(Linear) (Six-base) MAPSORT of: KIEE1065.seq Check: 8845 from: 1 to: 2964 KIEE1065.orf With 182 enzymes: SgfI * February 26, 2009 15:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 967 2964 Size: 967 1997 AccI GT'mk_AC Cuts at: 0 95 2964 Size: 95 2869 AflIII A'CryG_T Cuts at: 0 673 2036 2964 Size: 673 1363 928 Fragments arranged by size: 1363 928 673 AleI CACnn'nnGTG Cuts at: 0 647 784 1916 2964 Size: 647 137 1132 1048 Fragments arranged by size: 1132 1048 647 137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1399 2964 Size: 1399 1565 AlwNI CAG_nnn'CTG Cuts at: 0 781 1038 1542 1731 1801 2964 Size: 781 257 504 189 70 1163 Fragments arranged by size: 1163 781 504 257 189 70 ApaI G_GGCC'C Cuts at: 0 1251 1421 2964 Size: 1251 170 1543 Fragments arranged by size: 1543 1251 170 ApaLI G'TGCA_C Cuts at: 0 2221 2964 Size: 2221 743 ApoI r'AATT_y Cuts at: 0 2804 2964 Size: 2804 160 AvaI C'yCGr_G Cuts at: 0 282 1020 1167 1244 1414 2134 2296 2964 Size: 282 738 147 77 170 720 162 668 Fragments arranged by size: 738 720 668 282 170 162 147 77 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 282 315 2964 Size: 282 33 2649 Fragments arranged by size: 2649 282 33 BamHI G'GATC_C Cuts at: 0 2504 2666 2964 Size: 2504 162 298 Fragments arranged by size: 2504 298 162 BanI G'GyrC_C Cuts at: 0 1399 1601 1906 2026 2188 2284 2774 2964 Size: 1399 202 305 120 162 96 490 190 Fragments arranged by size: 1399 490 305 202 190 162 120 96 BanII G_rGCy'C Cuts at: 0 83 1251 1421 1928 2135 2964 Size: 83 1168 170 507 207 829 Fragments arranged by size: 1168 829 507 207 170 83 BbeI G_GCGC'C Cuts at: 0 1605 2778 2964 Size: 1605 1173 186 Fragments arranged by size: 1605 1173 186 BbsI GAAGACnn'nnnn_ Cuts at: 0 224 838 2964 Size: 224 614 2126 Fragments arranged by size: 2126 614 224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 705 739 779 813 1746 1780 1896 1930 Size: 705 34 40 34 933 34 116 34 Cuts at: 1930 2331 2365 2964 Size: 401 34 599 Fragments arranged by size: 933 705 599 401 116 40 34 34 34 34 34 BclI T'GATC_A Cuts at: 0 2825 2964 Size: 2825 139 BglI GCCn_nnn'nGGC Cuts at: 0 434 929 2114 2282 2964 Size: 434 495 1185 168 682 Fragments arranged by size: 1185 682 495 434 168 BglII A'GATC_T Cuts at: 0 389 2525 2964 Size: 389 2136 439 Fragments arranged by size: 2136 439 389 Bme1580I G_kGCm'C Cuts at: 0 52 121 522 1251 1421 1999 2031 2119 Size: 52 69 401 729 170 578 32 88 Cuts at: 2119 2225 2717 2964 Size: 106 492 247 Fragments arranged by size: 729 578 492 401 247 170 106 88 69 52 32 BmgBI CAC'GTC Cuts at: 0 99 731 2591 2844 2964 Size: 99 632 1860 253 120 Fragments arranged by size: 1860 632 253 120 99 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2600 2964 Size: 2600 364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1858 2048 2964 Size: 1858 190 916 Fragments arranged by size: 1858 916 190 Bpu10I CC'TnA_GC Cuts at: 0 1348 2129 2964 Size: 1348 781 835 Fragments arranged by size: 1348 835 781 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1561 2964 Size: 1561 1403 BsaI GGTCTCn'nnnn_ Cuts at: 0 1519 1797 2602 2964 Size: 1519 278 805 362 Fragments arranged by size: 1519 805 362 278 BsaAI yAC'GTr Cuts at: 0 674 719 2120 2964 Size: 674 45 1401 844 Fragments arranged by size: 1401 844 674 45 BsaBI GATnn'nnATC Cuts at: 0 2509 2964 Size: 2509 455 BsaHI Gr'CG_yC Cuts at: 0 538 1602 2775 2964 Size: 538 1064 1173 189 Fragments arranged by size: 1173 1064 538 189 BsaWI w'CCGG_w Cuts at: 0 758 2964 Size: 758 2206 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 964 994 2964 Size: 964 30 1970 Fragments arranged by size: 1970 964 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1578 2964 Size: 1578 1386 BseYI C'CCAG_C Cuts at: 0 1250 1420 1999 2454 2964 Size: 1250 170 579 455 510 Fragments arranged by size: 1250 579 510 455 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 588 1116 2964 Size: 588 528 1848 Fragments arranged by size: 1848 588 528 BsiEI CG_ry'CG Cuts at: 0 14 714 759 1770 2081 2964 Size: 14 700 45 1011 311 883 Fragments arranged by size: 1011 883 700 311 45 14 BsiHKAI G_wGCw'C Cuts at: 0 112 982 1928 2225 2964 Size: 112 870 946 297 739 Fragments arranged by size: 946 870 739 297 112 BsmI GAATG_Cn' Cuts at: 0 2473 2964 Size: 2473 491 BsmBI CGTCTCn'nnnn_ Cuts at: 0 529 721 2964 Size: 529 192 2243 Fragments arranged by size: 2243 529 192 Bsp1286I G_dGCh'C Cuts at: 0 52 83 112 121 522 982 1251 1421 Size: 52 31 29 9 401 460 269 170 Cuts at: 1421 1928 1999 2031 2119 2135 2225 2717 2964 Size: 507 71 32 88 16 90 492 247 Fragments arranged by size: 507 492 460 401 269 247 170 90 88 71 52 32 31 29 16 9 BspHI T'CATG_A Cuts at: 0 2567 2964 Size: 2567 397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 310 472 967 2620 2964 Size: 310 162 495 1653 344 Fragments arranged by size: 1653 495 344 310 162 BsrBI CCG'CTC Cuts at: 0 476 990 2651 2964 Size: 476 514 1661 313 Fragments arranged by size: 1661 514 476 313 BsrFI r'CCGG_y Cuts at: 0 1769 2143 2416 2964 Size: 1769 374 273 548 Fragments arranged by size: 1769 548 374 273 BssSI C'ACGA_G Cuts at: 0 265 1514 2964 Size: 265 1249 1450 Fragments arranged by size: 1450 1249 265 BstAPI GCAn_nnn'nTGC Cuts at: 0 1542 1994 2147 2273 2964 Size: 1542 452 153 126 691 Fragments arranged by size: 1542 691 452 153 126 BstXI CCAn_nnnn'nTGG Cuts at: 0 1836 1918 2964 Size: 1836 82 1046 Fragments arranged by size: 1836 1046 82 BstYI r'GATC_y Cuts at: 0 389 1472 2504 2525 2666 2964 Size: 389 1083 1032 21 141 298 Fragments arranged by size: 1083 1032 389 298 141 21 BtgI C'CryG_G Cuts at: 0 404 510 899 1047 1605 2111 2905 2964 Size: 404 106 389 148 558 506 794 59 Fragments arranged by size: 794 558 506 404 389 148 106 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 804 2466 2570 2619 2783 2964 Size: 804 1662 104 49 164 181 Fragments arranged by size: 1662 804 181 164 104 49 BtsI GCAGTG_nn' Cuts at: 0 217 814 1074 1551 2223 2313 2373 2388 Size: 217 597 260 477 672 90 60 15 Cuts at: 2388 2964 Size: 576 Fragments arranged by size: 672 597 576 477 260 217 90 60 15 ClaI AT'CG_AT Cuts at: 0 137 2964 Size: 137 2827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 357 392 2964 Size: 357 35 2572 Fragments arranged by size: 2572 357 35 DraIII CAC_nnn'GTG Cuts at: 0 1994 2964 Size: 1994 970 EaeI y'GGCC_r Cuts at: 0 11 65 1608 1767 1929 2078 2235 2304 Size: 11 54 1543 159 162 149 157 69 Cuts at: 2304 2964 Size: 660 Fragments arranged by size: 1543 660 162 159 157 149 69 54 11 EagI C'GGCC_G Cuts at: 0 11 1767 2078 2964 Size: 11 1756 311 886 Fragments arranged by size: 1756 886 311 11 EarI CTCTTCn'nnn_ Cuts at: 0 1321 1591 1670 2569 2842 2964 Size: 1321 270 79 899 273 122 Fragments arranged by size: 1321 899 273 270 122 79 EcoICRI GAG'CTC Cuts at: 0 1926 2964 Size: 1926 1038 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1858 2048 2964 Size: 1858 190 916 Fragments arranged by size: 1858 916 190 EcoNI CCTnn'n_nnAGG Cuts at: 0 2625 2964 Size: 2625 339 EcoO109I rG'GnC_Cy Cuts at: 0 667 2832 2964 Size: 667 2165 132 Fragments arranged by size: 2165 667 132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1482 2853 2964 Size: 1482 1371 111 Fragments arranged by size: 1482 1371 111 FspI TGC'GCA Cuts at: 0 735 1756 2964 Size: 735 1021 1208 Fragments arranged by size: 1208 1021 735 HaeII r_GCGC'y Cuts at: 0 1384 1605 1837 2778 2964 Size: 1384 221 232 941 186 Fragments arranged by size: 1384 941 232 221 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 94 126 964 996 2964 Size: 94 32 838 32 1968 Fragments arranged by size: 1968 838 94 32 32 HincII GTy'rAC Cuts at: 0 96 864 1134 2964 Size: 96 768 270 1830 Fragments arranged by size: 1830 768 270 96 KasI G'GCGC_C Cuts at: 0 1601 2774 2964 Size: 1601 1173 190 Fragments arranged by size: 1601 1173 190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 786 2021 2964 Size: 786 1235 943 Fragments arranged by size: 1235 943 786 MscI TGG'CCA Cuts at: 0 2306 2964 Size: 2306 658 MslI CAynn'nnrTG Cuts at: 0 137 515 647 784 1556 1916 2964 Size: 137 378 132 137 772 360 1048 Fragments arranged by size: 1048 772 378 360 137 137 132 MspA1I CmG'CkG Cuts at: 0 77 647 797 1049 1254 1424 1542 2264 Size: 77 570 150 252 205 170 118 722 Cuts at: 2264 2273 2696 2964 Size: 9 423 268 Fragments arranged by size: 722 570 423 268 252 205 170 150 118 77 9 NaeI GCC'GGC Cuts at: 0 1771 2964 Size: 1771 1193 NarI GG'CG_CC Cuts at: 0 1602 2775 2964 Size: 1602 1173 189 Fragments arranged by size: 1602 1173 189 NcoI C'CATG_G Cuts at: 0 404 510 2964 Size: 404 106 2454 Fragments arranged by size: 2454 404 106 NgoMIV G'CCGG_C Cuts at: 0 1769 2964 Size: 1769 1195 NspI r_CATG'y Cuts at: 0 767 839 1694 1856 2964 Size: 767 72 855 162 1108 Fragments arranged by size: 1108 855 767 162 72 PasI CC'CwG_GG Cuts at: 0 45 85 2964 Size: 45 40 2879 Fragments arranged by size: 2879 45 40 PflMI CCAn_nnn'nTGG Cuts at: 0 1968 2964 Size: 1968 996 PfoI T'CCnGG_A Cuts at: 0 2201 2964 Size: 2201 763 PmlI CAC'GTG Cuts at: 0 674 719 2964 Size: 674 45 2245 Fragments arranged by size: 2245 674 45 PshAI GACnn'nnGTC Cuts at: 0 359 2964 Size: 359 2605 PspOMI G'GGCC_C Cuts at: 0 1247 1417 2964 Size: 1247 170 1547 Fragments arranged by size: 1547 1247 170 PstI C_TGCA'G Cuts at: 0 568 821 1264 1388 1736 2964 Size: 568 253 443 124 348 1228 Fragments arranged by size: 1228 568 443 348 253 124 PvuII CAG'CTG Cuts at: 0 647 797 1254 1424 2964 Size: 647 150 457 170 1540 Fragments arranged by size: 1540 647 457 170 150 SacI G_AGCT'C Cuts at: 0 1928 2964 Size: 1928 1036 SacII CC_GC'GG Cuts at: 0 1050 2964 Size: 1050 1914 SalI G'TCGA_C Cuts at: 0 94 2964 Size: 94 2870 SbfI CC_TGCA'GG Cuts at: 0 1736 2964 Size: 1736 1228 SfcI C'TryA_G Cuts at: 0 291 564 817 1260 1384 1732 1873 2964 Size: 291 273 253 443 124 348 141 1091 Fragments arranged by size: 1091 443 348 291 273 253 141 124 SfoI GGC'GCC Cuts at: 0 1603 2776 2964 Size: 1603 1173 188 Fragments arranged by size: 1603 1173 188 SmaI CCC'GGG Cuts at: 0 284 1022 1169 1246 2136 2964 Size: 284 738 147 77 890 828 Fragments arranged by size: 890 828 738 284 147 77 SmlI C'TyrA_G Cuts at: 0 1576 2964 Size: 1576 1388 SrfI GCCC'GGGC Cuts at: 0 2136 2964 Size: 2136 828 StuI AGG'CCT Cuts at: 0 1681 2104 2964 Size: 1681 423 860 Fragments arranged by size: 1681 860 423 StyI C'CwwG_G Cuts at: 0 404 510 1139 1625 1829 2891 2964 Size: 404 106 629 486 204 1062 73 Fragments arranged by size: 1062 629 486 404 204 106 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1924 2272 2964 Size: 1924 348 692 Fragments arranged by size: 1924 692 348 TatI w'GTAC_w Cuts at: 0 2873 2964 Size: 2873 91 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 549 2299 2964 Size: 549 1750 665 Fragments arranged by size: 1750 665 549 XmaI C'CCGG_G Cuts at: 0 282 1020 1167 1244 2134 2964 Size: 282 738 147 77 890 830 Fragments arranged by size: 890 830 738 282 147 77 XmnI GAAnn'nnTTC Cuts at: 0 2866 2964 Size: 2866 98 Enzymes that do cut: AarI AccI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PshAI PspOMI PstI PvuII SacI SacII SalI SbfI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI AscI AseI AsiSI AvrII BbvCI BciVI BfrBI BlpI BmrI BmtI BsiWI BspEI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I DraI DrdI EciI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI ZraI