Restriction map for
pF1KE1028
(Linear) (Six-base) MAPSORT of: KIEE1028.seq Check: 5092 from: 1 to: 3651
KIEE1028.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3 3651
Size: 3 3648
AccI GT'mk_AC
Cuts at: 0 2314 3651
Size: 2314 1337
AclI AA'CG_TT
Cuts at: 0 972 3651
Size: 972 2679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1289 2404 2674 3102 3651
Size: 51 1238 1115 270 428 549
Fragments arranged by size:
1238 1115 549 428 270 51
AfeI AGC'GCT
Cuts at: 0 381 2707 3651
Size: 381 2326 944
Fragments arranged by size:
2326 944 381
AflIII A'CryG_T
Cuts at: 0 1872 3651
Size: 1872 1779
AgeI A'CCGG_T
Cuts at: 0 260 3651
Size: 260 3391
AleI CACnn'nnGTG
Cuts at: 0 1645 3651
Size: 1645 2006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1463 3651
Size: 1463 2188
AlwNI CAG_nnn'CTG
Cuts at: 0 996 2674 2877 3444 3651
Size: 996 1678 203 567 207
Fragments arranged by size:
1678 996 567 207 203
ApoI r'AATT_y
Cuts at: 0 839 1048 2179 2825 2973 3553 3651
Size: 839 209 1131 646 148 580 98
Fragments arranged by size:
1131 839 646 580 209 148 98
AseI AT'TA_AT
Cuts at: 0 1217 2120 3651
Size: 1217 903 1531
Fragments arranged by size:
1531 1217 903
AvaI C'yCGr_G
Cuts at: 0 1337 2477 3651
Size: 1337 1140 1174
Fragments arranged by size:
1337 1174 1140
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 482 515 3651
Size: 482 33 3136
Fragments arranged by size:
3136 482 33
BanI G'GyrC_C
Cuts at: 0 307 3651
Size: 307 3344
BanII G_rGCy'C
Cuts at: 0 666 3651
Size: 666 2985
BbsI GAAGACnn'nnnn_
Cuts at: 0 1268 3651
Size: 1268 2383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 722 3651
Size: 688 34 2929
Fragments arranged by size:
2929 688 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1782 3651
Size: 1782 1869
BclI T'GATC_A
Cuts at: 0 1826 2133 3599 3651
Size: 1826 307 1466 52
Fragments arranged by size:
1826 1466 307 52
BglII A'GATC_T
Cuts at: 0 1464 3651
Size: 1464 2187
BlpI GC'TnA_GC
Cuts at: 0 731 776 3452 3651
Size: 731 45 2676 199
Fragments arranged by size:
2676 731 199 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 2003 2350 3651
Size: 2003 347 1301
Fragments arranged by size:
2003 1301 347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1766 3302 3651
Size: 1312 454 1536 349
Fragments arranged by size:
1536 1312 454 349
Bpu10I CC'TnA_GC
Cuts at: 0 2264 3651
Size: 2264 1387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1180 2926 3185 3559 3651
Size: 1082 98 1746 259 374 92
Fragments arranged by size:
1746 1082 374 259 98 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 2158 3651
Size: 2158 1493
BsaWI w'CCGG_w
Cuts at: 0 260 3651
Size: 260 3391
BseYI C'CCAG_C
Cuts at: 0 1919 2168 3651
Size: 1919 249 1483
Fragments arranged by size:
1919 1483 249
BsiHKAI G_wGCw'C
Cuts at: 0 1989 3651
Size: 1989 1662
BsmI GAATG_Cn'
Cuts at: 0 1434 3651
Size: 1434 2217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 651 3651
Size: 651 3000
Bsp1286I G_dGCh'C
Cuts at: 0 666 1989 3651
Size: 666 1323 1662
Fragments arranged by size:
1662 1323 666
BspHI T'CATG_A
Cuts at: 0 2748 3651
Size: 2748 903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 2105 3651
Size: 3 2102 1546
Fragments arranged by size:
2102 1546 3
BsrFI r'CCGG_y
Cuts at: 0 260 3651
Size: 260 3391
BsrGI T'GTAC_A
Cuts at: 0 2 3495 3651
Size: 2 3493 156
Fragments arranged by size:
3493 156 2
BssSI C'ACGA_G
Cuts at: 0 212 704 3651
Size: 212 492 2947
Fragments arranged by size:
2947 492 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 996 1450 2790 3651
Size: 996 454 1340 861
Fragments arranged by size:
1340 996 861 454
BstBI TT'CG_AA
Cuts at: 0 33 294 2898 3651
Size: 33 261 2604 753
Fragments arranged by size:
2604 753 261 33
BstEII G'GTnAC_C
Cuts at: 0 263 1963 3651
Size: 263 1700 1688
Fragments arranged by size:
1700 1688 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3280 3651
Size: 3280 371
BstYI r'GATC_y
Cuts at: 0 60 103 1119 1317 1464 3651
Size: 60 43 1016 198 147 2187
Fragments arranged by size:
2187 1016 198 147 60 43
BtsI GCAGTG_nn'
Cuts at: 0 1655 2917 3261 3651
Size: 1655 1262 344 390
Fragments arranged by size:
1655 1262 390 344
ClaI AT'CG_AT
Cuts at: 0 272 3037 3651
Size: 272 2765 614
Fragments arranged by size:
2765 614 272
DraI TTT'AAA
Cuts at: 0 1517 2184 3651
Size: 1517 667 1467
Fragments arranged by size:
1517 1467 667
EaeI y'GGCC_r
Cuts at: 0 3614 3651
Size: 3614 37
EarI CTCTTCn'nnn_
Cuts at: 0 291 584 1418 2227 2377 2689 2804 3008
Size: 291 293 834 809 150 312 115 204
Cuts at: 3008 3651
Size: 643
Fragments arranged by size:
834 809 643 312 293 291 204 150
115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1289 1312 1766 2404 2674 3102 3302
Size: 51 1238 23 454 638 270 428 200
Cuts at: 3302 3651
Size: 349
Fragments arranged by size:
1238 638 454 428 349 270 200 51
23
EcoRV GAT'ATC
Cuts at: 0 807 3651
Size: 807 2844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 307 419 886 3651
Size: 307 112 467 2765
Fragments arranged by size:
2765 467 307 112
FspI TGC'GCA
Cuts at: 0 2101 3651
Size: 2101 1550
HaeII r_GCGC'y
Cuts at: 0 383 2709 3651
Size: 383 2326 942
Fragments arranged by size:
2326 942 383
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1830 1862 2763 2795 3651
Size: 1830 32 901 32 856
Fragments arranged by size:
1830 901 856 32 32
HincII GTy'rAC
Cuts at: 0 2416 2946 3092 3270 3651
Size: 2416 530 146 178 381
Fragments arranged by size:
2416 530 381 178 146
HindIII A'AGCT_T
Cuts at: 0 1862 2295 3651
Size: 1862 433 1356
Fragments arranged by size:
1862 1356 433
HpaI GTT'AAC
Cuts at: 0 2946 3092 3651
Size: 2946 146 559
Fragments arranged by size:
2946 559 146
MfeI C'AATT_G
Cuts at: 0 53 3651
Size: 53 3598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 3267 3651
Size: 893 2374 384
Fragments arranged by size:
2374 893 384
MscI TGG'CCA
Cuts at: 0 3616 3651
Size: 3616 35
MslI CAynn'nnrTG
Cuts at: 0 1645 1982 2735 3593 3651
Size: 1645 337 753 858 58
Fragments arranged by size:
1645 858 753 337 58
MspA1I CmG'CkG
Cuts at: 0 180 1923 3547 3651
Size: 180 1743 1624 104
Fragments arranged by size:
1743 1624 180 104
NdeI CA'TA_TG
Cuts at: 0 2732 3651
Size: 2732 919
NspI r_CATG'y
Cuts at: 0 1582 1646 1876 2305 2793 3502 3651
Size: 1582 64 230 429 488 709 149
Fragments arranged by size:
1582 709 488 429 230 149 64
PciI A'CATG_T
Cuts at: 0 1872 3651
Size: 1872 1779
PflMI CCAn_nnn'nTGG
Cuts at: 0 3444 3651
Size: 3444 207
PfoI T'CCnGG_A
Cuts at: 0 1743 3651
Size: 1743 1908
PspXI vC'TCGA_Gb
Cuts at: 0 1337 2477 3651
Size: 1337 1140 1174
Fragments arranged by size:
1337 1174 1140
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3479 3511 3651
Size: 3479 32 140
Fragments arranged by size:
3479 140 32
PvuII CAG'CTG
Cuts at: 0 1923 3547 3651
Size: 1923 1624 104
Fragments arranged by size:
1923 1624 104
SexAI A'CCwGG_T
Cuts at: 0 3144 3651
Size: 3144 507
SmlI C'TyrA_G
Cuts at: 0 1097 1195 1337 2477 2905 3200 3538 3651
Size: 1097 98 142 1140 428 295 338 113
Fragments arranged by size:
1140 1097 428 338 295 142 113 98
SpeI A'CTAG_T
Cuts at: 0 742 3651
Size: 742 2909
SphI G_CATG'C
Cuts at: 0 2305 2793 3651
Size: 2305 488 858
Fragments arranged by size:
2305 858 488
SspI AAT'ATT
Cuts at: 0 1363 2109 3651
Size: 1363 746 1542
Fragments arranged by size:
1542 1363 746
StuI AGG'CCT
Cuts at: 0 3528 3651
Size: 3528 123
StyI C'CwwG_G
Cuts at: 0 926 3362 3651
Size: 926 2436 289
Fragments arranged by size:
2436 926 289
TatI w'GTAC_w
Cuts at: 0 2 2933 3057 3495 3651
Size: 2 2931 124 438 156
Fragments arranged by size:
2931 438 156 124 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3281 3313 3651
Size: 3281 32 338
Fragments arranged by size:
3281 338 32
Tth111I GACn'n_nGTC
Cuts at: 0 1969 3651
Size: 1969 1682
XbaI T'CTAG_A
Cuts at: 0 606 2191 3651
Size: 606 1585 1460
Fragments arranged by size:
1585 1460 606
XhoI C'TCGA_G
Cuts at: 0 1337 2477 3651
Size: 1337 1140 1174
Fragments arranged by size:
1337 1174 1140
XmnI GAAnn'nnTTC
Cuts at: 0 2254 3083 3651
Size: 2254 829 568
Fragments arranged by size:
2254 829 568
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApoI AseI AvaI BaeI BanI BanII
BbsI BcgI BciVI BclI BglII BlpI BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtsI ClaI DraI EaeI EarI
Eco57MI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NdeI NspI
PciI PflMI PfoI PspXI PsrI PvuII SexAI SmlI
SpeI SphI SspI StuI StyI TatI TstI Tth111I
XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AflII AhdI AloI ApaI ApaLI AscI
AsiSI AvrII BamHI BbeI BbvCI BfrBI BglI Bme1580I
BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaXI BseRI
BsgI BsiEI BsiWI BspEI BsrBI BsrDI BssHII BstZ17I
Bsu36I BtgI BtgZI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI FseI FspAI KasI KpnI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI SwaI TaqII XcmI XmaI
ZraI