Size: 115 71 254 12 12 179 12 102
Cuts at: 757 786 833 1185 1215 1278 1344 1913 1960
Size: 29 47 352 30 63 66 569 47
Cuts at: 1960 2024 2025 2077 2360 2368 2390 2424
Size: 64 1 52 283 8 22 34
Fragments arranged by size:
569 352 283 254 179 115 102 71
66 64 63 52 47 47 34 30
29 22 12 12 12 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 939 1671 2424
Size: 939 732 753
Fragments arranged by size:
939 753 732
FspI TGC'GCA
Cuts at: 0 904 2424
Size: 904 1520
HaeII r_GCGC'y
Cuts at: 0 19 50 1938 2424
Size: 19 31 1888 486
Fragments arranged by size:
1888 486 31 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 195 227 622 654 690 722 724 749
Size: 195 32 395 32 36 32 2 25
Cuts at: 749 756 781 847 879 2377 2409 2424
Size: 7 25 66 32 1498 32 15
Fragments arranged by size:
1498 395 195 66 36 32 32 32
32 32 25 25 15 7 2
HindIII A'AGCT_T
Cuts at: 0 2332 2424
Size: 2332 92
KasI G'GCGC_C
Cuts at: 0 15 46 2424
Size: 15 31 2378
Fragments arranged by size:
2378 31 15
MslI CAynn'nnrTG
Cuts at: 0 154 265 970 1372 2378 2424
Size: 154 111 705 402 1006 46
Fragments arranged by size:
1006 705 402 154 111 46
MspA1I CmG'CkG
Cuts at: 0 55 343 990 1464 1509 1746 2017 2424
Size: 55 288 647 474 45 237 271 407
Fragments arranged by size:
647 474 407 288 271 237 55 45
NaeI GCC'GGC
Cuts at: 0 1939 2424
Size: 1939 485
NarI GG'CG_CC
Cuts at: 0 16 47 2424
Size: 16 31 2377
Fragments arranged by size:
2377 31 16
NcoI C'CATG_G
Cuts at: 0 866 1736 2424
Size: 866 870 688
Fragments arranged by size:
870 866 688
NgoMIV G'CCGG_C
Cuts at: 0 1937 2424
Size: 1937 487
NspI r_CATG'y
Cuts at: 0 1014 1377 1629 1728 1773 2424
Size: 1014 363 252 99 45 651
Fragments arranged by size:
1014 651 363 252 99 45
PasI CC'CwG_GG
Cuts at: 0 1598 1956 2074 2424
Size: 1598 358 118 350
Fragments arranged by size:
1598 358 350 118
PciI A'CATG_T
Cuts at: 0 1010 1625 2424
Size: 1010 615 799
Fragments arranged by size:
1010 799 615
PflMI CCAn_nnn'nTGG
Cuts at: 0 1106 1689 2424
Size: 1106 583 735
Fragments arranged by size:
1106 735 583
PfoI T'CCnGG_A
Cuts at: 0 111 2424
Size: 111 2313
PpuMI rG'GwC_Cy
Cuts at: 0 115 186 440 452 464 757 833 1185
Size: 115 71 254 12 12 293 76 352
Cuts at: 1185 1215 1278 1913 2424
Size: 30 63 635 511
Fragments arranged by size:
635 511 352 293 254 115 76 71
63 30 12 12
PspOMI G'GGCC_C
Cuts at: 0 91 1959 2024 2359 2424
Size: 91 1868 65 335 65
Fragments arranged by size:
1868 335 91 65 65
PspXI vC'TCGA_Gb
Cuts at: 0 776 1256 2424
Size: 776 480 1168
Fragments arranged by size:
1168 776 480
PstI C_TGCA'G
Cuts at: 0 1886 2424
Size: 1886 538
PvuII CAG'CTG
Cuts at: 0 1464 1746 2017 2424
Size: 1464 282 271 407
Fragments arranged by size:
1464 407 282 271
SacI G_AGCT'C
Cuts at: 0 865 964 998 2424
Size: 865 99 34 1426
Fragments arranged by size:
1426 865 99 34
SacII CC_GC'GG
Cuts at: 0 56 344 2424
Size: 56 288 2080
Fragments arranged by size:
2080 288 56
SanDI GG'GwC_CC
Cuts at: 0 186 440 452 1185 1913 2424
Size: 186 254 12 733 728 511
Fragments arranged by size:
733 728 511 254 186 12
ScaI AGT'ACT
Cuts at: 0 943 2424
Size: 943 1481
SfcI C'TryA_G
Cuts at: 0 1437 1882 2424
Size: 1437 445 542
Fragments arranged by size:
1437 542 445
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 139 2424
Size: 139 2285
SfoI GGC'GCC
Cuts at: 0 17 48 2424
Size: 17 31 2376
Fragments arranged by size:
2376 31 17
SgrAI Cr'CCGG_yG
Cuts at: 0 1937 2424
Size: 1937 487
SmaI CCC'GGG
Cuts at: 0 76 85 165 191 1918 2011 2424
Size: 76 9 80 26 1727 93 413
Fragments arranged by size:
1727 413 93 80 76 26 9
SmlI C'TyrA_G
Cuts at: 0 728 776 1256 1852 1993 2321 2424
Size: 728 48 480 596 141 328 103
Fragments arranged by size:
728 596 480 328 141 103 48
SphI G_CATG'C
Cuts at: 0 1773 2424
Size: 1773 651
StuI AGG'CCT
Cuts at: 0 286 1300 2424
Size: 286 1014 1124
Fragments arranged by size:
1124 1014 286
StyI C'CwwG_G
Cuts at: 0 836 845 866 927 1719 1736 2141 2424
Size: 836 9 21 61 792 17 405 283
Fragments arranged by size:
836 792 405 283 61 21 17 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2136 2424
Size: 2136 288
TatI w'GTAC_w
Cuts at: 0 941 2424
Size: 941 1483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 518 550 1031 1063 1177 1209
Size: 107 32 379 32 481 32 114 32
Cuts at: 1209 2424
Size: 1215
Fragments arranged by size:
1215 481 379 114 107 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 2424
Size: 750 1674
XhoI C'TCGA_G
Cuts at: 0 776 1256 2424
Size: 776 480 1168
Fragments arranged by size:
1168 776 480
XmaI C'CCGG_G
Cuts at: 0 74 83 163 189 1916 2009 2424
Size: 74 9 80 26 1727 93 415
Fragments arranged by size:
1727 415 93 80 74 26 9
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HindIII KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SanDI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BlpI BmtI BplI BsaAI BsaBI
BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstZ17I
ClaI CspCI DraI EarI EcoRI EcoRV FseI FspAI
HincII HpaI KpnI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SalI SapI SbfI
SexAI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XmnI ZraI