Restriction map for
pF1KE1055
(Linear) (Six-base) MAPSORT of: KIEE1055.seq Check: 9646 from: 1 to: 4740
KIEE1055.orf
With 182 enzymes: SgfI *
October 31, 2008 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1535 2458 2981 4740
Size: 1535 923 523 1759
Fragments arranged by size:
1759 1535 923 523
AatII G_ACGT'C
Cuts at: 0 937 2513 4740
Size: 937 1576 2227
Fragments arranged by size:
2227 1576 937
Acc65I G'GTAC_C
Cuts at: 0 3962 4740
Size: 3962 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 901 968 1442 2878 3054 3121 3862
Size: 552 349 67 474 1436 176 67 741
Cuts at: 3862 4332 4740
Size: 470 408
Fragments arranged by size:
1436 741 552 474 470 408 349 176
67 67
AfeI AGC'GCT
Cuts at: 0 4646 4740
Size: 4646 94
AflIII A'CryG_T
Cuts at: 0 285 1878 4740
Size: 285 1593 2862
Fragments arranged by size:
2862 1593 285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3148 3446 3871 4061 4740
Size: 3148 298 425 190 679
Fragments arranged by size:
3148 679 425 298 190
AlwNI CAG_nnn'CTG
Cuts at: 0 3086 4740
Size: 3086 1654
ApaI G_GGCC'C
Cuts at: 0 2468 3377 4566 4740
Size: 2468 909 1189 174
Fragments arranged by size:
2468 1189 909 174
ApaLI G'TGCA_C
Cuts at: 0 1492 3797 4740
Size: 1492 2305 943
Fragments arranged by size:
2305 1492 943
ApoI r'AATT_y
Cuts at: 0 51 3300 3879 4720 4740
Size: 51 3249 579 841 20
Fragments arranged by size:
3249 841 579 51 20
AseI AT'TA_AT
Cuts at: 0 590 4740
Size: 590 4150
AvaI C'yCGr_G
Cuts at: 0 1343 1671 1956 2182 2481 2878 2890 2925
Size: 1343 328 285 226 299 397 12 35
Cuts at: 2925 3154 3394 3436 3451 3646 4002 4622 4740
Size: 229 240 42 15 195 356 620 118
Fragments arranged by size:
1343 620 397 356 328 299 285 240
229 226 195 118 42 35 15 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3946 3979 4740
Size: 3946 33 761
Fragments arranged by size:
3946 761 33
BamHI G'GATC_C
Cuts at: 0 1184 4740
Size: 1184 3556
BanI G'GyrC_C
Cuts at: 0 2966 3712 3808 3817 3962 4178 4740
Size: 2966 746 96 9 145 216 562
Fragments arranged by size:
2966 746 562 216 145 96 9
BanII G_rGCy'C
Cuts at: 0 203 299 359 670 1344 2016 2183 2468
Size: 203 96 60 311 674 672 167 285
Cuts at: 2468 2952 3377 4101 4566 4573 4740
Size: 484 425 724 465 7 167
Fragments arranged by size:
724 674 672 484 465 425 311 285
203 167 167 96 60 7
BbeI G_GCGC'C
Cuts at: 0 2970 4182 4740
Size: 2970 1212 558
Fragments arranged by size:
2970 1212 558
BbsI GAAGACnn'nnnn_
Cuts at: 0 1328 1620 1891 2123 2866 4740
Size: 1328 292 271 232 743 1874
Fragments arranged by size:
1874 1328 743 292 271 232
BbvCI CC'TCA_GC
Cuts at: 0 768 2035 3079 4740
Size: 768 1267 1044 1661
Fragments arranged by size:
1661 1267 1044 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2814 2848 4740
Size: 2814 34 1892
Fragments arranged by size:
2814 1892 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 512 4740
Size: 512 4228
BclI T'GATC_A
Cuts at: 0 254 4740
Size: 254 4486
BfrBI ATG'CAT
Cuts at: 0 1077 4740
Size: 1077 3663
BglI GCCn_nnn'nGGC
Cuts at: 0 3372 3416 3455 4740
Size: 3372 44 39 1285
Fragments arranged by size:
3372 1285 44 39
BglII A'GATC_T
Cuts at: 0 1702 4740
Size: 1702 3038
BlpI GC'TnA_GC
Cuts at: 0 202 664 2755 4740
Size: 202 462 2091 1985
Fragments arranged by size:
2091 1985 462 202
Bme1580I G_kGCm'C
Cuts at: 0 1496 2468 3368 3377 3801 4566 4740
Size: 1496 972 900 9 424 765 174
Fragments arranged by size:
1496 972 900 765 424 174 9
BmgBI CAC'GTC
Cuts at: 0 3110 3285 4740
Size: 3110 175 1455
Fragments arranged by size:
3110 1455 175
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 1458 1581 3943 4740
Size: 3 1455 123 2362 797
Fragments arranged by size:
2362 1455 797 123 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 4740
Size: 264 4476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 822 1733 2053 2598 2610 2700 2897
Size: 90 732 911 320 545 12 90 197
Cuts at: 2897 3244 4530 4740
Size: 347 1286 210
Fragments arranged by size:
1286 911 732 545 347 320 210 197
90 90 12
Bpu10I CC'TnA_GC
Cuts at: 0 768 1090 1107 2035 2575 3079 4567 4740
Size: 768 322 17 928 540 504 1488 173
Fragments arranged by size:
1488 928 768 540 504 322 173 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1056 1545 1719 2770 3514 3633 4740
Size: 1056 489 174 1051 744 119 1107
Fragments arranged by size:
1107 1056 1051 744 489 174 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 2210 2415 4740
Size: 2210 205 2325
Fragments arranged by size:
2325 2210 205
BsaAI yAC'GTr
Cuts at: 0 288 500 2331 4740
Size: 288 212 1831 2409
Fragments arranged by size:
2409 1831 288 212
BsaHI Gr'CG_yC
Cuts at: 0 934 2510 2635 2702 2827 2967 4179 4391
Size: 934 1576 125 67 125 140 1212 212
Cuts at: 4391 4627 4740
Size: 236 113
Fragments arranged by size:
1576 1212 934 236 212 140 125 125
113 67
BsaWI w'CCGG_w
Cuts at: 0 504 536 1871 3499 4740
Size: 504 32 1335 1628 1241
Fragments arranged by size:
1628 1335 1241 504 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 1525 1555 1849 1879 2677 2707 3502
Size: 25 1500 30 294 30 798 30 795
Cuts at: 3502 3532 4740
Size: 30 1208
Fragments arranged by size:
1500 1208 798 795 294 30 30 30
30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 2405 2580 2943 3021 3510 3630 3970
Size: 362 2043 175 363 78 489 120 340
Cuts at: 3970 4593 4740
Size: 623 147
Fragments arranged by size:
2043 623 489 363 362 340 175 147
120 78
BseYI C'CCAG_C
Cuts at: 0 699 1330 2746 2771 3199 3337 3628 4641
Size: 699 631 1416 25 428 138 291 1013
Cuts at: 4641 4740
Size: 99
Fragments arranged by size:
1416 1013 699 631 428 291 138 99
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1563 1824 2955 3310 4740
Size: 1563 261 1131 355 1430
Fragments arranged by size:
1563 1430 1131 355 261
BsiEI CG_ry'CG
Cuts at: 0 1948 2002 2656 4740
Size: 1948 54 654 2084
Fragments arranged by size:
2084 1948 654 54
BsiHKAI G_wGCw'C
Cuts at: 0 70 1496 1739 2330 3683 3801 3850 4300
Size: 70 1426 243 591 1353 118 49 450
Cuts at: 4300 4660 4740
Size: 360 80
Fragments arranged by size:
1426 1353 591 450 360 243 118 80
70 49
BsmI GAATG_Cn'
Cuts at: 0 1800 2068 4740
Size: 1800 268 2672
Fragments arranged by size:
2672 1800 268
BsmBI CGTCTCn'nnnn_
Cuts at: 0 941 1068 4740
Size: 941 127 3672
Fragments arranged by size:
3672 941 127
Bsp1286I G_dGCh'C
Cuts at: 0 70 203 299 359 670 1344 1496 1739
Size: 70 133 96 60 311 674 152 243
Cuts at: 1739 2016 2183 2330 2468 2952 3368 3377 3683
Size: 277 167 147 138 484 416 9 306
Cuts at: 3683 3801 3850 4101 4300 4566 4573 4660 4740
Size: 118 49 251 199 266 7 87 80
Fragments arranged by size:
674 484 416 311 306 277 266 251
243 199 167 152 147 138 133 118
96 87 80 70 60 49 9 7
BspEI T'CCGG_A
Cuts at: 0 504 1871 3499 4740
Size: 504 1367 1628 1241
Fragments arranged by size:
1628 1367 1241 504
BspHI T'CATG_A
Cuts at: 0 423 4740
Size: 423 4317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 518 1535 2458 2981 3479 4252 4740
Size: 518 1017 923 523 498 773 488
Fragments arranged by size:
1017 923 773 523 518 498 488
BsrBI CCG'CTC
Cuts at: 0 614 2588 2624 2714 3620 3866 4740
Size: 614 1974 36 90 906 246 874
Fragments arranged by size:
1974 906 874 614 246 90 36
BsrDI GCAATG_nn'
Cuts at: 0 463 4740
Size: 463 4277
BsrFI r'CCGG_y
Cuts at: 0 781 2651 3891 4740
Size: 781 1870 1240 849
Fragments arranged by size:
1870 1240 849 781
BssSI C'ACGA_G
Cuts at: 0 1251 3056 3130 4740
Size: 1251 1805 74 1610
Fragments arranged by size:
1805 1610 1251 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1491 3687 4740
Size: 1491 2196 1053
Fragments arranged by size:
2196 1491 1053
BstEII G'GTnAC_C
Cuts at: 0 1279 4740
Size: 1279 3461
BstXI CCAn_nnnn'nTGG
Cuts at: 0 801 948 1519 2322 4668 4740
Size: 801 147 571 803 2346 72
Fragments arranged by size:
2346 803 801 571 147 72
BstYI r'GATC_y
Cuts at: 0 266 524 1085 1184 1702 2597 4740
Size: 266 258 561 99 518 895 2143
Fragments arranged by size:
2143 895 561 518 266 258 99
Bsu36I CC'TnA_GG
Cuts at: 0 2592 4740
Size: 2592 2148
BtgI C'CryG_G
Cuts at: 0 270 312 329 657 2952 2997 3113 3236
Size: 270 42 17 328 2295 45 116 123
Cuts at: 3236 3369 3541 4340 4740
Size: 133 172 799 400
Fragments arranged by size:
2295 799 400 328 270 172 133 123
116 45 42 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 617 3115 4740
Size: 617 2498 1625
Fragments arranged by size:
2498 1625 617
BtsI GCAGTG_nn'
Cuts at: 0 1271 2264 4740
Size: 1271 993 2476
Fragments arranged by size:
2476 1271 993
ClaI AT'CG_AT
Cuts at: 0 2231 4740
Size: 2231 2509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2262 2297 4451 4486 4740
Size: 2262 35 2154 35 254
Fragments arranged by size:
2262 2154 254 35 35
DraI TTT'AAA
Cuts at: 0 3299 4740
Size: 3299 1441
DraIII CAC_nnn'GTG
Cuts at: 0 3003 4740
Size: 3003 1737
EaeI y'GGCC_r
Cuts at: 0 735 1945 1995 2653 2672 3104 3162 3417
Size: 735 1210 50 658 19 432 58 255
Cuts at: 3417 4740
Size: 1323
Fragments arranged by size:
1323 1210 735 658 432 255 58 50
19
EagI C'GGCC_G
Cuts at: 0 1945 2653 4740
Size: 1945 708 2087
Fragments arranged by size:
2087 1945 708
EarI CTCTTCn'nnn_
Cuts at: 0 987 1056 3838 4740
Size: 987 69 2782 902
Fragments arranged by size:
2782 987 902 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 307 468 4740
Size: 307 161 4272
Fragments arranged by size:
4272 307 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 552 822 901 968 1442 1733 2053
Size: 90 462 270 79 67 474 291 320
Cuts at: 2053 2598 2610 2700 2878 2897 3054 3121 3244
Size: 545 12 90 178 19 157 67 123
Cuts at: 3244 3862 4332 4530 4740
Size: 618 470 198 210
Fragments arranged by size:
618 545 474 470 462 320 291 270
210 198 178 157 123 90 90 79
67 67 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 519 1178 1381 2420 4405 4740
Size: 519 659 203 1039 1985 335
Fragments arranged by size:
1985 1039 659 519 335 203
EcoO109I rG'GnC_Cy
Cuts at: 0 245 275 2031 3042 3750 3945 4562 4563
Size: 245 30 1756 1011 708 195 617 1
Cuts at: 4563 4740
Size: 177
Fragments arranged by size:
1756 1011 708 617 245 195 177 30
1
EcoRI G'AATT_C
Cuts at: 0 51 4740
Size: 51 4689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1504 4740
Size: 1504 3236
FseI GG_CCGG'CC
Cuts at: 0 2655 4740
Size: 2655 2085
HaeII r_GCGC'y
Cuts at: 0 2552 2875 2970 3344 3509 3901 4182 4421
Size: 2552 323 95 374 165 392 281 239
Cuts at: 4421 4648 4740
Size: 227 92
Fragments arranged by size:
2552 392 374 323 281 239 227 165
95 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1849 1881 1919 1951 2677 2709 3502
Size: 27 1822 32 38 32 726 32 793
Cuts at: 3502 3534 4740
Size: 32 1206
Fragments arranged by size:
1822 1206 793 726 38 32 32 32
32 27
HindIII A'AGCT_T
Cuts at: 0 4125 4740
Size: 4125 615
KasI G'GCGC_C
Cuts at: 0 2966 4178 4740
Size: 2966 1212 562
Fragments arranged by size:
2966 1212 562
KpnI G_GTAC'C
Cuts at: 0 3966 4740
Size: 3966 774
MfeI C'AATT_G
Cuts at: 0 407 4740
Size: 407 4333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 2195 2465 4740
Size: 1186 1009 270 2275
Fragments arranged by size:
2275 1186 1009 270
MscI TGG'CCA
Cuts at: 0 737 3106 3419 4740
Size: 737 2369 313 1321
Fragments arranged by size:
2369 1321 737 313
MslI CAynn'nnrTG
Cuts at: 0 799 1517 1805 1821 3853 4666 4740
Size: 799 718 288 16 2032 813 74
Fragments arranged by size:
2032 813 799 718 288 74 16
MspA1I CmG'CkG
Cuts at: 0 314 547 772 3086 3115 3543 3550 3876
Size: 314 233 225 2314 29 428 7 326
Cuts at: 3876 3913 4293 4397 4740
Size: 37 380 104 343
Fragments arranged by size:
2314 428 380 343 326 314 233 225
104 37 29 7
NaeI GCC'GGC
Cuts at: 0 783 2653 4740
Size: 783 1870 2087
Fragments arranged by size:
2087 1870 783
NarI GG'CG_CC
Cuts at: 0 2967 4179 4740
Size: 2967 1212 561
Fragments arranged by size:
2967 1212 561
NcoI C'CATG_G
Cuts at: 0 270 329 3236 4740
Size: 270 59 2907 1504
Fragments arranged by size:
2907 1504 270 59
NdeI CA'TA_TG
Cuts at: 0 470 1607 1818 4740
Size: 470 1137 211 2922
Fragments arranged by size:
2922 1137 470 211
NgoMIV G'CCGG_C
Cuts at: 0 781 2651 4740
Size: 781 1870 2089
Fragments arranged by size:
2089 1870 781
NsiI A_TGCA'T
Cuts at: 0 1079 4740
Size: 1079 3661
NspI r_CATG'y
Cuts at: 0 1077 1882 2529 3936 3996 4740
Size: 1077 805 647 1407 60 744
Fragments arranged by size:
1407 1077 805 744 647 60
PasI CC'CwG_GG
Cuts at: 0 1336 4740
Size: 1336 3404
PciI A'CATG_T
Cuts at: 0 1878 4740
Size: 1878 2862
PflMI CCAn_nnn'nTGG
Cuts at: 0 2119 3482 4341 4494 4740
Size: 2119 1363 859 153 246
Fragments arranged by size:
2119 1363 859 246 153
PfoI T'CCnGG_A
Cuts at: 0 135 3347 4740
Size: 135 3212 1393
Fragments arranged by size:
3212 1393 135
PmlI CAC'GTG
Cuts at: 0 288 4740
Size: 288 4452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1095 1127 4740
Size: 1095 32 3613
Fragments arranged by size:
3613 1095 32
PpuMI rG'GwC_Cy
Cuts at: 0 275 2031 3945 4740
Size: 275 1756 1914 795
Fragments arranged by size:
1914 1756 795 275
PshAI GACnn'nnGTC
Cuts at: 0 4235 4740
Size: 4235 505
PspOMI G'GGCC_C
Cuts at: 0 2464 3373 4562 4740
Size: 2464 909 1189 178
Fragments arranged by size:
2464 1189 909 178
PspXI vC'TCGA_Gb
Cuts at: 0 2182 2890 3436 4740
Size: 2182 708 546 1304
Fragments arranged by size:
2182 1304 708 546
PstI C_TGCA'G
Cuts at: 0 1266 2455 2978 4214 4249 4310 4740
Size: 1266 1189 523 1236 35 61 430
Fragments arranged by size:
1266 1236 1189 523 430 61 35
PvuII CAG'CTG
Cuts at: 0 772 3086 3876 3913 4397 4740
Size: 772 2314 790 37 484 343
Fragments arranged by size:
2314 790 772 484 343 37
SacII CC_GC'GG
Cuts at: 0 315 3116 3544 4740
Size: 315 2801 428 1196
Fragments arranged by size:
2801 1196 428 315
SanDI GG'GwC_CC
Cuts at: 0 275 4740
Size: 275 4465
SapI GCTCTTCn'nnn_
Cuts at: 0 987 3838 4740
Size: 987 2851 902
Fragments arranged by size:
2851 987 902
SbfI CC_TGCA'GG
Cuts at: 0 4249 4740
Size: 4249 491
SfcI C'TryA_G
Cuts at: 0 671 1262 2451 2974 4210 4245 4306 4405
Size: 671 591 1189 523 1236 35 61 99
Cuts at: 4405 4740
Size: 335
Fragments arranged by size:
1236 1189 671 591 523 335 99 61
35
SfoI GGC'GCC
Cuts at: 0 2968 4180 4740
Size: 2968 1212 560
Fragments arranged by size:
2968 1212 560
SmaI CCC'GGG
Cuts at: 0 2483 3396 4004 4740
Size: 2483 913 608 736
Fragments arranged by size:
2483 913 736 608
SmlI C'TyrA_G
Cuts at: 0 1035 1560 1671 1698 2182 2785 2890 3436
Size: 1035 525 111 27 484 603 105 546
Cuts at: 3436 3529 3612 4740
Size: 93 83 1128
Fragments arranged by size:
1128 1035 603 546 525 484 111 105
93 83 27
SnaBI TAC'GTA
Cuts at: 0 500 4740
Size: 500 4240
SphI G_CATG'C
Cuts at: 0 3936 3996 4740
Size: 3936 60 744
Fragments arranged by size:
3936 744 60
SrfI GCCC'GGGC
Cuts at: 0 3396 4740
Size: 3396 1344
StuI AGG'CCT
Cuts at: 0 1173 1666 4740
Size: 1173 493 3074
Fragments arranged by size:
3074 1173 493
StyI C'CwwG_G
Cuts at: 0 270 329 947 2016 3236 4071 4740
Size: 270 59 618 1069 1220 835 669
Fragments arranged by size:
1220 1069 835 669 618 270 59
SwaI ATTT'AAAT
Cuts at: 0 3299 4740
Size: 3299 1441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1492 2016 2212 2449 4740
Size: 1492 524 196 237 2291
Fragments arranged by size:
2291 1492 524 237 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 176 208 1525 1557 4740
Size: 176 32 1317 32 3183
Fragments arranged by size:
3183 1317 176 32 32
XhoI C'TCGA_G
Cuts at: 0 1671 2182 2890 3436 4740
Size: 1671 511 708 546 1304
Fragments arranged by size:
1671 1304 708 546 511
XmaI C'CCGG_G
Cuts at: 0 2481 3394 4002 4740
Size: 2481 913 608 738
Fragments arranged by size:
2481 913 738 608
XmnI GAAnn'nnTTC
Cuts at: 0 809 1534 1576 1800 4740
Size: 809 725 42 224 2940
Fragments arranged by size:
2940 809 725 224 42
ZraI GAC'GTC
Cuts at: 0 935 2511 4740
Size: 935 1576 2229
Fragments arranged by size:
2229 1576 935
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI HaeII Hin4I HindIII KasI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacII SanDI SapI SbfI SfcI SfoI SmaI
SmlI SnaBI SphI SrfI StuI StyI SwaI TaqII
TstI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AleI AloI AscI
AsiSI AvrII BmtI BsaBI BsiWI BsrGI BssHII BstBI
BstZ17I DrdI EcoICRI EcoRV FspI FspAI HincII HpaI
MluI NheI NotI NruI PacI PmeI PsiI PsrI
PvuI RsrII SacI SalI ScaI SexAI SfiI SgfI
SgrAI SpeI SspI TatI Tth111I XbaI XcmI