(Linear) (Six-base) MAPSORT of: KIEE1037.seq  Check: 8767  from: 1  to: 1161



KIEE1037.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     961    1161 
   Size:        292     669     200

  Fragments arranged by size: 

                 669     292     200
 
AflII C'TTAA_G

Cuts at:      0    1134    1161 
   Size:       1134      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1066    1161 
   Size:       1066      95
 
ApoI r'AATT_y

Cuts at:      0     253    1161 
   Size:        253     908
 
BanII G_rGCy'C

Cuts at:      0      42     352     359    1161 
   Size:         42     310       7     802

  Fragments arranged by size: 

                 802     310      42       7
 
BfrBI ATG'CAT

Cuts at:      0     409    1161 
   Size:        409     752
 
BlpI GC'TnA_GC

Cuts at:      0     595    1161 
   Size:        595     566
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     354     596    1113    1161 
   Size:        354     242     517      48

  Fragments arranged by size: 

                 517     354     242      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     325    1161 
   Size:        325     836
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343    1161 
   Size:        343     818
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415    1161 
   Size:        415     746
 
BsaBI GATnn'nnATC

Cuts at:      0      52    1161 
   Size:         52    1109
 
BsaWI w'CCGG_w

Cuts at:      0     457    1161 
   Size:        457     704
 
BseYI C'CCAG_C

Cuts at:      0      87    1161 
   Size:         87    1074
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     359    1161 
   Size:        121     238     802

  Fragments arranged by size: 

                 802     238     121
 
BsmI GAATG_Cn'

Cuts at:      0     340     742    1161 
   Size:        340     402     419

  Fragments arranged by size: 

                 419     402     340
 
Bsp1286I G_dGCh'C

Cuts at:      0      42     121     352     359    1161 
   Size:         42      79     231       7     802

  Fragments arranged by size: 

                 802     231      79      42       7
 
BspHI T'CATG_A

Cuts at:      0     959    1161 
   Size:        959     202
 
BsrGI T'GTAC_A

Cuts at:      0     221     810    1161 
   Size:        221     589     351

  Fragments arranged by size: 

                 589     351     221
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      95    1161 
   Size:         95    1066
 
BstYI r'GATC_y

Cuts at:      0      53    1161 
   Size:         53    1108
 
Bsu36I CC'TnA_GG

Cuts at:      0     737    1161 
   Size:        737     424
 
BtsI GCAGTG_nn'

Cuts at:      0     651    1161 
   Size:        651     510
 
ClaI AT'CG_AT

Cuts at:      0     560    1161 
   Size:        560     601
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     554     589    1161 
   Size:        554      35     572

  Fragments arranged by size: 

                 572     554      35
 
EaeI y'GGCC_r

Cuts at:      0    1041    1161 
   Size:       1041     120
 
EarI CTCTTCn'nnn_

Cuts at:      0      68    1161 
   Size:         68    1093
 
EcoICRI GAG'CTC

Cuts at:      0     357    1161 
   Size:        357     804
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     343     961    1161 
   Size:        292      51     618     200

  Fragments arranged by size: 

                 618     292     200      51
 
EcoRI G'AATT_C

Cuts at:      0     253    1161 
   Size:        253     908
 
EcoRV GAT'ATC

Cuts at:      0    1073    1161 
   Size:       1073      88
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     615    1002    1161 
   Size:        615     387     159

  Fragments arranged by size: 

                 615     387     159
 
HaeII r_GCGC'y

Cuts at:      0    1032    1161 
   Size:       1032     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93    1161 
   Size:         61      32    1068

  Fragments arranged by size: 

                1068      61      32
 
HincII GTy'rAC

Cuts at:      0     954    1161 
   Size:        954     207
 
HindIII A'AGCT_T

Cuts at:      0     597    1161 
   Size:        597     564
 
MslI CAynn'nnrTG

Cuts at:      0     248     414     787    1161 
   Size:        248     166     373     374

  Fragments arranged by size: 

                 374     373     248     166
 
MspA1I CmG'CkG

Cuts at:      0      91    1161 
   Size:         91    1070
 
NsiI A_TGCA'T

Cuts at:      0     411    1161 
   Size:        411     750
 
NspI r_CATG'y

Cuts at:      0     409    1161 
   Size:        409     752
 
PshAI GACnn'nnGTC

Cuts at:      0     110    1161 
   Size:        110    1051
 
PvuII CAG'CTG

Cuts at:      0      91    1161 
   Size:         91    1070
 
SacI G_AGCT'C

Cuts at:      0     359    1161 
   Size:        359     802
 
SmlI C'TyrA_G

Cuts at:      0     430    1134    1161 
   Size:        430     704      27

  Fragments arranged by size: 

                 704     430      27
 
SspI AAT'ATT

Cuts at:      0     896    1161 
   Size:        896     265
 
TatI w'GTAC_w

Cuts at:      0     221     810    1161 
   Size:        221     589     351

  Fragments arranged by size: 

                 589     351     221
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     597     629    1161 
   Size:        597      32     532

  Fragments arranged by size: 

                 597     532      32
 
XbaI T'CTAG_A

Cuts at:      0    1083    1161 
   Size:       1083      78
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     631    1161 
   Size:        631     530
 
XmnI GAAnn'nnTTC

Cuts at:      0     917    1161 
   Size:        917     244
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI    BanII    BfrBI     BlpI     BmrI
     BplI     BpmI    BpuEI    BsaBI    BsaWI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrGI    BstXI    BstYI   Bsu36I     BtsI     ClaI
    CspCI     EaeI     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     MslI   MspA1I     NsiI     NspI
    PshAI    PvuII     SacI     SmlI     SspI     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI
     BmtI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII  Tth111I     XhoI
     XmaI     ZraI