Restriction map for
pF1KE1030
(Linear) (Six-base) MAPSORT of: KIEE1030.seq Check: 621 from: 1 to: 3900
KIEE1030.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AccI GT'mk_AC
Cuts at: 0 914 1281 3900
Size: 914 367 2619
Fragments arranged by size:
2619 914 367
Acc65I G'GTAC_C
Cuts at: 0 555 3900
Size: 555 3345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 832 860 911 1778 1877 1967 2578
Size: 488 344 28 51 867 99 90 611
Cuts at: 2578 3224 3449 3829 3900
Size: 646 225 380 71
Fragments arranged by size:
867 646 611 488 380 344 225 99
90 71 51 28
AflII C'TTAA_G
Cuts at: 0 1909 2395 3900
Size: 1909 486 1505
Fragments arranged by size:
1909 1505 486
AflIII A'CryG_T
Cuts at: 0 1182 2727 3900
Size: 1182 1545 1173
Fragments arranged by size:
1545 1182 1173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2003 3900
Size: 2003 1897
AlwNI CAG_nnn'CTG
Cuts at: 0 1593 1968 3696 3900
Size: 1593 375 1728 204
Fragments arranged by size:
1728 1593 375 204
ApoI r'AATT_y
Cuts at: 0 1561 1751 1929 2075 2095 2313 3356 3900
Size: 1561 190 178 146 20 218 1043 544
Fragments arranged by size:
1561 1043 544 218 190 178 146 20
AvaI C'yCGr_G
Cuts at: 0 242 3900
Size: 242 3658
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1604 1637 3900
Size: 1604 33 2263
Fragments arranged by size:
2263 1604 33
BamHI G'GATC_C
Cuts at: 0 785 3900
Size: 785 3115
BanI G'GyrC_C
Cuts at: 0 555 3900
Size: 555 3345
BbsI GAAGACnn'nnnn_
Cuts at: 0 654 820 880 3773 3900
Size: 654 166 60 2893 127
Fragments arranged by size:
2893 654 166 127 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 495 3900
Size: 461 34 3405
Fragments arranged by size:
3405 461 34
BclI T'GATC_A
Cuts at: 0 282 2361 3900
Size: 282 2079 1539
Fragments arranged by size:
2079 1539 282
BfrBI ATG'CAT
Cuts at: 0 2207 3900
Size: 2207 1693
BlpI GC'TnA_GC
Cuts at: 0 1436 2351 3900
Size: 1436 915 1549
Fragments arranged by size:
1549 1436 915
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 3900
Size: 433 3467
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 257 3900
Size: 257 3643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2470 3718 3752 3900
Size: 2470 1248 34 148
Fragments arranged by size:
2470 1248 148 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 2812 3900
Size: 708 2104 1088
Fragments arranged by size:
2104 1088 708
BsaI GGTCTCn'nnnn_
Cuts at: 0 3034 3900
Size: 3034 866
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3784 3814 3900
Size: 3784 30 86
Fragments arranged by size:
3784 86 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2302 3265 3900
Size: 2302 963 635
Fragments arranged by size:
2302 963 635
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1972 1976 3900
Size: 1972 4 1924
Fragments arranged by size:
1972 1924 4
BsiHKAI G_wGCw'C
Cuts at: 0 3272 3884 3900
Size: 3272 612 16
Fragments arranged by size:
3272 612 16
BsmI GAATG_Cn'
Cuts at: 0 1437 2207 2301 3900
Size: 1437 770 94 1599
Fragments arranged by size:
1599 1437 770 94
Bsp1286I G_dGCh'C
Cuts at: 0 3272 3884 3900
Size: 3272 612 16
Fragments arranged by size:
3272 612 16
BspHI T'CATG_A
Cuts at: 0 83 2979 3900
Size: 83 2896 921
Fragments arranged by size:
2896 921 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 372 1698 3900
Size: 372 1326 2202
Fragments arranged by size:
2202 1326 372
BsrDI GCAATG_nn'
Cuts at: 0 577 1113 3900
Size: 577 536 2787
Fragments arranged by size:
2787 577 536
BsrGI T'GTAC_A
Cuts at: 0 2895 3900
Size: 2895 1005
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 3900
Size: 796 3104
BstYI r'GATC_y
Cuts at: 0 785 2694 3900
Size: 785 1909 1206
Fragments arranged by size:
1909 1206 785
BstZ17I GTA'TAC
Cuts at: 0 1282 3900
Size: 1282 2618
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 536 3900
Size: 536 3364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 713 748 3900
Size: 713 35 3152
Fragments arranged by size:
3152 713 35
DraI TTT'AAA
Cuts at: 0 252 852 956 2720 3900
Size: 252 600 104 1764 1180
Fragments arranged by size:
1764 1180 600 252 104
EaeI y'GGCC_r
Cuts at: 0 1568 3900
Size: 1568 2332
EarI CTCTTCn'nnn_
Cuts at: 0 73 371 1510 1685 1895 2051 2279 2435
Size: 73 298 1139 175 210 156 228 156
Cuts at: 2435 2645 2772 2821 3516 3900
Size: 210 127 49 695 384
Fragments arranged by size:
1139 695 384 298 228 210 210 175
156 156 127 73 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 832 860 911 1778 1877 1967 2470
Size: 488 344 28 51 867 99 90 503
Cuts at: 2470 2578 3224 3449 3718 3752 3829 3900
Size: 108 646 225 269 34 77 71
Fragments arranged by size:
867 646 503 488 344 269 225 108
99 90 77 71 51 34 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1965 3900
Size: 1965 1935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 877 1924 2266 3900
Size: 877 1047 342 1634
Fragments arranged by size:
1634 1047 877 342
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1148 1280 1312 3784 3816 3900
Size: 1116 32 132 32 2472 32 84
Fragments arranged by size:
2472 1116 132 84 32 32 32
HincII GTy'rAC
Cuts at: 0 1073 3900
Size: 1073 2827
HindIII A'AGCT_T
Cuts at: 0 136 629 1603 3900
Size: 136 493 974 2297
Fragments arranged by size:
2297 974 493 136
HpaI GTT'AAC
Cuts at: 0 1073 3900
Size: 1073 2827
KpnI G_GTAC'C
Cuts at: 0 559 3900
Size: 559 3341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1616 3101 3900
Size: 248 1368 1485 799
Fragments arranged by size:
1485 1368 799 248
MscI TGG'CCA
Cuts at: 0 1570 3900
Size: 1570 2330
MspA1I CmG'CkG
Cuts at: 0 475 1525 2883 3900
Size: 475 1050 1358 1017
Fragments arranged by size:
1358 1050 1017 475
NsiI A_TGCA'T
Cuts at: 0 2209 3900
Size: 2209 1691
NspI r_CATG'y
Cuts at: 0 1186 2731 3900
Size: 1186 1545 1169
Fragments arranged by size:
1545 1186 1169
PciI A'CATG_T
Cuts at: 0 1182 2727 3900
Size: 1182 1545 1173
Fragments arranged by size:
1545 1182 1173
PshAI GACnn'nnGTC
Cuts at: 0 725 3900
Size: 725 3175
PsiI TTA'TAA
Cuts at: 0 1117 3900
Size: 1117 2783
PstI C_TGCA'G
Cuts at: 0 1338 3900
Size: 1338 2562
PvuII CAG'CTG
Cuts at: 0 475 2883 3900
Size: 475 2408 1017
Fragments arranged by size:
2408 1017 475
SapI GCTCTTCn'nnn_
Cuts at: 0 371 2051 2435 3900
Size: 371 1680 384 1465
Fragments arranged by size:
1680 1465 384 371
SexAI A'CCwGG_T
Cuts at: 0 3887 3900
Size: 3887 13
SfcI C'TryA_G
Cuts at: 0 1334 1405 2149 2435 3370 3900
Size: 1334 71 744 286 935 530
Fragments arranged by size:
1334 935 744 530 286 71
SmlI C'TyrA_G
Cuts at: 0 242 723 1909 2395 2791 3900
Size: 242 481 1186 486 396 1109
Fragments arranged by size:
1186 1109 486 481 396 242
StuI AGG'CCT
Cuts at: 0 2764 3900
Size: 2764 1136
StyI C'CwwG_G
Cuts at: 0 1616 3900
Size: 1616 2284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 832 3900
Size: 832 3068
TatI w'GTAC_w
Cuts at: 0 1584 2895 3900
Size: 1584 1311 1005
Fragments arranged by size:
1584 1311 1005
Tth111I GACn'n_nGTC
Cuts at: 0 3798 3900
Size: 3798 102
XbaI T'CTAG_A
Cuts at: 0 3264 3900
Size: 3264 636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2416 3900
Size: 2416 1484
XhoI C'TCGA_G
Cuts at: 0 242 3900
Size: 242 3658
XmnI GAAnn'nnTTC
Cuts at: 0 1496 2444 3900
Size: 1496 948 1456
Fragments arranged by size:
1496 1456 948
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApoI
AvaI BaeI BamHI BanI BbsI BcgI BclI BfrBI
BlpI BmrI BplI BpmI BpuEI BsaI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstXI BstYI BstZ17I BtgZI CspCI DraI EaeI EarI
Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI KpnI
MmeI MscI MspA1I NsiI NspI PciI PshAI PsiI
PstI PvuII SapI SexAI SfcI SmlI StuI StyI
TaqII TatI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BanII BbeI
BbvCI BciVI BglI BglII Bme1580I BmgBI BmtI Bpu10I
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgI BtsI ClaI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
KasI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI XmaI ZraI