Restriction map for
pF1KE1043
(Linear) (Six-base) MAPSORT of: KIEE1043.seq Check: 6648 from: 1 to: 1410
KIEE1043.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 106 1029 1410
Size: 106 923 381
Fragments arranged by size:
923 381 106
AccI GT'mk_AC
Cuts at: 0 494 1410
Size: 494 916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 736 981 1410
Size: 426 310 245 429
Fragments arranged by size:
429 426 310 245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1061 1410
Size: 1061 349
AlwNI CAG_nnn'CTG
Cuts at: 0 873 1410
Size: 873 537
ApoI r'AATT_y
Cuts at: 0 1245 1410
Size: 1245 165
AvaI C'yCGr_G
Cuts at: 0 692 1410
Size: 692 718
BanI G'GyrC_C
Cuts at: 0 410 514 779 1114 1410
Size: 410 104 265 335 296
Fragments arranged by size:
410 335 296 265 104
BanII G_rGCy'C
Cuts at: 0 1276 1410
Size: 1276 134
BbeI G_GCGC'C
Cuts at: 0 414 1410
Size: 414 996
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 1359 1410
Size: 144 1215 51
Fragments arranged by size:
1215 144 51
BbvCI CC'TCA_GC
Cuts at: 0 1405 1410
Size: 1405 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 865 899 1410
Size: 865 34 511
Fragments arranged by size:
865 511 34
BglII A'GATC_T
Cuts at: 0 194 1410
Size: 194 1216
BlpI GC'TnA_GC
Cuts at: 0 361 1410
Size: 361 1049
Bme1580I G_kGCm'C
Cuts at: 0 55 163 481 519 565 643 685 784
Size: 55 108 318 38 46 78 42 99
Cuts at: 784 849 1081 1410
Size: 65 232 329
Fragments arranged by size:
329 318 232 108 99 78 65 55
46 42 38
BmgBI CAC'GTC
Cuts at: 0 1209 1410
Size: 1209 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 294 916 1410
Size: 46 248 622 494
Fragments arranged by size:
622 494 248 46
Bpu10I CC'TnA_GC
Cuts at: 0 785 1405 1410
Size: 785 620 5
Fragments arranged by size:
785 620 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1009 1153 1410
Size: 400 609 144 257
Fragments arranged by size:
609 400 257 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 1210 1410
Size: 1210 200
BsaAI yAC'GTr
Cuts at: 0 1265 1410
Size: 1265 145
BsaHI Gr'CG_yC
Cuts at: 0 411 1410
Size: 411 999
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 826 913 1410
Size: 274 552 87 497
Fragments arranged by size:
552 497 274 87
BseYI C'CCAG_C
Cuts at: 0 542 1002 1410
Size: 542 460 408
Fragments arranged by size:
542 460 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 365 567 576 1410
Size: 106 259 202 9 834
Fragments arranged by size:
834 259 202 106 9
BsiHKAI G_wGCw'C
Cuts at: 0 746 929 1410
Size: 746 183 481
Fragments arranged by size:
746 481 183
BsmI GAATG_Cn'
Cuts at: 0 1048 1410
Size: 1048 362
Bsp1286I G_dGCh'C
Cuts at: 0 55 163 481 519 565 643 685 746
Size: 55 108 318 38 46 78 42 61
Cuts at: 746 784 849 929 1081 1276 1410
Size: 38 65 80 152 195 134
Fragments arranged by size:
318 195 152 134 108 80 78 65
61 55 46 42 38 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 106 130 322 387 710 1029 1410
Size: 78 28 24 192 65 323 319 381
Fragments arranged by size:
381 323 319 192 78 65 28 24
BsrDI GCAATG_nn'
Cuts at: 0 800 1068 1410
Size: 800 268 342
Fragments arranged by size:
800 342 268
BsrFI r'CCGG_y
Cuts at: 0 857 1410
Size: 857 553
BsrGI T'GTAC_A
Cuts at: 0 1094 1410
Size: 1094 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 590 873 1410
Size: 590 283 537
Fragments arranged by size:
590 537 283
BstBI TT'CG_AA
Cuts at: 0 1335 1410
Size: 1335 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1265 1410
Size: 1265 145
BstYI r'GATC_y
Cuts at: 0 194 1400 1410
Size: 194 1206 10
Fragments arranged by size:
1206 194 10
Bsu36I CC'TnA_GG
Cuts at: 0 164 1410
Size: 164 1246
BtsI GCAGTG_nn'
Cuts at: 0 921 1074 1410
Size: 921 153 336
Fragments arranged by size:
921 336 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 654 689 1050 1085 1410
Size: 654 35 361 35 325
Fragments arranged by size:
654 361 325 35 35
EaeI y'GGCC_r
Cuts at: 0 102 833 1410
Size: 102 731 577
Fragments arranged by size:
731 577 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 294 426 736 916 981 1410
Size: 46 248 132 310 180 65 429
Fragments arranged by size:
429 310 248 180 132 65 46
EcoO109I rG'GnC_Cy
Cuts at: 0 699 1410
Size: 699 711
HaeII r_GCGC'y
Cuts at: 0 414 1410
Size: 414 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 889 921 1410
Size: 889 32 489
Fragments arranged by size:
889 489 32
KasI G'GCGC_C
Cuts at: 0 410 1410
Size: 410 1000
MscI TGG'CCA
Cuts at: 0 835 1410
Size: 835 575
MslI CAynn'nnrTG
Cuts at: 0 1022 1102 1410
Size: 1022 80 308
Fragments arranged by size:
1022 308 80
MspA1I CmG'CkG
Cuts at: 0 245 272 725 1410
Size: 245 27 453 685
Fragments arranged by size:
685 453 245 27
NaeI GCC'GGC
Cuts at: 0 859 1410
Size: 859 551
NarI GG'CG_CC
Cuts at: 0 411 1410
Size: 411 999
NgoMIV G'CCGG_C
Cuts at: 0 857 1410
Size: 857 553
NspI r_CATG'y
Cuts at: 0 550 808 1410
Size: 550 258 602
Fragments arranged by size:
602 550 258
PasI CC'CwG_GG
Cuts at: 0 1186 1410
Size: 1186 224
PfoI T'CCnGG_A
Cuts at: 0 23 1410
Size: 23 1387
PmlI CAC'GTG
Cuts at: 0 1265 1410
Size: 1265 145
PpuMI rG'GwC_Cy
Cuts at: 0 699 1410
Size: 699 711
PstI C_TGCA'G
Cuts at: 0 131 319 878 1040 1410
Size: 131 188 559 162 370
Fragments arranged by size:
559 370 188 162 131
PvuII CAG'CTG
Cuts at: 0 725 1410
Size: 725 685
SbfI CC_TGCA'GG
Cuts at: 0 131 1410
Size: 131 1279
SfcI C'TryA_G
Cuts at: 0 127 295 315 874 961 1036 1410
Size: 127 168 20 559 87 75 374
Fragments arranged by size:
559 374 168 127 87 75 20
SfoI GGC'GCC
Cuts at: 0 412 1410
Size: 412 998
SmlI C'TyrA_G
Cuts at: 0 415 988 1132 1410
Size: 415 573 144 278
Fragments arranged by size:
573 415 278 144
SphI G_CATG'C
Cuts at: 0 550 1410
Size: 550 860
StuI AGG'CCT
Cuts at: 0 454 1410
Size: 454 956
StyI C'CwwG_G
Cuts at: 0 643 971 1410
Size: 643 328 439
Fragments arranged by size:
643 439 328
TatI w'GTAC_w
Cuts at: 0 219 1094 1410
Size: 219 875 316
Fragments arranged by size:
875 316 219
XmnI GAAnn'nnTTC
Cuts at: 0 1246 1410
Size: 1246 164
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtsI CspCI
EaeI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PfoI PmlI
PpuMI PstI PvuII SbfI SfcI SfoI SmlI SphI
StuI StyI TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglI BmrI BmtI
BplI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BssHII BssSI BstEII BstZ17I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI