Restriction map for
pF1KE1027
(Linear) (Six-base) MAPSORT of: KIEE1027.seq Check: 1160 from: 1 to: 3105
KIEE1027.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 830 1042 1289 3105
Size: 830 212 247 1816
Fragments arranged by size:
1816 830 247 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1672 2236 2590 2686 3105
Size: 863 809 564 354 96 419
Fragments arranged by size:
863 809 564 419 354 96
AhdI GACnn_n'nnGTC
Cuts at: 0 2015 3105
Size: 2015 1090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2209 3105
Size: 2209 896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 580 612 1327 1359 3105
Size: 175 32 373 32 715 32 1746
Fragments arranged by size:
1746 715 373 175 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 228 984 1371 1560 1668 1695 1884 2304
Size: 228 756 387 189 108 27 189 420
Cuts at: 2304 2727 3105
Size: 423 378
Fragments arranged by size:
756 423 420 387 378 228 189 189
108 27
ApaI G_GGCC'C
Cuts at: 0 45 656 955 1129 1610 2391 3105
Size: 45 611 299 174 481 781 714
Fragments arranged by size:
781 714 611 481 299 174 45
ApoI r'AATT_y
Cuts at: 0 751 2050 2857 3105
Size: 751 1299 807 248
Fragments arranged by size:
1299 807 751 248
AvaI C'yCGr_G
Cuts at: 0 344 446 1219 2372 2384 2992 3105
Size: 344 102 773 1153 12 608 113
Fragments arranged by size:
1153 773 608 344 113 102 12
BamHI G'GATC_C
Cuts at: 0 1205 3105
Size: 1205 1900
BanI G'GyrC_C
Cuts at: 0 337 830 881 910 1914 3105
Size: 337 493 51 29 1004 1191
Fragments arranged by size:
1191 1004 493 337 51 29
BanII G_rGCy'C
Cuts at: 0 45 656 872 955 965 1129 1178 1231
Size: 45 611 216 83 10 164 49 53
Cuts at: 1231 1610 2078 2391 2519 3105
Size: 379 468 313 128 586
Fragments arranged by size:
611 586 468 379 313 216 164 128
83 53 49 45 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 2320 3105
Size: 2320 785
BbvCI CC'TCA_GC
Cuts at: 0 298 1950 3105
Size: 298 1652 1155
Fragments arranged by size:
1652 1155 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2967 3001 3105
Size: 2967 34 104
Fragments arranged by size:
2967 104 34
BclI T'GATC_A
Cuts at: 0 1571 3105
Size: 1571 1534
BglI GCCn_nnn'nGGC
Cuts at: 0 33 928 1678 2965 3105
Size: 33 895 750 1287 140
Fragments arranged by size:
1287 895 750 140 33
BglII A'GATC_T
Cuts at: 0 1251 3105
Size: 1251 1854
BlpI GC'TnA_GC
Cuts at: 0 1405 2737 2935 3105
Size: 1405 1332 198 170
Fragments arranged by size:
1405 1332 198 170
Bme1580I G_kGCm'C
Cuts at: 0 45 656 913 955 1129 1595 1610 2252
Size: 45 611 257 42 174 466 15 642
Cuts at: 2252 2391 2431 2765 3024 3105
Size: 139 40 334 259 81
Fragments arranged by size:
642 611 466 334 259 257 174 139
81 45 42 40 15
BmgBI CAC'GTC
Cuts at: 0 2760 3105
Size: 2760 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 2703 3105
Size: 2703 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 657 1276 1699 1831 2020 2344 2356
Size: 256 401 619 423 132 189 324 12
Cuts at: 2356 2896 3105
Size: 540 209
Fragments arranged by size:
619 540 423 401 324 256 209 189
132 12
Bpu10I CC'TnA_GC
Cuts at: 0 37 298 1950 2077 3105
Size: 37 261 1652 127 1028
Fragments arranged by size:
1652 1028 261 127 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 2767 3105
Size: 928 1839 338
Fragments arranged by size:
1839 928 338
BsaAI yAC'GTr
Cuts at: 0 2193 3105
Size: 2193 912
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 1078 1108 1327 1357 3105
Size: 580 30 468 30 219 30 1748
Fragments arranged by size:
1748 580 468 219 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 553 631 634 637 640 788 805
Size: 265 288 78 3 3 3 148 17
Cuts at: 805 868 988 1018 1021 1024 1048 1070 1102
Size: 63 120 30 3 3 24 22 32
Cuts at: 1102 1221 1348 1351 2131 2158 2179 3105
Size: 119 127 3 780 27 21 926
Fragments arranged by size:
926 780 288 265 148 127 120 119
78 63 32 30 27 24 22 21
17 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 470 1179 1560 1626 2260 2461 3043 3105
Size: 470 709 381 66 634 201 582 62
Fragments arranged by size:
709 634 582 470 381 201 66 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2338 2431 2713 3016 3049 3105
Size: 2338 93 282 303 33 56
Fragments arranged by size:
2338 303 282 93 56 33
BsiEI CG_ry'CG
Cuts at: 0 381 672 1187 3105
Size: 381 291 515 1918
Fragments arranged by size:
1918 515 381 291
BsiHKAI G_wGCw'C
Cuts at: 0 525 569 2078 3105
Size: 525 44 1509 1027
Fragments arranged by size:
1509 1027 525 44
BsmI GAATG_Cn'
Cuts at: 0 1831 2681 3105
Size: 1831 850 424
Fragments arranged by size:
1831 850 424
BsmBI CGTCTCn'nnnn_
Cuts at: 0 656 3105
Size: 656 2449
Bsp1286I G_dGCh'C
Cuts at: 0 45 525 569 656 872 913 955 965
Size: 45 480 44 87 216 41 42 10
Cuts at: 965 1129 1178 1231 1595 1610 2078 2252 2391
Size: 164 49 53 364 15 468 174 139
Cuts at: 2391 2431 2519 2765 3024 3105
Size: 40 88 246 259 81
Fragments arranged by size:
480 468 364 259 246 216 174 164
139 88 87 81 53 49 45 44
42 41 40 15 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 830 1042 1289 1892 3105
Size: 830 212 247 603 1213
Fragments arranged by size:
1213 830 603 247 212
BsrBI CCG'CTC
Cuts at: 0 575 707 1404 1647 2346 2540 2880 3105
Size: 575 132 697 243 699 194 340 225
Fragments arranged by size:
699 697 575 340 243 225 194 132
BsrFI r'CCGG_y
Cuts at: 0 1501 1901 3105
Size: 1501 400 1204
Fragments arranged by size:
1501 1204 400
BssHII G'CGCG_C
Cuts at: 0 1748 3105
Size: 1748 1357
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2727 3105
Size: 2727 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3051 3105
Size: 3051 54
BstYI r'GATC_y
Cuts at: 0 1205 1251 1454 3070 3105
Size: 1205 46 203 1616 35
Fragments arranged by size:
1616 1205 203 46 35
Bsu36I CC'TnA_GG
Cuts at: 0 971 1046 1082 3105
Size: 971 75 36 2023
Fragments arranged by size:
2023 971 75 36
BtgI C'CryG_G
Cuts at: 0 197 375 381 1410 1673 2556 2744 2765
Size: 197 178 6 1029 263 883 188 21
Cuts at: 2765 3105
Size: 340
Fragments arranged by size:
1029 883 340 263 197 188 178 21
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 437 1556 3105
Size: 437 1119 1549
Fragments arranged by size:
1549 1119 437
BtsI GCAGTG_nn'
Cuts at: 0 217 1072 1111 1391 1519 1621 2429 3105
Size: 217 855 39 280 128 102 808 676
Fragments arranged by size:
855 808 676 280 217 128 102 39
DraIII CAC_nnn'GTG
Cuts at: 0 529 2409 2711 3105
Size: 529 1880 302 394
Fragments arranged by size:
1880 529 394 302
EaeI y'GGCC_r
Cuts at: 0 378 1184 1670 2291 3105
Size: 378 806 486 621 814
Fragments arranged by size:
814 806 621 486 378
EagI C'GGCC_G
Cuts at: 0 378 1184 3105
Size: 378 806 1921
Fragments arranged by size:
1921 806 378
EarI CTCTTCn'nnn_
Cuts at: 0 167 782 2010 2090 2165 2648 3105
Size: 167 615 1228 80 75 483 457
Fragments arranged by size:
1228 615 483 457 167 80 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 672 1309 3105
Size: 672 637 1796
Fragments arranged by size:
1796 672 637
EcoICRI GAG'CTC
Cuts at: 0 2076 3105
Size: 2076 1029
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 657 863 1276 1672 1699 1831 2020
Size: 256 401 206 413 396 27 132 189
Cuts at: 2020 2236 2344 2356 2590 2686 2896 3105
Size: 216 108 12 234 96 210 209
Fragments arranged by size:
413 401 396 256 234 216 210 209
206 189 132 108 96 27 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 1022 1049 1285 2785 3105
Size: 604 418 27 236 1500 320
Fragments arranged by size:
1500 604 418 320 236 27
EcoO109I rG'GnC_Cy
Cuts at: 0 41 42 165 428 652 653 929 951
Size: 41 1 123 263 224 1 276 22
Cuts at: 951 952 1042 1368 1440 1462 1606 1607 1776
Size: 1 90 326 72 22 144 1 169
Cuts at: 1776 2394 2421 2690 3105
Size: 618 27 269 415
Fragments arranged by size:
618 415 326 276 269 263 224 169
144 123 90 72 41 27 22 22
1 1 1 1
HaeII r_GCGC'y
Cuts at: 0 597 681 841 1855 2127 2744 2945 3105
Size: 597 84 160 1014 272 617 201 160
Fragments arranged by size:
1014 617 597 272 201 160 160 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 2001 2033 2779 2811 3105
Size: 1078 32 891 32 746 32 294
Fragments arranged by size:
1078 891 746 294 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 3105
Size: 980 2125
MscI TGG'CCA
Cuts at: 0 2293 3105
Size: 2293 812
MspA1I CmG'CkG
Cuts at: 0 199 243 377 392 635 674 1183 1212
Size: 199 44 134 15 243 39 509 29
Cuts at: 1212 1560 1675 1695 1719 2040 2334 2352 2507
Size: 348 115 20 24 321 294 18 155
Cuts at: 2507 2910 3105
Size: 403 195
Fragments arranged by size:
509 403 348 321 294 243 199 195
155 134 115 44 39 29 24 20
18 15
NaeI GCC'GGC
Cuts at: 0 1503 3105
Size: 1503 1602
NcoI C'CATG_G
Cuts at: 0 1410 2765 3105
Size: 1410 1355 340
Fragments arranged by size:
1410 1355 340
NgoMIV G'CCGG_C
Cuts at: 0 1501 3105
Size: 1501 1604
PasI CC'CwG_GG
Cuts at: 0 1122 1365 1603 3105
Size: 1122 243 238 1502
Fragments arranged by size:
1502 1122 243 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 999 1560 1985 3105
Size: 999 561 425 1120
Fragments arranged by size:
1120 999 561 425
PfoI T'CCnGG_A
Cuts at: 0 167 2054 2321 2873 3105
Size: 167 1887 267 552 232
Fragments arranged by size:
1887 552 267 232 167
PmlI CAC'GTG
Cuts at: 0 2193 3105
Size: 2193 912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1327 1359 3105
Size: 580 32 715 32 1746
Fragments arranged by size:
1746 715 580 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 165 1440 1776 3105
Size: 165 1275 336 1329
Fragments arranged by size:
1329 1275 336 165
PspOMI G'GGCC_C
Cuts at: 0 41 652 951 1125 1606 2387 3105
Size: 41 611 299 174 481 781 718
Fragments arranged by size:
781 718 611 481 299 174 41
PstI C_TGCA'G
Cuts at: 0 182 209 224 1167 1286 1319 1386 1700
Size: 182 27 15 943 119 33 67 314
Cuts at: 1700 1841 2678 2954 3105
Size: 141 837 276 151
Fragments arranged by size:
943 837 314 276 182 151 141 119
67 33 27 15
PvuII CAG'CTG
Cuts at: 0 392 635 1212 1695 1719 2040 2334 2352
Size: 392 243 577 483 24 321 294 18
Cuts at: 2352 3105
Size: 753
Fragments arranged by size:
753 577 483 392 321 294 243 24
18
SacI G_AGCT'C
Cuts at: 0 2078 3105
Size: 2078 1027
SacII CC_GC'GG
Cuts at: 0 200 378 1676 3105
Size: 200 178 1298 1429
Fragments arranged by size:
1429 1298 200 178
SapI GCTCTTCn'nnn_
Cuts at: 0 167 2165 3105
Size: 167 1998 940
Fragments arranged by size:
1998 940 167
SbfI CC_TGCA'GG
Cuts at: 0 1319 3105
Size: 1319 1786
SfcI C'TryA_G
Cuts at: 0 178 205 220 1163 1282 1315 1382 1696
Size: 178 27 15 943 119 33 67 314
Cuts at: 1696 1837 2224 2674 2771 2950 3105
Size: 141 387 450 97 179 155
Fragments arranged by size:
943 450 387 314 179 178 155 141
119 97 67 33 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1678 2965 3105
Size: 1678 1287 140
Fragments arranged by size:
1678 1287 140
SmaI CCC'GGG
Cuts at: 0 448 1221 2994 3105
Size: 448 773 1773 111
Fragments arranged by size:
1773 773 448 111
SmlI C'TyrA_G
Cuts at: 0 943 2782 3105
Size: 943 1839 323
Fragments arranged by size:
1839 943 323
StyI C'CwwG_G
Cuts at: 0 556 956 993 1328 1410 1458 2765 3105
Size: 556 400 37 335 82 48 1307 340
Fragments arranged by size:
1307 556 400 340 335 82 48 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2274 2439 2722 3105
Size: 2274 165 283 383
Fragments arranged by size:
2274 383 283 165
XbaI T'CTAG_A
Cuts at: 0 2025 3105
Size: 2025 1080
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 1521 2751 2871 3105
Size: 102 1419 1230 120 234
Fragments arranged by size:
1419 1230 234 120 102
XmaI C'CCGG_G
Cuts at: 0 446 1219 2992 3105
Size: 446 773 1773 113
Fragments arranged by size:
1773 773 446 113
XmnI GAAnn'nnTTC
Cuts at: 0 1429 3105
Size: 1429 1676
Enzymes that do cut:
AarI AcuI AhdI AlfI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MscI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SapI SbfI SfcI SfiI SmaI SmlI
StyI TaqII XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BciVI BfrBI BmtI BplI BsaI BsaBI BsaHI BsaWI
BsiWI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XhoI ZraI