(Linear) (Six-base) MAPSORT of: KIEE1039.seq  Check: 299  from: 1  to: 1485



KIEE1039.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AflIII A'CryG_T

Cuts at:      0     514     848    1485 
   Size:        514     334     637

  Fragments arranged by size: 

                 637     514     334
 
AlwNI CAG_nnn'CTG

Cuts at:      0     892    1056    1485 
   Size:        892     164     429

  Fragments arranged by size: 

                 892     429     164
 
ApoI r'AATT_y

Cuts at:      0      77     154     255     466     838    1045    1485 
   Size:         77      77     101     211     372     207     440

  Fragments arranged by size: 

                 440     372     211     207     101      77      77
 
AseI AT'TA_AT

Cuts at:      0     647    1485 
   Size:        647     838
 
BanI G'GyrC_C

Cuts at:      0     830    1485 
   Size:        830     655
 
BanII G_rGCy'C

Cuts at:      0     493    1485 
   Size:        493     992
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      55     931    1485 
   Size:         55     876     554

  Fragments arranged by size: 

                 876     554      55
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1072    1485 
   Size:       1072     413
 
BclI T'GATC_A

Cuts at:      0     410    1485 
   Size:        410    1075
 
BfrBI ATG'CAT

Cuts at:      0    1163    1485 
   Size:       1163     322
 
BglII A'GATC_T

Cuts at:      0    1344    1485 
   Size:       1344     141
 
BlpI GC'TnA_GC

Cuts at:      0     554    1485 
   Size:        554     931
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     588    1485 
   Size:        588     897
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     521    1485 
   Size:        348     173     964

  Fragments arranged by size: 

                 964     348     173
 
Bpu10I CC'TnA_GC

Cuts at:      0     563    1485 
   Size:        563     922
 
Bsp1286I G_dGCh'C

Cuts at:      0     493    1485 
   Size:        493     992
 
BspHI T'CATG_A

Cuts at:      0      44    1485 
   Size:         44    1441
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     380    1485 
   Size:        380    1105
 
BsrDI GCAATG_nn'

Cuts at:      0      98     110    1485 
   Size:         98      12    1375

  Fragments arranged by size: 

                1375      98      12
 
BsrGI T'GTAC_A

Cuts at:      0     843     851    1485 
   Size:        843       8     634

  Fragments arranged by size: 

                 843     634       8
 
BstYI r'GATC_y

Cuts at:      0     210     540    1344    1485 
   Size:        210     330     804     141

  Fragments arranged by size: 

                 804     330     210     141
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     881    1485 
   Size:        881     604
 
DraI TTT'AAA

Cuts at:      0     377     767    1485 
   Size:        377     390     718

  Fragments arranged by size: 

                 718     390     377
 
EaeI y'GGCC_r

Cuts at:      0      11    1485 
   Size:         11    1474
 
EarI CTCTTCn'nnn_

Cuts at:      0     327     521     674    1247    1352    1485 
   Size:        327     194     153     573     105     133

  Fragments arranged by size: 

                 573     327     194     153     133     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     348     521    1485 
   Size:        348     173     964

  Fragments arranged by size: 

                 964     348     173
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1306    1485 
   Size:       1306     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      99     131    1485 
   Size:         99      32    1354

  Fragments arranged by size: 

                1354      99      32
 
HincII GTy'rAC

Cuts at:      0     627    1485 
   Size:        627     858
 
HindIII A'AGCT_T

Cuts at:      0     249    1485 
   Size:        249    1236
 
HpaI GTT'AAC

Cuts at:      0     627    1485 
   Size:        627     858
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1485 
   Size:        205    1280
 
MslI CAynn'nnrTG

Cuts at:      0     103    1040    1485 
   Size:        103     937     445

  Fragments arranged by size: 

                 937     445     103
 
MspA1I CmG'CkG

Cuts at:      0     665     726     889    1441    1485 
   Size:        665      61     163     552      44

  Fragments arranged by size: 

                 665     552     163      61      44
 
NsiI A_TGCA'T

Cuts at:      0    1165    1485 
   Size:       1165     320
 
NspI r_CATG'y

Cuts at:      0     852    1485 
   Size:        852     633
 
PciI A'CATG_T

Cuts at:      0     848    1485 
   Size:        848     637
 
PstI C_TGCA'G

Cuts at:      0     281    1485 
   Size:        281    1204
 
PvuII CAG'CTG

Cuts at:      0     665     889    1485 
   Size:        665     224     596

  Fragments arranged by size: 

                 665     596     224
 
SfcI C'TryA_G

Cuts at:      0     277     356     521    1139    1485 
   Size:        277      79     165     618     346

  Fragments arranged by size: 

                 618     346     277     165      79
 
TatI w'GTAC_w

Cuts at:      0     843     851    1485 
   Size:        843       8     634

  Fragments arranged by size: 

                 843     634       8
 
XmnI GAAnn'nnTTC

Cuts at:      0     899    1485 
   Size:        899     586
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     AseI     BanI    BanII     BbsI    BciVI
     BclI    BfrBI    BglII     BlpI     BplI     BpmI   Bpu10I Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BstYI    BtgZI     DraI     EaeI
     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII     HpaI     MmeI
     MslI   MspA1I     NsiI     NspI     PciI     PstI    PvuII     SfcI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
     BglI Bme1580I    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI