(Linear) (Six-base) MAPSORT of: KIEE1046.seq  Check: 6276  from: 1  to: 435



KIEE1046.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AvaI C'yCGr_G

Cuts at:      0     425     435 
   Size:        425      10
 
BanI G'GyrC_C

Cuts at:      0      62     435 
   Size:         62     373
 
BanII G_rGCy'C

Cuts at:      0     374     435 
   Size:        374      61
 
BglI GCCn_nnn'nGGC

Cuts at:      0     350     435 
   Size:        350      85
 
Bme1580I G_kGCm'C

Cuts at:      0     346     435 
   Size:        346      89
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     242     435 
   Size:        242     193
 
BsaHI Gr'CG_yC

Cuts at:      0     226     435 
   Size:        226     209
 
BsaWI w'CCGG_w

Cuts at:      0      29     435 
   Size:         29     406
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      85     115     435 
   Size:         85      30     320

  Fragments arranged by size: 

                 320      85      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     112     435 
   Size:        109       3     323

  Fragments arranged by size: 

                 323     109       3
 
BseYI C'CCAG_C

Cuts at:      0     348     435 
   Size:        348      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     274     435 
   Size:         31     243     161

  Fragments arranged by size: 

                 243     161      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     374     435 
   Size:        374      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     217     435 
   Size:        217     218
 
Bsp1286I G_dGCh'C

Cuts at:      0     346     374     435 
   Size:        346      28      61

  Fragments arranged by size: 

                 346      61      28
 
BspHI T'CATG_A

Cuts at:      0     248     435 
   Size:        248     187
 
BsrBI CCG'CTC

Cuts at:      0     137     435 
   Size:        137     298
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     435 
   Size:        130     305
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     411     435 
   Size:        411      24
 
EcoICRI GAG'CTC

Cuts at:      0     372     435 
   Size:        372      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     271     435 
   Size:        271     164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     195     435 
   Size:        195     240
 
MspA1I CmG'CkG

Cuts at:      0     348     435 
   Size:        348      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     271     435 
   Size:        271     164
 
SacI G_AGCT'C

Cuts at:      0     374     435 
   Size:        374      61
 
SanDI GG'GwC_CC

Cuts at:      0     271     435 
   Size:        271     164
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130     435 
   Size:        130     305
 
SfcI C'TryA_G

Cuts at:      0      51     300     435 
   Size:         51     249     135

  Fragments arranged by size: 

                 249     135      51
 
SmaI CCC'GGG

Cuts at:      0     427     435 
   Size:        427       8
 
SrfI GCCC'GGGC

Cuts at:      0     427     435 
   Size:        427       8
 
StyI C'CwwG_G

Cuts at:      0      89     435 
   Size:         89     346
 
XmaI C'CCGG_G

Cuts at:      0     425     435 
   Size:        425      10
 

 Enzymes that do cut:

     AvaI     BanI    BanII     BglI Bme1580I     BmrI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BsrBI     EarI     EciI  EcoICRI EcoO109I     FalI   MspA1I    PpuMI
     SacI    SanDI     SapI     SfcI     SmaI     SrfI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI