Restriction map for
pF1KE1026
(Linear) (Six-base) MAPSORT of: KIEE1026.seq Check: 7210 from: 1 to: 2937
KIEE1026.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 591 840 2937
Size: 399 192 249 2097
Fragments arranged by size:
2097 399 249 192
AatII G_ACGT'C
Cuts at: 0 1865 2937
Size: 1865 1072
AccI GT'mk_AC
Cuts at: 0 16 1859 2937
Size: 16 1843 1078
Fragments arranged by size:
1843 1078 16
Acc65I G'GTAC_C
Cuts at: 0 592 2937
Size: 592 2345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 2937
Size: 338 2599
AflIII A'CryG_T
Cuts at: 0 470 864 866 1076 2252 2654 2937
Size: 470 394 2 210 1176 402 283
Fragments arranged by size:
1176 470 402 394 283 210 2
AgeI A'CCGG_T
Cuts at: 0 262 2937
Size: 262 2675
AleI CACnn'nnGTG
Cuts at: 0 1237 1888 2937
Size: 1237 651 1049
Fragments arranged by size:
1237 1049 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1801 2937
Size: 1801 1136
AlwNI CAG_nnn'CTG
Cuts at: 0 432 531 693 792 1413 1830 2070 2241
Size: 432 99 162 99 621 417 240 171
Cuts at: 2241 2322 2409 2925 2937
Size: 81 87 516 12
Fragments arranged by size:
621 516 432 417 240 171 162 99
99 87 81 12
ApaI G_GGCC'C
Cuts at: 0 669 1805 2860 2937
Size: 669 1136 1055 77
Fragments arranged by size:
1136 1055 669 77
ApaLI G'TGCA_C
Cuts at: 0 266 604 2937
Size: 266 338 2333
Fragments arranged by size:
2333 338 266
AvaI C'yCGr_G
Cuts at: 0 952 1804 2188 2937
Size: 952 852 384 749
Fragments arranged by size:
952 852 749 384
BanI G'GyrC_C
Cuts at: 0 108 322 418 592 852 1231 1489 1944
Size: 108 214 96 174 260 379 258 455
Cuts at: 1944 2224 2937
Size: 280 713
Fragments arranged by size:
713 455 379 280 260 258 214 174
108 96
BanII G_rGCy'C
Cuts at: 0 181 233 669 1421 1766 1805 2024 2860
Size: 181 52 436 752 345 39 219 836
Cuts at: 2860 2937
Size: 77
Fragments arranged by size:
836 752 436 345 219 181 77 52
39
BbeI G_GCGC'C
Cuts at: 0 1948 2228 2937
Size: 1948 280 709
Fragments arranged by size:
1948 709 280
BbsI GAAGACnn'nnnn_
Cuts at: 0 1452 1912 2937
Size: 1452 460 1025
Fragments arranged by size:
1452 1025 460
BbvCI CC'TCA_GC
Cuts at: 0 149 1346 1927 2937
Size: 149 1197 581 1010
Fragments arranged by size:
1197 1010 581 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1074 1108 2937
Size: 1074 34 1829
Fragments arranged by size:
1829 1074 34
BglI GCCn_nnn'nGGC
Cuts at: 0 331 383 1487 2734 2887 2937
Size: 331 52 1104 1247 153 50
Fragments arranged by size:
1247 1104 331 153 52 50
BglII A'GATC_T
Cuts at: 0 739 2937
Size: 739 2198
BlpI GC'TnA_GC
Cuts at: 0 2323 2937
Size: 2323 614
Bme1580I G_kGCm'C
Cuts at: 0 270 421 506 608 669 679 857 1492
Size: 270 151 85 102 61 10 178 635
Cuts at: 1492 1805 2860 2937
Size: 313 1055 77
Fragments arranged by size:
1055 635 313 270 178 151 102 85
77 61 10
BmgBI CAC'GTC
Cuts at: 0 2094 2255 2937
Size: 2094 161 682
Fragments arranged by size:
2094 682 161
BmrI ACTGGGnnnn_n'
Cuts at: 0 1124 1931 2105 2937
Size: 1124 807 174 832
Fragments arranged by size:
1124 832 807 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2385 2937
Size: 2385 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 793 829 858 1435 1756 1935 2173
Size: 466 327 36 29 577 321 179 238
Cuts at: 2173 2431 2473 2890 2916 2937
Size: 258 42 417 26 21
Fragments arranged by size:
577 466 417 327 321 258 238 179
42 36 29 26 21
Bpu10I CC'TnA_GC
Cuts at: 0 149 1346 1927 2937
Size: 149 1197 581 1010
Fragments arranged by size:
1197 1010 581 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1603 2482 2536 2937
Size: 812 791 879 54 401
Fragments arranged by size:
879 812 791 401 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 579 2692 2857 2937
Size: 579 2113 165 80
Fragments arranged by size:
2113 579 165 80
BsaAI yAC'GTr
Cuts at: 0 222 471 822 867 1077 1239 2937
Size: 222 249 351 45 210 162 1698
Fragments arranged by size:
1698 351 249 222 210 162 45
BsaHI Gr'CG_yC
Cuts at: 0 707 958 1796 1862 1945 2225 2937
Size: 707 251 838 66 83 280 712
Fragments arranged by size:
838 712 707 280 251 83 66
BsaWI w'CCGG_w
Cuts at: 0 262 1207 2132 2937
Size: 262 945 925 805
Fragments arranged by size:
945 925 805 262
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 856 886 1417 1447 1885 1915 2937
Size: 856 30 531 30 438 30 1022
Fragments arranged by size:
1022 856 531 438 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1996 2206 2539 2788 2800 2803 2806 2937
Size: 1996 210 333 249 12 3 3 131
Fragments arranged by size:
1996 333 249 210 131 12 3 3
BseYI C'CCAG_C
Cuts at: 0 47 74 80 102 113 182 290 422
Size: 47 27 6 22 11 69 108 132
Cuts at: 422 506 596 1120 1260 2853 2937
Size: 84 90 524 140 1593 84
Fragments arranged by size:
1593 524 140 132 108 90 84 84
69 47 27 22 11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 433 472 559 652 805 820 856
Size: 269 164 39 87 93 153 15 36
Cuts at: 856 904 1378 1729 2377 2422 2937
Size: 48 474 351 648 45 515
Fragments arranged by size:
648 515 474 351 269 164 153 93
87 48 45 39 36 15
BsiHKAI G_wGCw'C
Cuts at: 0 233 270 608 1372 1681 1766 2006 2030
Size: 233 37 338 764 309 85 240 24
Cuts at: 2030 2498 2675 2937
Size: 468 177 262
Fragments arranged by size:
764 468 338 309 262 240 233 177
85 37 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 949 1333 1888 2937
Size: 949 384 555 1049
Fragments arranged by size:
1049 949 555 384
Bsp1286I G_dGCh'C
Cuts at: 0 181 233 270 421 506 608 669 679
Size: 181 52 37 151 85 102 61 10
Cuts at: 679 857 1372 1421 1492 1681 1766 1805 2006
Size: 178 515 49 71 189 85 39 201
Cuts at: 2006 2024 2030 2498 2675 2860 2937
Size: 18 6 468 177 185 77
Fragments arranged by size:
515 468 201 189 185 181 178 177
151 102 85 85 77 71 61 52
49 39 37 18 10 6
BspEI T'CCGG_A
Cuts at: 0 2132 2937
Size: 2132 805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 591 687 840 1883 2937
Size: 399 192 96 153 1043 1054
Fragments arranged by size:
1054 1043 399 192 153 96
BsrBI CCG'CTC
Cuts at: 0 1454 2937
Size: 1454 1483
BsrFI r'CCGG_y
Cuts at: 0 262 382 627 2518 2877 2937
Size: 262 120 245 1891 359 60
Fragments arranged by size:
1891 359 262 245 120 60
BsrGI T'GTAC_A
Cuts at: 0 965 1959 2937
Size: 965 994 978
Fragments arranged by size:
994 978 965
BssHII G'CGCG_C
Cuts at: 0 2281 2283 2902 2937
Size: 2281 2 619 35
Fragments arranged by size:
2281 619 35 2
BssSI C'ACGA_G
Cuts at: 0 2370 2937
Size: 2370 567
BstAPI GCAn_nnn'nTGC
Cuts at: 0 432 2241 2925 2937
Size: 432 1809 684 12
Fragments arranged by size:
1809 684 432 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1519 1656 2151 2937
Size: 1519 137 495 786
Fragments arranged by size:
1519 786 495 137
BstYI r'GATC_y
Cuts at: 0 739 2937
Size: 739 2198
BstZ17I GTA'TAC
Cuts at: 0 17 2937
Size: 17 2920
Bsu36I CC'TnA_GG
Cuts at: 0 1790 2937
Size: 1790 1147
BtgI C'CryG_G
Cuts at: 0 588 671 702 1310 1363 2174 2216 2573
Size: 588 83 31 608 53 811 42 357
Cuts at: 2573 2937
Size: 364
Fragments arranged by size:
811 608 588 364 357 83 53 42
31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 917 2691 2937
Size: 917 1774 246
Fragments arranged by size:
1774 917 246
BtsI GCAGTG_nn'
Cuts at: 0 1062 1158 1203 1272 1395 2239 2464 2937
Size: 1062 96 45 69 123 844 225 473
Fragments arranged by size:
1062 844 473 225 123 96 69 45
DraIII CAC_nnn'GTG
Cuts at: 0 205 2937
Size: 205 2732
EaeI y'GGCC_r
Cuts at: 0 207 567 917 1010 1049 1580 2213 2735
Size: 207 360 350 93 39 531 633 522
Cuts at: 2735 2879 2937
Size: 144 58
Fragments arranged by size:
633 531 522 360 350 207 144 93
58 39
EarI CTCTTCn'nnn_
Cuts at: 0 521 651 2548 2777 2917 2937
Size: 521 130 1897 229 140 20
Fragments arranged by size:
1897 521 229 140 130 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1095 2937
Size: 1095 1842
EcoICRI GAG'CTC
Cuts at: 0 231 1764 2937
Size: 231 1533 1173
Fragments arranged by size:
1533 1173 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 466 793 829 858 1435 1756 1935
Size: 338 128 327 36 29 577 321 179
Cuts at: 1935 2173 2431 2473 2890 2916 2937
Size: 238 258 42 417 26 21
Fragments arranged by size:
577 417 338 327 321 258 238 179
128 42 36 29 26 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1914 2272 2937
Size: 1914 358 665
Fragments arranged by size:
1914 665 358
EcoO109I rG'GnC_Cy
Cuts at: 0 60 665 666 804 1827 2067 2586 2937
Size: 60 605 1 138 1023 240 519 351
Fragments arranged by size:
1023 605 519 351 240 138 60 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2577 2937
Size: 2577 360
FspI TGC'GCA
Cuts at: 0 1528 2599 2937
Size: 1528 1071 338
Fragments arranged by size:
1528 1071 338
HaeII r_GCGC'y
Cuts at: 0 769 1703 1948 2228 2250 2519 2606 2926
Size: 769 934 245 280 22 269 87 320
Cuts at: 2926 2937
Size: 11
Fragments arranged by size:
934 769 320 280 269 245 87 22
11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 731 763 2937
Size: 331 32 368 32 2174
Fragments arranged by size:
2174 368 331 32 32
HincII GTy'rAC
Cuts at: 0 1425 1860 2181 2937
Size: 1425 435 321 756
Fragments arranged by size:
1425 756 435 321
KasI G'GCGC_C
Cuts at: 0 1944 2224 2937
Size: 1944 280 713
Fragments arranged by size:
1944 713 280
KpnI G_GTAC'C
Cuts at: 0 596 2937
Size: 596 2341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1670 2937
Size: 329 1341 1267
Fragments arranged by size:
1341 1267 329
MscI TGG'CCA
Cuts at: 0 209 569 1051 1582 2737 2937
Size: 209 360 482 531 1155 200
Fragments arranged by size:
1155 531 482 360 209 200
MslI CAynn'nnrTG
Cuts at: 0 499 1069 1075 1237 1517 1582 1888 1999
Size: 499 570 6 162 280 65 306 111
Cuts at: 1999 2937
Size: 938
Fragments arranged by size:
938 570 499 306 280 162 111 65
6
MspA1I CmG'CkG
Cuts at: 0 106 136 429 486 693 792 963 1139
Size: 106 30 293 57 207 99 171 176
Cuts at: 1139 1223 1953 2218 2384 2406 2937
Size: 84 730 265 166 22 531
Fragments arranged by size:
730 531 293 265 207 176 171 166
106 99 84 57 30 22
NaeI GCC'GGC
Cuts at: 0 2520 2879 2937
Size: 2520 359 58
Fragments arranged by size:
2520 359 58
NarI GG'CG_CC
Cuts at: 0 1945 2225 2937
Size: 1945 280 712
Fragments arranged by size:
1945 712 280
NcoI C'CATG_G
Cuts at: 0 588 2174 2573 2937
Size: 588 1586 399 364
Fragments arranged by size:
1586 588 399 364
NgoMIV G'CCGG_C
Cuts at: 0 2518 2877 2937
Size: 2518 359 60
Fragments arranged by size:
2518 359 60
NspI r_CATG'y
Cuts at: 0 1260 2658 2937
Size: 1260 1398 279
Fragments arranged by size:
1398 1260 279
PciI A'CATG_T
Cuts at: 0 2654 2937
Size: 2654 283
PflMI CCAn_nnn'nTGG
Cuts at: 0 205 1047 1635 2070 2175 2937
Size: 205 842 588 435 105 762
Fragments arranged by size:
842 762 588 435 205 105
PfoI T'CCnGG_A
Cuts at: 0 1325 2582 2937
Size: 1325 1257 355
Fragments arranged by size:
1325 1257 355
PmlI CAC'GTG
Cuts at: 0 471 822 867 2937
Size: 471 351 45 2070
Fragments arranged by size:
2070 471 351 45
PpuMI rG'GwC_Cy
Cuts at: 0 1827 2586 2937
Size: 1827 759 351
Fragments arranged by size:
1827 759 351
PspOMI G'GGCC_C
Cuts at: 0 665 1801 2856 2937
Size: 665 1136 1055 81
Fragments arranged by size:
1136 1055 665 81
PstI C_TGCA'G
Cuts at: 0 176 698 1880 2297 2438 2937
Size: 176 522 1182 417 141 499
Fragments arranged by size:
1182 522 499 417 176 141
PvuII CAG'CTG
Cuts at: 0 486 693 2937
Size: 486 207 2244
Fragments arranged by size:
2244 486 207
SacI G_AGCT'C
Cuts at: 0 233 1766 2937
Size: 233 1533 1171
Fragments arranged by size:
1533 1171 233
SacII CC_GC'GG
Cuts at: 0 2219 2937
Size: 2219 718
SalI G'TCGA_C
Cuts at: 0 1858 2937
Size: 1858 1079
SapI GCTCTTCn'nnn_
Cuts at: 0 521 651 2548 2937
Size: 521 130 1897 389
Fragments arranged by size:
1897 521 389 130
SfcI C'TryA_G
Cuts at: 0 25 172 694 1876 2293 2434 2565 2937
Size: 25 147 522 1182 417 141 131 372
Fragments arranged by size:
1182 522 417 372 147 141 131 25
SfoI GGC'GCC
Cuts at: 0 1946 2226 2937
Size: 1946 280 711
Fragments arranged by size:
1946 711 280
SgrAI Cr'CCGG_yG
Cuts at: 0 627 2937
Size: 627 2310
SmaI CCC'GGG
Cuts at: 0 1806 2937
Size: 1806 1131
SmlI C'TyrA_G
Cuts at: 0 827 1618 2497 2551 2937
Size: 827 791 879 54 386
Fragments arranged by size:
879 827 791 386 54
StuI AGG'CCT
Cuts at: 0 2368 2937
Size: 2368 569
StyI C'CwwG_G
Cuts at: 0 588 1245 2174 2528 2573 2690 2937
Size: 588 657 929 354 45 117 247
Fragments arranged by size:
929 657 588 354 247 117 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 285 436 745 1030 2182 2364 2937
Size: 144 141 151 309 285 1152 182 573
Fragments arranged by size:
1152 573 309 285 182 151 144 141
TatI w'GTAC_w
Cuts at: 0 296 869 965 1959 2651 2657 2937
Size: 296 573 96 994 692 6 280
Fragments arranged by size:
994 692 573 296 280 96 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 856 888 1885 1917 2937
Size: 856 32 997 32 1020
Fragments arranged by size:
1020 997 856 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 345 2937
Size: 345 2592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1632 1995 2712 2937
Size: 528 1104 363 717 225
Fragments arranged by size:
1104 717 528 363 225
XmaI C'CCGG_G
Cuts at: 0 1804 2937
Size: 1804 1133
XmnI GAAnn'nnTTC
Cuts at: 0 1909 2937
Size: 1909 1028
ZraI GAC'GTC
Cuts at: 0 1863 2937
Size: 1863 1074
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SalI SapI SfcI SfoI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmtI
BsaBI BsiEI BsiWI BsmI BspHI BsrDI BstBI BstEII
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SanDI SbfI ScaI SexAI
SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI