Restriction map for
pF1KE1067
(Linear) (Six-base) MAPSORT of: KIEE1067.seq Check: 2732 from: 1 to: 1518
KIEE1067.orf
With 182 enzymes: SgfI *
February 15, 2010 17:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1053 1518
Size: 1053 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 369 405 970 1102 1518
Size: 274 95 36 565 132 416
Fragments arranged by size:
565 416 274 132 95 36
AhdI GACnn_n'nnGTC
Cuts at: 0 1434 1518
Size: 1434 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 551 1518
Size: 551 967
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 1518
Size: 1485 33
ApoI r'AATT_y
Cuts at: 0 694 1518
Size: 694 824
AvaI C'yCGr_G
Cuts at: 0 398 1306 1518
Size: 398 908 212
Fragments arranged by size:
908 398 212
BamHI G'GATC_C
Cuts at: 0 50 1518
Size: 50 1468
BanI G'GyrC_C
Cuts at: 0 406 1518
Size: 406 1112
BanII G_rGCy'C
Cuts at: 0 782 1111 1152 1361 1518
Size: 782 329 41 209 157
Fragments arranged by size:
782 329 209 157 41
BbvCI CC'TCA_GC
Cuts at: 0 968 1518
Size: 968 550
BciVI GTATCCnnnnn_n'
Cuts at: 0 164 1518
Size: 164 1354
BglI GCCn_nnn'nGGC
Cuts at: 0 619 628 1518
Size: 619 9 890
Fragments arranged by size:
890 619 9
BglII A'GATC_T
Cuts at: 0 380 1518
Size: 380 1138
BlpI GC'TnA_GC
Cuts at: 0 511 1518
Size: 511 1007
Bme1580I G_kGCm'C
Cuts at: 0 41 847 1518
Size: 41 806 671
Fragments arranged by size:
806 671 41
BmgBI CAC'GTC
Cuts at: 0 123 299 1518
Size: 123 176 1219
Fragments arranged by size:
1219 176 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 1495 1518
Size: 1495 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 639 1446 1518
Size: 639 807 72
Fragments arranged by size:
807 639 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 818 871 883 1006 1021 1147 1303
Size: 629 189 53 12 123 15 126 156
Cuts at: 1303 1375 1518
Size: 72 143
Fragments arranged by size:
629 189 156 143 126 123 72 53
15 12
Bpu10I CC'TnA_GC
Cuts at: 0 582 968 1181 1518
Size: 582 386 213 337
Fragments arranged by size:
582 386 337 213
BsaI GGTCTCn'nnnn_
Cuts at: 0 581 1518
Size: 581 937
BsaAI yAC'GTr
Cuts at: 0 108 1518
Size: 108 1410
BsaHI Gr'CG_yC
Cuts at: 0 71 1518
Size: 71 1447
BsaWI w'CCGG_w
Cuts at: 0 1144 1518
Size: 1144 374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 739 769 1384 1414 1518
Size: 230 30 479 30 615 30 104
Fragments arranged by size:
615 479 230 104 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 874 886 1435 1459 1518
Size: 655 219 12 549 24 59
Fragments arranged by size:
655 549 219 59 24 12
BseYI C'CCAG_C
Cuts at: 0 1209 1323 1518
Size: 1209 114 195
Fragments arranged by size:
1209 195 114
BsiHKAI G_wGCw'C
Cuts at: 0 1163 1361 1518
Size: 1163 198 157
Fragments arranged by size:
1163 198 157
BsmI GAATG_Cn'
Cuts at: 0 540 1197 1518
Size: 540 657 321
Fragments arranged by size:
657 540 321
Bsp1286I G_dGCh'C
Cuts at: 0 41 782 847 1111 1152 1163 1361 1518
Size: 41 741 65 264 41 11 198 157
Fragments arranged by size:
741 264 198 157 65 41 41 11
BspEI T'CCGG_A
Cuts at: 0 1144 1518
Size: 1144 374
BspHI T'CATG_A
Cuts at: 0 360 1518
Size: 360 1158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 1053 1202 1518
Size: 53 1000 149 316
Fragments arranged by size:
1000 316 149 53
BsrBI CCG'CTC
Cuts at: 0 1142 1518
Size: 1142 376
BssSI C'ACGA_G
Cuts at: 0 1431 1518
Size: 1431 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 1518
Size: 45 1473
BstXI CCAn_nnnn'nTGG
Cuts at: 0 553 607 1518
Size: 553 54 911
Fragments arranged by size:
911 553 54
BstYI r'GATC_y
Cuts at: 0 50 344 380 1518
Size: 50 294 36 1138
Fragments arranged by size:
1138 294 50 36
Bsu36I CC'TnA_GG
Cuts at: 0 1254 1518
Size: 1254 264
BtgI C'CryG_G
Cuts at: 0 626 1103 1518
Size: 626 477 415
Fragments arranged by size:
626 477 415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1368 1518
Size: 1368 150
BtsI GCAGTG_nn'
Cuts at: 0 632 1518
Size: 632 886
ClaI AT'CG_AT
Cuts at: 0 653 1518
Size: 653 865
DraI TTT'AAA
Cuts at: 0 186 438 1518
Size: 186 252 1080
Fragments arranged by size:
1080 252 186
DrdI GACnn_nn'nnGTC
Cuts at: 0 858 1518
Size: 858 660
EaeI y'GGCC_r
Cuts at: 0 543 629 1518
Size: 543 86 889
Fragments arranged by size:
889 543 86
EarI CTCTTCn'nnn_
Cuts at: 0 445 1518
Size: 445 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1292 1518
Size: 1292 226
EcoICRI GAG'CTC
Cuts at: 0 1359 1518
Size: 1359 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 369 405 629 818 871 883 970
Size: 274 95 36 224 189 53 12 87
Cuts at: 970 1006 1021 1102 1147 1303 1375 1518
Size: 36 15 81 45 156 72 143
Fragments arranged by size:
274 224 189 156 143 95 87 81
72 53 45 36 36 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 798 1257 1518
Size: 798 459 261
Fragments arranged by size:
798 459 261
EcoO109I rG'GnC_Cy
Cuts at: 0 330 620 666 1191 1518
Size: 330 290 46 525 327
Fragments arranged by size:
525 330 327 290 46
EcoRI G'AATT_C
Cuts at: 0 694 1518
Size: 694 824
HaeII r_GCGC'y
Cuts at: 0 1342 1518
Size: 1342 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 1420 1452 1518
Size: 1384 32 4 32 66
Fragments arranged by size:
1384 66 32 32 4
HindIII A'AGCT_T
Cuts at: 0 268 1518
Size: 268 1250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 1518
Size: 1427 91
MscI TGG'CCA
Cuts at: 0 545 1518
Size: 545 973
MspA1I CmG'CkG
Cuts at: 0 858 1209 1518
Size: 858 351 309
Fragments arranged by size:
858 351 309
NcoI C'CATG_G
Cuts at: 0 1103 1518
Size: 1103 415
NspI r_CATG'y
Cuts at: 0 1035 1518
Size: 1035 483
PfoI T'CCnGG_A
Cuts at: 0 689 1518
Size: 689 829
PpuMI rG'GwC_Cy
Cuts at: 0 330 666 1191 1518
Size: 330 336 525 327
Fragments arranged by size:
525 336 330 327
PstI C_TGCA'G
Cuts at: 0 521 639 1022 1475 1518
Size: 521 118 383 453 43
Fragments arranged by size:
521 453 383 118 43
SacI G_AGCT'C
Cuts at: 0 1361 1518
Size: 1361 157
SapI GCTCTTCn'nnn_
Cuts at: 0 445 1518
Size: 445 1073
SfcI C'TryA_G
Cuts at: 0 339 517 635 725 1018 1471 1518
Size: 339 178 118 90 293 453 47
Fragments arranged by size:
453 339 293 178 118 90 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 628 1518
Size: 628 890
SmlI C'TyrA_G
Cuts at: 0 398 1518
Size: 398 1120
SnaBI TAC'GTA
Cuts at: 0 108 1518
Size: 108 1410
StuI AGG'CCT
Cuts at: 0 490 839 1518
Size: 490 349 679
Fragments arranged by size:
679 490 349
StyI C'CwwG_G
Cuts at: 0 127 758 1103 1518
Size: 127 631 345 415
Fragments arranged by size:
631 415 345 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1518
Size: 813 705
TatI w'GTAC_w
Cuts at: 0 17 1518
Size: 17 1501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 230 262 283 315 739 771 1518
Size: 230 32 21 32 424 32 747
Fragments arranged by size:
747 424 230 32 32 32 21
XbaI T'CTAG_A
Cuts at: 0 717 1518
Size: 717 801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 1518
Size: 141 1377
XhoI C'TCGA_G
Cuts at: 0 398 1518
Size: 398 1120
XmnI GAAnn'nnTTC
Cuts at: 0 382 1518
Size: 382 1136
Enzymes that do cut:
AarI AcuI AhdI AlfI AlwNI ApoI AvaI BamHI
BanI BanII BbvCI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HindIII MmeI MscI MspA1I NcoI NspI PfoI PpuMI
PstI SacI SapI SfcI SfiI SmlI SnaBI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BclI BfrBI BmtI BpuEI
BsaBI BsgI BsiEI BsiWI BsmBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I CspCI DraIII EagI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
SwaI Tth111I XmaI ZraI