Restriction map for
pF1KE1047
(Linear) (Six-base) MAPSORT of: KIEE1047.seq Check: 7786 from: 1 to: 1038
KIEE1047.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 974 1038
Size: 974 64
AccI GT'mk_AC
Cuts at: 0 413 1038
Size: 413 625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1038
Size: 493 545
AflIII A'CryG_T
Cuts at: 0 834 1038
Size: 834 204
AlwNI CAG_nnn'CTG
Cuts at: 0 243 711 876 1038
Size: 243 468 165 162
Fragments arranged by size:
468 243 165 162
ApoI r'AATT_y
Cuts at: 0 664 1038
Size: 664 374
AvaI C'yCGr_G
Cuts at: 0 130 608 1038
Size: 130 478 430
Fragments arranged by size:
478 430 130
BanII G_rGCy'C
Cuts at: 0 754 1038
Size: 754 284
BbsI GAAGACnn'nnnn_
Cuts at: 0 343 434 1038
Size: 343 91 604
Fragments arranged by size:
604 343 91
BglI GCCn_nnn'nGGC
Cuts at: 0 691 1038
Size: 691 347
BglII A'GATC_T
Cuts at: 0 533 1038
Size: 533 505
BlpI GC'TnA_GC
Cuts at: 0 733 1038
Size: 733 305
BmrI ACTGGGnnnn_n'
Cuts at: 0 1023 1038
Size: 1023 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 418 425 1038
Size: 108 310 7 613
Fragments arranged by size:
613 310 108 7
Bpu10I CC'TnA_GC
Cuts at: 0 244 1038
Size: 244 794
BsaAI yAC'GTr
Cuts at: 0 549 1038
Size: 549 489
BsaBI GATnn'nnATC
Cuts at: 0 8 538 1038
Size: 8 530 500
Fragments arranged by size:
530 500 8
BsaHI Gr'CG_yC
Cuts at: 0 431 1038
Size: 431 607
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 805 966 1038
Size: 805 161 72
Fragments arranged by size:
805 161 72
BseYI C'CCAG_C
Cuts at: 0 341 467 816 843 930 1038
Size: 341 126 349 27 87 108
Fragments arranged by size:
349 341 126 108 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 391 405 1038
Size: 187 204 14 633
Fragments arranged by size:
633 204 187 14
BsiHKAI G_wGCw'C
Cuts at: 0 548 953 1038
Size: 548 405 85
Fragments arranged by size:
548 405 85
Bsp1286I G_dGCh'C
Cuts at: 0 548 754 953 1038
Size: 548 206 199 85
Fragments arranged by size:
548 206 199 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 974 1038
Size: 974 64
BsrBI CCG'CTC
Cuts at: 0 219 1038
Size: 219 819
BsrFI r'CCGG_y
Cuts at: 0 896 1038
Size: 896 142
BstYI r'GATC_y
Cuts at: 0 9 533 1038
Size: 9 524 505
Fragments arranged by size:
524 505 9
BtgI C'CryG_G
Cuts at: 0 279 379 475 785 1038
Size: 279 100 96 310 253
Fragments arranged by size:
310 279 253 100 96
BtsI GCAGTG_nn'
Cuts at: 0 193 744 1038
Size: 193 551 294
Fragments arranged by size:
551 294 193
EaeI y'GGCC_r
Cuts at: 0 314 551 1038
Size: 314 237 487
Fragments arranged by size:
487 314 237
EarI CTCTTCn'nnn_
Cuts at: 0 100 905 1038
Size: 100 805 133
Fragments arranged by size:
805 133 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 672 1038
Size: 672 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 418 425 493 1038
Size: 108 310 7 68 545
Fragments arranged by size:
545 310 108 68 7
EcoO109I rG'GnC_Cy
Cuts at: 0 591 1025 1038
Size: 591 434 13
Fragments arranged by size:
591 434 13
FspI TGC'GCA
Cuts at: 0 484 1038
Size: 484 554
HaeII r_GCGC'y
Cuts at: 0 243 352 450 1038
Size: 243 109 98 588
Fragments arranged by size:
588 243 109 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1038
Size: 551 487
MspA1I CmG'CkG
Cuts at: 0 201 281 345 381 477 659 816 1038
Size: 201 80 64 36 96 182 157 222
Fragments arranged by size:
222 201 182 157 96 80 64 36
NcoI C'CATG_G
Cuts at: 0 785 1038
Size: 785 253
PasI CC'CwG_GG
Cuts at: 0 689 711 1038
Size: 689 22 327
Fragments arranged by size:
689 327 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 850 876 1038
Size: 850 26 162
Fragments arranged by size:
850 162 26
PfoI T'CCnGG_A
Cuts at: 0 495 1038
Size: 495 543
PmlI CAC'GTG
Cuts at: 0 549 1038
Size: 549 489
PpuMI rG'GwC_Cy
Cuts at: 0 591 1025 1038
Size: 591 434 13
Fragments arranged by size:
591 434 13
PstI C_TGCA'G
Cuts at: 0 200 356 455 707 1038
Size: 200 156 99 252 331
Fragments arranged by size:
331 252 200 156 99
PvuII CAG'CTG
Cuts at: 0 201 345 1038
Size: 201 144 693
Fragments arranged by size:
693 201 144
SacII CC_GC'GG
Cuts at: 0 282 382 478 1038
Size: 282 100 96 560
Fragments arranged by size:
560 282 100 96
SanDI GG'GwC_CC
Cuts at: 0 1025 1038
Size: 1025 13
SexAI A'CCwGG_T
Cuts at: 0 770 1038
Size: 770 268
SfcI C'TryA_G
Cuts at: 0 196 352 451 703 1038
Size: 196 156 99 252 335
Fragments arranged by size:
335 252 196 156 99
StuI AGG'CCT
Cuts at: 0 395 679 1038
Size: 395 284 359
Fragments arranged by size:
395 359 284
StyI C'CwwG_G
Cuts at: 0 785 849 1038
Size: 785 64 189
Fragments arranged by size:
785 189 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1038
Size: 477 32 529
Fragments arranged by size:
529 477 32
Tth111I GACn'n_nGTC
Cuts at: 0 1029 1038
Size: 1029 9
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AvaI BanII
BbsI BglI BglII BlpI BmrI BpmI Bpu10I BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BstYI BtgI BtsI EaeI EarI EciI
Eco57MI EcoO109I FspI HaeII MmeI MspA1I NcoI PasI
PflMI PfoI PmlI PpuMI PstI PvuII SacII SanDI
SexAI SfcI StuI StyI TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
XbaI XcmI XhoI XmaI XmnI ZraI