(Linear) (Six-base) MAPSORT of: KIEE1049.seq  Check: 7499  from: 1  to: 675



KIEE1049.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AccI GT'mk_AC

Cuts at:      0     281     675 
   Size:        281     394
 
AfeI AGC'GCT

Cuts at:      0     130     675 
   Size:        130     545
 
AvaI C'yCGr_G

Cuts at:      0      55     275     675 
   Size:         55     220     400

  Fragments arranged by size: 

                 400     220      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     213     660     675 
   Size:        213     447      15

  Fragments arranged by size: 

                 447     213      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     666     675 
   Size:        175     491       9

  Fragments arranged by size: 

                 491     175       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     634     675 
   Size:         52     582      41

  Fragments arranged by size: 

                 582      52      41
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      26     675 
   Size:         26     649
 
BseYI C'CCAG_C

Cuts at:      0      48     152     675 
   Size:         48     104     523

  Fragments arranged by size: 

                 523     104      48
 
BsiEI CG_ry'CG

Cuts at:      0      41     675 
   Size:         41     634
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     675 
   Size:         71     604
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     675 
   Size:         71     604
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     675 
   Size:        177     498
 
BstZ17I GTA'TAC

Cuts at:      0     282     675 
   Size:        282     393
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     228     675 
   Size:        228     447
 
EaeI y'GGCC_r

Cuts at:      0      38     675 
   Size:         38     637
 
EagI C'GGCC_G

Cuts at:      0      38     675 
   Size:         38     637
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     151     675 
   Size:         79      72     524

  Fragments arranged by size: 

                 524      79      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      23     675 
   Size:         23     652
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      76     675 
   Size:         76     599
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     195     675 
   Size:         21     174     480

  Fragments arranged by size: 

                 480     174      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     403     675 
   Size:        403     272
 
HaeII r_GCGC'y

Cuts at:      0      55     132     675 
   Size:         55      77     543

  Fragments arranged by size: 

                 543      77      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     646     675 
   Size:        646      29
 
HindIII A'AGCT_T

Cuts at:      0     591     675 
   Size:        591      84
 
MslI CAynn'nnrTG

Cuts at:      0     175     675 
   Size:        175     500
 
MspA1I CmG'CkG

Cuts at:      0     234     675 
   Size:        234     441
 
NotI GC'GGCC_GC

Cuts at:      0      38     675 
   Size:         38     637
 
PpuMI rG'GwC_Cy

Cuts at:      0      21     195     675 
   Size:         21     174     480

  Fragments arranged by size: 

                 480     174      21
 
PstI C_TGCA'G

Cuts at:      0     233     675 
   Size:        233     442
 
PvuII CAG'CTG

Cuts at:      0     234     675 
   Size:        234     441
 
SanDI GG'GwC_CC

Cuts at:      0      21     675 
   Size:         21     654
 
SapI GCTCTTCn'nnn_

Cuts at:      0     151     675 
   Size:        151     524
 
SfcI C'TryA_G

Cuts at:      0     229     675 
   Size:        229     446
 
SmlI C'TyrA_G

Cuts at:      0     190     645     675 
   Size:        190     455      30

  Fragments arranged by size: 

                 455     190      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174     675 
   Size:        174     501
 

 Enzymes that do cut:

     AccI     AfeI     AvaI     BmrI    BpuEI     BsaI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BstXI  BstZ17I    BtgZI     EaeI     EagI
     EarI     EciI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII
     MslI   MspA1I     NotI    PpuMI     PstI    PvuII    SanDI     SapI
     SfcI     SmlI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI