Restriction map for
pF1KE1031
(Linear) (Six-base) MAPSORT of: KIEE1031.seq Check: 4711 from: 1 to: 4179
KIEE1031.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2097 2867 4179
Size: 2097 770 1312
Fragments arranged by size:
2097 1312 770
AatII G_ACGT'C
Cuts at: 0 3616 4179
Size: 3616 563
Acc65I G'GTAC_C
Cuts at: 0 1454 4179
Size: 1454 2725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1066 1580 3323 3835 3851 4179
Size: 506 560 514 1743 512 16 328
Fragments arranged by size:
1743 560 514 512 506 328 16
AfeI AGC'GCT
Cuts at: 0 2900 4179
Size: 2900 1279
AflII C'TTAA_G
Cuts at: 0 1302 4179
Size: 1302 2877
AflIII A'CryG_T
Cuts at: 0 3753 4179
Size: 3753 426
AhdI GACnn_n'nnGTC
Cuts at: 0 1600 3084 4179
Size: 1600 1484 1095
Fragments arranged by size:
1600 1484 1095
AleI CACnn'nnGTG
Cuts at: 0 3897 4179
Size: 3897 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 3403 4015 4179
Size: 323 3080 612 164
Fragments arranged by size:
3080 612 323 164
AlwNI CAG_nnn'CTG
Cuts at: 0 829 1182 1675 2868 2910 3225 3531 4140
Size: 829 353 493 1193 42 315 306 609
Cuts at: 4140 4179
Size: 39
Fragments arranged by size:
1193 829 609 493 353 315 306 42
39
ApaI G_GGCC'C
Cuts at: 0 499 532 1263 2377 2841 3690 4179
Size: 499 33 731 1114 464 849 489
Fragments arranged by size:
1114 849 731 499 489 464 33
AvaI C'yCGr_G
Cuts at: 0 2625 2763 3197 3351 4179
Size: 2625 138 434 154 828
Fragments arranged by size:
2625 828 434 154 138
AvrII C'CTAG_G
Cuts at: 0 568 4179
Size: 568 3611
BanI G'GyrC_C
Cuts at: 0 541 1454 2112 3425 3899 4179
Size: 541 913 658 1313 474 280
Fragments arranged by size:
1313 913 658 541 474 280
BanII G_rGCy'C
Cuts at: 0 499 532 644 908 1263 1404 1433 1616
Size: 499 33 112 264 355 141 29 183
Cuts at: 1616 2081 2126 2300 2377 2841 3690 3774 3970
Size: 465 45 174 77 464 849 84 196
Cuts at: 3970 4097 4179
Size: 127 82
Fragments arranged by size:
849 499 465 464 355 264 196 183
174 141 127 112 84 82 77 45
33 29
BbeI G_GCGC'C
Cuts at: 0 3429 4179
Size: 3429 750
BbsI GAAGACnn'nnnn_
Cuts at: 0 1072 3839 4179
Size: 1072 2767 340
Fragments arranged by size:
2767 1072 340
BbvCI CC'TCA_GC
Cuts at: 0 283 1887 1961 2243 4179
Size: 283 1604 74 282 1936
Fragments arranged by size:
1936 1604 283 282 74
BclI T'GATC_A
Cuts at: 0 294 4179
Size: 294 3885
BglI GCCn_nnn'nGGC
Cuts at: 0 2755 3220 3466 4179
Size: 2755 465 246 713
Fragments arranged by size:
2755 713 465 246
BglII A'GATC_T
Cuts at: 0 787 4179
Size: 787 3392
BlpI GC'TnA_GC
Cuts at: 0 659 2008 2125 2658 2959 3007 4179
Size: 659 1349 117 533 301 48 1172
Fragments arranged by size:
1349 1172 659 533 301 117 48
Bme1580I G_kGCm'C
Cuts at: 0 371 499 521 532 1263 1759 2377 2384
Size: 371 128 22 11 731 496 618 7
Cuts at: 2384 2841 3382 3452 3690 3904 4179
Size: 457 541 70 238 214 275
Fragments arranged by size:
731 618 541 496 457 371 275 238
214 128 70 22 11 7
BmgBI CAC'GTC
Cuts at: 0 1107 4179
Size: 1107 3072
BmrI ACTGGGnnnn_n'
Cuts at: 0 365 2080 2371 2530 2626 3514 3786 4179
Size: 365 1715 291 159 96 888 272 393
Fragments arranged by size:
1715 888 393 365 291 272 159 96
BmtI G_CTAG'C
Cuts at: 0 1291 4179
Size: 1291 2888
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 3678 3800 4179
Size: 843 2835 122 379
Fragments arranged by size:
2835 843 379 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 946 1021 1439 1675 1771 2284 2445
Size: 133 813 75 418 236 96 513 161
Cuts at: 2445 2446 2755 2770 3046 3829 4117 4179
Size: 1 309 15 276 783 288 62
Fragments arranged by size:
813 783 513 418 309 288 276 236
161 133 96 75 62 15 1
Bpu10I CC'TnA_GC
Cuts at: 0 283 643 1887 1961 2243 3137 4179
Size: 283 360 1244 74 282 894 1042
Fragments arranged by size:
1244 1042 894 360 283 282 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 3551 4179
Size: 804 2747 628
Fragments arranged by size:
2747 804 628
BsaI GGTCTCn'nnnn_
Cuts at: 0 3 1811 4039 4168 4179
Size: 3 1808 2228 129 11
Fragments arranged by size:
2228 1808 129 11 3
BsaAI yAC'GTr
Cuts at: 0 1650 3756 4179
Size: 1650 2106 423
Fragments arranged by size:
2106 1650 423
BsaHI Gr'CG_yC
Cuts at: 0 3426 3613 4179
Size: 3426 187 566
Fragments arranged by size:
3426 566 187
BsaWI w'CCGG_w
Cuts at: 0 1934 3863 4179
Size: 1934 1929 316
Fragments arranged by size:
1934 1929 316
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 549 579 2464 2494 3810 3840 4179
Size: 549 30 1885 30 1316 30 339
Fragments arranged by size:
1885 1316 549 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 206 300 736 847 1162 1423 1441 2173
Size: 206 94 436 111 315 261 18 732
Cuts at: 2173 2500 2680 3535 4179
Size: 327 180 855 644
Fragments arranged by size:
855 732 644 436 327 315 261 206
180 111 94 18
BseYI C'CCAG_C
Cuts at: 0 81 198 235 755 766 939 1211 1486
Size: 81 117 37 520 11 173 272 275
Cuts at: 1486 1530 1891 2189 2602 3157 3551 4179
Size: 44 361 298 413 555 394 628
Fragments arranged by size:
628 555 520 413 394 361 298 275
272 173 117 81 44 37 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 95 109 964 1117 3211 4036 4179
Size: 28 67 14 855 153 2094 825 143
Fragments arranged by size:
2094 855 825 153 143 67 28 14
BsiHKAI G_wGCw'C
Cuts at: 0 1224 1395 1433 1706 2014 2081 2126 3774
Size: 1224 171 38 273 308 67 45 1648
Cuts at: 3774 3911 3970 4097 4157 4179
Size: 137 59 127 60 22
Fragments arranged by size:
1648 1224 308 273 171 137 127 67
60 59 45 38 22
BsmI GAATG_Cn'
Cuts at: 0 1531 1722 4179
Size: 1531 191 2457
Fragments arranged by size:
2457 1531 191
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2647 4179
Size: 2647 1532
Bsp1286I G_dGCh'C
Cuts at: 0 371 499 521 532 644 908 1224 1263
Size: 371 128 22 11 112 264 316 39
Cuts at: 1263 1395 1404 1433 1616 1706 1759 2014 2081
Size: 132 9 29 183 90 53 255 67
Cuts at: 2081 2126 2300 2377 2384 2841 3382 3452 3690
Size: 45 174 77 7 457 541 70 238
Cuts at: 3690 3774 3904 3911 3970 4097 4157 4179
Size: 84 130 7 59 127 60 22
Fragments arranged by size:
541 457 371 316 264 255 238 183
174 132 130 128 127 112 90 84
77 70 67 60 59 53 45 39
29 22 22 11 9 7 7
BspEI T'CCGG_A
Cuts at: 0 1934 3863 4179
Size: 1934 1929 316
Fragments arranged by size:
1934 1929 316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 1121 1683 2097 2867 4179
Size: 47 1074 562 414 770 1312
Fragments arranged by size:
1312 1074 770 562 414 47
BsrBI CCG'CTC
Cuts at: 0 147 1162 4179
Size: 147 1015 3017
Fragments arranged by size:
3017 1015 147
BsrDI GCAATG_nn'
Cuts at: 0 949 1373 3183 4179
Size: 949 424 1810 996
Fragments arranged by size:
1810 996 949 424
BsrFI r'CCGG_y
Cuts at: 0 137 1283 1571 2206 2963 4179
Size: 137 1146 288 635 757 1216
Fragments arranged by size:
1216 1146 757 635 288 137
BssSI C'ACGA_G
Cuts at: 0 2608 4179
Size: 2608 1571
BstAPI GCAn_nnn'nTGC
Cuts at: 0 73 747 2112 2910 3405 4050 4179
Size: 73 674 1365 798 495 645 129
Fragments arranged by size:
1365 798 674 645 495 129 73
BstEII G'GTnAC_C
Cuts at: 0 2062 4179
Size: 2062 2117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 592 1125 3210 3642 3682 4179
Size: 592 533 2085 432 40 497
Fragments arranged by size:
2085 592 533 497 432 40
BstYI r'GATC_y
Cuts at: 0 725 787 2717 4179
Size: 725 62 1930 1462
Fragments arranged by size:
1930 1462 725 62
Bsu36I CC'TnA_GG
Cuts at: 0 257 719 2542 4179
Size: 257 462 1823 1637
Fragments arranged by size:
1823 1637 462 257
BtgI C'CryG_G
Cuts at: 0 710 1140 1458 2116 2369 3374 4179
Size: 710 430 318 658 253 1005 805
Fragments arranged by size:
1005 805 710 658 430 318 253
BtsI GCAGTG_nn'
Cuts at: 0 92 246 305 1228 1302 2170 2500 2547
Size: 92 154 59 923 74 868 330 47
Cuts at: 2547 2681 2908 3406 4179
Size: 134 227 498 773
Fragments arranged by size:
923 868 773 498 330 227 154 134
92 74 59 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 329 364 4179
Size: 329 35 3815
Fragments arranged by size:
3815 329 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2289 4179
Size: 2289 1890
EaeI y'GGCC_r
Cuts at: 0 243 350 2052 2258 2736 4179
Size: 243 107 1702 206 478 1443
Fragments arranged by size:
1702 1443 478 243 206 107
EarI CTCTTCn'nnn_
Cuts at: 0 269 680 907 1336 1400 2702 4179
Size: 269 411 227 429 64 1302 1477
Fragments arranged by size:
1477 1302 429 411 269 227 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1699 3877 4179
Size: 1699 2178 302
Fragments arranged by size:
2178 1699 302
EcoICRI GAG'CTC
Cuts at: 0 1431 2079 2124 3772 3968 4095 4179
Size: 1431 648 45 1648 196 127 84
Fragments arranged by size:
1648 1431 648 196 127 84 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 506 946 1021 1066 1439 1580 1675
Size: 133 373 440 75 45 373 141 95
Cuts at: 1675 1771 2284 2445 2446 2755 2770 3046 3323
Size: 96 513 161 1 309 15 276 277
Cuts at: 3323 3829 3835 3851 4117 4179
Size: 506 6 16 266 62
Fragments arranged by size:
513 506 440 373 373 309 277 276
266 161 141 133 96 95 75 62
45 16 15 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 2482 4179
Size: 256 2226 1697
Fragments arranged by size:
2226 1697 256
EcoO109I rG'GnC_Cy
Cuts at: 0 267 495 616 850 1259 1672 1746 1822
Size: 267 228 121 234 409 413 74 76
Cuts at: 1822 2019 2838 3150 3393 3686 3687 3957 4179
Size: 197 819 312 243 293 1 270 222
Fragments arranged by size:
819 413 409 312 293 270 267 243
234 228 222 197 121 76 74 1
HaeII r_GCGC'y
Cuts at: 0 2478 2902 3429 4179
Size: 2478 424 527 750
Fragments arranged by size:
2478 750 527 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 279 311 549 581 1053 1085
Size: 115 32 132 32 238 32 472 32
Cuts at: 1085 1461 1493 1586 1618 2464 2496 3070 3102
Size: 376 32 93 32 846 32 574 32
Cuts at: 3102 3334 3366 4179
Size: 232 32 813
Fragments arranged by size:
846 813 574 472 376 238 232 132
115 93 32 32 32 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 4060 4179
Size: 4060 119
KasI G'GCGC_C
Cuts at: 0 3425 4179
Size: 3425 754
KpnI G_GTAC'C
Cuts at: 0 1458 4179
Size: 1458 2721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 4179
Size: 155 4024
MscI TGG'CCA
Cuts at: 0 245 2054 2738 4179
Size: 245 1809 684 1441
Fragments arranged by size:
1809 1441 684 245
MslI CAynn'nnrTG
Cuts at: 0 161 1139 3385 3481 3897 4179
Size: 161 978 2246 96 416 282
Fragments arranged by size:
2246 978 416 282 161 96
MspA1I CmG'CkG
Cuts at: 0 70 93 744 1891 2118 2160 2571 2742
Size: 70 23 651 1147 227 42 411 171
Cuts at: 2742 3084 3285 4179
Size: 342 201 894
Fragments arranged by size:
1147 894 651 411 342 227 201 171
70 42 23
NaeI GCC'GGC
Cuts at: 0 1573 2208 2965 4179
Size: 1573 635 757 1214
Fragments arranged by size:
1573 1214 757 635
NarI GG'CG_CC
Cuts at: 0 3426 4179
Size: 3426 753
NcoI C'CATG_G
Cuts at: 0 710 1140 1458 3374 4179
Size: 710 430 318 1916 805
Fragments arranged by size:
1916 805 710 430 318
NgoMIV G'CCGG_C
Cuts at: 0 1571 2206 2963 4179
Size: 1571 635 757 1216
Fragments arranged by size:
1571 1216 757 635
NheI G'CTAG_C
Cuts at: 0 1287 4179
Size: 1287 2892
NspI r_CATG'y
Cuts at: 0 1237 4179
Size: 1237 2942
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 1802 1977 2469 2793 3299 4179
Size: 505 1297 175 492 324 506 880
Fragments arranged by size:
1297 880 506 505 492 324 175
PfoI T'CCnGG_A
Cuts at: 0 149 1416 1748 2462 3035 3509 3641 4179
Size: 149 1267 332 714 573 474 132 538
Fragments arranged by size:
1267 714 573 538 474 332 149 132
PmlI CAC'GTG
Cuts at: 0 3756 4179
Size: 3756 423
PpuMI rG'GwC_Cy
Cuts at: 0 267 616 850 1672 1746 3150 3393 3957
Size: 267 349 234 822 74 1404 243 564
Cuts at: 3957 4179
Size: 222
Fragments arranged by size:
1404 822 564 349 267 243 234 222
74
PspOMI G'GGCC_C
Cuts at: 0 495 528 1259 2373 2837 3686 4179
Size: 495 33 731 1114 464 849 493
Fragments arranged by size:
1114 849 731 495 493 464 33
PstI C_TGCA'G
Cuts at: 0 707 734 2864 2906 3401 3710 4007 4179
Size: 707 27 2130 42 495 309 297 172
Fragments arranged by size:
2130 707 495 309 297 172 42 27
PvuII CAG'CTG
Cuts at: 0 70 93 1891 2742 3084 4179
Size: 70 23 1798 851 342 1095
Fragments arranged by size:
1798 1095 851 342 70 23
SacI G_AGCT'C
Cuts at: 0 1433 2081 2126 3774 3970 4097 4179
Size: 1433 648 45 1648 196 127 82
Fragments arranged by size:
1648 1433 648 196 127 82 45
SacII CC_GC'GG
Cuts at: 0 2119 4179
Size: 2119 2060
SanDI GG'GwC_CC
Cuts at: 0 616 4179
Size: 616 3563
SapI GCTCTTCn'nnn_
Cuts at: 0 1400 4179
Size: 1400 2779
SbfI CC_TGCA'GG
Cuts at: 0 734 4179
Size: 734 3445
SfcI C'TryA_G
Cuts at: 0 703 730 1174 1775 2860 2902 3241 3397
Size: 703 27 444 601 1085 42 339 156
Cuts at: 3397 3706 4003 4179
Size: 309 297 176
Fragments arranged by size:
1085 703 601 444 339 309 297 176
156 42 27
SfoI GGC'GCC
Cuts at: 0 3427 4179
Size: 3427 752
SmaI CCC'GGG
Cuts at: 0 2765 4179
Size: 2765 1414
SmlI C'TyrA_G
Cuts at: 0 783 1302 3566 4179
Size: 783 519 2264 613
Fragments arranged by size:
2264 783 613 519
SspI AAT'ATT
Cuts at: 0 1902 4179
Size: 1902 2277
StuI AGG'CCT
Cuts at: 0 2422 3142 4179
Size: 2422 720 1037
Fragments arranged by size:
2422 1037 720
StyI C'CwwG_G
Cuts at: 0 491 568 710 869 1140 1349 1458 2948
Size: 491 77 142 159 271 209 109 1490
Cuts at: 2948 3374 4179
Size: 426 805
Fragments arranged by size:
1490 805 491 426 271 209 159 142
109 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 576 1135 2146 2150 2203 2935 3007
Size: 27 549 559 1011 4 53 732 72
Cuts at: 3007 3393 4179
Size: 386 786
Fragments arranged by size:
1011 786 732 559 549 386 72 53
27 4
TatI w'GTAC_w
Cuts at: 0 3522 4179
Size: 3522 657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1664 1696 3810 3842 4179
Size: 1664 32 2114 32 337
Fragments arranged by size:
2114 1664 337 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 991 4179
Size: 991 3188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1746 3372 4179
Size: 1746 1626 807
Fragments arranged by size:
1746 1626 807
XmaI C'CCGG_G
Cuts at: 0 2763 4179
Size: 2763 1416
XmnI GAAnn'nnTTC
Cuts at: 0 2779 4179
Size: 2779 1400
ZraI GAC'GTC
Cuts at: 0 3614 4179
Size: 3614 565
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI SfcI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AgeI AloI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BcgI BciVI BfrBI BsaBI BsiEI
BsiWI BspHI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
DraI DraIII EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI MfeI MluI NdeI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XbaI
XhoI