Restriction map for
pF1KE1050
(Linear) (Six-base) MAPSORT of: KIEE1050.seq Check: 6417 from: 1 to: 1428
KIEE1050.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1099 1428
Size: 1099 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1428
Size: 427 1001
AleI CACnn'nnGTG
Cuts at: 0 1093 1428
Size: 1093 335
AlwNI CAG_nnn'CTG
Cuts at: 0 1385 1428
Size: 1385 43
ApaI G_GGCC'C
Cuts at: 0 11 454 581 592 731 936 1428
Size: 11 443 127 11 139 205 492
Fragments arranged by size:
492 443 205 139 127 11 11
AvaI C'yCGr_G
Cuts at: 0 81 585 1428
Size: 81 504 843
Fragments arranged by size:
843 504 81
BamHI G'GATC_C
Cuts at: 0 1046 1428
Size: 1046 382
BanI G'GyrC_C
Cuts at: 0 76 735 1428
Size: 76 659 693
Fragments arranged by size:
693 659 76
BanII G_rGCy'C
Cuts at: 0 11 41 51 242 374 403 454 581
Size: 11 30 10 191 132 29 51 127
Cuts at: 581 592 731 936 1403 1428
Size: 11 139 205 467 25
Fragments arranged by size:
467 205 191 139 132 127 51 30
29 25 11 11 10
BglI GCCn_nnn'nGGC
Cuts at: 0 15 568 1118 1428
Size: 15 553 550 310
Fragments arranged by size:
553 550 310 15
BlpI GC'TnA_GC
Cuts at: 0 298 1428
Size: 298 1130
Bme1580I G_kGCm'C
Cuts at: 0 11 454 581 592 731 740 936 1361
Size: 11 443 127 11 139 9 196 425
Cuts at: 1361 1428
Size: 67
Fragments arranged by size:
443 425 196 139 127 67 11 11
9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 127 767 1428
Size: 127 640 661
Fragments arranged by size:
661 640 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 208 443 737 1027 1428
Size: 97 111 235 294 290 401
Fragments arranged by size:
401 294 290 235 111 97
Bpu10I CC'TnA_GC
Cuts at: 0 742 1223 1428
Size: 742 481 205
Fragments arranged by size:
742 481 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 693 1428
Size: 693 735
BsaAI yAC'GTr
Cuts at: 0 903 996 1022 1428
Size: 903 93 26 406
Fragments arranged by size:
903 406 93 26
BsaHI Gr'CG_yC
Cuts at: 0 281 1428
Size: 281 1147
BsaWI w'CCGG_w
Cuts at: 0 180 1043 1428
Size: 180 863 385
Fragments arranged by size:
863 385 180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 839 869 1428
Size: 839 30 559
Fragments arranged by size:
839 559 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 728 984 1252 1308 1312 1428
Size: 728 256 268 56 4 116
Fragments arranged by size:
728 268 256 116 56 4
BseYI C'CCAG_C
Cuts at: 0 432 495 682 811 871 935 1028 1428
Size: 432 63 187 129 60 64 93 400
Fragments arranged by size:
432 400 187 129 93 64 63 60
BsiHKAI G_wGCw'C
Cuts at: 0 725 1027 1403 1428
Size: 725 302 376 25
Fragments arranged by size:
725 376 302 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 443 1428
Size: 443 985
Bsp1286I G_dGCh'C
Cuts at: 0 11 41 51 242 374 403 454 581
Size: 11 30 10 191 132 29 51 127
Cuts at: 581 592 725 731 740 936 1027 1361 1403
Size: 11 133 6 9 196 91 334 42
Cuts at: 1403 1428
Size: 25
Fragments arranged by size:
334 196 191 133 132 127 91 51
42 30 29 25 11 11 10 9
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1099 1428
Size: 1099 329
BsrBI CCG'CTC
Cuts at: 0 429 1428
Size: 429 999
BsrFI r'CCGG_y
Cuts at: 0 93 195 1428
Size: 93 102 1233
Fragments arranged by size:
1233 102 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1376 1428
Size: 1376 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 838 1428
Size: 838 590
BstYI r'GATC_y
Cuts at: 0 1046 1151 1428
Size: 1046 105 277
Fragments arranged by size:
1046 277 105
Bsu36I CC'TnA_GG
Cuts at: 0 53 1428
Size: 53 1375
BtgI C'CryG_G
Cuts at: 0 72 99 213 962 1107 1428
Size: 72 27 114 749 145 321
Fragments arranged by size:
749 321 145 114 72 27
BtsI GCAGTG_nn'
Cuts at: 0 163 1155 1428
Size: 163 992 273
Fragments arranged by size:
992 273 163
EaeI y'GGCC_r
Cuts at: 0 311 828 945 998 1428
Size: 311 517 117 53 430
Fragments arranged by size:
517 430 311 117 53
EarI CTCTTCn'nnn_
Cuts at: 0 699 705 1428
Size: 699 6 723
Fragments arranged by size:
723 699 6
EcoICRI GAG'CTC
Cuts at: 0 1401 1428
Size: 1401 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 208 427 443 737 1027 1428
Size: 97 111 219 16 294 290 401
Fragments arranged by size:
401 294 290 219 111 97 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 605 1226 1428
Size: 605 621 202
Fragments arranged by size:
621 605 202
EcoO109I rG'GnC_Cy
Cuts at: 0 7 168 209 258 450 451 578 589
Size: 7 161 41 49 192 1 127 11
Cuts at: 589 628 728 1428
Size: 39 100 700
Fragments arranged by size:
700 192 161 127 100 49 41 39
11 7 1
HaeII r_GCGC'y
Cuts at: 0 1385 1428
Size: 1385 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 605 637 1428
Size: 605 32 791
Fragments arranged by size:
791 605 32
MscI TGG'CCA
Cuts at: 0 830 1000 1428
Size: 830 170 428
Fragments arranged by size:
830 428 170
MslI CAynn'nnrTG
Cuts at: 0 1093 1428
Size: 1093 335
MspA1I CmG'CkG
Cuts at: 0 74 815 1109 1350 1428
Size: 74 741 294 241 78
Fragments arranged by size:
741 294 241 78 74
NaeI GCC'GGC
Cuts at: 0 197 1428
Size: 197 1231
NcoI C'CATG_G
Cuts at: 0 213 1428
Size: 213 1215
NdeI CA'TA_TG
Cuts at: 0 366 1428
Size: 366 1062
NgoMIV G'CCGG_C
Cuts at: 0 195 1428
Size: 195 1233
NspI r_CATG'y
Cuts at: 0 929 1428
Size: 929 499
PasI CC'CwG_GG
Cuts at: 0 125 172 1428
Size: 125 47 1256
Fragments arranged by size:
1256 125 47
PfoI T'CCnGG_A
Cuts at: 0 1037 1394 1428
Size: 1037 357 34
Fragments arranged by size:
1037 357 34
PmlI CAC'GTG
Cuts at: 0 903 1022 1428
Size: 903 119 406
Fragments arranged by size:
903 406 119
PpuMI rG'GwC_Cy
Cuts at: 0 209 1428
Size: 209 1219
PspOMI G'GGCC_C
Cuts at: 0 7 450 577 588 727 932 1428
Size: 7 443 127 11 139 205 496
Fragments arranged by size:
496 443 205 139 127 11 7
PstI C_TGCA'G
Cuts at: 0 249 702 1381 1428
Size: 249 453 679 47
Fragments arranged by size:
679 453 249 47
SacI G_AGCT'C
Cuts at: 0 1403 1428
Size: 1403 25
SacII CC_GC'GG
Cuts at: 0 75 1110 1428
Size: 75 1035 318
Fragments arranged by size:
1035 318 75
SanDI GG'GwC_CC
Cuts at: 0 209 1428
Size: 209 1219
SbfI CC_TGCA'GG
Cuts at: 0 702 1428
Size: 702 726
ScaI AGT'ACT
Cuts at: 0 221 823 1428
Size: 221 602 605
Fragments arranged by size:
605 602 221
SfcI C'TryA_G
Cuts at: 0 245 636 698 1377 1428
Size: 245 391 62 679 51
Fragments arranged by size:
679 391 245 62 51
SmaI CCC'GGG
Cuts at: 0 83 587 1428
Size: 83 504 841
Fragments arranged by size:
841 504 83
StuI AGG'CCT
Cuts at: 0 571 1069 1375 1428
Size: 571 498 306 53
Fragments arranged by size:
571 498 306 53
StyI C'CwwG_G
Cuts at: 0 213 1428
Size: 213 1215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 192 371 564 1428
Size: 192 179 193 864
Fragments arranged by size:
864 193 192 179
TatI w'GTAC_w
Cuts at: 0 219 821 1428
Size: 219 602 607
Fragments arranged by size:
607 602 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1428
Size: 839 32 557
Fragments arranged by size:
839 557 32
XmaI C'CCGG_G
Cuts at: 0 81 585 1428
Size: 81 504 843
Fragments arranged by size:
843 504 81
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BamHI BanI
BanII BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI PstI
SacI SacII SanDI SbfI ScaI SfcI SmaI StuI
StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BpuEI BsaBI BsgI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SapI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmnI ZraI