Restriction map for
pF1KE1029
(Linear) (Six-base) MAPSORT of: KIEE1029.seq Check: 9447 from: 1 to: 3819
KIEE1029.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 747 3045 3819
Size: 747 2298 774
Fragments arranged by size:
2298 774 747
AccI GT'mk_AC
Cuts at: 0 428 2645 3615 3819
Size: 428 2217 970 204
Fragments arranged by size:
2217 970 428 204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 674 1360 1955 3819
Size: 382 292 686 595 1864
Fragments arranged by size:
1864 686 595 382 292
AfeI AGC'GCT
Cuts at: 0 3289 3819
Size: 3289 530
AgeI A'CCGG_T
Cuts at: 0 30 3819
Size: 30 3789
AhdI GACnn_n'nnGTC
Cuts at: 0 24 3819
Size: 24 3795
AleI CACnn'nnGTG
Cuts at: 0 853 2564 3819
Size: 853 1711 1255
Fragments arranged by size:
1711 1255 853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1037 3819
Size: 1037 2782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1290 1322 3819
Size: 1290 32 2497
Fragments arranged by size:
2497 1290 32
AlwNI CAG_nnn'CTG
Cuts at: 0 133 360 712 3819
Size: 133 227 352 3107
Fragments arranged by size:
3107 352 227 133
ApaI G_GGCC'C
Cuts at: 0 227 3819
Size: 227 3592
ApoI r'AATT_y
Cuts at: 0 158 457 1443 1528 1948 3261 3318 3513
Size: 158 299 986 85 420 1313 57 195
Cuts at: 3513 3819
Size: 306
Fragments arranged by size:
1313 986 420 306 299 195 158 85
57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1739 1772 3819
Size: 1739 33 2047
Fragments arranged by size:
2047 1739 33
BamHI G'GATC_C
Cuts at: 0 56 3395 3819
Size: 56 3339 424
Fragments arranged by size:
3339 424 56
BanI G'GyrC_C
Cuts at: 0 3726 3819
Size: 3726 93
BanII G_rGCy'C
Cuts at: 0 211 227 1666 3585 3819
Size: 211 16 1439 1919 234
Fragments arranged by size:
1919 1439 234 211 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1810 2351 3466 3819
Size: 1810 541 1115 353
Fragments arranged by size:
1810 1115 541 353
BbvCI CC'TCA_GC
Cuts at: 0 728 3819
Size: 728 3091
BclI T'GATC_A
Cuts at: 0 1206 3536 3819
Size: 1206 2330 283
Fragments arranged by size:
2330 1206 283
BfrBI ATG'CAT
Cuts at: 0 817 2448 3819
Size: 817 1631 1371
Fragments arranged by size:
1631 1371 817
BglI GCCn_nnn'nGGC
Cuts at: 0 1254 1268 2545 3819
Size: 1254 14 1277 1274
Fragments arranged by size:
1277 1274 1254 14
BglII A'GATC_T
Cuts at: 0 3044 3819
Size: 3044 775
BlpI GC'TnA_GC
Cuts at: 0 1493 3161 3819
Size: 1493 1668 658
Fragments arranged by size:
1668 1493 658
Bme1580I G_kGCm'C
Cuts at: 0 227 1978 3668 3731 3819
Size: 227 1751 1690 63 88
Fragments arranged by size:
1751 1690 227 88 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 690 3819
Size: 690 3129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 323 1500 2506 3819
Size: 323 1177 1006 1313
Fragments arranged by size:
1313 1177 1006 323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1034 1055 1292 1650 1990 3157 3514
Size: 1006 28 21 237 358 340 1167 357
Cuts at: 3514 3736 3819
Size: 222 83
Fragments arranged by size:
1167 1006 358 357 340 237 222 83
28 21
Bpu10I CC'TnA_GC
Cuts at: 0 728 3338 3819
Size: 728 2610 481
Fragments arranged by size:
2610 728 481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3108 3819
Size: 3108 711
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 1864 3679 3748 3819
Size: 130 1734 1815 69 71
Fragments arranged by size:
1815 1734 130 71 69
BsaAI yAC'GTr
Cuts at: 0 3303 3819
Size: 3303 516
BsaWI w'CCGG_w
Cuts at: 0 30 3097 3398 3819
Size: 30 3067 301 421
Fragments arranged by size:
3067 421 301 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 275 305 1205 1235 1290 1320 3130 3160
Size: 275 30 900 30 55 30 1810 30
Cuts at: 3160 3819
Size: 659
Fragments arranged by size:
1810 900 659 275 55 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 716 1712 2311 3271 3819
Size: 716 996 599 960 548
Fragments arranged by size:
996 960 716 599 548
BseYI C'CCAG_C
Cuts at: 0 44 178 506 683 705 716 1258 2790
Size: 44 134 328 177 22 11 542 1532
Cuts at: 2790 2877 3819
Size: 87 942
Fragments arranged by size:
1532 942 542 328 177 134 87 44
22 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 311 355 1448 2677 3073 3819
Size: 292 19 44 1093 1229 396 746
Fragments arranged by size:
1229 1093 746 396 292 44 19
BsiHKAI G_wGCw'C
Cuts at: 0 211 3819
Size: 211 3608
BsmI GAATG_Cn'
Cuts at: 0 979 2307 2408 3819
Size: 979 1328 101 1411
Fragments arranged by size:
1411 1328 979 101
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1096 2842 3819
Size: 1096 1746 977
Fragments arranged by size:
1746 1096 977
Bsp1286I G_dGCh'C
Cuts at: 0 211 227 1666 1978 3585 3668 3731 3819
Size: 211 16 1439 312 1607 83 63 88
Fragments arranged by size:
1607 1439 312 211 88 83 63 16
BspEI T'CCGG_A
Cuts at: 0 3398 3819
Size: 3398 421
BspHI T'CATG_A
Cuts at: 0 449 3819
Size: 449 3370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 747 3045 3819
Size: 747 2298 774
Fragments arranged by size:
2298 774 747
BsrDI GCAATG_nn'
Cuts at: 0 1622 2066 2975 3819
Size: 1622 444 909 844
Fragments arranged by size:
1622 909 844 444
BsrFI r'CCGG_y
Cuts at: 0 30 834 3819
Size: 30 804 2985
Fragments arranged by size:
2985 804 30
BsrGI T'GTAC_A
Cuts at: 0 1581 1708 3819
Size: 1581 127 2111
Fragments arranged by size:
2111 1581 127
BssSI C'ACGA_G
Cuts at: 0 1487 3819
Size: 1487 2332
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 3819
Size: 96 3723
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 3819
Size: 594 3225
BstYI r'GATC_y
Cuts at: 0 56 1277 3044 3395 3819
Size: 56 1221 1767 351 424
Fragments arranged by size:
1767 1221 424 351 56
BstZ17I GTA'TAC
Cuts at: 0 2646 3819
Size: 2646 1173
Bsu36I CC'TnA_GG
Cuts at: 0 422 2230 2710 3819
Size: 422 1808 480 1109
Fragments arranged by size:
1808 1109 480 422
BtgI C'CryG_G
Cuts at: 0 279 639 2987 3464 3819
Size: 279 360 2348 477 355
Fragments arranged by size:
2348 477 360 355 279
BtsI GCAGTG_nn'
Cuts at: 0 134 3819
Size: 134 3685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1356 1391 3819
Size: 1356 35 2428
Fragments arranged by size:
2428 1356 35
DraI TTT'AAA
Cuts at: 0 462 1098 1554 1742 2208 3819
Size: 462 636 456 188 466 1611
Fragments arranged by size:
1611 636 466 462 456 188
DraIII CAC_nnn'GTG
Cuts at: 0 96 2391 3819
Size: 96 2295 1428
Fragments arranged by size:
2295 1428 96
DrdI GACnn_nn'nnGTC
Cuts at: 0 144 2224 3819
Size: 144 2080 1595
Fragments arranged by size:
2080 1595 144
EaeI y'GGCC_r
Cuts at: 0 615 782 3819
Size: 615 167 3037
Fragments arranged by size:
3037 615 167
EarI CTCTTCn'nnn_
Cuts at: 0 1424 2389 2501 2513 2659 3248 3819
Size: 1424 965 112 12 146 589 571
Fragments arranged by size:
1424 965 589 571 146 112 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 821 3408 3819
Size: 821 2587 411
Fragments arranged by size:
2587 821 411
EcoICRI GAG'CTC
Cuts at: 0 209 3819
Size: 209 3610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 674 1006 1034 1055 1292 1360 1650
Size: 382 292 332 28 21 237 68 290
Cuts at: 1650 1955 1990 3157 3514 3736 3819
Size: 305 35 1167 357 222 83
Fragments arranged by size:
1167 382 357 332 305 292 290 237
222 83 68 35 28 21
EcoO109I rG'GnC_Cy
Cuts at: 0 2226 3720 3819
Size: 2226 1494 99
Fragments arranged by size:
2226 1494 99
EcoRI G'AATT_C
Cuts at: 0 158 3261 3318 3513 3819
Size: 158 3103 57 195 306
Fragments arranged by size:
3103 306 195 158 57
HaeII r_GCGC'y
Cuts at: 0 3291 3819
Size: 3291 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 364 396 1102 1134 1191 1205
Size: 10 32 322 32 706 32 57 14
Cuts at: 1205 1223 1237 2043 2075 3277 3309 3316 3348
Size: 18 14 806 32 1202 32 7 32
Cuts at: 3348 3819
Size: 471
Fragments arranged by size:
1202 806 706 471 322 57 32 32
32 32 32 32 18 14 14 10
7
HincII GTy'rAC
Cuts at: 0 2664 3143 3409 3819
Size: 2664 479 266 410
Fragments arranged by size:
2664 479 410 266
HindIII A'AGCT_T
Cuts at: 0 1915 3193 3819
Size: 1915 1278 626
Fragments arranged by size:
1915 1278 626
MfeI C'AATT_G
Cuts at: 0 2078 3819
Size: 2078 1741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 3819
Size: 34 3785
MscI TGG'CCA
Cuts at: 0 617 3819
Size: 617 3202
MslI CAynn'nnrTG
Cuts at: 0 853 1376 1627 2564 3599 3819
Size: 853 523 251 937 1035 220
Fragments arranged by size:
1035 937 853 523 251 220
MspA1I CmG'CkG
Cuts at: 0 268 325 2032 3021 3108 3153 3228 3819
Size: 268 57 1707 989 87 45 75 591
Fragments arranged by size:
1707 989 591 268 87 75 57 45
NcoI C'CATG_G
Cuts at: 0 639 2987 3464 3819
Size: 639 2348 477 355
Fragments arranged by size:
2348 639 477 355
NsiI A_TGCA'T
Cuts at: 0 819 2450 3819
Size: 819 1631 1369
Fragments arranged by size:
1631 1369 819
NspI r_CATG'y
Cuts at: 0 3664 3819
Size: 3664 155
PflMI CCAn_nnn'nTGG
Cuts at: 0 3695 3819
Size: 3695 124
PfoI T'CCnGG_A
Cuts at: 0 983 2885 3134 3819
Size: 983 1902 249 685
Fragments arranged by size:
1902 983 685 249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1290 1322 3819
Size: 1290 32 2497
Fragments arranged by size:
2497 1290 32
PpuMI rG'GwC_Cy
Cuts at: 0 2226 3819
Size: 2226 1593
PspOMI G'GGCC_C
Cuts at: 0 223 3819
Size: 223 3596
PstI C_TGCA'G
Cuts at: 0 407 3026 3819
Size: 407 2619 793
Fragments arranged by size:
2619 793 407
PvuII CAG'CTG
Cuts at: 0 325 2032 3021 3108 3228 3819
Size: 325 1707 989 87 120 591
Fragments arranged by size:
1707 989 591 325 120 87
SacI G_AGCT'C
Cuts at: 0 211 3819
Size: 211 3608
SapI GCTCTTCn'nnn_
Cuts at: 0 1424 3819
Size: 1424 2395
ScaI AGT'ACT
Cuts at: 0 166 3819
Size: 166 3653
SexAI A'CCwGG_T
Cuts at: 0 185 392 3819
Size: 185 207 3427
Fragments arranged by size:
3427 207 185
SfcI C'TryA_G
Cuts at: 0 230 403 1292 3022 3819
Size: 230 173 889 1730 797
Fragments arranged by size:
1730 889 797 230 173
SgrAI Cr'CCGG_yG
Cuts at: 0 30 3819
Size: 30 3789
SmlI C'TyrA_G
Cuts at: 0 3123 3819
Size: 3123 696
SpeI A'CTAG_T
Cuts at: 0 1506 3819
Size: 1506 2313
StuI AGG'CCT
Cuts at: 0 3634 3819
Size: 3634 185
StyI C'CwwG_G
Cuts at: 0 639 1025 1968 2222 2987 3213 3464 3653
Size: 639 386 943 254 765 226 251 189
Cuts at: 3653 3819
Size: 166
Fragments arranged by size:
943 765 639 386 254 251 226 189
166
SwaI ATTT'AAAT
Cuts at: 0 462 3819
Size: 462 3357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 275 652 3694 3819
Size: 192 83 377 3042 125
Fragments arranged by size:
3042 377 192 125 83
TatI w'GTAC_w
Cuts at: 0 164 1581 1708 3819
Size: 164 1417 127 2111
Fragments arranged by size:
2111 1417 164 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 3819
Size: 275 32 3512
Fragments arranged by size:
3512 275 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1953 3471 3819
Size: 1953 1518 348
Fragments arranged by size:
1953 1518 348
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApoI BaeI BamHI BanI BanII
BbsI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NcoI NsiI NspI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SapI ScaI SexAI
SfcI SgrAI SmlI SpeI StuI StyI SwaI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII ApaLI AscI AseI
AsiSI AvaI AvrII BbeI BcgI BciVI BmgBI BmtI
BsaBI BsaHI BsiEI BsiWI BsrBI BssHII BstBI BstEII
BtgZI ClaI EagI EcoNI EcoRV FalI FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SmaI SnaBI
SphI SrfI SspI Tth111I XbaI XhoI XmaI XmnI
ZraI