Restriction map for
pF1KE1066
(Linear) (Six-base) MAPSORT of: KIEE1066.seq Check: 2956 from: 1 to: 1476
KIEE1066.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1399 1476
Size: 1399 77
AccI GT'mk_AC
Cuts at: 0 194 1476
Size: 194 1282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 650 734 1476
Size: 434 216 84 742
Fragments arranged by size:
742 434 216 84
AfeI AGC'GCT
Cuts at: 0 124 1476
Size: 124 1352
AflII C'TTAA_G
Cuts at: 0 253 546 1476
Size: 253 293 930
Fragments arranged by size:
930 293 253
AhdI GACnn_n'nnGTC
Cuts at: 0 748 1476
Size: 748 728
ApaI G_GGCC'C
Cuts at: 0 56 1181 1476
Size: 56 1125 295
Fragments arranged by size:
1125 295 56
ApoI r'AATT_y
Cuts at: 0 271 408 997 1027 1092 1216 1476
Size: 271 137 589 30 65 124 260
Fragments arranged by size:
589 271 260 137 124 65 30
AvaI C'yCGr_G
Cuts at: 0 26 68 1034 1476
Size: 26 42 966 442
Fragments arranged by size:
966 442 42 26
AvrII C'CTAG_G
Cuts at: 0 1164 1476
Size: 1164 312
BamHI G'GATC_C
Cuts at: 0 1224 1476
Size: 1224 252
BanI G'GyrC_C
Cuts at: 0 470 1476
Size: 470 1006
BanII G_rGCy'C
Cuts at: 0 27 56 602 1181 1476
Size: 27 29 546 579 295
Fragments arranged by size:
579 546 295 29 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 656 1476
Size: 656 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1418 1452 1476
Size: 1418 34 24
Fragments arranged by size:
1418 34 24
Bme1580I G_kGCm'C
Cuts at: 0 56 1181 1297 1476
Size: 56 1125 116 179
Fragments arranged by size:
1125 179 116 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 5 1311 1338 1476
Size: 5 1306 27 138
Fragments arranged by size:
1306 138 27 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 621 1476
Size: 621 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 675 1259 1476
Size: 55 620 584 217
Fragments arranged by size:
620 584 217 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1476
Size: 1222 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 641 1476
Size: 641 835
BsaAI yAC'GTr
Cuts at: 0 111 1476
Size: 111 1365
BsaBI GATnn'nnATC
Cuts at: 0 968 1476
Size: 968 508
BsaHI Gr'CG_yC
Cuts at: 0 4 1381 1476
Size: 4 1377 95
Fragments arranged by size:
1377 95 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1476
Size: 91 30 1355
Fragments arranged by size:
1355 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 865 1219 1413 1476
Size: 865 354 194 63
Fragments arranged by size:
865 354 194 63
BseYI C'CCAG_C
Cuts at: 0 842 1476
Size: 842 634
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 1476
Size: 1427 49
BsiEI CG_ry'CG
Cuts at: 0 61 207 213 1476
Size: 61 146 6 1263
Fragments arranged by size:
1263 146 61 6
BsiHKAI G_wGCw'C
Cuts at: 0 465 602 1375 1476
Size: 465 137 773 101
Fragments arranged by size:
773 465 137 101
BsmI GAATG_Cn'
Cuts at: 0 1059 1476
Size: 1059 417
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 1476
Size: 11 1465
Bsp1286I G_dGCh'C
Cuts at: 0 27 56 465 602 1181 1297 1375 1476
Size: 27 29 409 137 579 116 78 101
Fragments arranged by size:
579 409 137 116 101 78 29 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 1160 1399 1476
Size: 169 991 239 77
Fragments arranged by size:
991 239 169 77
BsrBI CCG'CTC
Cuts at: 0 63 182 1256 1476
Size: 63 119 1074 220
Fragments arranged by size:
1074 220 119 63
BsrFI r'CCGG_y
Cuts at: 0 149 1476
Size: 149 1327
BstEII G'GTnAC_C
Cuts at: 0 517 1440 1476
Size: 517 923 36
Fragments arranged by size:
923 517 36
BstYI r'GATC_y
Cuts at: 0 1224 1476
Size: 1224 252
Bsu36I CC'TnA_GG
Cuts at: 0 1123 1476
Size: 1123 353
BtgI C'CryG_G
Cuts at: 0 86 1211 1476
Size: 86 1125 265
Fragments arranged by size:
1125 265 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1289 1377 1476
Size: 1289 88 99
Fragments arranged by size:
1289 99 88
BtsI GCAGTG_nn'
Cuts at: 0 1046 1111 1410 1476
Size: 1046 65 299 66
Fragments arranged by size:
1046 299 66 65
DraI TTT'AAA
Cuts at: 0 1455 1476
Size: 1455 21
EaeI y'GGCC_r
Cuts at: 0 58 204 1476
Size: 58 146 1272
Fragments arranged by size:
1272 146 58
EagI C'GGCC_G
Cuts at: 0 58 204 1476
Size: 58 146 1272
Fragments arranged by size:
1272 146 58
EcoICRI GAG'CTC
Cuts at: 0 600 1476
Size: 600 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 434 650 675 734 1259 1476
Size: 55 379 216 25 59 525 217
Fragments arranged by size:
525 379 217 216 59 55 25
EcoO109I rG'GnC_Cy
Cuts at: 0 53 72 793 871 1177 1178 1476
Size: 53 19 721 78 306 1 298
Fragments arranged by size:
721 306 298 78 53 19 1
EcoRV GAT'ATC
Cuts at: 0 1335 1476
Size: 1335 141
HaeII r_GCGC'y
Cuts at: 0 34 126 1476
Size: 34 92 1350
Fragments arranged by size:
1350 92 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 303 335 489 521 734 766 1476
Size: 303 32 154 32 213 32 710
Fragments arranged by size:
710 303 213 154 32 32 32
HincII GTy'rAC
Cuts at: 0 942 1476
Size: 942 534
HindIII A'AGCT_T
Cuts at: 0 448 706 1457 1476
Size: 448 258 751 19
Fragments arranged by size:
751 448 258 19
MspA1I CmG'CkG
Cuts at: 0 132 842 936 1476
Size: 132 710 94 540
Fragments arranged by size:
710 540 132 94
NaeI GCC'GGC
Cuts at: 0 151 1476
Size: 151 1325
NcoI C'CATG_G
Cuts at: 0 1211 1476
Size: 1211 265
NgoMIV G'CCGG_C
Cuts at: 0 149 1476
Size: 149 1327
NruI TCG'CGA
Cuts at: 0 1396 1476
Size: 1396 80
NspI r_CATG'y
Cuts at: 0 1090 1476
Size: 1090 386
PasI CC'CwG_GG
Cuts at: 0 867 1476
Size: 867 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 1350 1476
Size: 1350 126
PfoI T'CCnGG_A
Cuts at: 0 1227 1476
Size: 1227 249
PpuMI rG'GwC_Cy
Cuts at: 0 72 1476
Size: 72 1404
PspOMI G'GGCC_C
Cuts at: 0 52 1177 1476
Size: 52 1125 299
Fragments arranged by size:
1125 299 52
PspXI vC'TCGA_Gb
Cuts at: 0 1034 1476
Size: 1034 442
PstI C_TGCA'G
Cuts at: 0 1431 1476
Size: 1431 45
PvuII CAG'CTG
Cuts at: 0 842 936 1476
Size: 842 94 540
Fragments arranged by size:
842 540 94
SacI G_AGCT'C
Cuts at: 0 602 1476
Size: 602 874
SexAI A'CCwGG_T
Cuts at: 0 74 521 1139 1476
Size: 74 447 618 337
Fragments arranged by size:
618 447 337 74
SfcI C'TryA_G
Cuts at: 0 837 1427 1476
Size: 837 590 49
Fragments arranged by size:
837 590 49
SgrAI Cr'CCGG_yG
Cuts at: 0 149 1476
Size: 149 1327
SmaI CCC'GGG
Cuts at: 0 28 70 1476
Size: 28 42 1406
Fragments arranged by size:
1406 42 28
SmlI C'TyrA_G
Cuts at: 0 253 546 1034 1201 1476
Size: 253 293 488 167 275
Fragments arranged by size:
488 293 275 253 167
SrfI GCCC'GGGC
Cuts at: 0 28 1476
Size: 28 1448
SspI AAT'ATT
Cuts at: 0 1062 1476
Size: 1062 414
StuI AGG'CCT
Cuts at: 0 419 1476
Size: 419 1057
StyI C'CwwG_G
Cuts at: 0 431 796 1164 1211 1476
Size: 431 365 368 47 265
Fragments arranged by size:
431 368 365 265 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 834 1390 1404 1476
Size: 834 556 14 72
Fragments arranged by size:
834 556 72 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 1476
Size: 1218 258
XhoI C'TCGA_G
Cuts at: 0 1034 1476
Size: 1034 442
XmaI C'CCGG_G
Cuts at: 0 26 68 1476
Size: 26 42 1408
Fragments arranged by size:
1408 42 26
XmnI GAAnn'nnTTC
Cuts at: 0 272 419 1476
Size: 272 147 1057
Fragments arranged by size:
1057 272 147
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BcgI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EagI EcoICRI Eco57MI EcoO109I
EcoRV HaeII Hin4I HincII HindIII MspA1I NaeI NcoI
NgoMIV NruI NspI PasI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SexAI SfcI SgrAI SmaI
SmlI SrfI SspI StuI StyI TaqII XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AleI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
Bpu10I BsaWI BsiWI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI DraIII
DrdI EarI EciI EcoNI EcoRI FalI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NarI NdeI NheI NotI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SnaBI SpeI SphI SwaI TatI TstI Tth111I
XbaI ZraI